Nitrolancea hollandica Lb

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Thermomicrobia; Sphaerobacteridae; Sphaerobacterales; Sphaerobacterineae; Sphaerobacteraceae; Nitrolancea; Nitrolancea hollandica

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3969 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4EJR4|I4EJR4_9CHLR Putative ABC-type branched-chain amino acid transport systems periplasmic binding component OS=Nitrolancea hollandica Lb OX=1129897 GN=NITHO_4210003 PE=4 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84RR3 pKa = 11.84VLWAIGLTLGLLSSLLFAGTAMAAGLGSVGTLSGAVSGVTDD44 pKa = 3.9TVSSVADD51 pKa = 3.56PVTATVSDD59 pKa = 3.85VAGAISDD66 pKa = 3.85AGSNGAGNNVNSGNTTSAGLIAVNDD91 pKa = 4.09LVDD94 pKa = 3.85VSALNGGNGGDD105 pKa = 3.3AGLNVSSPDD114 pKa = 3.34VTVGGLVAVNDD125 pKa = 4.03AVDD128 pKa = 3.46VSLLNGGNGDD138 pKa = 3.76GSGAGNNTNTGNTTTAGLIAVNDD161 pKa = 4.14LADD164 pKa = 3.47VSALNGANANGSHH177 pKa = 6.65AGSNAGNNTNGDD189 pKa = 3.67NTTSAGLIAVNDD201 pKa = 4.33GVDD204 pKa = 3.15ASLLNGANANGSHH217 pKa = 6.2SEE219 pKa = 3.99ANAGNNTNSGNTTSAGLVAVNDD241 pKa = 5.5GIDD244 pKa = 3.16ASLLNGANANGNHH257 pKa = 6.49AEE259 pKa = 4.1ANAGNNANSGNVTSGGLAGINDD281 pKa = 5.43GIDD284 pKa = 2.9ASFGNGANANGSLSGANAGNNVNTGNTTSSGVAGVNDD321 pKa = 5.59GIDD324 pKa = 3.2AGLLNGGNANGKK336 pKa = 8.24HH337 pKa = 5.77AEE339 pKa = 4.16VNAGNNVNTGNTTTGGLVGANDD361 pKa = 5.53LIDD364 pKa = 3.58ATLINGANANGDD376 pKa = 3.88HH377 pKa = 6.29AHH379 pKa = 7.01ANAGNNVNAGNTTSGGLIGINDD401 pKa = 4.46LLGATALNGANANGEE416 pKa = 4.17GARR419 pKa = 11.84ANAGNNINSGNITSGGLIAINDD441 pKa = 4.31PLDD444 pKa = 3.93LSLLNGANADD454 pKa = 3.59GSVGNHH460 pKa = 5.92GCTQNCGGDD469 pKa = 3.38NGCEE473 pKa = 3.9HH474 pKa = 7.79DD475 pKa = 4.09CGGDD479 pKa = 3.21HH480 pKa = 7.36GCGHH484 pKa = 7.57DD485 pKa = 3.51CGGDD489 pKa = 3.16HH490 pKa = 7.23GCTHH494 pKa = 6.61NCGGDD499 pKa = 3.64HH500 pKa = 6.46EE501 pKa = 5.28CSSNCGGNHH510 pKa = 7.04GGCTSNCSGNDD521 pKa = 3.27GHH523 pKa = 7.28CIANCEE529 pKa = 4.16GTSDD533 pKa = 3.49GGKK536 pKa = 10.09NNHH539 pKa = 6.54EE540 pKa = 4.47GNDD543 pKa = 4.13PIDD546 pKa = 4.16GGNHH550 pKa = 6.47NGGDD554 pKa = 3.84DD555 pKa = 3.86PADD558 pKa = 3.9GGTGGDD564 pKa = 3.59GNGWANDD571 pKa = 3.52PTTTNSRR578 pKa = 11.84QPDD581 pKa = 3.88PVSAFLPLVYY591 pKa = 10.18TPDD594 pKa = 4.04DD595 pKa = 4.21PQPTMLLLGLSDD607 pKa = 4.4PPAIAPEE614 pKa = 4.15TGQPEE619 pKa = 4.2RR620 pKa = 11.84RR621 pKa = 11.84FFDD624 pKa = 3.62LALITLGLISLTSGTFVRR642 pKa = 11.84FRR644 pKa = 11.84ARR646 pKa = 11.84RR647 pKa = 11.84VSS649 pKa = 3.11

Molecular weight: 62.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4ECS4|I4ECS4_9CHLR Formamidopyrimidine-DNA glycosylase OS=Nitrolancea hollandica Lb OX=1129897 GN=mutM PE=3 SV=1
MM1 pKa = 7.37LVCSGGVVVALLALSSSATFSGKK24 pKa = 9.97RR25 pKa = 11.84STATRR30 pKa = 11.84STEE33 pKa = 3.81DD34 pKa = 3.17TAGGTTHH41 pKa = 7.36ALSSPAHH48 pKa = 6.34PAGNPPRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84DD58 pKa = 3.42LEE60 pKa = 4.23VTFRR64 pKa = 11.84RR65 pKa = 11.84LGLKK69 pKa = 9.56HH70 pKa = 6.34GRR72 pKa = 11.84IHH74 pKa = 7.38LPYY77 pKa = 10.29KK78 pKa = 10.32RR79 pKa = 11.84VLEE82 pKa = 4.23PAPATAMPPVLRR94 pKa = 11.84VSAQAPVPPIPMLGVSCGPRR114 pKa = 11.84MVMDD118 pKa = 4.75HH119 pKa = 7.14PPGALCYY126 pKa = 10.37

Molecular weight: 13.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3969

0

3969

962534

10

3045

242.5

26.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.803 ± 0.042

0.828 ± 0.014

5.434 ± 0.033

5.975 ± 0.045

3.343 ± 0.026

8.402 ± 0.042

2.225 ± 0.022

5.443 ± 0.034

2.356 ± 0.026

10.369 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.122 ± 0.02

2.512 ± 0.025

5.834 ± 0.032

3.584 ± 0.027

7.946 ± 0.05

5.561 ± 0.031

5.561 ± 0.037

7.686 ± 0.039

1.539 ± 0.019

2.476 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski