Haloactinobacterium album
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4192 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H5FA42|A0A1H5FA42_9MICO PucR C-terminal helix-turn-helix domain-containing protein OS=Haloactinobacterium album OX=648782 GN=SAMN04488554_1279 PE=3 SV=1
MM1 pKa = 7.38 SRR3 pKa = 11.84 RR4 pKa = 11.84 ARR6 pKa = 11.84 WRR8 pKa = 11.84 RR9 pKa = 11.84 AAGVFTVALLVATGLAAVEE28 pKa = 4.23 ATVAPAPAASAASSPDD44 pKa = 2.94 GTGIRR49 pKa = 11.84 QSQTFYY55 pKa = 10.97 AYY57 pKa = 10.28 ADD59 pKa = 3.5 AGEE62 pKa = 4.37 TVSAAFEE69 pKa = 4.44 VVNRR73 pKa = 11.84 WSFTGSAVFTVTAPDD88 pKa = 4.34 GATQTCTTPAGAALGSVCEE107 pKa = 4.22 IGPMTSEE114 pKa = 4.1 TSGVWTVDD122 pKa = 2.37 VHH124 pKa = 7.59 RR125 pKa = 11.84 PGDD128 pKa = 3.5 FGGAFTWDD136 pKa = 2.92 ITVADD141 pKa = 4.94 GGTAIPGRR149 pKa = 11.84 VWTDD153 pKa = 2.88 RR154 pKa = 11.84 FLMWNRR160 pKa = 11.84 EE161 pKa = 4.06 FVDD164 pKa = 3.53 LTLWYY169 pKa = 10.17 VGASGHH175 pKa = 5.34 QYY177 pKa = 9.04 EE178 pKa = 4.45 VSYY181 pKa = 11.17 FDD183 pKa = 3.96 FYY185 pKa = 11.39 GIEE188 pKa = 4.3 SLFSSNQFGVVYY200 pKa = 10.7 DD201 pKa = 4.24 GTCEE205 pKa = 3.76 SAYY208 pKa = 10.52 QSAEE212 pKa = 3.88 DD213 pKa = 4.36 RR214 pKa = 11.84 PSGPYY219 pKa = 10.13 YY220 pKa = 10.15 DD221 pKa = 5.38 DD222 pKa = 4.44 HH223 pKa = 8.22 LSNSGQEE230 pKa = 4.33 CGPLYY235 pKa = 11.36 NMFFAPPAADD245 pKa = 4.22 LPASAADD252 pKa = 3.52 ATGATQFILPPVQPPTVSEE271 pKa = 4.12 LRR273 pKa = 11.84 FTPDD277 pKa = 2.79 TPDD280 pKa = 3.09 TRR282 pKa = 11.84 EE283 pKa = 3.94 GTFSFEE289 pKa = 4.2 TNHH292 pKa = 6.12 VGNADD297 pKa = 4.1 LLIDD301 pKa = 4.02 ADD303 pKa = 4.5 GNGSYY308 pKa = 11.03 DD309 pKa = 3.55 DD310 pKa = 4.02 TVDD313 pKa = 2.98 RR314 pKa = 11.84 TIPVSVNTGAEE325 pKa = 3.82 PSVAFDD331 pKa = 3.79 GLDD334 pKa = 3.59 GEE336 pKa = 5.33 GNAISPSTRR345 pKa = 11.84 FNATMRR351 pKa = 11.84 ITQAGEE357 pKa = 3.45 IHH359 pKa = 6.4 FVNGDD364 pKa = 3.3 VEE366 pKa = 4.65 SRR368 pKa = 11.84 GGISVTALTGTADD381 pKa = 3.88 GTSTLYY387 pKa = 10.57 WNDD390 pKa = 3.53 SEE392 pKa = 4.99 LQTEE396 pKa = 4.54 EE397 pKa = 5.13 RR398 pKa = 11.84 DD399 pKa = 3.91 QSCLPPVLDD408 pKa = 4.73 GRR410 pKa = 11.84 DD411 pKa = 3.9 GQDD414 pKa = 2.83 SSVSGGVHH422 pKa = 6.54 GWPCTPRR429 pKa = 11.84 NPNDD433 pKa = 3.56 GVTGPWGDD441 pKa = 3.43 VRR443 pKa = 11.84 LIDD446 pKa = 4.27 DD447 pKa = 3.61 WTFNPVDD454 pKa = 3.52 VSASVEE460 pKa = 3.87 IPAAEE465 pKa = 4.06 GNYY468 pKa = 9.68 VVTKK472 pKa = 10.75 SSDD475 pKa = 3.82 PEE477 pKa = 4.68 PGTAVHH483 pKa = 6.77 PGDD486 pKa = 3.46 VVTYY490 pKa = 8.45 TIEE493 pKa = 3.9 VAQIGNDD500 pKa = 3.19 SAAANVSDD508 pKa = 5.1 DD509 pKa = 4.41 LSDD512 pKa = 3.9 VLDD515 pKa = 4.32 DD516 pKa = 4.41 ASYY519 pKa = 11.51 NGDD522 pKa = 3.28 ASATAGEE529 pKa = 4.23 VDD531 pKa = 3.74 VTGTTLNWNGTLLDD545 pKa = 4.29 GGSARR550 pKa = 11.84 ITYY553 pKa = 10.29 SVTVDD558 pKa = 3.45 DD559 pKa = 5.57 AGDD562 pKa = 3.46 VSSGDD567 pKa = 3.56 GQVGNVVTSPGCGSDD582 pKa = 3.21 ACATEE587 pKa = 4.73 HH588 pKa = 6.74 PVGDD592 pKa = 3.74 YY593 pKa = 10.8 SVEE596 pKa = 4.04 KK597 pKa = 10.39 SAEE600 pKa = 4.03 PAPGSAVTDD609 pKa = 4.03 GDD611 pKa = 4.09 TVAYY615 pKa = 8.03 TLTVAQEE622 pKa = 3.68 GAAAVEE628 pKa = 4.62 GASLVDD634 pKa = 4.34 DD635 pKa = 5.02 LSDD638 pKa = 3.78 VLDD641 pKa = 4.24 DD642 pKa = 4.68 AVWNGDD648 pKa = 3.48 LVASAGAAEE657 pKa = 4.25 FDD659 pKa = 3.85 EE660 pKa = 5.23 DD661 pKa = 3.85 AQEE664 pKa = 4.31 LRR666 pKa = 11.84 WSGDD670 pKa = 3.18 LGIDD674 pKa = 3.51 DD675 pKa = 4.95 VVTISYY681 pKa = 10.85 SVMVTGSGDD690 pKa = 3.38 TSVDD694 pKa = 3.12 NTVASDD700 pKa = 3.84 GCGTAEE706 pKa = 4.31 EE707 pKa = 4.92 CQSSHH712 pKa = 7.19 PYY714 pKa = 9.86 GGYY717 pKa = 10.55 GVVKK721 pKa = 10.36 SADD724 pKa = 3.64 PAPGGGVAEE733 pKa = 4.82 GEE735 pKa = 4.55 TVAYY739 pKa = 8.26 TLTVTQSGQGSVPAEE754 pKa = 4.02 LSDD757 pKa = 5.13 DD758 pKa = 3.73 LTAVLDD764 pKa = 4.0 DD765 pKa = 4.49 AVYY768 pKa = 11.19 NDD770 pKa = 5.08 DD771 pKa = 4.17 LQASAGAASYY781 pKa = 10.79 DD782 pKa = 4.13 SEE784 pKa = 5.13 SGTIDD789 pKa = 2.87 WDD791 pKa = 3.63 GTLARR796 pKa = 11.84 GDD798 pKa = 3.88 VVTISYY804 pKa = 10.54 SVTVQPVGTGDD815 pKa = 3.16 GSLRR819 pKa = 11.84 NVVTSPGCDD828 pKa = 2.96 SDD830 pKa = 4.36 ADD832 pKa = 3.92 CVTEE836 pKa = 3.79 NRR838 pKa = 11.84 VGGFVYY844 pKa = 10.52 SKK846 pKa = 10.69 SADD849 pKa = 3.58 GGPGSQVSQGDD860 pKa = 3.98 VITYY864 pKa = 10.08 SVTVTHH870 pKa = 6.93 TGAGPVAEE878 pKa = 4.76 ARR880 pKa = 11.84 VSDD883 pKa = 4.33 DD884 pKa = 3.64 LSGVLDD890 pKa = 4.4 DD891 pKa = 5.26 ATFNDD896 pKa = 4.46 DD897 pKa = 3.65 ASASSGSIRR906 pKa = 11.84 VDD908 pKa = 3.0 GSTLDD913 pKa = 3.49 WTGSLAPGDD922 pKa = 3.96 VVEE925 pKa = 4.51 ITYY928 pKa = 10.86 SVTVTGAGDD937 pKa = 3.6 LAVDD941 pKa = 3.83 NVVLTDD947 pKa = 4.15 DD948 pKa = 4.16 PRR950 pKa = 11.84 GVCDD954 pKa = 4.75 PDD956 pKa = 3.45 ATCATEE962 pKa = 4.26 HH963 pKa = 5.74 QVPPSPDD970 pKa = 3.24 LPATGARR977 pKa = 11.84 VGAWVIPAALLMTGLGALVLMGARR1001 pKa = 11.84 RR1002 pKa = 11.84 HH1003 pKa = 5.5 RR1004 pKa = 11.84 AAEE1007 pKa = 4.02 KK1008 pKa = 10.58
Molecular weight: 103.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.676
IPC2_protein 3.668
IPC_protein 3.719
Toseland 3.49
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.541
EMBOSS 3.681
Sillero 3.846
Patrickios 1.214
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A1H5LNV2|A0A1H5LNV2_9MICO TIR domain-containing protein OS=Haloactinobacterium album OX=648782 GN=SAMN04488554_2902 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.45 VHH17 pKa = 5.31 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 SILAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.97 GRR40 pKa = 11.84 TEE42 pKa = 3.99 LSAA45 pKa = 4.86
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.453
IPC2_protein 10.979
IPC_protein 12.574
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.735
Wikipedia 13.217
Rodwell 12.34
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.735
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.076
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4192
0
4192
1448774
29
2441
345.6
37.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.017 ± 0.05
0.611 ± 0.009
6.407 ± 0.043
5.96 ± 0.036
2.844 ± 0.022
9.119 ± 0.042
2.261 ± 0.021
3.902 ± 0.026
1.314 ± 0.022
10.074 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.895 ± 0.016
1.876 ± 0.019
5.764 ± 0.03
2.994 ± 0.017
7.247 ± 0.052
5.488 ± 0.027
6.569 ± 0.037
8.957 ± 0.038
1.645 ± 0.019
2.057 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here