Solimonas fluminis
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4276 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S5TK49|A0A2S5TK49_9GAMM Urease subunit gamma OS=Solimonas fluminis OX=2086571 GN=ureA PE=3 SV=1
MM1 pKa = 7.12 NRR3 pKa = 11.84 VGKK6 pKa = 8.21 ATRR9 pKa = 11.84 WASALMAVGAAGAMPVAQADD29 pKa = 3.73 IEE31 pKa = 4.47 LGNGLSVTGFLDD43 pKa = 3.34 MSYY46 pKa = 11.26 SSVNPDD52 pKa = 2.98 SGSSTEE58 pKa = 4.6 SVGIDD63 pKa = 3.41 QFEE66 pKa = 4.24 MDD68 pKa = 4.69 FKK70 pKa = 10.74 YY71 pKa = 10.52 AGSGGVSAQVDD82 pKa = 3.42 IEE84 pKa = 4.22 YY85 pKa = 11.24 GEE87 pKa = 4.76 GFDD90 pKa = 5.35 GSDD93 pKa = 3.42 DD94 pKa = 3.68 EE95 pKa = 5.05 TFVEE99 pKa = 4.49 QAFITKK105 pKa = 10.32 AFTEE109 pKa = 4.26 KK110 pKa = 10.64 FSVKK114 pKa = 10.11 AGRR117 pKa = 11.84 FLSYY121 pKa = 10.62 SGWEE125 pKa = 4.17 TEE127 pKa = 4.12 EE128 pKa = 3.92 PTGLFQYY135 pKa = 10.72 SGTGYY140 pKa = 10.75 AKK142 pKa = 10.45 YY143 pKa = 10.07 FYY145 pKa = 10.46 GYY147 pKa = 8.58 YY148 pKa = 9.85 QNGVSAYY155 pKa = 9.1 YY156 pKa = 10.43 NGGKK160 pKa = 9.13 FAVMGSAVTSAFNPNDD176 pKa = 3.64 RR177 pKa = 11.84 NNVEE181 pKa = 4.24 GVDD184 pKa = 3.74 DD185 pKa = 4.08 KK186 pKa = 11.5 KK187 pKa = 11.32 GYY189 pKa = 10.25 EE190 pKa = 3.97 FGLAVMPIEE199 pKa = 4.54 GLTAKK204 pKa = 10.48 AFYY207 pKa = 10.36 IADD210 pKa = 3.91 NDD212 pKa = 3.77 TDD214 pKa = 3.67 TDD216 pKa = 4.21 IINVWVSYY224 pKa = 10.69 AVAGFTFAGEE234 pKa = 4.4 YY235 pKa = 8.67 NTVEE239 pKa = 4.33 YY240 pKa = 10.23 GDD242 pKa = 3.92 SSAVVDD248 pKa = 4.3 GDD250 pKa = 3.64 GDD252 pKa = 4.27 GYY254 pKa = 11.79 LLMANYY260 pKa = 10.73 AKK262 pKa = 10.43 GPYY265 pKa = 9.91 GITLRR270 pKa = 11.84 YY271 pKa = 9.32 HH272 pKa = 6.92 DD273 pKa = 6.08 FEE275 pKa = 5.36 IQDD278 pKa = 3.55 AGGATVDD285 pKa = 4.31 DD286 pKa = 5.07 GSAITLSPSYY296 pKa = 10.56 KK297 pKa = 10.35 VADD300 pKa = 3.62 NLLLVGEE307 pKa = 4.26 YY308 pKa = 10.76 RR309 pKa = 11.84 MDD311 pKa = 3.68 EE312 pKa = 4.12 SDD314 pKa = 3.65 TNGDD318 pKa = 3.38 SDD320 pKa = 4.01 SFAIEE325 pKa = 3.79 ALFTFF330 pKa = 5.15
Molecular weight: 35.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.872
IPC_protein 3.872
Toseland 3.656
ProMoST 4.024
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.694
Grimsley 3.567
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.202
Thurlkill 3.706
EMBOSS 3.795
Sillero 3.999
Patrickios 0.693
IPC_peptide 3.846
IPC2_peptide 3.973
IPC2.peptide.svr19 3.876
Protein with the highest isoelectric point:
>tr|A0A2S5TJZ1|A0A2S5TJZ1_9GAMM Enoyl-CoA hydratase OS=Solimonas fluminis OX=2086571 GN=C3942_06335 PE=3 SV=1
MM1 pKa = 6.59 ATKK4 pKa = 9.57 RR5 pKa = 11.84 TFQPKK10 pKa = 8.81 KK11 pKa = 8.99 LRR13 pKa = 11.84 RR14 pKa = 11.84 ARR16 pKa = 11.84 THH18 pKa = 5.92 GFRR21 pKa = 11.84 ARR23 pKa = 11.84 MATVGGRR30 pKa = 11.84 KK31 pKa = 9.31 VISRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 9.94 GRR41 pKa = 11.84 KK42 pKa = 8.89 RR43 pKa = 11.84 LIPP46 pKa = 4.02
Molecular weight: 5.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4276
0
4276
1399617
29
4449
327.3
35.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.38 ± 0.054
0.953 ± 0.013
5.316 ± 0.029
5.809 ± 0.036
3.444 ± 0.022
8.81 ± 0.047
2.092 ± 0.021
4.189 ± 0.028
3.128 ± 0.034
11.069 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.242 ± 0.021
2.531 ± 0.026
5.578 ± 0.036
3.926 ± 0.022
7.666 ± 0.038
5.267 ± 0.034
4.629 ± 0.039
7.006 ± 0.031
1.483 ± 0.017
2.481 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here