Flavobacterium phage VK20

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ficleduovirus; unclassified Ficleduovirus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218M7Z8|A0A218M7Z8_9CAUD Uncharacterized protein OS=Flavobacterium phage VK20 OX=1984753 PE=4 SV=1
MM1 pKa = 8.28LEE3 pKa = 3.85IFFTINGIDD12 pKa = 3.38QKK14 pKa = 10.67EE15 pKa = 3.91IAVFYY20 pKa = 10.96DD21 pKa = 3.81NEE23 pKa = 4.04DD24 pKa = 2.72LKK26 pKa = 11.6YY27 pKa = 10.41FVEE30 pKa = 4.98TYY32 pKa = 10.32KK33 pKa = 10.93EE34 pKa = 4.05QVLEE38 pKa = 4.09QYY40 pKa = 7.58PTATNIRR47 pKa = 11.84LGDD50 pKa = 3.94LSTSLNNYY58 pKa = 7.79NAA60 pKa = 4.02

Molecular weight: 7.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A218M7X3|A0A218M7X3_9CAUD Uncharacterized protein OS=Flavobacterium phage VK20 OX=1984753 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84AWNANLRR9 pKa = 11.84LKK11 pKa = 10.72ANRR14 pKa = 11.84VVYY17 pKa = 10.17RR18 pKa = 11.84AYY20 pKa = 8.46THH22 pKa = 6.43NMVKK26 pKa = 8.85PTGTTDD32 pKa = 3.4YY33 pKa = 10.89VSVSFVLPVKK43 pKa = 10.66SDD45 pKa = 3.41FLANGGLLYY54 pKa = 10.87VNPNTGITITNRR66 pKa = 11.84YY67 pKa = 7.56IAAGLLHH74 pKa = 6.79KK75 pKa = 10.68GVV77 pKa = 3.71

Molecular weight: 8.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

14557

34

729

196.7

22.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.132 ± 0.425

0.934 ± 0.114

5.75 ± 0.214

7.055 ± 0.426

4.788 ± 0.203

5.489 ± 0.327

1.285 ± 0.116

7.866 ± 0.219

9.48 ± 0.451

9.006 ± 0.244

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.164 ± 0.162

7.309 ± 0.287

2.885 ± 0.176

3.661 ± 0.181

3.483 ± 0.212

6.595 ± 0.246

5.873 ± 0.264

5.832 ± 0.196

1.085 ± 0.103

4.328 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski