Citrobacter phage vB_CfrM_CfP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Pseudotevenvirus; unclassified Pseudotevenvirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 272 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1IXL5|A0A1B1IXL5_9CAUD Uncharacterized protein OS=Citrobacter phage vB_CfrM_CfP1 OX=1871313 GN=ABCD_0139 PE=4 SV=1
MM1 pKa = 7.78NNFDD5 pKa = 3.66WGNAEE10 pKa = 3.98MQAIPDD16 pKa = 3.63AEE18 pKa = 4.14EE19 pKa = 3.86PVFDD23 pKa = 4.88SPHH26 pKa = 5.52SSKK29 pKa = 10.45WIVAIDD35 pKa = 3.56DD36 pKa = 3.75EE37 pKa = 5.76GYY39 pKa = 8.62VTVLSRR45 pKa = 11.84PNIHH49 pKa = 7.63DD50 pKa = 3.6SFFEE54 pKa = 4.22CGRR57 pKa = 11.84SAEE60 pKa = 4.86DD61 pKa = 2.84IGLPDD66 pKa = 4.4SVDD69 pKa = 3.63MPPGVYY75 pKa = 9.6EE76 pKa = 4.3WICGFDD82 pKa = 3.26QTTDD86 pKa = 2.87WEE88 pKa = 4.36TGYY91 pKa = 11.05VDD93 pKa = 3.11GWEE96 pKa = 4.47FYY98 pKa = 10.33PNEE101 pKa = 4.04STLLYY106 pKa = 10.34SWDD109 pKa = 3.37NGEE112 pKa = 4.25VNKK115 pKa = 10.8SGNEE119 pKa = 3.8EE120 pKa = 4.11TQSDD124 pKa = 3.95NGSGGVV130 pKa = 3.25

Molecular weight: 14.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1IXV8|A0A1B1IXV8_9CAUD Uncharacterized protein OS=Citrobacter phage vB_CfrM_CfP1 OX=1871313 GN=ABCD_0112 PE=4 SV=1
MM1 pKa = 7.93RR2 pKa = 11.84IAVLGGTMISRR13 pKa = 11.84TLVSCLIDD21 pKa = 3.53SIRR24 pKa = 11.84WVNTPCVDD32 pKa = 3.44LCSRR36 pKa = 11.84IPYY39 pKa = 9.52EE40 pKa = 4.03EE41 pKa = 3.94PTPYY45 pKa = 9.97KK46 pKa = 10.14VKK48 pKa = 10.04PYY50 pKa = 9.6KK51 pKa = 10.42APRR54 pKa = 11.84INRR57 pKa = 11.84ALHH60 pKa = 5.1RR61 pKa = 11.84QSVNRR66 pKa = 11.84GKK68 pKa = 10.66

Molecular weight: 7.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

272

0

272

56347

37

1219

207.2

23.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.994 ± 0.19

1.159 ± 0.069

6.675 ± 0.1

6.886 ± 0.172

4.146 ± 0.112

6.236 ± 0.185

1.8 ± 0.077

6.591 ± 0.121

7.292 ± 0.19

7.663 ± 0.131

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.925 ± 0.098

5.466 ± 0.134

3.615 ± 0.068

3.278 ± 0.099

4.806 ± 0.107

5.775 ± 0.124

5.956 ± 0.226

7.067 ± 0.136

1.342 ± 0.052

4.329 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski