Kazachstania africana (strain ATCC 22294 / BCRC 22015 / CBS 2517 / CECT 1963 / NBRC 1671 / NRRL Y-8276) (Yeast) (Kluyveromyces africanus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Kazachstania; Kazachstania africana

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5354 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H2AS48|H2AS48_KAZAF Uncharacterized protein OS=Kazachstania africana (strain ATCC 22294 / BCRC 22015 / CBS 2517 / CECT 1963 / NBRC 1671 / NRRL Y-8276) OX=1071382 GN=KAFR0C02050 PE=4 SV=1
MM1 pKa = 7.3QLQDD5 pKa = 4.56LLLSLSVAISTAHH18 pKa = 6.2AWSPTDD24 pKa = 3.13SYY26 pKa = 12.13APGEE30 pKa = 4.31ITCDD34 pKa = 3.15ADD36 pKa = 3.6VNLVRR41 pKa = 11.84NATDD45 pKa = 3.66LSSDD49 pKa = 3.42EE50 pKa = 4.21QEE52 pKa = 3.96WLKK55 pKa = 11.11KK56 pKa = 9.96RR57 pKa = 11.84DD58 pKa = 3.99TITEE62 pKa = 4.09SAMKK66 pKa = 9.93TFLTRR71 pKa = 11.84ATANFSDD78 pKa = 3.79TSFLDD83 pKa = 3.55SLFEE87 pKa = 4.09NSSYY91 pKa = 10.67VPRR94 pKa = 11.84VGIAASGGGYY104 pKa = 9.96RR105 pKa = 11.84AMLSGAGMLAAFDD118 pKa = 3.62NRR120 pKa = 11.84TRR122 pKa = 11.84GANEE126 pKa = 3.71NGLGGLLQATTYY138 pKa = 11.11LSGLSGGNWMTGTLAWNNWTSVQEE162 pKa = 4.46IIDD165 pKa = 4.14SFSDD169 pKa = 3.72SNTSSIWDD177 pKa = 3.38ITNSIVTPGGNNSTFTSEE195 pKa = 3.77RR196 pKa = 11.84FASIISDD203 pKa = 3.15IDD205 pKa = 3.69DD206 pKa = 3.73KK207 pKa = 11.81QEE209 pKa = 3.77AGFDD213 pKa = 3.33ITLVDD218 pKa = 2.95VWGRR222 pKa = 11.84MLSYY226 pKa = 11.06YY227 pKa = 9.51FFPSLPNGGAAYY239 pKa = 8.0TWSTLRR245 pKa = 11.84DD246 pKa = 2.92ADD248 pKa = 3.54VFMNAEE254 pKa = 4.15MPFPISVADD263 pKa = 3.81GQYY266 pKa = 10.4PGTSVDD272 pKa = 3.88SLNSTVFEE280 pKa = 4.22FNPFEE285 pKa = 4.28MGSWDD290 pKa = 3.35PTLNAFTDD298 pKa = 3.73VKK300 pKa = 11.23YY301 pKa = 10.95LGTNVTNGSPIEE313 pKa = 4.17SGKK316 pKa = 10.77CIAGFDD322 pKa = 3.59NVGFIIGTSSDD333 pKa = 3.17ILNDD337 pKa = 2.99ITSSYY342 pKa = 10.42EE343 pKa = 3.66AVLTYY348 pKa = 10.93LEE350 pKa = 4.61TEE352 pKa = 4.31FLSDD356 pKa = 3.24STATSDD362 pKa = 3.45EE363 pKa = 4.41YY364 pKa = 11.48TLGIYY369 pKa = 10.17SPNPFKK375 pKa = 10.78GTTNIDD381 pKa = 3.55SNYY384 pKa = 8.02STSIVDD390 pKa = 3.56SDD392 pKa = 4.06SLYY395 pKa = 11.02LADD398 pKa = 4.44GGEE401 pKa = 4.5DD402 pKa = 3.88GEE404 pKa = 4.71VVPFIPLIKK413 pKa = 9.83KK414 pKa = 8.9EE415 pKa = 4.04RR416 pKa = 11.84EE417 pKa = 3.54IDD419 pKa = 3.65VVFAFDD425 pKa = 4.77NDD427 pKa = 4.3DD428 pKa = 5.73GSDD431 pKa = 3.65DD432 pKa = 3.86WPSGSNLISTYY443 pKa = 9.28EE444 pKa = 4.0RR445 pKa = 11.84QFSSQGKK452 pKa = 9.39SIAFPYY458 pKa = 10.59VPDD461 pKa = 4.22LATFSDD467 pKa = 3.7LGLNEE472 pKa = 4.14RR473 pKa = 11.84PTFFGCDD480 pKa = 3.14ASNLTDD486 pKa = 4.16LAYY489 pKa = 10.38VPPLIVYY496 pKa = 9.49IPNAQYY502 pKa = 10.89SYY504 pKa = 10.94PSNTSTYY511 pKa = 7.89TLSYY515 pKa = 11.09SMEE518 pKa = 3.94EE519 pKa = 3.87RR520 pKa = 11.84LEE522 pKa = 4.23MIQNGYY528 pKa = 8.97EE529 pKa = 3.61AATRR533 pKa = 11.84NNLTEE538 pKa = 4.36DD539 pKa = 3.77SGFLGCVGCAVIRR552 pKa = 11.84RR553 pKa = 11.84KK554 pKa = 9.83QEE556 pKa = 3.83SLNITLPSEE565 pKa = 4.5CEE567 pKa = 3.63QCFANYY573 pKa = 9.07CWNGTLTDD581 pKa = 3.71VSSVSSSNSSYY592 pKa = 11.61SNSTTTLSSGAAEE605 pKa = 4.2STTASSNASNSSSTSSSSAASSSSAAKK632 pKa = 10.35ANAAASVEE640 pKa = 4.23TGKK643 pKa = 10.77LFSLLTAFGVLFSLMM658 pKa = 3.86

Molecular weight: 70.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H2B2C9|H2B2C9_KAZAF SEC7 domain-containing protein OS=Kazachstania africana (strain ATCC 22294 / BCRC 22015 / CBS 2517 / CECT 1963 / NBRC 1671 / NRRL Y-8276) OX=1071382 GN=KAFR0L01690 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.71RR15 pKa = 11.84KK16 pKa = 8.27RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5354

0

5354

2609361

25

4933

487.4

55.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.193 ± 0.024

1.18 ± 0.011

5.985 ± 0.022

6.705 ± 0.037

4.554 ± 0.019

4.698 ± 0.03

2.037 ± 0.012

6.994 ± 0.025

7.409 ± 0.034

9.724 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.061 ± 0.01

6.577 ± 0.032

3.993 ± 0.02

3.761 ± 0.021

4.273 ± 0.021

8.912 ± 0.049

5.952 ± 0.033

5.525 ± 0.023

1.008 ± 0.01

3.459 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski