Firmicutes bacterium CAG:534

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2499 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6ZSH6|R6ZSH6_9FIRM Radical SAM domain protein OS=Firmicutes bacterium CAG:534 OX=1263027 GN=BN699_01868 PE=4 SV=1
MM1 pKa = 7.66TILAQRR7 pKa = 11.84KK8 pKa = 6.71KK9 pKa = 8.63TAALLFLTVLFLTAFLSGCGKK30 pKa = 10.18KK31 pKa = 10.49DD32 pKa = 3.35PALEE36 pKa = 4.04NYY38 pKa = 8.15KK39 pKa = 11.27ANMTQFFEE47 pKa = 4.06NMKK50 pKa = 10.31VFDD53 pKa = 4.05SSINAIDD60 pKa = 4.54PNSDD64 pKa = 3.32TAPAQLLALLDD75 pKa = 3.76NMNTSFEE82 pKa = 4.15QMASLEE88 pKa = 4.35VPDD91 pKa = 5.39DD92 pKa = 3.95FPGVAEE98 pKa = 4.85LATQASEE105 pKa = 4.07NMSQAVYY112 pKa = 10.05CYY114 pKa = 10.0HH115 pKa = 6.34QAFDD119 pKa = 3.76GDD121 pKa = 4.16TYY123 pKa = 10.24NANMADD129 pKa = 3.74AAKK132 pKa = 10.15QYY134 pKa = 10.92YY135 pKa = 10.33DD136 pKa = 3.59RR137 pKa = 11.84ANLRR141 pKa = 11.84IQYY144 pKa = 9.68IISILHH150 pKa = 5.52GNIPEE155 pKa = 4.56EE156 pKa = 4.0IYY158 pKa = 10.77SYY160 pKa = 11.48DD161 pKa = 3.7EE162 pKa = 4.17EE163 pKa = 4.87TPSDD167 pKa = 3.88TSQEE171 pKa = 4.26TAPSDD176 pKa = 3.54PSTEE180 pKa = 4.15EE181 pKa = 3.97SSSDD185 pKa = 4.27DD186 pKa = 4.74NFDD189 pKa = 3.43TDD191 pKa = 3.8DD192 pKa = 3.52TVFYY196 pKa = 10.58DD197 pKa = 4.58DD198 pKa = 4.55EE199 pKa = 5.84SSGEE203 pKa = 4.16DD204 pKa = 3.36VSEE207 pKa = 4.18EE208 pKa = 3.88

Molecular weight: 23.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6ZR50|R6ZR50_9FIRM Predicted branched-chain amino acid permease (Azaleucine resistance) OS=Firmicutes bacterium CAG:534 OX=1263027 GN=BN699_01428 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.63NRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.89VHH16 pKa = 5.88GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2499

0

2499

794839

29

4120

318.1

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.608 ± 0.055

1.548 ± 0.021

5.295 ± 0.041

8.003 ± 0.063

4.224 ± 0.04

7.315 ± 0.04

1.694 ± 0.022

7.097 ± 0.039

6.743 ± 0.044

9.462 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.132 ± 0.025

3.881 ± 0.036

3.264 ± 0.025

3.48 ± 0.028

4.519 ± 0.041

5.655 ± 0.037

5.111 ± 0.038

6.765 ± 0.041

0.898 ± 0.02

4.303 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski