Amino acid dipepetide frequency for Heliothis virescens (Tobacco budworm moth)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.365AlaAla: 8.365 ± 0.094	1.59AlaCys: 1.59 ± 0.049	3.63AlaAsp: 3.63 ± 0.032	4.556AlaGlu: 4.556 ± 0.035	2.175AlaPhe: 2.175 ± 0.023	4.58AlaGly: 4.58 ± 0.058	1.963AlaHis: 1.963 ± 0.033	3.253AlaIle: 3.253 ± 0.027	3.777AlaLys: 3.777 ± 0.034	7.188AlaLeu: 7.188 ± 0.068	1.558AlaMet: 1.558 ± 0.017	2.631AlaAsn: 2.631 ± 0.029	4.866AlaPro: 4.866 ± 0.073	2.791AlaGln: 2.791 ± 0.029	4.755AlaArg: 4.755 ± 0.062	5.208AlaSer: 5.208 ± 0.042	4.109AlaThr: 4.109 ± 0.043	4.882AlaVal: 4.882 ± 0.047	0.79AlaTrp: 0.79 ± 0.018	1.864AlaTyr: 1.864 ± 0.025	0.004AlaXaa: 0.004 ± 0.001
Cys	1.6CysAla: 1.6 ± 0.034	0.531CysCys: 0.531 ± 0.018	1.199CysAsp: 1.199 ± 0.02	1.223CysGlu: 1.223 ± 0.026	0.744CysPhe: 0.744 ± 0.021	1.557CysGly: 1.557 ± 0.051	0.505CysHis: 0.505 ± 0.013	1.027CysIle: 1.027 ± 0.029	1.085CysLys: 1.085 ± 0.024	1.747CysLeu: 1.747 ± 0.031	0.4CysMet: 0.4 ± 0.008	0.877CysAsn: 0.877 ± 0.017	1.272CysPro: 1.272 ± 0.056	0.753CysGln: 0.753 ± 0.023	1.346CysArg: 1.346 ± 0.046	1.665CysSer: 1.665 ± 0.044	1.213CysThr: 1.213 ± 0.033	1.509CysVal: 1.509 ± 0.04	0.244CysTrp: 0.244 ± 0.008	0.662CysTyr: 0.662 ± 0.027	0.001CysXaa: 0.001 ± 0.0
Asp	3.739AspAla: 3.739 ± 0.034	1.074AspCys: 1.074 ± 0.025	4.12AspAsp: 4.12 ± 0.054	4.405AspGlu: 4.405 ± 0.087	2.041AspPhe: 2.041 ± 0.023	3.254AspGly: 3.254 ± 0.026	1.167AspHis: 1.167 ± 0.014	3.278AspIle: 3.278 ± 0.025	3.646AspLys: 3.646 ± 0.067	4.564AspLeu: 4.564 ± 0.031	1.315AspMet: 1.315 ± 0.014	2.724AspAsn: 2.724 ± 0.035	2.714AspPro: 2.714 ± 0.053	1.667AspGln: 1.667 ± 0.017	2.72AspArg: 2.72 ± 0.036	4.273AspSer: 4.273 ± 0.04	3.294AspThr: 3.294 ± 0.038	3.772AspVal: 3.772 ± 0.028	0.615AspTrp: 0.615 ± 0.013	1.844AspTyr: 1.844 ± 0.023	0.001AspXaa: 0.001 ± 0.0
Glu	4.438GluAla: 4.438 ± 0.039	1.354GluCys: 1.354 ± 0.052	4.014GluAsp: 4.014 ± 0.039	5.761GluGlu: 5.761 ± 0.068	1.981GluPhe: 1.981 ± 0.019	3.154GluGly: 3.154 ± 0.036	1.579GluHis: 1.579 ± 0.024	3.489GluIle: 3.489 ± 0.033	5.023GluLys: 5.023 ± 0.109	5.73GluLeu: 5.73 ± 0.042	1.54GluMet: 1.54 ± 0.017	3.334GluAsn: 3.334 ± 0.03	3.382GluPro: 3.382 ± 0.042	2.62GluGln: 2.62 ± 0.031	4.116GluArg: 4.116 ± 0.055	4.343GluSer: 4.343 ± 0.042	3.638GluThr: 3.638 ± 0.038	4.008GluVal: 4.008 ± 0.037	0.686GluTrp: 0.686 ± 0.016	1.988GluTyr: 1.988 ± 0.026	0.003GluXaa: 0.003 ± 0.001
Phe	2.128PheAla: 2.128 ± 0.023	0.718PheCys: 0.718 ± 0.014	1.96PheAsp: 1.96 ± 0.021	1.979PheGlu: 1.979 ± 0.024	1.262PhePhe: 1.262 ± 0.017	2.148PheGly: 2.148 ± 0.026	0.816PheHis: 0.816 ± 0.014	1.869PheIle: 1.869 ± 0.02	1.949PheLys: 1.949 ± 0.019	2.973PheLeu: 2.973 ± 0.031	0.792PheMet: 0.792 ± 0.011	1.66PheAsn: 1.66 ± 0.018	1.55PhePro: 1.55 ± 0.026	1.234PheGln: 1.234 ± 0.017	1.727PheArg: 1.727 ± 0.017	2.529PheSer: 2.529 ± 0.025	1.974PheThr: 1.974 ± 0.021	2.242PheVal: 2.242 ± 0.023	0.392PheTrp: 0.392 ± 0.008	1.214PheTyr: 1.214 ± 0.015	0.001PheXaa: 0.001 ± 0.0
Gly	4.517GlyAla: 4.517 ± 0.06	1.075GlyCys: 1.075 ± 0.023	3.149GlyAsp: 3.149 ± 0.035	3.321GlyGlu: 3.321 ± 0.038	2.012GlyPhe: 2.012 ± 0.023	4.907GlyGly: 4.907 ± 0.091	1.4GlyHis: 1.4 ± 0.02	2.617GlyIle: 2.617 ± 0.027	3.078GlyLys: 3.078 ± 0.027	4.46GlyLeu: 4.46 ± 0.043	1.173GlyMet: 1.173 ± 0.017	2.373GlyAsn: 2.373 ± 0.03	2.763GlyPro: 2.763 ± 0.052	2.165GlyGln: 2.165 ± 0.043	3.334GlyArg: 3.334 ± 0.041	4.641GlySer: 4.641 ± 0.061	3.18GlyThr: 3.18 ± 0.032	3.776GlyVal: 3.776 ± 0.036	0.712GlyTrp: 0.712 ± 0.015	2.022GlyTyr: 2.022 ± 0.031	0.004GlyXaa: 0.004 ± 0.001
His	1.958HisAla: 1.958 ± 0.04	0.655HisCys: 0.655 ± 0.021	1.285HisAsp: 1.285 ± 0.023	1.409HisGlu: 1.409 ± 0.02	0.876HisPhe: 0.876 ± 0.012	1.374HisGly: 1.374 ± 0.022	1.148HisHis: 1.148 ± 0.06	1.304HisIle: 1.304 ± 0.018	1.396HisLys: 1.396 ± 0.018	2.242HisLeu: 2.242 ± 0.027	0.613HisMet: 0.613 ± 0.013	1.104HisAsn: 1.104 ± 0.015	1.356HisPro: 1.356 ± 0.022	1.015HisGln: 1.015 ± 0.025	1.471HisArg: 1.471 ± 0.018	1.928HisSer: 1.928 ± 0.021	1.551HisThr: 1.551 ± 0.03	1.704HisVal: 1.704 ± 0.032	0.289HisTrp: 0.289 ± 0.007	0.923HisTyr: 0.923 ± 0.016	0.001HisXaa: 0.001 ± 0.0
Ile	3.373IleAla: 3.373 ± 0.027	1.129IleCys: 1.129 ± 0.026	2.949IleAsp: 2.949 ± 0.026	3.359IleGlu: 3.359 ± 0.031	1.842IlePhe: 1.842 ± 0.023	2.515IleGly: 2.515 ± 0.029	1.199IleHis: 1.199 ± 0.019	2.822IleIle: 2.822 ± 0.027	3.44IleLys: 3.44 ± 0.044	4.335IleLeu: 4.335 ± 0.036	1.093IleMet: 1.093 ± 0.013	2.494IleAsn: 2.494 ± 0.026	2.697IlePro: 2.697 ± 0.028	1.99IleGln: 1.99 ± 0.024	2.448IleArg: 2.448 ± 0.024	3.786IleSer: 3.786 ± 0.029	3.145IleThr: 3.145 ± 0.032	3.307IleVal: 3.307 ± 0.029	0.473IleTrp: 0.473 ± 0.01	1.555IleTyr: 1.555 ± 0.02	0.002IleXaa: 0.002 ± 0.0
Lys	3.694LysAla: 3.694 ± 0.051	1.234LysCys: 1.234 ± 0.029	3.487LysAsp: 3.487 ± 0.039	4.765LysGlu: 4.765 ± 0.062	1.912LysPhe: 1.912 ± 0.02	2.633LysGly: 2.633 ± 0.03	1.527LysHis: 1.527 ± 0.02	3.363LysIle: 3.363 ± 0.031	5.219LysLys: 5.219 ± 0.059	5.406LysLeu: 5.406 ± 0.047	1.446LysMet: 1.446 ± 0.018	2.926LysAsn: 2.926 ± 0.032	3.673LysPro: 3.673 ± 0.073	2.505LysGln: 2.505 ± 0.029	3.556LysArg: 3.556 ± 0.033	4.378LysSer: 4.378 ± 0.043	3.616LysThr: 3.616 ± 0.047	3.691LysVal: 3.691 ± 0.037	0.594LysTrp: 0.594 ± 0.013	2.051LysTyr: 2.051 ± 0.02	0.003LysXaa: 0.003 ± 0.001
Leu	6.789LeuAla: 6.789 ± 0.058	1.841LeuCys: 1.841 ± 0.03	4.796LeuAsp: 4.796 ± 0.044	5.71LeuGlu: 5.71 ± 0.049	2.908LeuPhe: 2.908 ± 0.031	4.518LeuGly: 4.518 ± 0.044	2.38LeuHis: 2.38 ± 0.026	3.929LeuIle: 3.929 ± 0.031	5.556LeuLys: 5.556 ± 0.047	8.418LeuLeu: 8.418 ± 0.061	1.942LeuMet: 1.942 ± 0.024	3.863LeuAsn: 3.863 ± 0.033	4.998LeuPro: 4.998 ± 0.049	4.114LeuGln: 4.114 ± 0.036	5.374LeuArg: 5.374 ± 0.041	6.566LeuSer: 6.566 ± 0.046	4.889LeuThr: 4.889 ± 0.043	5.413LeuVal: 5.413 ± 0.043	0.876LeuTrp: 0.876 ± 0.014	2.562LeuTyr: 2.562 ± 0.026	0.003LeuXaa: 0.003 ± 0.001
Met	1.651MetAla: 1.651 ± 0.018	0.454MetCys: 0.454 ± 0.012	1.239MetAsp: 1.239 ± 0.015	1.52MetGlu: 1.52 ± 0.017	0.884MetPhe: 0.884 ± 0.012	1.127MetGly: 1.127 ± 0.022	0.528MetHis: 0.528 ± 0.011	0.976MetIle: 0.976 ± 0.013	1.446MetLys: 1.446 ± 0.016	1.953MetLeu: 1.953 ± 0.022	0.63MetMet: 0.63 ± 0.011	0.996MetAsn: 0.996 ± 0.013	1.179MetPro: 1.179 ± 0.019	0.951MetGln: 0.951 ± 0.015	1.232MetArg: 1.232 ± 0.016	1.771MetSer: 1.771 ± 0.018	1.214MetThr: 1.214 ± 0.014	1.274MetVal: 1.274 ± 0.017	0.251MetTrp: 0.251 ± 0.005	0.737MetTyr: 0.737 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.804AsnAla: 2.804 ± 0.032	0.881AsnCys: 0.881 ± 0.02	2.478AsnAsp: 2.478 ± 0.028	2.907AsnGlu: 2.907 ± 0.025	1.635AsnPhe: 1.635 ± 0.02	2.63AsnGly: 2.63 ± 0.027	0.972AsnHis: 0.972 ± 0.017	2.914AsnIle: 2.914 ± 0.028	3.053AsnLys: 3.053 ± 0.032	3.881AsnLeu: 3.881 ± 0.04	1.163AsnMet: 1.163 ± 0.018	2.711AsnAsn: 2.711 ± 0.03	2.216AsnPro: 2.216 ± 0.036	1.696AsnGln: 1.696 ± 0.023	2.083AsnArg: 2.083 ± 0.026	3.407AsnSer: 3.407 ± 0.034	2.761AsnThr: 2.761 ± 0.033	3.122AsnVal: 3.122 ± 0.029	0.454AsnTrp: 0.454 ± 0.011	1.565AsnTyr: 1.565 ± 0.019	0.001AsnXaa: 0.001 ± 0.0
Pro	4.808ProAla: 4.808 ± 0.059	0.982ProCys: 0.982 ± 0.052	3.218ProAsp: 3.218 ± 0.06	3.982ProGlu: 3.982 ± 0.041	1.57ProPhe: 1.57 ± 0.018	3.371ProGly: 3.371 ± 0.07	1.6ProHis: 1.6 ± 0.031	2.548ProIle: 2.548 ± 0.045	3.215ProLys: 3.215 ± 0.055	4.374ProLeu: 4.374 ± 0.038	1.033ProMet: 1.033 ± 0.016	2.306ProAsn: 2.306 ± 0.03	5.657ProPro: 5.657 ± 0.093	2.36ProGln: 2.36 ± 0.027	3.509ProArg: 3.509 ± 0.095	4.695ProSer: 4.695 ± 0.114	3.782ProThr: 3.782 ± 0.097	3.88ProVal: 3.88 ± 0.051	0.504ProTrp: 0.504 ± 0.009	1.623ProTyr: 1.623 ± 0.02	0.003ProXaa: 0.003 ± 0.001
Gln	2.811GlnAla: 2.811 ± 0.029	0.856GlnCys: 0.856 ± 0.023	1.837GlnAsp: 1.837 ± 0.021	2.533GlnGlu: 2.533 ± 0.025	1.264GlnPhe: 1.264 ± 0.017	1.925GlnGly: 1.925 ± 0.031	1.202GlnHis: 1.202 ± 0.023	1.966GlnIle: 1.966 ± 0.018	2.343GlnLys: 2.343 ± 0.024	3.77GlnLeu: 3.77 ± 0.035	1.01GlnMet: 1.01 ± 0.018	1.961GlnAsn: 1.961 ± 0.023	2.46GlnPro: 2.46 ± 0.036	2.558GlnGln: 2.558 ± 0.056	2.452GlnArg: 2.452 ± 0.033	2.728GlnSer: 2.728 ± 0.03	2.119GlnThr: 2.119 ± 0.025	2.342GlnVal: 2.342 ± 0.025	0.455GlnTrp: 0.455 ± 0.013	1.429GlnTyr: 1.429 ± 0.03	0.002GlnXaa: 0.002 ± 0.0
Arg	4.847ArgAla: 4.847 ± 0.075	1.448ArgCys: 1.448 ± 0.077	3.147ArgAsp: 3.147 ± 0.035	3.537ArgGlu: 3.537 ± 0.046	1.726ArgPhe: 1.726 ± 0.019	3.28ArgGly: 3.28 ± 0.038	1.709ArgHis: 1.709 ± 0.029	2.532ArgIle: 2.532 ± 0.027	3.525ArgLys: 3.525 ± 0.036	5.196ArgLeu: 5.196 ± 0.05	1.148ArgMet: 1.148 ± 0.014	2.423ArgAsn: 2.423 ± 0.027	3.274ArgPro: 3.274 ± 0.043	2.307ArgGln: 2.307 ± 0.025	5.082ArgArg: 5.082 ± 0.071	4.223ArgSer: 4.223 ± 0.045	3.057ArgThr: 3.057 ± 0.036	3.493ArgVal: 3.493 ± 0.039	0.661ArgTrp: 0.661 ± 0.01	1.784ArgTyr: 1.784 ± 0.027	0.003ArgXaa: 0.003 ± 0.001
Ser	5.319SerAla: 5.319 ± 0.045	1.519SerCys: 1.519 ± 0.044	4.506SerAsp: 4.506 ± 0.037	4.724SerGlu: 4.724 ± 0.035	2.4SerPhe: 2.4 ± 0.025	4.673SerGly: 4.673 ± 0.042	1.735SerHis: 1.735 ± 0.023	3.545SerIle: 3.545 ± 0.033	4.29SerLys: 4.29 ± 0.038	6.381SerLeu: 6.381 ± 0.045	1.565SerMet: 1.565 ± 0.017	3.482SerAsn: 3.482 ± 0.033	5.064SerPro: 5.064 ± 0.119	2.874SerGln: 2.874 ± 0.035	4.156SerArg: 4.156 ± 0.04	7.456SerSer: 7.456 ± 0.069	4.893SerThr: 4.893 ± 0.059	4.775SerVal: 4.775 ± 0.038	0.777SerTrp: 0.777 ± 0.011	2.157SerTyr: 2.157 ± 0.027	0.003SerXaa: 0.003 ± 0.001
Thr	4.282ThrAla: 4.282 ± 0.045	1.28ThrCys: 1.28 ± 0.029	3.326ThrAsp: 3.326 ± 0.037	3.827ThrGlu: 3.827 ± 0.053	2.019ThrPhe: 2.019 ± 0.022	3.229ThrGly: 3.229 ± 0.034	1.446ThrHis: 1.446 ± 0.025	3.021ThrIle: 3.021 ± 0.029	3.31ThrLys: 3.31 ± 0.035	5.134ThrLeu: 5.134 ± 0.035	1.2ThrMet: 1.2 ± 0.014	2.549ThrAsn: 2.549 ± 0.026	4.105ThrPro: 4.105 ± 0.057	2.249ThrGln: 2.249 ± 0.04	2.94ThrArg: 2.94 ± 0.037	4.801ThrSer: 4.801 ± 0.047	4.792ThrThr: 4.792 ± 0.213	4.09ThrVal: 4.09 ± 0.038	0.621ThrTrp: 0.621 ± 0.013	1.728ThrTyr: 1.728 ± 0.024	0.001ThrXaa: 0.001 ± 0.0
Val	4.826ValAla: 4.826 ± 0.044	1.543ValCys: 1.543 ± 0.031	3.498ValAsp: 3.498 ± 0.032	4.109ValGlu: 4.109 ± 0.034	2.225ValPhe: 2.225 ± 0.025	3.294ValGly: 3.294 ± 0.036	1.616ValHis: 1.616 ± 0.026	3.278ValIle: 3.278 ± 0.033	3.885ValLys: 3.885 ± 0.064	5.887ValLeu: 5.887 ± 0.049	1.384ValMet: 1.384 ± 0.018	2.873ValAsn: 2.873 ± 0.028	3.734ValPro: 3.734 ± 0.048	2.521ValGln: 2.521 ± 0.026	3.538ValArg: 3.538 ± 0.037	4.786ValSer: 4.786 ± 0.034	4.214ValThr: 4.214 ± 0.039	4.475ValVal: 4.475 ± 0.039	0.731ValTrp: 0.731 ± 0.014	1.933ValTyr: 1.933 ± 0.02	0.001ValXaa: 0.001 ± 0.0
Trp	0.687TrpAla: 0.687 ± 0.012	0.252TrpCys: 0.252 ± 0.008	0.57TrpAsp: 0.57 ± 0.01	0.624TrpGlu: 0.624 ± 0.012	0.401TrpPhe: 0.401 ± 0.008	0.6TrpGly: 0.6 ± 0.014	0.259TrpHis: 0.259 ± 0.007	0.532TrpIle: 0.532 ± 0.012	0.621TrpLys: 0.621 ± 0.012	1.077TrpLeu: 1.077 ± 0.018	0.263TrpMet: 0.263 ± 0.005	0.475TrpAsn: 0.475 ± 0.008	0.476TrpPro: 0.476 ± 0.011	0.428TrpGln: 0.428 ± 0.007	0.854TrpArg: 0.854 ± 0.022	0.803TrpSer: 0.803 ± 0.012	0.599TrpThr: 0.599 ± 0.012	0.62TrpVal: 0.62 ± 0.01	0.204TrpTrp: 0.204 ± 0.006	0.359TrpTyr: 0.359 ± 0.008	0.001TrpXaa: 0.001 ± 0.0
Tyr	1.895TyrAla: 1.895 ± 0.026	0.743TyrCys: 0.743 ± 0.012	1.816TyrAsp: 1.816 ± 0.021	1.927TyrGlu: 1.927 ± 0.022	1.238TyrPhe: 1.238 ± 0.017	1.91TyrGly: 1.91 ± 0.027	0.852TyrHis: 0.852 ± 0.017	1.686TyrIle: 1.686 ± 0.025	1.819TyrLys: 1.819 ± 0.019	2.725TyrLeu: 2.725 ± 0.026	0.745TyrMet: 0.745 ± 0.012	1.573TyrAsn: 1.573 ± 0.018	1.579TyrPro: 1.579 ± 0.035	1.222TyrGln: 1.222 ± 0.017	1.706TyrArg: 1.706 ± 0.017	2.303TyrSer: 2.303 ± 0.026	1.91TyrThr: 1.91 ± 0.026	1.977TyrVal: 1.977 ± 0.021	0.372TyrTrp: 0.372 ± 0.008	1.219TyrTyr: 1.219 ± 0.017	0.001TyrXaa: 0.001 ± 0.0
Xaa	0.003XaaAla: 0.003 ± 0.001	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.002XaaPhe: 0.002 ± 0.0	0.002XaaGly: 0.002 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.002XaaIle: 0.002 ± 0.001	0.003XaaLys: 0.003 ± 0.001	0.004XaaLeu: 0.004 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.003XaaPro: 0.003 ± 0.001	0.001XaaGln: 0.001 ± 0.0	0.003XaaArg: 0.003 ± 0.0	0.003XaaSer: 0.003 ± 0.001	0.002XaaThr: 0.002 ± 0.0	0.002XaaVal: 0.002 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.085XaaXaa: 0.085 ± 0.018

Statistics based on 17055 proteins (9040933 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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