Amino acid dipepetide frequency for Lysobacter silvestris

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.795AlaAla: 15.795 ± 0.216
1.237AlaCys: 1.237 ± 0.034
6.779AlaAsp: 6.779 ± 0.101
5.952AlaGlu: 5.952 ± 0.108
3.899AlaPhe: 3.899 ± 0.068
10.045AlaGly: 10.045 ± 0.159
2.728AlaHis: 2.728 ± 0.067
6.329AlaIle: 6.329 ± 0.093
4.176AlaLys: 4.176 ± 0.103
14.302AlaLeu: 14.302 ± 0.175
3.553AlaMet: 3.553 ± 0.066
3.584AlaAsn: 3.584 ± 0.092
5.599AlaPro: 5.599 ± 0.092
5.104AlaGln: 5.104 ± 0.088
8.782AlaArg: 8.782 ± 0.133
6.664AlaSer: 6.664 ± 0.097
6.599AlaThr: 6.599 ± 0.125
8.087AlaVal: 8.087 ± 0.118
2.008AlaTrp: 2.008 ± 0.05
2.463AlaTyr: 2.463 ± 0.049
0.0AlaXaa: 0.0 ± 0.0
Cys
1.051CysAla: 1.051 ± 0.037
0.104CysCys: 0.104 ± 0.011
0.486CysAsp: 0.486 ± 0.023
0.437CysGlu: 0.437 ± 0.02
0.265CysPhe: 0.265 ± 0.016
0.941CysGly: 0.941 ± 0.033
0.191CysHis: 0.191 ± 0.013
0.446CysIle: 0.446 ± 0.019
0.245CysLys: 0.245 ± 0.017
0.746CysLeu: 0.746 ± 0.03
0.19CysMet: 0.19 ± 0.016
0.245CysAsn: 0.245 ± 0.018
0.452CysPro: 0.452 ± 0.025
0.222CysGln: 0.222 ± 0.015
0.513CysArg: 0.513 ± 0.026
0.499CysSer: 0.499 ± 0.024
0.469CysThr: 0.469 ± 0.023
0.674CysVal: 0.674 ± 0.03
0.096CysTrp: 0.096 ± 0.01
0.201CysTyr: 0.201 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
9.024AspAla: 9.024 ± 0.138
0.433AspCys: 0.433 ± 0.022
3.799AspAsp: 3.799 ± 0.072
3.204AspGlu: 3.204 ± 0.068
2.143AspPhe: 2.143 ± 0.052
6.093AspGly: 6.093 ± 0.101
1.445AspHis: 1.445 ± 0.041
2.867AspIle: 2.867 ± 0.053
1.736AspLys: 1.736 ± 0.055
5.563AspLeu: 5.563 ± 0.089
1.252AspMet: 1.252 ± 0.039
1.48AspAsn: 1.48 ± 0.046
3.507AspPro: 3.507 ± 0.078
1.676AspGln: 1.676 ± 0.043
4.016AspArg: 4.016 ± 0.063
2.682AspSer: 2.682 ± 0.054
2.798AspThr: 2.798 ± 0.058
4.732AspVal: 4.732 ± 0.08
1.114AspTrp: 1.114 ± 0.037
1.695AspTyr: 1.695 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
5.954GluAla: 5.954 ± 0.113
0.36GluCys: 0.36 ± 0.023
2.4GluAsp: 2.4 ± 0.064
2.234GluGlu: 2.234 ± 0.07
1.802GluPhe: 1.802 ± 0.048
3.444GluGly: 3.444 ± 0.074
1.489GluHis: 1.489 ± 0.044
2.504GluIle: 2.504 ± 0.06
1.529GluLys: 1.529 ± 0.05
5.81GluLeu: 5.81 ± 0.094
1.166GluMet: 1.166 ± 0.042
1.148GluAsn: 1.148 ± 0.039
2.142GluPro: 2.142 ± 0.05
2.308GluGln: 2.308 ± 0.061
4.682GluArg: 4.682 ± 0.096
2.582GluSer: 2.582 ± 0.057
2.659GluThr: 2.659 ± 0.065
3.533GluVal: 3.533 ± 0.065
0.74GluTrp: 0.74 ± 0.029
1.152GluTyr: 1.152 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
4.344PheAla: 4.344 ± 0.07
0.329PheCys: 0.329 ± 0.019
2.835PheAsp: 2.835 ± 0.054
1.758PheGlu: 1.758 ± 0.049
1.165PhePhe: 1.165 ± 0.046
3.252PheGly: 3.252 ± 0.069
0.867PheHis: 0.867 ± 0.032
1.471PheIle: 1.471 ± 0.041
1.033PheLys: 1.033 ± 0.04
2.909PheLeu: 2.909 ± 0.065
0.672PheMet: 0.672 ± 0.025
1.168PheAsn: 1.168 ± 0.036
1.48PhePro: 1.48 ± 0.041
0.909PheGln: 0.909 ± 0.035
2.13PheArg: 2.13 ± 0.051
1.981PheSer: 1.981 ± 0.056
1.87PheThr: 1.87 ± 0.055
2.526PheVal: 2.526 ± 0.062
0.487PheTrp: 0.487 ± 0.025
0.827PheTyr: 0.827 ± 0.033
0.0PheXaa: 0.0 ± 0.0
Gly
8.693GlyAla: 8.693 ± 0.214
0.816GlyCys: 0.816 ± 0.03
5.242GlyAsp: 5.242 ± 0.075
4.425GlyGlu: 4.425 ± 0.08
3.32GlyPhe: 3.32 ± 0.068
7.255GlyGly: 7.255 ± 0.242
2.175GlyHis: 2.175 ± 0.056
4.502GlyIle: 4.502 ± 0.079
3.739GlyLys: 3.739 ± 0.064
8.079GlyLeu: 8.079 ± 0.104
2.401GlyMet: 2.401 ± 0.054
2.798GlyAsn: 2.798 ± 0.105
2.815GlyPro: 2.815 ± 0.052
3.061GlyGln: 3.061 ± 0.061
5.59GlyArg: 5.59 ± 0.095
4.901GlySer: 4.901 ± 0.127
4.623GlyThr: 4.623 ± 0.162
6.249GlyVal: 6.249 ± 0.096
1.599GlyTrp: 1.599 ± 0.043
2.422GlyTyr: 2.422 ± 0.064
0.0GlyXaa: 0.0 ± 0.0
His
3.265HisAla: 3.265 ± 0.075
0.278HisCys: 0.278 ± 0.018
1.609HisAsp: 1.609 ± 0.047
1.129HisGlu: 1.129 ± 0.035
0.836HisPhe: 0.836 ± 0.033
2.542HisGly: 2.542 ± 0.061
0.72HisHis: 0.72 ± 0.032
0.959HisIle: 0.959 ± 0.032
0.55HisLys: 0.55 ± 0.025
2.288HisLeu: 2.288 ± 0.058
0.527HisMet: 0.527 ± 0.026
0.542HisAsn: 0.542 ± 0.025
1.587HisPro: 1.587 ± 0.045
0.685HisGln: 0.685 ± 0.029
1.752HisArg: 1.752 ± 0.055
1.114HisSer: 1.114 ± 0.037
0.98HisThr: 0.98 ± 0.037
1.822HisVal: 1.822 ± 0.049
0.471HisTrp: 0.471 ± 0.022
0.706HisTyr: 0.706 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
7.335IleAla: 7.335 ± 0.1
0.382IleCys: 0.382 ± 0.021
3.936IleAsp: 3.936 ± 0.068
3.136IleGlu: 3.136 ± 0.065
1.248IlePhe: 1.248 ± 0.045
4.758IleGly: 4.758 ± 0.082
1.075IleHis: 1.075 ± 0.035
1.7IleIle: 1.7 ± 0.06
1.274IleLys: 1.274 ± 0.048
3.516IleLeu: 3.516 ± 0.072
0.6IleMet: 0.6 ± 0.03
1.459IleAsn: 1.459 ± 0.052
2.492IlePro: 2.492 ± 0.057
1.205IleGln: 1.205 ± 0.039
3.078IleArg: 3.078 ± 0.064
2.353IleSer: 2.353 ± 0.055
2.618IleThr: 2.618 ± 0.063
3.582IleVal: 3.582 ± 0.07
0.452IleTrp: 0.452 ± 0.026
0.973IleTyr: 0.973 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.651LysAla: 3.651 ± 0.098
0.154LysCys: 0.154 ± 0.015
1.82LysAsp: 1.82 ± 0.054
1.329LysGlu: 1.329 ± 0.054
0.943LysPhe: 0.943 ± 0.041
2.288LysGly: 2.288 ± 0.054
0.792LysHis: 0.792 ± 0.03
1.499LysIle: 1.499 ± 0.05
1.265LysLys: 1.265 ± 0.065
3.493LysLeu: 3.493 ± 0.062
0.788LysMet: 0.788 ± 0.029
0.917LysAsn: 0.917 ± 0.042
2.184LysPro: 2.184 ± 0.063
1.397LysGln: 1.397 ± 0.04
2.44LysArg: 2.44 ± 0.053
1.854LysSer: 1.854 ± 0.042
1.919LysThr: 1.919 ± 0.052
2.389LysVal: 2.389 ± 0.056
0.384LysTrp: 0.384 ± 0.023
0.774LysTyr: 0.774 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
13.658LeuAla: 13.658 ± 0.161
0.947LeuCys: 0.947 ± 0.035
6.782LeuAsp: 6.782 ± 0.087
4.901LeuGlu: 4.901 ± 0.092
3.343LeuPhe: 3.343 ± 0.064
8.26LeuGly: 8.26 ± 0.15
2.621LeuHis: 2.621 ± 0.06
4.133LeuIle: 4.133 ± 0.073
3.339LeuLys: 3.339 ± 0.078
10.716LeuLeu: 10.716 ± 0.171
2.22LeuMet: 2.22 ± 0.054
2.43LeuAsn: 2.43 ± 0.093
6.086LeuPro: 6.086 ± 0.091
3.995LeuGln: 3.995 ± 0.073
8.216LeuArg: 8.216 ± 0.119
5.762LeuSer: 5.762 ± 0.094
4.638LeuThr: 4.638 ± 0.104
7.267LeuVal: 7.267 ± 0.111
1.293LeuTrp: 1.293 ± 0.037
2.064LeuTyr: 2.064 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
2.816MetAla: 2.816 ± 0.059
0.173MetCys: 0.173 ± 0.014
1.214MetAsp: 1.214 ± 0.037
1.029MetGlu: 1.029 ± 0.037
0.712MetPhe: 0.712 ± 0.033
1.651MetGly: 1.651 ± 0.047
0.579MetHis: 0.579 ± 0.026
1.034MetIle: 1.034 ± 0.033
0.961MetLys: 0.961 ± 0.04
2.59MetLeu: 2.59 ± 0.065
0.613MetMet: 0.613 ± 0.027
0.827MetAsn: 0.827 ± 0.029
1.533MetPro: 1.533 ± 0.047
1.053MetGln: 1.053 ± 0.038
1.831MetArg: 1.831 ± 0.043
1.595MetSer: 1.595 ± 0.038
1.548MetThr: 1.548 ± 0.04
1.594MetVal: 1.594 ± 0.05
0.231MetTrp: 0.231 ± 0.018
0.408MetTyr: 0.408 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.723AsnAla: 3.723 ± 0.098
0.238AsnCys: 0.238 ± 0.017
1.761AsnAsp: 1.761 ± 0.045
1.251AsnGlu: 1.251 ± 0.041
0.97AsnPhe: 0.97 ± 0.035
2.934AsnGly: 2.934 ± 0.127
0.595AsnHis: 0.595 ± 0.028
1.334AsnIle: 1.334 ± 0.04
0.824AsnLys: 0.824 ± 0.035
2.503AsnLeu: 2.503 ± 0.075
0.51AsnMet: 0.51 ± 0.022
0.901AsnAsn: 0.901 ± 0.048
1.947AsnPro: 1.947 ± 0.049
0.831AsnGln: 0.831 ± 0.034
1.785AsnArg: 1.785 ± 0.045
1.397AsnSer: 1.397 ± 0.051
1.639AsnThr: 1.639 ± 0.069
2.111AsnVal: 2.111 ± 0.07
0.43AsnTrp: 0.43 ± 0.022
0.795AsnTyr: 0.795 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
6.643ProAla: 6.643 ± 0.108
0.322ProCys: 0.322 ± 0.016
3.437ProAsp: 3.437 ± 0.06
3.068ProGlu: 3.068 ± 0.067
1.632ProPhe: 1.632 ± 0.043
4.493ProGly: 4.493 ± 0.078
1.128ProHis: 1.128 ± 0.04
2.199ProIle: 2.199 ± 0.049
1.692ProLys: 1.692 ± 0.047
5.045ProLeu: 5.045 ± 0.083
1.375ProMet: 1.375 ± 0.042
1.415ProAsn: 1.415 ± 0.042
2.481ProPro: 2.481 ± 0.076
2.229ProGln: 2.229 ± 0.053
3.22ProArg: 3.22 ± 0.071
2.65ProSer: 2.65 ± 0.055
2.654ProThr: 2.654 ± 0.074
4.008ProVal: 4.008 ± 0.076
0.845ProTrp: 0.845 ± 0.035
1.233ProTyr: 1.233 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
4.719GlnAla: 4.719 ± 0.084
0.27GlnCys: 0.27 ± 0.018
1.61GlnAsp: 1.61 ± 0.051
1.455GlnGlu: 1.455 ± 0.045
1.264GlnPhe: 1.264 ± 0.039
2.679GlnGly: 2.679 ± 0.06
0.955GlnHis: 0.955 ± 0.036
1.617GlnIle: 1.617 ± 0.048
1.079GlnLys: 1.079 ± 0.044
4.071GlnLeu: 4.071 ± 0.073
0.869GlnMet: 0.869 ± 0.028
0.854GlnAsn: 0.854 ± 0.039
2.208GlnPro: 2.208 ± 0.06
1.934GlnGln: 1.934 ± 0.063
3.615GlnArg: 3.615 ± 0.07
2.003GlnSer: 2.003 ± 0.053
1.738GlnThr: 1.738 ± 0.041
2.818GlnVal: 2.818 ± 0.059
0.619GlnTrp: 0.619 ± 0.032
0.827GlnTyr: 0.827 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
7.895ArgAla: 7.895 ± 0.122
0.5ArgCys: 0.5 ± 0.022
4.971ArgAsp: 4.971 ± 0.082
4.334ArgGlu: 4.334 ± 0.08
2.781ArgPhe: 2.781 ± 0.054
5.341ArgGly: 5.341 ± 0.09
1.91ArgHis: 1.91 ± 0.053
4.244ArgIle: 4.244 ± 0.066
2.436ArgLys: 2.436 ± 0.064
7.277ArgLeu: 7.277 ± 0.128
2.088ArgMet: 2.088 ± 0.049
2.161ArgAsn: 2.161 ± 0.044
3.069ArgPro: 3.069 ± 0.07
2.567ArgGln: 2.567 ± 0.06
5.208ArgArg: 5.208 ± 0.09
3.622ArgSer: 3.622 ± 0.063
3.272ArgThr: 3.272 ± 0.06
5.099ArgVal: 5.099 ± 0.088
1.3ArgTrp: 1.3 ± 0.038
2.151ArgTyr: 2.151 ± 0.059
0.0ArgXaa: 0.0 ± 0.0
Ser
6.265SerAla: 6.265 ± 0.095
0.421SerCys: 0.421 ± 0.021
3.206SerAsp: 3.206 ± 0.061
2.488SerGlu: 2.488 ± 0.06
1.907SerPhe: 1.907 ± 0.054
5.679SerGly: 5.679 ± 0.149
1.278SerHis: 1.278 ± 0.042
2.448SerIle: 2.448 ± 0.052
1.66SerLys: 1.66 ± 0.044
5.292SerLeu: 5.292 ± 0.101
1.253SerMet: 1.253 ± 0.045
1.716SerAsn: 1.716 ± 0.057
2.953SerPro: 2.953 ± 0.061
1.875SerGln: 1.875 ± 0.043
3.641SerArg: 3.641 ± 0.067
2.983SerSer: 2.983 ± 0.078
2.993SerThr: 2.993 ± 0.086
3.656SerVal: 3.656 ± 0.065
0.797SerTrp: 0.797 ± 0.034
1.347SerTyr: 1.347 ± 0.05
0.0SerXaa: 0.0 ± 0.0
Thr
5.767ThrAla: 5.767 ± 0.121
0.47ThrCys: 0.47 ± 0.029
2.486ThrAsp: 2.486 ± 0.06
1.958ThrGlu: 1.958 ± 0.051
1.743ThrPhe: 1.743 ± 0.055
4.626ThrGly: 4.626 ± 0.131
1.272ThrHis: 1.272 ± 0.044
2.604ThrIle: 2.604 ± 0.072
1.369ThrLys: 1.369 ± 0.054
6.299ThrLeu: 6.299 ± 0.164
1.198ThrMet: 1.198 ± 0.043
1.371ThrAsn: 1.371 ± 0.051
3.616ThrPro: 3.616 ± 0.065
1.898ThrGln: 1.898 ± 0.061
3.447ThrArg: 3.447 ± 0.068
2.866ThrSer: 2.866 ± 0.088
3.222ThrThr: 3.222 ± 0.096
4.071ThrVal: 4.071 ± 0.109
0.748ThrTrp: 0.748 ± 0.031
1.32ThrTyr: 1.32 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
9.006ValAla: 9.006 ± 0.117
0.661ValCys: 0.661 ± 0.029
4.662ValAsp: 4.662 ± 0.079
3.974ValGlu: 3.974 ± 0.088
2.533ValPhe: 2.533 ± 0.056
5.288ValGly: 5.288 ± 0.096
1.64ValHis: 1.64 ± 0.044
3.658ValIle: 3.658 ± 0.071
2.15ValLys: 2.15 ± 0.055
7.619ValLeu: 7.619 ± 0.105
1.76ValMet: 1.76 ± 0.053
2.128ValAsn: 2.128 ± 0.063
3.696ValPro: 3.696 ± 0.069
2.598ValGln: 2.598 ± 0.055
4.969ValArg: 4.969 ± 0.096
4.026ValSer: 4.026 ± 0.075
4.009ValThr: 4.009 ± 0.101
6.058ValVal: 6.058 ± 0.088
0.866ValTrp: 0.866 ± 0.034
1.497ValTyr: 1.497 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
1.196TrpAla: 1.196 ± 0.039
0.159TrpCys: 0.159 ± 0.014
0.691TrpAsp: 0.691 ± 0.03
0.507TrpGlu: 0.507 ± 0.021
0.589TrpPhe: 0.589 ± 0.029
0.933TrpGly: 0.933 ± 0.034
0.384TrpHis: 0.384 ± 0.02
0.768TrpIle: 0.768 ± 0.03
0.583TrpLys: 0.583 ± 0.026
2.198TrpLeu: 2.198 ± 0.055
0.52TrpMet: 0.52 ± 0.026
0.579TrpAsn: 0.579 ± 0.024
0.76TrpPro: 0.76 ± 0.031
0.727TrpGln: 0.727 ± 0.029
1.312TrpArg: 1.312 ± 0.04
0.956TrpSer: 0.956 ± 0.039
0.791TrpThr: 0.791 ± 0.034
0.85TrpVal: 0.85 ± 0.029
0.332TrpTrp: 0.332 ± 0.022
0.352TrpTyr: 0.352 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.885TyrAla: 2.885 ± 0.058
0.216TyrCys: 0.216 ± 0.018
1.531TyrAsp: 1.531 ± 0.05
1.071TyrGlu: 1.071 ± 0.039
0.917TyrPhe: 0.917 ± 0.031
2.205TyrGly: 2.205 ± 0.057
0.518TyrHis: 0.518 ± 0.024
0.854TyrIle: 0.854 ± 0.036
0.644TyrLys: 0.644 ± 0.032
2.353TyrLeu: 2.353 ± 0.055
0.436TyrMet: 0.436 ± 0.021
0.758TyrAsn: 0.758 ± 0.039
1.161TyrPro: 1.161 ± 0.042
0.927TyrGln: 0.927 ± 0.041
1.999TyrArg: 1.999 ± 0.053
1.371TyrSer: 1.371 ± 0.048
1.309TyrThr: 1.309 ± 0.051
1.673TyrVal: 1.673 ± 0.042
0.376TyrTrp: 0.376 ± 0.023
0.668TyrTyr: 0.668 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2544 proteins (874491 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski