Amino acid dipepetide frequency for Vaccinia virus (strain Tian Tan) (VACV)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.481AlaAla: 2.481 ± 0.234
1.029AlaCys: 1.029 ± 0.138
1.893AlaAsp: 1.893 ± 0.185
1.985AlaGlu: 1.985 ± 0.186
1.801AlaPhe: 1.801 ± 0.179
1.507AlaGly: 1.507 ± 0.16
0.496AlaHis: 0.496 ± 0.089
3.694AlaIle: 3.694 ± 0.264
2.812AlaLys: 2.812 ± 0.233
3.327AlaLeu: 3.327 ± 0.243
1.029AlaMet: 1.029 ± 0.121
2.371AlaAsn: 2.371 ± 0.201
1.103AlaPro: 1.103 ± 0.132
0.772AlaGln: 0.772 ± 0.107
1.691AlaArg: 1.691 ± 0.177
3.143AlaSer: 3.143 ± 0.24
2.297AlaThr: 2.297 ± 0.194
2.959AlaVal: 2.959 ± 0.233
0.276AlaTrp: 0.276 ± 0.064
1.581AlaTyr: 1.581 ± 0.178
0.0AlaXaa: 0.0 ± 0.0
Cys
0.827CysAla: 0.827 ± 0.119
0.57CysCys: 0.57 ± 0.103
1.323CysAsp: 1.323 ± 0.164
0.901CysGlu: 0.901 ± 0.15
0.827CysPhe: 0.827 ± 0.115
0.864CysGly: 0.864 ± 0.109
0.478CysHis: 0.478 ± 0.091
1.911CysIle: 1.911 ± 0.218
1.121CysLys: 1.121 ± 0.163
1.673CysLeu: 1.673 ± 0.16
0.662CysMet: 0.662 ± 0.1
1.25CysAsn: 1.25 ± 0.146
0.643CysPro: 0.643 ± 0.113
0.478CysGln: 0.478 ± 0.115
0.937CysArg: 0.937 ± 0.118
1.764CysSer: 1.764 ± 0.217
1.14CysThr: 1.14 ± 0.154
1.525CysVal: 1.525 ± 0.185
0.239CysTrp: 0.239 ± 0.071
1.084CysTyr: 1.084 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
2.702AspAla: 2.702 ± 0.204
1.011AspCys: 1.011 ± 0.134
4.926AspAsp: 4.926 ± 0.341
4.246AspGlu: 4.246 ± 0.245
2.941AspPhe: 2.941 ± 0.266
2.849AspGly: 2.849 ± 0.211
1.121AspHis: 1.121 ± 0.124
7.444AspIle: 7.444 ± 0.389
5.036AspLys: 5.036 ± 0.377
4.852AspLeu: 4.852 ± 0.28
1.728AspMet: 1.728 ± 0.157
4.356AspAsn: 4.356 ± 0.339
1.746AspPro: 1.746 ± 0.16
1.103AspGln: 1.103 ± 0.14
2.261AspArg: 2.261 ± 0.162
4.043AspSer: 4.043 ± 0.242
3.731AspThr: 3.731 ± 0.307
4.632AspVal: 4.632 ± 0.299
0.478AspTrp: 0.478 ± 0.098
3.253AspTyr: 3.253 ± 0.282
0.0AspXaa: 0.0 ± 0.0
Glu
2.15GluAla: 2.15 ± 0.166
1.011GluCys: 1.011 ± 0.155
3.455GluAsp: 3.455 ± 0.251
3.051GluGlu: 3.051 ± 0.25
2.408GluPhe: 2.408 ± 0.201
1.617GluGly: 1.617 ± 0.171
1.25GluHis: 1.25 ± 0.156
4.834GluIle: 4.834 ± 0.317
3.602GluLys: 3.602 ± 0.268
5.183GluLeu: 5.183 ± 0.356
1.562GluMet: 1.562 ± 0.172
3.382GluAsn: 3.382 ± 0.284
1.691GluPro: 1.691 ± 0.195
1.47GluGln: 1.47 ± 0.173
2.812GluArg: 2.812 ± 0.289
3.915GluSer: 3.915 ± 0.276
3.235GluThr: 3.235 ± 0.259
2.463GluVal: 2.463 ± 0.23
0.496GluTrp: 0.496 ± 0.095
3.639GluTyr: 3.639 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
1.599PheAla: 1.599 ± 0.175
0.956PheCys: 0.956 ± 0.136
3.216PheAsp: 3.216 ± 0.234
1.967PheGlu: 1.967 ± 0.182
2.702PhePhe: 2.702 ± 0.229
2.077PheGly: 2.077 ± 0.184
0.698PheHis: 0.698 ± 0.112
4.981PheIle: 4.981 ± 0.262
3.547PheLys: 3.547 ± 0.245
4.54PheLeu: 4.54 ± 0.336
1.47PheMet: 1.47 ± 0.152
3.529PheAsn: 3.529 ± 0.262
1.525PhePro: 1.525 ± 0.197
0.901PheGln: 0.901 ± 0.11
2.077PheArg: 2.077 ± 0.179
4.099PheSer: 4.099 ± 0.299
3.18PheThr: 3.18 ± 0.221
3.308PheVal: 3.308 ± 0.247
0.368PheTrp: 0.368 ± 0.091
2.297PheTyr: 2.297 ± 0.196
0.0PheXaa: 0.0 ± 0.0
Gly
1.728GlyAla: 1.728 ± 0.175
0.845GlyCys: 0.845 ± 0.12
2.61GlyAsp: 2.61 ± 0.193
2.058GlyGlu: 2.058 ± 0.187
1.838GlyPhe: 1.838 ± 0.176
2.114GlyGly: 2.114 ± 0.233
0.79GlyHis: 0.79 ± 0.122
4.08GlyIle: 4.08 ± 0.299
3.033GlyLys: 3.033 ± 0.209
3.033GlyLeu: 3.033 ± 0.254
0.937GlyMet: 0.937 ± 0.135
2.922GlyAsn: 2.922 ± 0.197
0.956GlyPro: 0.956 ± 0.139
0.607GlyGln: 0.607 ± 0.099
1.911GlyArg: 1.911 ± 0.175
2.941GlySer: 2.941 ± 0.233
2.169GlyThr: 2.169 ± 0.182
2.463GlyVal: 2.463 ± 0.2
0.276GlyTrp: 0.276 ± 0.067
2.058GlyTyr: 2.058 ± 0.215
0.0GlyXaa: 0.0 ± 0.0
His
0.809HisAla: 0.809 ± 0.108
0.478HisCys: 0.478 ± 0.11
1.14HisAsp: 1.14 ± 0.14
0.937HisGlu: 0.937 ± 0.13
0.901HisPhe: 0.901 ± 0.138
1.195HisGly: 1.195 ± 0.15
0.533HisHis: 0.533 ± 0.11
2.242HisIle: 2.242 ± 0.227
1.195HisLys: 1.195 ± 0.141
2.077HisLeu: 2.077 ± 0.161
0.625HisMet: 0.625 ± 0.111
1.084HisAsn: 1.084 ± 0.153
0.864HisPro: 0.864 ± 0.106
0.588HisGln: 0.588 ± 0.1
1.011HisArg: 1.011 ± 0.151
1.544HisSer: 1.544 ± 0.153
1.268HisThr: 1.268 ± 0.129
1.397HisVal: 1.397 ± 0.153
0.165HisTrp: 0.165 ± 0.056
0.809HisTyr: 0.809 ± 0.127
0.0HisXaa: 0.0 ± 0.0
Ile
3.069IleAla: 3.069 ± 0.2
1.746IleCys: 1.746 ± 0.165
6.929IleAsp: 6.929 ± 0.369
5.109IleGlu: 5.109 ± 0.335
4.668IlePhe: 4.668 ± 0.246
3.731IleGly: 3.731 ± 0.271
2.169IleHis: 2.169 ± 0.192
8.528IleIle: 8.528 ± 0.509
7.113IleLys: 7.113 ± 0.429
8.62IleLeu: 8.62 ± 0.4
2.297IleMet: 2.297 ± 0.194
7.719IleAsn: 7.719 ± 0.374
3.345IlePro: 3.345 ± 0.266
2.04IleGln: 2.04 ± 0.203
3.86IleArg: 3.86 ± 0.27
8.62IleSer: 8.62 ± 0.498
5.091IleThr: 5.091 ± 0.318
5.955IleVal: 5.955 ± 0.288
0.478IleTrp: 0.478 ± 0.092
4.209IleTyr: 4.209 ± 0.262
0.018IleXaa: 0.018 ± 0.018
Lys
2.058LysAla: 2.058 ± 0.191
1.617LysCys: 1.617 ± 0.154
4.411LysAsp: 4.411 ± 0.303
3.786LysGlu: 3.786 ± 0.307
3.106LysPhe: 3.106 ± 0.301
1.985LysGly: 1.985 ± 0.158
1.856LysHis: 1.856 ± 0.173
6.727LysIle: 6.727 ± 0.386
5.624LysLys: 5.624 ± 0.328
6.782LysLeu: 6.782 ± 0.307
2.022LysMet: 2.022 ± 0.212
4.889LysAsn: 4.889 ± 0.33
2.132LysPro: 2.132 ± 0.17
2.114LysGln: 2.114 ± 0.205
3.676LysArg: 3.676 ± 0.244
5.532LysSer: 5.532 ± 0.313
4.319LysThr: 4.319 ± 0.261
3.952LysVal: 3.952 ± 0.305
0.809LysTrp: 0.809 ± 0.155
4.356LysTyr: 4.356 ± 0.262
0.0LysXaa: 0.0 ± 0.0
Leu
3.566LeuAla: 3.566 ± 0.266
1.599LeuCys: 1.599 ± 0.192
5.845LeuAsp: 5.845 ± 0.349
5.165LeuGlu: 5.165 ± 0.298
5.109LeuPhe: 5.109 ± 0.313
3.088LeuGly: 3.088 ± 0.226
1.801LeuHis: 1.801 ± 0.214
7.37LeuIle: 7.37 ± 0.353
6.028LeuLys: 6.028 ± 0.373
9.52LeuLeu: 9.52 ± 0.54
2.72LeuMet: 2.72 ± 0.196
5.587LeuAsn: 5.587 ± 0.317
3.29LeuPro: 3.29 ± 0.201
1.893LeuGln: 1.893 ± 0.178
3.363LeuArg: 3.363 ± 0.269
8.344LeuSer: 8.344 ± 0.362
5.679LeuThr: 5.679 ± 0.316
5.716LeuVal: 5.716 ± 0.325
0.551LeuTrp: 0.551 ± 0.115
4.742LeuTyr: 4.742 ± 0.259
0.0LeuXaa: 0.0 ± 0.0
Met
1.525MetAla: 1.525 ± 0.167
0.533MetCys: 0.533 ± 0.085
2.058MetAsp: 2.058 ± 0.174
1.562MetGlu: 1.562 ± 0.182
1.525MetPhe: 1.525 ± 0.178
1.048MetGly: 1.048 ± 0.139
0.496MetHis: 0.496 ± 0.093
2.463MetIle: 2.463 ± 0.216
1.709MetLys: 1.709 ± 0.153
2.812MetLeu: 2.812 ± 0.238
0.992MetMet: 0.992 ± 0.109
1.82MetAsn: 1.82 ± 0.167
0.919MetPro: 0.919 ± 0.127
0.515MetGln: 0.515 ± 0.096
1.231MetArg: 1.231 ± 0.148
2.426MetSer: 2.426 ± 0.234
1.636MetThr: 1.636 ± 0.157
1.709MetVal: 1.709 ± 0.174
0.202MetTrp: 0.202 ± 0.06
1.507MetTyr: 1.507 ± 0.153
0.0MetXaa: 0.0 ± 0.0
Asn
2.849AsnAla: 2.849 ± 0.247
1.084AsnCys: 1.084 ± 0.156
4.282AsnAsp: 4.282 ± 0.316
3.584AsnGlu: 3.584 ± 0.288
2.794AsnPhe: 2.794 ± 0.204
3.198AsnGly: 3.198 ± 0.289
1.507AsnHis: 1.507 ± 0.166
7.407AsnIle: 7.407 ± 0.408
5.716AsnLys: 5.716 ± 0.311
5.036AsnLeu: 5.036 ± 0.326
2.132AsnMet: 2.132 ± 0.183
5.495AsnAsn: 5.495 ± 0.346
2.5AsnPro: 2.5 ± 0.223
1.25AsnGln: 1.25 ± 0.149
2.941AsnArg: 2.941 ± 0.225
4.852AsnSer: 4.852 ± 0.285
3.86AsnThr: 3.86 ± 0.249
4.338AsnVal: 4.338 ± 0.301
0.312AsnTrp: 0.312 ± 0.082
3.143AsnTyr: 3.143 ± 0.314
0.0AsnXaa: 0.0 ± 0.0
Pro
1.25ProAla: 1.25 ± 0.16
0.496ProCys: 0.496 ± 0.104
1.838ProAsp: 1.838 ± 0.185
2.426ProGlu: 2.426 ± 0.198
1.654ProPhe: 1.654 ± 0.183
1.305ProGly: 1.305 ± 0.16
0.698ProHis: 0.698 ± 0.132
3.069ProIle: 3.069 ± 0.24
2.15ProLys: 2.15 ± 0.19
3.014ProLeu: 3.014 ± 0.259
0.937ProMet: 0.937 ± 0.126
2.095ProAsn: 2.095 ± 0.172
1.415ProPro: 1.415 ± 0.175
0.754ProGln: 0.754 ± 0.126
1.507ProArg: 1.507 ± 0.198
2.794ProSer: 2.794 ± 0.234
2.132ProThr: 2.132 ± 0.19
1.967ProVal: 1.967 ± 0.166
0.257ProTrp: 0.257 ± 0.059
1.489ProTyr: 1.489 ± 0.179
0.0ProXaa: 0.0 ± 0.0
Gln
0.551GlnAla: 0.551 ± 0.108
0.496GlnCys: 0.496 ± 0.09
1.305GlnAsp: 1.305 ± 0.143
1.158GlnGlu: 1.158 ± 0.153
0.937GlnPhe: 0.937 ± 0.139
0.643GlnGly: 0.643 ± 0.125
0.625GlnHis: 0.625 ± 0.106
1.691GlnIle: 1.691 ± 0.166
1.268GlnLys: 1.268 ± 0.155
2.481GlnLeu: 2.481 ± 0.191
0.754GlnMet: 0.754 ± 0.114
1.36GlnAsn: 1.36 ± 0.142
0.625GlnPro: 0.625 ± 0.137
0.754GlnGln: 0.754 ± 0.127
1.305GlnArg: 1.305 ± 0.151
1.636GlnSer: 1.636 ± 0.161
1.47GlnThr: 1.47 ± 0.159
1.011GlnVal: 1.011 ± 0.121
0.257GlnTrp: 0.257 ± 0.065
1.581GlnTyr: 1.581 ± 0.153
0.0GlnXaa: 0.0 ± 0.0
Arg
1.213ArgAla: 1.213 ± 0.135
1.048ArgCys: 1.048 ± 0.142
2.72ArgAsp: 2.72 ± 0.221
2.297ArgGlu: 2.297 ± 0.305
2.261ArgPhe: 2.261 ± 0.21
1.801ArgGly: 1.801 ± 0.219
1.342ArgHis: 1.342 ± 0.176
3.474ArgIle: 3.474 ± 0.206
2.481ArgLys: 2.481 ± 0.223
4.356ArgLeu: 4.356 ± 0.278
1.195ArgMet: 1.195 ± 0.156
2.904ArgAsn: 2.904 ± 0.233
1.305ArgPro: 1.305 ± 0.157
1.36ArgGln: 1.36 ± 0.165
2.775ArgArg: 2.775 ± 0.261
3.382ArgSer: 3.382 ± 0.26
2.187ArgThr: 2.187 ± 0.183
2.353ArgVal: 2.353 ± 0.194
0.441ArgTrp: 0.441 ± 0.092
2.628ArgTyr: 2.628 ± 0.25
0.0ArgXaa: 0.0 ± 0.0
Ser
3.014SerAla: 3.014 ± 0.231
1.562SerCys: 1.562 ± 0.184
4.668SerAsp: 4.668 ± 0.317
3.602SerGlu: 3.602 ± 0.263
4.227SerPhe: 4.227 ± 0.328
3.566SerGly: 3.566 ± 0.247
1.489SerHis: 1.489 ± 0.17
7.811SerIle: 7.811 ± 0.418
6.047SerLys: 6.047 ± 0.343
7.37SerLeu: 7.37 ± 0.379
2.536SerMet: 2.536 ± 0.222
5.073SerAsn: 5.073 ± 0.336
3.327SerPro: 3.327 ± 0.293
1.93SerGln: 1.93 ± 0.194
3.106SerArg: 3.106 ± 0.291
7.26SerSer: 7.26 ± 0.499
5.477SerThr: 5.477 ± 0.389
4.815SerVal: 4.815 ± 0.258
0.386SerTrp: 0.386 ± 0.088
3.547SerTyr: 3.547 ± 0.248
0.0SerXaa: 0.0 ± 0.0
Thr
2.279ThrAla: 2.279 ± 0.189
1.268ThrCys: 1.268 ± 0.167
4.117ThrAsp: 4.117 ± 0.299
3.327ThrGlu: 3.327 ± 0.276
2.812ThrPhe: 2.812 ± 0.229
2.389ThrGly: 2.389 ± 0.207
1.213ThrHis: 1.213 ± 0.146
6.231ThrIle: 6.231 ± 0.351
4.264ThrLys: 4.264 ± 0.244
5.404ThrLeu: 5.404 ± 0.295
1.746ThrMet: 1.746 ± 0.186
3.676ThrAsn: 3.676 ± 0.264
2.187ThrPro: 2.187 ± 0.195
0.901ThrGln: 0.901 ± 0.12
2.334ThrArg: 2.334 ± 0.182
4.889ThrSer: 4.889 ± 0.282
3.86ThrThr: 3.86 ± 0.298
4.007ThrVal: 4.007 ± 0.269
0.496ThrTrp: 0.496 ± 0.093
2.702ThrTyr: 2.702 ± 0.216
0.0ThrXaa: 0.0 ± 0.0
Val
2.316ValAla: 2.316 ± 0.179
1.415ValCys: 1.415 ± 0.158
4.485ValAsp: 4.485 ± 0.28
3.566ValGlu: 3.566 ± 0.287
3.308ValPhe: 3.308 ± 0.267
1.801ValGly: 1.801 ± 0.196
1.158ValHis: 1.158 ± 0.136
5.642ValIle: 5.642 ± 0.326
4.76ValLys: 4.76 ± 0.315
5.091ValLeu: 5.091 ± 0.315
1.562ValMet: 1.562 ± 0.141
4.301ValAsn: 4.301 ± 0.243
1.911ValPro: 1.911 ± 0.173
1.25ValGln: 1.25 ± 0.15
2.591ValArg: 2.591 ± 0.2
5.165ValSer: 5.165 ± 0.333
4.007ValThr: 4.007 ± 0.252
3.694ValVal: 3.694 ± 0.309
0.368ValTrp: 0.368 ± 0.075
3.308ValTyr: 3.308 ± 0.245
0.0ValXaa: 0.0 ± 0.0
Trp
0.165TrpAla: 0.165 ± 0.064
0.239TrpCys: 0.239 ± 0.063
0.257TrpAsp: 0.257 ± 0.059
0.404TrpGlu: 0.404 ± 0.09
0.57TrpPhe: 0.57 ± 0.106
0.294TrpGly: 0.294 ± 0.072
0.11TrpHis: 0.11 ± 0.042
0.588TrpIle: 0.588 ± 0.135
0.662TrpLys: 0.662 ± 0.104
0.809TrpLeu: 0.809 ± 0.131
0.478TrpMet: 0.478 ± 0.107
0.441TrpAsn: 0.441 ± 0.1
0.202TrpPro: 0.202 ± 0.06
0.11TrpGln: 0.11 ± 0.044
0.221TrpArg: 0.221 ± 0.07
0.533TrpSer: 0.533 ± 0.101
0.404TrpThr: 0.404 ± 0.081
0.404TrpVal: 0.404 ± 0.088
0.018TrpTrp: 0.018 ± 0.022
0.294TrpTyr: 0.294 ± 0.071
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.095TyrAla: 2.095 ± 0.19
1.213TyrCys: 1.213 ± 0.166
2.977TyrAsp: 2.977 ± 0.259
2.095TyrGlu: 2.095 ± 0.22
2.647TyrPhe: 2.647 ± 0.199
2.297TyrGly: 2.297 ± 0.203
1.029TyrHis: 1.029 ± 0.132
5.109TyrIle: 5.109 ± 0.247
3.602TyrLys: 3.602 ± 0.217
4.999TyrLeu: 4.999 ± 0.348
1.287TyrMet: 1.287 ± 0.136
4.025TyrAsn: 4.025 ± 0.387
1.562TyrPro: 1.562 ± 0.167
1.121TyrGln: 1.121 ± 0.155
1.911TyrArg: 1.911 ± 0.161
3.933TyrSer: 3.933 ± 0.235
2.959TyrThr: 2.959 ± 0.201
3.033TyrVal: 3.033 ± 0.223
0.331TyrTrp: 0.331 ± 0.08
2.83TyrTyr: 2.83 ± 0.256
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.018XaaIle: 0.018 ± 0.018
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 239 proteins (54410 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski