Papaya leaf curl betasatellite
Average proteome isoelectric point is 4.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q80MT8|Q80MT8_9VIRU C4 protein OS=Papaya leaf curl betasatellite OX=714640 GN=C4 PE=4 SV=1
MM1 pKa = 7.12 TIKK4 pKa = 10.57 YY5 pKa = 9.37 VNQKK9 pKa = 10.6 GLVFVINVTLRR20 pKa = 11.84 GDD22 pKa = 3.55 EE23 pKa = 4.3 SIKK26 pKa = 10.91 VYY28 pKa = 10.42 IQLTSTRR35 pKa = 11.84 SPALVKK41 pKa = 10.5 KK42 pKa = 10.62 KK43 pKa = 10.51 FMLPYY48 pKa = 9.24 KK49 pKa = 9.72 HH50 pKa = 7.09 DD51 pKa = 4.7 GIIPPFDD58 pKa = 3.94 FNNLEE63 pKa = 3.87 EE64 pKa = 4.87 GIRR67 pKa = 11.84 NILAIMYY74 pKa = 8.94 RR75 pKa = 11.84 DD76 pKa = 3.33 SSFDD80 pKa = 3.25 EE81 pKa = 4.62 FRR83 pKa = 11.84 QEE85 pKa = 5.21 DD86 pKa = 3.77 MTEE89 pKa = 4.18 IIDD92 pKa = 3.54 ILMMHH97 pKa = 7.11 EE98 pKa = 4.6 APVFDD103 pKa = 3.28 IQIFDD108 pKa = 3.78 EE109 pKa = 4.99 YY110 pKa = 11.17 GVCTNVSAA118 pKa = 5.1
Molecular weight: 13.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.872
IPC2_protein 4.952
IPC_protein 4.8
Toseland 4.673
ProMoST 4.927
Dawson 4.774
Bjellqvist 4.914
Wikipedia 4.66
Rodwell 4.66
Grimsley 4.596
Solomon 4.762
Lehninger 4.724
Nozaki 4.889
DTASelect 5.054
Thurlkill 4.685
EMBOSS 4.673
Sillero 4.94
Patrickios 3.999
IPC_peptide 4.774
IPC2_peptide 4.927
IPC2.peptide.svr19 4.946
Protein with the highest isoelectric point:
>tr|Q80MT8|Q80MT8_9VIRU C4 protein OS=Papaya leaf curl betasatellite OX=714640 GN=C4 PE=4 SV=1
MM1 pKa = 7.12 TIKK4 pKa = 10.57 YY5 pKa = 9.37 VNQKK9 pKa = 10.6 GLVFVINVTLRR20 pKa = 11.84 GDD22 pKa = 3.55 EE23 pKa = 4.3 SIKK26 pKa = 10.91 VYY28 pKa = 10.42 IQLTSTRR35 pKa = 11.84 SPALVKK41 pKa = 10.5 KK42 pKa = 10.62 KK43 pKa = 10.51 FMLPYY48 pKa = 9.24 KK49 pKa = 9.72 HH50 pKa = 7.09 DD51 pKa = 4.7 GIIPPFDD58 pKa = 3.94 FNNLEE63 pKa = 3.87 EE64 pKa = 4.87 GIRR67 pKa = 11.84 NILAIMYY74 pKa = 8.94 RR75 pKa = 11.84 DD76 pKa = 3.33 SSFDD80 pKa = 3.25 EE81 pKa = 4.62 FRR83 pKa = 11.84 QEE85 pKa = 5.21 DD86 pKa = 3.77 MTEE89 pKa = 4.18 IIDD92 pKa = 3.54 ILMMHH97 pKa = 7.11 EE98 pKa = 4.6 APVFDD103 pKa = 3.28 IQIFDD108 pKa = 3.78 EE109 pKa = 4.99 YY110 pKa = 11.17 GVCTNVSAA118 pKa = 5.1
Molecular weight: 13.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.872
IPC2_protein 4.952
IPC_protein 4.8
Toseland 4.673
ProMoST 4.927
Dawson 4.774
Bjellqvist 4.914
Wikipedia 4.66
Rodwell 4.66
Grimsley 4.596
Solomon 4.762
Lehninger 4.724
Nozaki 4.889
DTASelect 5.054
Thurlkill 4.685
EMBOSS 4.673
Sillero 4.94
Patrickios 3.999
IPC_peptide 4.774
IPC2_peptide 4.927
IPC2.peptide.svr19 4.946
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
118
118
118
118.0
13.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.39 ± 0.0
0.847 ± 0.0
7.627 ± 0.0
6.78 ± 0.0
6.78 ± 0.0
4.237 ± 0.0
1.695 ± 0.0
11.864 ± 0.0
5.932 ± 0.0
6.78 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
5.085 ± 0.0
5.085 ± 0.0
4.237 ± 0.0
3.39 ± 0.0
4.237 ± 0.0
5.085 ± 0.0
5.085 ± 0.0
7.627 ± 0.0
0.0 ± 0.0
4.237 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here