Escherichia phage 2725-N35

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Braunvirinae; Rtpvirus; unclassified Rtpvirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9SS90|A0A6B9SS90_9CAUD Uncharacterized protein OS=Escherichia phage 2725-N35 OX=2692738 PE=4 SV=1
MM1 pKa = 7.63KK2 pKa = 10.41VYY4 pKa = 10.83LLNAYY9 pKa = 8.33TDD11 pKa = 3.62YY12 pKa = 11.54DD13 pKa = 3.6GFEE16 pKa = 3.88ICGVFDD22 pKa = 3.81TEE24 pKa = 5.42EE25 pKa = 3.81LAEE28 pKa = 4.11LQKK31 pKa = 11.27NILIGIDD38 pKa = 3.12IEE40 pKa = 4.39RR41 pKa = 11.84GYY43 pKa = 11.31GPDD46 pKa = 3.18YY47 pKa = 11.11YY48 pKa = 10.81LIEE51 pKa = 4.37EE52 pKa = 4.52FDD54 pKa = 3.8VKK56 pKa = 11.02VAII59 pKa = 4.74

Molecular weight: 6.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9SSC0|A0A6B9SSC0_9CAUD Uncharacterized protein OS=Escherichia phage 2725-N35 OX=2692738 PE=4 SV=1
MM1 pKa = 6.8NTVDD5 pKa = 4.7KK6 pKa = 10.59RR7 pKa = 11.84TINGNNGTIRR17 pKa = 11.84TEE19 pKa = 4.03DD20 pKa = 2.82KK21 pKa = 10.5KK22 pKa = 10.72QRR24 pKa = 11.84KK25 pKa = 8.53RR26 pKa = 11.84PSGYY30 pKa = 10.06YY31 pKa = 8.95VLKK34 pKa = 10.9DD35 pKa = 3.51EE36 pKa = 4.52VRR38 pKa = 11.84AGLRR42 pKa = 11.84ARR44 pKa = 11.84LEE46 pKa = 3.84IVLDD50 pKa = 3.88FFGTKK55 pKa = 10.47ANIAKK60 pKa = 9.21QLKK63 pKa = 7.58VTPQAVEE70 pKa = 3.72EE71 pKa = 4.24WFKK74 pKa = 11.38RR75 pKa = 11.84GMISARR81 pKa = 11.84GAQLSHH87 pKa = 6.72NYY89 pKa = 9.66YY90 pKa = 10.0KK91 pKa = 10.9RR92 pKa = 11.84NGEE95 pKa = 3.9GFRR98 pKa = 11.84ATFCRR103 pKa = 11.84PDD105 pKa = 3.38LQFDD109 pKa = 4.02GNGRR113 pKa = 11.84PLTLRR118 pKa = 11.84CKK120 pKa = 9.49KK121 pKa = 9.98RR122 pKa = 11.84HH123 pKa = 4.86MLRR126 pKa = 11.84VVTEE130 pKa = 4.33AEE132 pKa = 4.14LATKK136 pKa = 9.16PEE138 pKa = 4.04CRR140 pKa = 11.84SWRR143 pKa = 11.84KK144 pKa = 9.37IKK146 pKa = 10.91LEE148 pKa = 3.88NQKK151 pKa = 10.55KK152 pKa = 9.56KK153 pKa = 9.65EE154 pKa = 4.02QNSTII159 pKa = 3.68

Molecular weight: 18.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13427

41

1128

181.4

20.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.595 ± 0.549

1.214 ± 0.159

6.01 ± 0.26

6.688 ± 0.381

3.985 ± 0.176

7.597 ± 0.316

1.281 ± 0.207

6.733 ± 0.191

6.517 ± 0.392

6.733 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.569 ± 0.243

5.191 ± 0.296

3.009 ± 0.244

3.88 ± 0.399

4.677 ± 0.225

7.001 ± 0.392

5.817 ± 0.376

7.433 ± 0.305

1.184 ± 0.103

3.888 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski