Tupaia paramyxovirus (TPMV)
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9WS40|NCAP_TPMV Nucleoprotein OS=Tupaia paramyxovirus OX=92129 GN=N PE=3 SV=1
MM1 pKa = 7.91 ADD3 pKa = 4.19 LFSKK7 pKa = 11.11 VNDD10 pKa = 3.71 FQKK13 pKa = 10.86 YY14 pKa = 5.08 RR15 pKa = 11.84 TNLGRR20 pKa = 11.84 QGGLTVKK27 pKa = 10.5 LVGVRR32 pKa = 11.84 STVVVLVPSTKK43 pKa = 9.96 DD44 pKa = 2.9 HH45 pKa = 6.45 RR46 pKa = 11.84 LRR48 pKa = 11.84 WKK50 pKa = 10.66 LIRR53 pKa = 11.84 LLTLAVYY60 pKa = 10.55 NDD62 pKa = 4.24 SLPDD66 pKa = 4.1 SISIGALLSLLAISFEE82 pKa = 4.03 QPAAVIRR89 pKa = 11.84 GLLSDD94 pKa = 4.94 PDD96 pKa = 4.93 LEE98 pKa = 4.34 VQMIEE103 pKa = 5.1 VSLDD107 pKa = 3.31 DD108 pKa = 3.34 QGEE111 pKa = 3.97 IRR113 pKa = 11.84 FAARR117 pKa = 11.84 GDD119 pKa = 3.07 ILTRR123 pKa = 11.84 YY124 pKa = 9.1 KK125 pKa = 10.57 DD126 pKa = 3.34 AYY128 pKa = 9.96 FEE130 pKa = 4.75 KK131 pKa = 10.51 IRR133 pKa = 11.84 DD134 pKa = 4.01 FPNPDD139 pKa = 3.32 DD140 pKa = 4.77 DD141 pKa = 5.05 LAIFEE146 pKa = 4.77 DD147 pKa = 4.9 PEE149 pKa = 5.54 LGDD152 pKa = 4.02 YY153 pKa = 11.32 SDD155 pKa = 3.68 ITQDD159 pKa = 3.41 EE160 pKa = 4.64 YY161 pKa = 11.62 QAMITTITIQLWILLTKK178 pKa = 10.58 AVTAPDD184 pKa = 3.73 TAHH187 pKa = 7.47 DD188 pKa = 3.81 SEE190 pKa = 3.96 QRR192 pKa = 11.84 RR193 pKa = 11.84 FIKK196 pKa = 10.49 YY197 pKa = 8.08 LQQRR201 pKa = 11.84 KK202 pKa = 9.35 AYY204 pKa = 9.8 AAFKK208 pKa = 9.08 FTTIFTEE215 pKa = 4.14 RR216 pKa = 11.84 VRR218 pKa = 11.84 RR219 pKa = 11.84 KK220 pKa = 9.03 IAQSLSIRR228 pKa = 11.84 KK229 pKa = 9.04 FMVSIMLEE237 pKa = 4.04 VRR239 pKa = 11.84 KK240 pKa = 10.47 SGSAKK245 pKa = 10.18 GRR247 pKa = 11.84 ISEE250 pKa = 4.77 CIADD254 pKa = 3.43 VSAYY258 pKa = 9.62 IEE260 pKa = 4.06 EE261 pKa = 4.78 AGLSGFILTLKK272 pKa = 10.32 YY273 pKa = 10.77 GIGTRR278 pKa = 11.84 FPVLALNAFQSDD290 pKa = 3.5 LSVIRR295 pKa = 11.84 NLIDD299 pKa = 3.71 LYY301 pKa = 11.45 KK302 pKa = 11.02 SMGTIAPFMVLIEE315 pKa = 5.1 DD316 pKa = 3.8 ATQVKK321 pKa = 8.69 FAPGNYY327 pKa = 9.22 SLLWSFAMGVGTALDD342 pKa = 3.32 HH343 pKa = 7.49 AMNNLNINRR352 pKa = 11.84 DD353 pKa = 3.86 YY354 pKa = 11.57 LEE356 pKa = 4.02 PSYY359 pKa = 10.88 FRR361 pKa = 11.84 LGQEE365 pKa = 4.03 VVRR368 pKa = 11.84 LSEE371 pKa = 4.32 STVDD375 pKa = 3.21 RR376 pKa = 11.84 SMAQEE381 pKa = 4.22 LGIDD385 pKa = 3.79 PTSEE389 pKa = 4.0 DD390 pKa = 3.79 LIMRR394 pKa = 11.84 AVQAAGVGSRR404 pKa = 11.84 DD405 pKa = 2.95 PDD407 pKa = 3.33 AARR410 pKa = 11.84 RR411 pKa = 11.84 TGRR414 pKa = 11.84 FQVADD419 pKa = 3.52 IQIDD423 pKa = 3.72 EE424 pKa = 4.84 GPVDD428 pKa = 4.22 LATEE432 pKa = 4.75 AEE434 pKa = 4.26 DD435 pKa = 3.77 QTTKK439 pKa = 11.15 DD440 pKa = 2.97 NEE442 pKa = 4.02 QRR444 pKa = 11.84 IKK446 pKa = 11.19 VPDD449 pKa = 3.66 PRR451 pKa = 11.84 GSIGQSNAQFQPPKK465 pKa = 8.08 PQLRR469 pKa = 11.84 GRR471 pKa = 11.84 VMPPEE476 pKa = 4.63 RR477 pKa = 11.84 KK478 pKa = 8.42 PTDD481 pKa = 3.46 QQKK484 pKa = 10.15 NLQDD488 pKa = 3.39 QRR490 pKa = 11.84 PRR492 pKa = 11.84 PSATPRR498 pKa = 11.84 RR499 pKa = 11.84 LTKK502 pKa = 10.53 DD503 pKa = 2.92 AEE505 pKa = 4.49 DD506 pKa = 5.15 NIDD509 pKa = 3.87 QLFAQYY515 pKa = 10.87 DD516 pKa = 3.85 SGVAAPEE523 pKa = 4.09 DD524 pKa = 3.86 VTLVTSDD531 pKa = 3.24 STSPARR537 pKa = 11.84 SSGTGSDD544 pKa = 3.15 MDD546 pKa = 6.19 LINQSPP552 pKa = 3.25
Molecular weight: 61.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.122
IPC2_protein 5.118
IPC_protein 5.08
Toseland 4.94
ProMoST 5.207
Dawson 5.041
Bjellqvist 5.194
Wikipedia 4.94
Rodwell 4.927
Grimsley 4.851
Solomon 5.041
Lehninger 5.003
Nozaki 5.156
DTASelect 5.372
Thurlkill 4.94
EMBOSS 4.965
Sillero 5.207
Patrickios 4.482
IPC_peptide 5.054
IPC2_peptide 5.194
IPC2.peptide.svr19 5.276
Protein with the highest isoelectric point:
>sp|Q9QM81|V_TPMV Non-structural protein V OS=Tupaia paramyxovirus OX=92129 GN=P/V PE=3 SV=1
MM1 pKa = 7.13 YY2 pKa = 9.38 PSAPRR7 pKa = 11.84 IDD9 pKa = 3.82 EE10 pKa = 4.4 APVVDD15 pKa = 5.14 CEE17 pKa = 4.56 YY18 pKa = 11.14 EE19 pKa = 4.4 FIPTTWLEE27 pKa = 3.88 KK28 pKa = 10.7 GYY30 pKa = 10.47 LSAMKK35 pKa = 10.5 VEE37 pKa = 4.53 SDD39 pKa = 3.66 HH40 pKa = 6.38 NGKK43 pKa = 9.97 IIPSVRR49 pKa = 11.84 VVNPGWGEE57 pKa = 4.13 RR58 pKa = 11.84 KK59 pKa = 8.13 TSGYY63 pKa = 9.47 MYY65 pKa = 10.58 LIMHH69 pKa = 7.25 GIVEE73 pKa = 4.72 DD74 pKa = 4.01 VPKK77 pKa = 11.09 DD78 pKa = 3.05 GDD80 pKa = 3.58 TEE82 pKa = 3.95 QRR84 pKa = 11.84 YY85 pKa = 9.69 SGKK88 pKa = 8.96 TYY90 pKa = 10.51 AAFPLGVGKK99 pKa = 10.96 SNATPDD105 pKa = 4.32 DD106 pKa = 4.72 LLTSMNKK113 pKa = 9.83 LQITVRR119 pKa = 11.84 RR120 pKa = 11.84 TAGAGEE126 pKa = 4.21 RR127 pKa = 11.84 IVFGNNAPLGALFPWRR143 pKa = 11.84 RR144 pKa = 11.84 VLDD147 pKa = 4.58 FGAVFTAYY155 pKa = 9.32 KK156 pKa = 9.95 VCLSVEE162 pKa = 4.58 SISLFTPQRR171 pKa = 11.84 FRR173 pKa = 11.84 PLFLTVTLLTDD184 pKa = 3.28 NGLYY188 pKa = 10.15 KK189 pKa = 10.67 APSLFADD196 pKa = 5.05 FRR198 pKa = 11.84 ASKK201 pKa = 10.5 AVSFNLLARR210 pKa = 11.84 LTVNNKK216 pKa = 9.56 SGKK219 pKa = 9.93 DD220 pKa = 3.68 YY221 pKa = 11.06 LATAPASDD229 pKa = 3.68 TKK231 pKa = 10.87 QVVSFMVHH239 pKa = 5.6 IGNFVRR245 pKa = 11.84 KK246 pKa = 10.13 GGDD249 pKa = 3.19 VYY251 pKa = 11.67 SNSYY255 pKa = 9.47 CKK257 pKa = 10.49 KK258 pKa = 10.39 KK259 pKa = 9.74 IDD261 pKa = 4.57 RR262 pKa = 11.84 MDD264 pKa = 3.34 LQFALGAVGGLSFHH278 pKa = 6.86 IKK280 pKa = 10.3 INGKK284 pKa = 7.38 MSKK287 pKa = 8.46 TLMTQLGFHH296 pKa = 7.11 RR297 pKa = 11.84 NLCYY301 pKa = 10.62 SIMDD305 pKa = 4.33 INPDD309 pKa = 3.55 LNKK312 pKa = 10.56 KK313 pKa = 8.53 IWNSSCRR320 pKa = 11.84 ITSVAAILQPSVSKK334 pKa = 10.75 DD335 pKa = 3.11 FKK337 pKa = 10.37 IYY339 pKa = 10.54 HH340 pKa = 6.85 DD341 pKa = 3.96 VFIDD345 pKa = 3.53 NTGKK349 pKa = 10.5 IMGG352 pKa = 4.27
Molecular weight: 39.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.775
IPC2_protein 8.726
IPC_protein 8.624
Toseland 9.282
ProMoST 9.121
Dawson 9.589
Bjellqvist 9.355
Wikipedia 9.765
Rodwell 9.853
Grimsley 9.663
Solomon 9.633
Lehninger 9.589
Nozaki 9.458
DTASelect 9.297
Thurlkill 9.443
EMBOSS 9.736
Sillero 9.56
Patrickios 4.749
IPC_peptide 9.633
IPC2_peptide 7.995
IPC2.peptide.svr19 7.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
5354
153
2270
669.3
75.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.753 ± 0.671
1.886 ± 0.303
5.977 ± 0.394
5.454 ± 0.699
3.044 ± 0.492
5.902 ± 0.508
2.26 ± 0.234
6.911 ± 0.533
5.902 ± 0.344
10.105 ± 0.554
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.409 ± 0.234
5.118 ± 0.46
5.043 ± 0.404
4.053 ± 0.414
4.968 ± 0.485
8.797 ± 0.413
6.22 ± 0.392
4.968 ± 0.366
1.102 ± 0.164
4.128 ± 0.816
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here