Acidothermus cellulolyticus (strain ATCC 43068 / 11B)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Acidothermales; Acidothermaceae; Acidothermus; Acidothermus cellulolyticus

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2157 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0LWA7|A0LWA7_ACIC1 50S ribosomal protein L25 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351607 GN=rplY PE=3 SV=1
MM1 pKa = 7.6TYY3 pKa = 10.0IIAQPCVDD11 pKa = 3.72VKK13 pKa = 11.25DD14 pKa = 3.94RR15 pKa = 11.84ACVDD19 pKa = 3.71EE20 pKa = 5.5CPVDD24 pKa = 4.52CIYY27 pKa = 10.86EE28 pKa = 4.25GQRR31 pKa = 11.84MLYY34 pKa = 9.05IHH36 pKa = 7.53PDD38 pKa = 3.09EE39 pKa = 5.5CVDD42 pKa = 4.38CGACEE47 pKa = 3.85PVCPVEE53 pKa = 4.7AIFYY57 pKa = 10.61EE58 pKa = 5.52DD59 pKa = 5.35DD60 pKa = 6.53LPDD63 pKa = 3.27QWKK66 pKa = 10.33DD67 pKa = 3.52YY68 pKa = 10.69YY69 pKa = 10.76KK70 pKa = 11.16VNVEE74 pKa = 3.97FFEE77 pKa = 5.32DD78 pKa = 3.46LGSPGGASKK87 pKa = 11.03VGVIDD92 pKa = 3.72HH93 pKa = 6.8DD94 pKa = 4.28HH95 pKa = 7.28PIVAALPPQGEE106 pKa = 4.31AGG108 pKa = 3.47

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0LRK2|A0LRK2_ACIC1 UPF0336 protein Acel_0288 OS=Acidothermus cellulolyticus (strain ATCC 43068 / 11B) OX=351607 GN=Acel_0288 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.72HH17 pKa = 5.74RR18 pKa = 11.84KK19 pKa = 7.77LLKK22 pKa = 8.07RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.81KK32 pKa = 9.63

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2157

0

2157

729829

32

1785

338.4

36.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.731 ± 0.076

0.851 ± 0.015

5.777 ± 0.043

5.134 ± 0.06

2.928 ± 0.029

8.768 ± 0.045

2.178 ± 0.023

4.197 ± 0.033

1.711 ± 0.033

10.05 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.623 ± 0.019

1.909 ± 0.032

6.176 ± 0.054

2.764 ± 0.032

8.499 ± 0.06

5.083 ± 0.059

5.914 ± 0.045

9.191 ± 0.054

1.388 ± 0.024

2.127 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski