Streptococcus satellite phage Javan378
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZL97|A0A4D5ZL97_9VIRU HTH merR-type domain-containing protein OS=Streptococcus satellite phage Javan378 OX=2558668 GN=JavanS378_0006 PE=4 SV=1
MM1 pKa = 7.5 NKK3 pKa = 8.89 TEE5 pKa = 5.64 LINKK9 pKa = 8.59 IDD11 pKa = 3.49 KK12 pKa = 10.92 AMNRR16 pKa = 11.84 LDD18 pKa = 3.71 FLAEE22 pKa = 3.59 IMEE25 pKa = 4.43 GEE27 pKa = 4.26 KK28 pKa = 10.05 TLTAGLNNISDD39 pKa = 3.7 ILFEE43 pKa = 4.17 IRR45 pKa = 11.84 GEE47 pKa = 3.8 ISLNGIKK54 pKa = 10.41 GDD56 pKa = 3.89 EE57 pKa = 4.19 NVV59 pKa = 3.36
Molecular weight: 6.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.834
IPC2_protein 4.698
IPC_protein 4.482
Toseland 4.342
ProMoST 4.584
Dawson 4.418
Bjellqvist 4.571
Wikipedia 4.291
Rodwell 4.329
Grimsley 4.253
Solomon 4.418
Lehninger 4.368
Nozaki 4.533
DTASelect 4.647
Thurlkill 4.342
EMBOSS 4.304
Sillero 4.584
Patrickios 4.495
IPC_peptide 4.418
IPC2_peptide 4.584
IPC2.peptide.svr19 4.525
Protein with the highest isoelectric point:
>tr|A0A4D5ZM52|A0A4D5ZM52_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan378 OX=2558668 GN=JavanS378_0008 PE=4 SV=1
MM1 pKa = 7.79 NITEE5 pKa = 4.26 YY6 pKa = 10.61 KK7 pKa = 10.28 KK8 pKa = 10.39 KK9 pKa = 10.61 DD10 pKa = 3.14 GTIVYY15 pKa = 7.65 RR16 pKa = 11.84 TSLYY20 pKa = 10.84 LGIDD24 pKa = 3.71 SITGKK29 pKa = 10.31 KK30 pKa = 9.62 IKK32 pKa = 8.03 TTISARR38 pKa = 11.84 TKK40 pKa = 10.83 KK41 pKa = 10.02 EE42 pKa = 3.75 IKK44 pKa = 10.46 NKK46 pKa = 9.85 ALQAKK51 pKa = 9.71 VEE53 pKa = 4.19 FEE55 pKa = 4.31 KK56 pKa = 11.32 NGATRR61 pKa = 11.84 TAFTQFEE68 pKa = 5.0 TYY70 pKa = 10.49 SEE72 pKa = 4.9 LLDD75 pKa = 4.05 SWWEE79 pKa = 4.2 TYY81 pKa = 11.16 APTIKK86 pKa = 9.84 TNSQIAIKK94 pKa = 10.04 SQIEE98 pKa = 4.28 NYY100 pKa = 9.59 LRR102 pKa = 11.84 PAFGSYY108 pKa = 10.74 KK109 pKa = 10.11 LDD111 pKa = 3.41 KK112 pKa = 9.33 LTPAIIQQQVNRR124 pKa = 11.84 WARR127 pKa = 11.84 DD128 pKa = 3.33 YY129 pKa = 10.89 NQNDD133 pKa = 3.7 GGFKK137 pKa = 10.37 RR138 pKa = 11.84 YY139 pKa = 9.11 GQLHH143 pKa = 6.68 SYY145 pKa = 9.74 NKK147 pKa = 10.3 RR148 pKa = 11.84 ILQYY152 pKa = 10.7 GVSLQALKK160 pKa = 10.96 SNPAKK165 pKa = 10.48 NVLVPKK171 pKa = 10.6 VKK173 pKa = 9.61 TDD175 pKa = 3.11 KK176 pKa = 11.4 AKK178 pKa = 10.39 IKK180 pKa = 10.6 HH181 pKa = 5.38 FTDD184 pKa = 3.69 SEE186 pKa = 4.26 LKK188 pKa = 10.66 QFFEE192 pKa = 4.71 YY193 pKa = 10.73 LDD195 pKa = 4.04 SLDD198 pKa = 3.34 QKK200 pKa = 10.71 RR201 pKa = 11.84 FKK203 pKa = 11.24 NLFCVTLYY211 pKa = 11.07 KK212 pKa = 10.56 FLLATGCRR220 pKa = 11.84 INEE223 pKa = 3.93 ALALEE228 pKa = 4.42 WADD231 pKa = 3.18 IDD233 pKa = 4.27 LDD235 pKa = 3.83 NAVVHH240 pKa = 5.5 ITKK243 pKa = 9.22 TLNYY247 pKa = 8.97 RR248 pKa = 11.84 LEE250 pKa = 4.38 INSPKK255 pKa = 10.42 SKK257 pKa = 10.48 SSYY260 pKa = 10.15 RR261 pKa = 11.84 DD262 pKa = 2.72 IDD264 pKa = 3.47 IDD266 pKa = 3.86 PKK268 pKa = 9.31 TVSMMKK274 pKa = 9.83 QYY276 pKa = 11.1 KK277 pKa = 9.55 HH278 pKa = 6.31 RR279 pKa = 11.84 QTKK282 pKa = 7.88 EE283 pKa = 3.02 AWTLGRR289 pKa = 11.84 TEE291 pKa = 4.49 KK292 pKa = 10.94 VVFSDD297 pKa = 6.3 FIHH300 pKa = 7.08 EE301 pKa = 4.28 YY302 pKa = 9.74 PNNRR306 pKa = 11.84 TLQARR311 pKa = 11.84 LKK313 pKa = 10.14 IHH315 pKa = 6.86 FKK317 pKa = 9.9 QAQVPDD323 pKa = 3.32 IGFHH327 pKa = 5.86 GFRR330 pKa = 11.84 HH331 pKa = 4.87 THH333 pKa = 6.74 ASLLLNAGIPYY344 pKa = 10.01 KK345 pKa = 10.28 EE346 pKa = 3.98 LQHH349 pKa = 6.73 RR350 pKa = 11.84 LGHH353 pKa = 5.68 STLSMTMDD361 pKa = 4.17 TYY363 pKa = 11.68 SHH365 pKa = 7.14 LSKK368 pKa = 10.81 EE369 pKa = 4.43 SAKK372 pKa = 10.29 KK373 pKa = 10.0 AVSIFEE379 pKa = 4.14 TALNNIKK386 pKa = 10.62 SSS388 pKa = 3.38
Molecular weight: 44.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.017
IPC2_protein 9.151
IPC_protein 9.019
Toseland 9.853
ProMoST 9.516
Dawson 10.058
Bjellqvist 9.706
Wikipedia 10.218
Rodwell 10.57
Grimsley 10.131
Solomon 10.072
Lehninger 10.043
Nozaki 9.838
DTASelect 9.706
Thurlkill 9.911
EMBOSS 10.262
Sillero 9.97
Patrickios 9.897
IPC_peptide 10.072
IPC2_peptide 8.156
IPC2.peptide.svr19 8.096
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
1959
58
530
217.7
25.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.513 ± 0.761
0.408 ± 0.15
6.023 ± 0.446
7.759 ± 1.023
4.033 ± 0.579
4.594 ± 0.591
1.582 ± 0.381
7.606 ± 0.481
11.026 ± 0.457
10.158 ± 0.885
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.889 ± 0.325
6.228 ± 0.456
2.297 ± 0.191
4.084 ± 0.395
3.675 ± 0.519
6.942 ± 0.605
5.666 ± 0.808
5.054 ± 0.511
0.919 ± 0.191
4.543 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here