Amino acid dipepetide frequency for Firmicutes bacterium CAG:534

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.61AlaAla: 7.61 ± 0.14	1.181AlaCys: 1.181 ± 0.041	4.48AlaAsp: 4.48 ± 0.084	5.474AlaGlu: 5.474 ± 0.095	3.348AlaPhe: 3.348 ± 0.067	6.609AlaGly: 6.609 ± 0.109	1.071AlaHis: 1.071 ± 0.039	4.718AlaIle: 4.718 ± 0.093	4.681AlaLys: 4.681 ± 0.089	7.279AlaLeu: 7.279 ± 0.124	2.359AlaMet: 2.359 ± 0.052	2.248AlaAsn: 2.248 ± 0.069	2.082AlaPro: 2.082 ± 0.06	2.705AlaGln: 2.705 ± 0.065	3.045AlaArg: 3.045 ± 0.064	3.996AlaSer: 3.996 ± 0.072	3.071AlaThr: 3.071 ± 0.066	6.479AlaVal: 6.479 ± 0.115	0.533AlaTrp: 0.533 ± 0.025	3.108AlaTyr: 3.108 ± 0.066	0.004AlaXaa: 0.004 ± 0.002
Cys	1.052CysAla: 1.052 ± 0.036	0.32CysCys: 0.32 ± 0.023	0.769CysAsp: 0.769 ± 0.032	0.952CysGlu: 0.952 ± 0.037	0.829CysPhe: 0.829 ± 0.035	1.58CysGly: 1.58 ± 0.053	0.354CysHis: 0.354 ± 0.022	1.092CysIle: 1.092 ± 0.035	0.835CysLys: 0.835 ± 0.04	1.331CysLeu: 1.331 ± 0.046	0.505CysMet: 0.505 ± 0.025	0.58CysAsn: 0.58 ± 0.03	0.581CysPro: 0.581 ± 0.03	0.432CysGln: 0.432 ± 0.027	0.834CysArg: 0.834 ± 0.033	0.979CysSer: 0.979 ± 0.039	0.655CysThr: 0.655 ± 0.029	1.042CysVal: 1.042 ± 0.038	0.111CysTrp: 0.111 ± 0.013	0.645CysTyr: 0.645 ± 0.032	0.0CysXaa: 0.0 ± 0.0
Asp	4.002AspAla: 4.002 ± 0.081	0.757AspCys: 0.757 ± 0.033	2.35AspAsp: 2.35 ± 0.07	4.094AspGlu: 4.094 ± 0.078	2.565AspPhe: 2.565 ± 0.064	4.022AspGly: 4.022 ± 0.075	1.005AspHis: 1.005 ± 0.036	3.82AspIle: 3.82 ± 0.083	3.294AspLys: 3.294 ± 0.07	4.681AspLeu: 4.681 ± 0.084	1.759AspMet: 1.759 ± 0.049	1.851AspAsn: 1.851 ± 0.06	1.987AspPro: 1.987 ± 0.053	1.854AspGln: 1.854 ± 0.047	2.559AspArg: 2.559 ± 0.057	2.544AspSer: 2.544 ± 0.066	2.897AspThr: 2.897 ± 0.075	3.471AspVal: 3.471 ± 0.069	0.533AspTrp: 0.533 ± 0.03	2.902AspTyr: 2.902 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	6.073GluAla: 6.073 ± 0.105	0.82GluCys: 0.82 ± 0.033	4.328GluAsp: 4.328 ± 0.091	8.609GluGlu: 8.609 ± 0.151	2.52GluPhe: 2.52 ± 0.066	5.236GluGly: 5.236 ± 0.096	1.312GluHis: 1.312 ± 0.045	5.729GluIle: 5.729 ± 0.088	7.449GluLys: 7.449 ± 0.12	7.37GluLeu: 7.37 ± 0.108	2.567GluMet: 2.567 ± 0.058	3.944GluAsn: 3.944 ± 0.076	1.916GluPro: 1.916 ± 0.055	3.374GluGln: 3.374 ± 0.082	3.589GluArg: 3.589 ± 0.078	3.187GluSer: 3.187 ± 0.069	3.763GluThr: 3.763 ± 0.07	4.475GluVal: 4.475 ± 0.094	0.633GluTrp: 0.633 ± 0.029	3.134GluTyr: 3.134 ± 0.075	0.0GluXaa: 0.0 ± 0.0
Phe	3.279PheAla: 3.279 ± 0.059	0.896PheCys: 0.896 ± 0.04	2.402PheAsp: 2.402 ± 0.06	2.812PheGlu: 2.812 ± 0.051	2.087PhePhe: 2.087 ± 0.063	3.142PheGly: 3.142 ± 0.077	0.903PheHis: 0.903 ± 0.033	2.536PheIle: 2.536 ± 0.065	1.867PheLys: 1.867 ± 0.045	4.788PheLeu: 4.788 ± 0.112	1.237PheMet: 1.237 ± 0.041	1.269PheAsn: 1.269 ± 0.041	1.423PhePro: 1.423 ± 0.046	1.453PheGln: 1.453 ± 0.046	1.809PheArg: 1.809 ± 0.053	2.745PheSer: 2.745 ± 0.062	2.243PheThr: 2.243 ± 0.058	2.953PheVal: 2.953 ± 0.066	0.447PheTrp: 0.447 ± 0.027	1.948PheTyr: 1.948 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	5.246GlyAla: 5.246 ± 0.109	1.341GlyCys: 1.341 ± 0.044	3.398GlyAsp: 3.398 ± 0.076	5.397GlyGlu: 5.397 ± 0.089	3.214GlyPhe: 3.214 ± 0.068	4.751GlyGly: 4.751 ± 0.09	1.256GlyHis: 1.256 ± 0.04	6.398GlyIle: 6.398 ± 0.096	5.723GlyLys: 5.723 ± 0.104	6.072GlyLeu: 6.072 ± 0.099	2.724GlyMet: 2.724 ± 0.062	3.154GlyAsn: 3.154 ± 0.077	1.303GlyPro: 1.303 ± 0.038	2.505GlyGln: 2.505 ± 0.056	3.376GlyArg: 3.376 ± 0.072	4.264GlySer: 4.264 ± 0.09	4.083GlyThr: 4.083 ± 0.078	4.782GlyVal: 4.782 ± 0.088	0.678GlyTrp: 0.678 ± 0.034	3.481GlyTyr: 3.481 ± 0.068	0.004GlyXaa: 0.004 ± 0.002
His	1.057HisAla: 1.057 ± 0.035	0.277HisCys: 0.277 ± 0.018	0.897HisAsp: 0.897 ± 0.036	1.02HisGlu: 1.02 ± 0.038	0.866HisPhe: 0.866 ± 0.032	1.266HisGly: 1.266 ± 0.041	0.405HisHis: 0.405 ± 0.033	1.336HisIle: 1.336 ± 0.05	1.033HisLys: 1.033 ± 0.035	1.471HisLeu: 1.471 ± 0.044	0.561HisMet: 0.561 ± 0.024	0.658HisAsn: 0.658 ± 0.03	0.883HisPro: 0.883 ± 0.035	0.503HisGln: 0.503 ± 0.024	0.801HisArg: 0.801 ± 0.033	0.931HisSer: 0.931 ± 0.035	0.876HisThr: 0.876 ± 0.036	1.105HisVal: 1.105 ± 0.039	0.145HisTrp: 0.145 ± 0.015	0.854HisTyr: 0.854 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.433IleAla: 5.433 ± 0.091	1.413IleCys: 1.413 ± 0.038	3.651IleAsp: 3.651 ± 0.076	4.646IleGlu: 4.646 ± 0.084	2.831IlePhe: 2.831 ± 0.071	5.046IleGly: 5.046 ± 0.087	1.248IleHis: 1.248 ± 0.039	4.527IleIle: 4.527 ± 0.09	4.044IleLys: 4.044 ± 0.079	7.439IleLeu: 7.439 ± 0.137	1.992IleMet: 1.992 ± 0.053	2.718IleAsn: 2.718 ± 0.062	3.077IlePro: 3.077 ± 0.071	2.104IleGln: 2.104 ± 0.057	3.694IleArg: 3.694 ± 0.073	4.889IleSer: 4.889 ± 0.079	4.251IleThr: 4.251 ± 0.078	4.424IleVal: 4.424 ± 0.081	0.711IleTrp: 0.711 ± 0.03	2.836IleTyr: 2.836 ± 0.061	0.001IleXaa: 0.001 ± 0.001
Lys	5.207LysAla: 5.207 ± 0.093	0.715LysCys: 0.715 ± 0.028	3.64LysAsp: 3.64 ± 0.071	7.453LysGlu: 7.453 ± 0.116	1.702LysPhe: 1.702 ± 0.044	4.728LysGly: 4.728 ± 0.084	0.911LysHis: 0.911 ± 0.037	4.575LysIle: 4.575 ± 0.079	5.917LysLys: 5.917 ± 0.1	5.664LysLeu: 5.664 ± 0.088	2.12LysMet: 2.12 ± 0.05	3.081LysAsn: 3.081 ± 0.067	1.924LysPro: 1.924 ± 0.055	2.53LysGln: 2.53 ± 0.053	3.262LysArg: 3.262 ± 0.075	3.077LysSer: 3.077 ± 0.068	3.423LysThr: 3.423 ± 0.058	4.139LysVal: 4.139 ± 0.072	0.696LysTrp: 0.696 ± 0.034	2.665LysTyr: 2.665 ± 0.062	0.0LysXaa: 0.0 ± 0.0
Leu	7.008LeuAla: 7.008 ± 0.106	1.851LeuCys: 1.851 ± 0.046	4.621LeuAsp: 4.621 ± 0.089	6.459LeuGlu: 6.459 ± 0.113	4.693LeuPhe: 4.693 ± 0.111	6.136LeuGly: 6.136 ± 0.1	1.623LeuHis: 1.623 ± 0.045	6.231LeuIle: 6.231 ± 0.125	6.137LeuLys: 6.137 ± 0.096	10.39LeuLeu: 10.39 ± 0.177	2.7LeuMet: 2.7 ± 0.063	3.337LeuAsn: 3.337 ± 0.073	3.738LeuPro: 3.738 ± 0.074	3.252LeuGln: 3.252 ± 0.06	3.854LeuArg: 3.854 ± 0.088	7.076LeuSer: 7.076 ± 0.117	5.197LeuThr: 5.197 ± 0.099	5.595LeuVal: 5.595 ± 0.091	0.881LeuTrp: 0.881 ± 0.037	3.842LeuTyr: 3.842 ± 0.084	0.001LeuXaa: 0.001 ± 0.001
Met	2.575MetAla: 2.575 ± 0.063	0.365MetCys: 0.365 ± 0.022	1.812MetAsp: 1.812 ± 0.048	2.835MetGlu: 2.835 ± 0.067	0.879MetPhe: 0.879 ± 0.037	2.282MetGly: 2.282 ± 0.053	0.468MetHis: 0.468 ± 0.024	2.319MetIle: 2.319 ± 0.052	2.583MetLys: 2.583 ± 0.057	2.882MetLeu: 2.882 ± 0.067	0.912MetMet: 0.912 ± 0.043	1.39MetAsn: 1.39 ± 0.041	1.179MetPro: 1.179 ± 0.036	1.142MetGln: 1.142 ± 0.034	1.243MetArg: 1.243 ± 0.043	1.656MetSer: 1.656 ± 0.053	1.663MetThr: 1.663 ± 0.047	2.014MetVal: 2.014 ± 0.059	0.215MetTrp: 0.215 ± 0.016	0.901MetTyr: 0.901 ± 0.034	0.001MetXaa: 0.001 ± 0.001
Asn	2.91AsnAla: 2.91 ± 0.074	0.585AsnCys: 0.585 ± 0.028	1.808AsnAsp: 1.808 ± 0.049	2.563AsnGlu: 2.563 ± 0.056	1.481AsnPhe: 1.481 ± 0.043	3.252AsnGly: 3.252 ± 0.075	0.718AsnHis: 0.718 ± 0.028	2.886AsnIle: 2.886 ± 0.069	2.286AsnLys: 2.286 ± 0.063	3.545AsnLeu: 3.545 ± 0.068	1.352AsnMet: 1.352 ± 0.044	1.498AsnAsn: 1.498 ± 0.052	1.661AsnPro: 1.661 ± 0.044	1.329AsnGln: 1.329 ± 0.04	1.97AsnArg: 1.97 ± 0.047	2.036AsnSer: 2.036 ± 0.066	2.077AsnThr: 2.077 ± 0.064	2.734AsnVal: 2.734 ± 0.069	0.428AsnTrp: 0.428 ± 0.023	1.695AsnTyr: 1.695 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	2.515ProAla: 2.515 ± 0.06	0.439ProCys: 0.439 ± 0.025	2.134ProAsp: 2.134 ± 0.061	3.46ProGlu: 3.46 ± 0.071	1.661ProPhe: 1.661 ± 0.043	2.476ProGly: 2.476 ± 0.061	0.535ProHis: 0.535 ± 0.025	1.98ProIle: 1.98 ± 0.051	1.657ProLys: 1.657 ± 0.047	2.716ProLeu: 2.716 ± 0.062	0.907ProMet: 0.907 ± 0.032	0.988ProAsn: 0.988 ± 0.036	0.672ProPro: 0.672 ± 0.029	1.004ProGln: 1.004 ± 0.036	1.045ProArg: 1.045 ± 0.041	1.92ProSer: 1.92 ± 0.052	1.469ProThr: 1.469 ± 0.053	3.066ProVal: 3.066 ± 0.067	0.321ProTrp: 0.321 ± 0.022	1.671ProTyr: 1.671 ± 0.044	0.001ProXaa: 0.001 ± 0.001
Gln	2.679GlnAla: 2.679 ± 0.07	0.303GlnCys: 0.303 ± 0.018	1.731GlnAsp: 1.731 ± 0.051	3.372GlnGlu: 3.372 ± 0.077	1.164GlnPhe: 1.164 ± 0.035	2.217GlnGly: 2.217 ± 0.062	0.458GlnHis: 0.458 ± 0.027	2.872GlnIle: 2.872 ± 0.065	3.128GlnLys: 3.128 ± 0.075	2.765GlnLeu: 2.765 ± 0.07	1.224GlnMet: 1.224 ± 0.044	1.676GlnAsn: 1.676 ± 0.055	0.842GlnPro: 0.842 ± 0.039	1.301GlnGln: 1.301 ± 0.044	1.498GlnArg: 1.498 ± 0.044	1.666GlnSer: 1.666 ± 0.055	1.798GlnThr: 1.798 ± 0.047	2.486GlnVal: 2.486 ± 0.055	0.283GlnTrp: 0.283 ± 0.02	1.34GlnTyr: 1.34 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	2.768ArgAla: 2.768 ± 0.059	0.633ArgCys: 0.633 ± 0.029	2.163ArgAsp: 2.163 ± 0.055	4.427ArgGlu: 4.427 ± 0.102	1.885ArgPhe: 1.885 ± 0.05	2.598ArgGly: 2.598 ± 0.057	0.727ArgHis: 0.727 ± 0.033	3.735ArgIle: 3.735 ± 0.08	3.718ArgLys: 3.718 ± 0.075	4.099ArgLeu: 4.099 ± 0.078	1.507ArgMet: 1.507 ± 0.045	1.882ArgAsn: 1.882 ± 0.046	1.368ArgPro: 1.368 ± 0.044	1.936ArgGln: 1.936 ± 0.048	2.355ArgArg: 2.355 ± 0.066	2.294ArgSer: 2.294 ± 0.056	2.029ArgThr: 2.029 ± 0.048	2.694ArgVal: 2.694 ± 0.062	0.408ArgTrp: 0.408 ± 0.025	1.965ArgTyr: 1.965 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	4.206SerAla: 4.206 ± 0.078	0.896SerCys: 0.896 ± 0.041	3.051SerAsp: 3.051 ± 0.065	3.735SerGlu: 3.735 ± 0.069	2.918SerPhe: 2.918 ± 0.063	4.884SerGly: 4.884 ± 0.084	0.988SerHis: 0.988 ± 0.042	3.754SerIle: 3.754 ± 0.077	3.218SerLys: 3.218 ± 0.065	5.333SerLeu: 5.333 ± 0.085	1.881SerMet: 1.881 ± 0.051	1.945SerAsn: 1.945 ± 0.058	1.812SerPro: 1.812 ± 0.058	1.74SerGln: 1.74 ± 0.042	2.88SerArg: 2.88 ± 0.064	3.378SerSer: 3.378 ± 0.078	2.526SerThr: 2.526 ± 0.058	4.167SerVal: 4.167 ± 0.077	0.516SerTrp: 0.516 ± 0.026	2.721SerTyr: 2.721 ± 0.068	0.001SerXaa: 0.001 ± 0.001
Thr	4.424ThrAla: 4.424 ± 0.09	0.663ThrCys: 0.663 ± 0.03	3.003ThrAsp: 3.003 ± 0.071	3.959ThrGlu: 3.959 ± 0.083	2.081ThrPhe: 2.081 ± 0.044	4.651ThrGly: 4.651 ± 0.082	0.779ThrHis: 0.779 ± 0.031	3.754ThrIle: 3.754 ± 0.078	2.768ThrLys: 2.768 ± 0.079	4.737ThrLeu: 4.737 ± 0.098	1.386ThrMet: 1.386 ± 0.038	1.695ThrAsn: 1.695 ± 0.057	1.965ThrPro: 1.965 ± 0.057	1.56ThrGln: 1.56 ± 0.047	2.033ThrArg: 2.033 ± 0.054	2.597ThrSer: 2.597 ± 0.062	2.509ThrThr: 2.509 ± 0.059	4.069ThrVal: 4.069 ± 0.087	0.398ThrTrp: 0.398 ± 0.022	2.076ThrTyr: 2.076 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	4.702ValAla: 4.702 ± 0.082	1.246ValCys: 1.246 ± 0.034	3.589ValAsp: 3.589 ± 0.074	4.615ValGlu: 4.615 ± 0.084	3.092ValPhe: 3.092 ± 0.066	4.25ValGly: 4.25 ± 0.078	1.027ValHis: 1.027 ± 0.037	5.433ValIle: 5.433 ± 0.1	4.346ValLys: 4.346 ± 0.079	6.733ValLeu: 6.733 ± 0.101	2.129ValMet: 2.129 ± 0.052	2.775ValAsn: 2.775 ± 0.062	2.463ValPro: 2.463 ± 0.045	1.891ValGln: 1.891 ± 0.052	2.841ValArg: 2.841 ± 0.069	4.59ValSer: 4.59 ± 0.085	3.989ValThr: 3.989 ± 0.08	4.656ValVal: 4.656 ± 0.073	0.561ValTrp: 0.561 ± 0.032	2.724ValTyr: 2.724 ± 0.056	0.0ValXaa: 0.0 ± 0.0
Trp	0.497TrpAla: 0.497 ± 0.023	0.137TrpCys: 0.137 ± 0.013	0.503TrpAsp: 0.503 ± 0.027	0.622TrpGlu: 0.622 ± 0.028	0.435TrpPhe: 0.435 ± 0.021	0.692TrpGly: 0.692 ± 0.033	0.189TrpHis: 0.189 ± 0.016	0.683TrpIle: 0.683 ± 0.028	0.721TrpLys: 0.721 ± 0.026	0.872TrpLeu: 0.872 ± 0.041	0.311TrpMet: 0.311 ± 0.022	0.471TrpAsn: 0.471 ± 0.024	0.237TrpPro: 0.237 ± 0.02	0.396TrpGln: 0.396 ± 0.02	0.356TrpArg: 0.356 ± 0.023	0.448TrpSer: 0.448 ± 0.024	0.379TrpThr: 0.379 ± 0.025	0.489TrpVal: 0.489 ± 0.024	0.13TrpTrp: 0.13 ± 0.011	0.41TrpTyr: 0.41 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.838TyrAla: 2.838 ± 0.059	0.64TyrCys: 0.64 ± 0.029	2.619TyrAsp: 2.619 ± 0.055	3.524TyrGlu: 3.524 ± 0.068	1.988TyrPhe: 1.988 ± 0.044	3.33TyrGly: 3.33 ± 0.068	0.967TyrHis: 0.967 ± 0.037	2.593TyrIle: 2.593 ± 0.056	2.024TyrLys: 2.024 ± 0.059	4.451TyrLeu: 4.451 ± 0.092	1.183TyrMet: 1.183 ± 0.038	1.654TyrAsn: 1.654 ± 0.048	1.525TyrPro: 1.525 ± 0.044	1.79TyrGln: 1.79 ± 0.053	2.143TyrArg: 2.143 ± 0.057	2.28TyrSer: 2.28 ± 0.057	2.205TyrThr: 2.205 ± 0.054	2.812TyrVal: 2.812 ± 0.063	0.351TyrTrp: 0.351 ± 0.024	2.112TyrTyr: 2.112 ± 0.07	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.002	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.001XaaIle: 0.001 ± 0.001	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.003XaaArg: 0.003 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.016XaaXaa: 0.016 ± 0.006

Statistics based on 2499 proteins (794839 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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