Clostridium novyi (strain NT)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium novyi

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2305 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|A0Q2T1|CH60_CLONN 60 kDa chaperonin OS=Clostridium novyi (strain NT) OX=386415 GN=groL PE=3 SV=1
MM1 pKa = 6.91EE2 pKa = 5.83TINEE6 pKa = 4.33KK7 pKa = 10.38LDD9 pKa = 3.36YY10 pKa = 9.84MYY12 pKa = 11.43DD13 pKa = 3.97DD14 pKa = 3.94IFSNDD19 pKa = 3.32EE20 pKa = 3.98YY21 pKa = 11.79VGDD24 pKa = 4.2KK25 pKa = 11.1NNEE28 pKa = 3.91AFDD31 pKa = 4.36KK32 pKa = 11.02FDD34 pKa = 5.38DD35 pKa = 3.97EE36 pKa = 5.24LKK38 pKa = 10.59FYY40 pKa = 10.82YY41 pKa = 10.5

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A0PXW0|RS8_CLONN 30S ribosomal protein S8 OS=Clostridium novyi (strain NT) OX=386415 GN=rpsH PE=3 SV=1
MM1 pKa = 7.33ARR3 pKa = 11.84KK4 pKa = 9.96AMIEE8 pKa = 3.94KK9 pKa = 9.25WKK11 pKa = 9.17KK12 pKa = 6.52TPKK15 pKa = 9.86YY16 pKa = 8.38STRR19 pKa = 11.84AYY21 pKa = 8.1TRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 7.08SVLKK35 pKa = 10.64KK36 pKa = 10.27YY37 pKa = 9.54GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.7LAYY49 pKa = 10.44RR50 pKa = 11.84GQIPGCKK57 pKa = 8.74KK58 pKa = 10.74ASWW61 pKa = 3.34

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2305

0

2305

729213

30

2861

316.4

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.411 ± 0.063

1.247 ± 0.019

5.558 ± 0.043

7.443 ± 0.064

4.276 ± 0.04

6.314 ± 0.05

1.356 ± 0.018

10.089 ± 0.063

9.962 ± 0.062

8.736 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.627 ± 0.027

6.594 ± 0.054

2.782 ± 0.029

2.242 ± 0.023

3.306 ± 0.035

6.036 ± 0.037

4.736 ± 0.03

6.642 ± 0.048

0.604 ± 0.015

4.039 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski