Ralstonia phage RP13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 264 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J4EGN9|A0A6J4EGN9_9CAUD Uncharacterized protein OS=Ralstonia phage RP13 OX=2743402 PE=4 SV=1
MM1 pKa = 7.57LSISNLGIVNDD12 pKa = 3.82VKK14 pKa = 11.02ALQLSSIYY22 pKa = 10.1QIQSGLDD29 pKa = 3.33SLDD32 pKa = 3.75IIAITLFGDD41 pKa = 3.37QNYY44 pKa = 7.95WKK46 pKa = 10.61YY47 pKa = 10.3LAYY50 pKa = 11.01YY51 pKa = 10.29NDD53 pKa = 3.28IEE55 pKa = 4.59NPFGLISQGYY65 pKa = 6.82TSIKK69 pKa = 10.18VFSKK73 pKa = 11.06SDD75 pKa = 3.09IDD77 pKa = 4.5LLINN81 pKa = 4.03

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J4EGK9|A0A6J4EGK9_9CAUD Uncharacterized protein OS=Ralstonia phage RP13 OX=2743402 PE=4 SV=1
MM1 pKa = 7.07NSKK4 pKa = 8.86YY5 pKa = 9.41QRR7 pKa = 11.84FFAHH11 pKa = 5.76VRR13 pKa = 11.84SGYY16 pKa = 7.99VTVVHH21 pKa = 6.54HH22 pKa = 6.15GVMMVDD28 pKa = 3.23VLVGTGANVSIKK40 pKa = 10.5HH41 pKa = 6.33LLTADD46 pKa = 4.47EE47 pKa = 4.8ISHH50 pKa = 6.77LGNRR54 pKa = 11.84IQQRR58 pKa = 11.84VKK60 pKa = 10.59AARR63 pKa = 11.84HH64 pKa = 4.57AA65 pKa = 4.02

Molecular weight: 7.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

264

0

264

50946

36

1059

193.0

21.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.215 ± 0.187

1.076 ± 0.058

6.401 ± 0.125

5.834 ± 0.191

3.735 ± 0.091

6.038 ± 0.169

2.232 ± 0.094

7.125 ± 0.148

6.475 ± 0.177

8.281 ± 0.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.683 ± 0.095

5.555 ± 0.138

3.806 ± 0.111

3.771 ± 0.107

4.328 ± 0.117

6.782 ± 0.17

6.393 ± 0.157

6.839 ± 0.152

1.229 ± 0.066

4.202 ± 0.112
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski