Nonlabens sediminis
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2951 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A090PXP2|A0A090PXP2_9FLAO Uncharacterized protein OS=Nonlabens sediminis OX=319236 GN=JCM19294_2287 PE=4 SV=1
MM1 pKa = 7.2 VSCDD5 pKa = 3.79 EE6 pKa = 5.69 DD7 pKa = 3.67 PTLSNDD13 pKa = 3.4 QNQGDD18 pKa = 4.52 DD19 pKa = 5.09 DD20 pKa = 3.9 ITTIDD25 pKa = 3.68 DD26 pKa = 3.83 KK27 pKa = 11.79 VRR29 pKa = 11.84 LSSIQIAEE37 pKa = 4.11 NTASNIVTNIDD48 pKa = 2.79 IVYY51 pKa = 10.7 DD52 pKa = 3.65 NDD54 pKa = 3.41 NLISEE59 pKa = 4.73 IVFSGQQSKK68 pKa = 8.6 TYY70 pKa = 9.77 QFQYY74 pKa = 11.06 APNNQVINYY83 pKa = 7.26 TKK85 pKa = 10.11 IEE87 pKa = 4.06 NQSTSSYY94 pKa = 10.22 SIEE97 pKa = 3.84 YY98 pKa = 9.91 DD99 pKa = 3.4 EE100 pKa = 6.26 NIITSSNVSNPNQIQQFEE118 pKa = 4.16 IDD120 pKa = 3.26 NFNRR124 pKa = 11.84 VDD126 pKa = 4.15 EE127 pKa = 4.44 ILSRR131 pKa = 11.84 TTSSNGSVIQEE142 pKa = 3.85 NSEE145 pKa = 4.1 VFDD148 pKa = 3.74 INANFNIEE156 pKa = 4.28 RR157 pKa = 11.84 ITYY160 pKa = 10.13 LEE162 pKa = 4.16 NNNTTGFSDD171 pKa = 3.77 FTYY174 pKa = 10.53 EE175 pKa = 5.24 LNDD178 pKa = 4.02 NPFKK182 pKa = 11.22 DD183 pKa = 3.54 MNDD186 pKa = 3.67 IIRR189 pKa = 11.84 LLVFKK194 pKa = 10.58 DD195 pKa = 5.35 FIPSSRR201 pKa = 11.84 FLPLTQEE208 pKa = 4.46 EE209 pKa = 5.43 YY210 pKa = 9.36 DD211 pKa = 3.58 TSTGSIQLVQEE222 pKa = 4.19 MIYY225 pKa = 9.99 TYY227 pKa = 11.0 NLNSEE232 pKa = 4.29 GMPVSRR238 pKa = 11.84 EE239 pKa = 3.33 ISTTNNGISSTSYY252 pKa = 11.5 NLFSYY257 pKa = 10.51 LL258 pKa = 3.99
Molecular weight: 29.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.528
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.567
Grimsley 3.439
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.05
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.859
Patrickios 1.1
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.776
Protein with the highest isoelectric point:
>tr|A0A090Q4B2|A0A090Q4B2_9FLAO Sulf_transp domain-containing protein OS=Nonlabens sediminis OX=319236 GN=JCM19294_1556 PE=3 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.46 KK3 pKa = 9.83 LLKK6 pKa = 10.03 YY7 pKa = 10.27 SVYY10 pKa = 10.06 IYY12 pKa = 10.45 LIFAIVCLITAITEE26 pKa = 4.23 WNVEE30 pKa = 3.78 RR31 pKa = 11.84 SRR33 pKa = 11.84 AYY35 pKa = 11.3 LMLFMAAVALFMFFFRR51 pKa = 11.84 RR52 pKa = 11.84 RR53 pKa = 11.84 FIRR56 pKa = 11.84 RR57 pKa = 11.84 FDD59 pKa = 3.92 DD60 pKa = 3.47 RR61 pKa = 11.84 NKK63 pKa = 10.95
Molecular weight: 7.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.911
IPC_protein 10.672
Toseland 10.526
ProMoST 10.379
Dawson 10.701
Bjellqvist 10.423
Wikipedia 10.921
Rodwell 10.921
Grimsley 10.774
Solomon 10.774
Lehninger 10.745
Nozaki 10.511
DTASelect 10.423
Thurlkill 10.555
EMBOSS 10.935
Sillero 10.613
Patrickios 10.672
IPC_peptide 10.774
IPC2_peptide 9.443
IPC2.peptide.svr19 8.347
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2951
0
2951
886951
37
2417
300.6
33.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.462 ± 0.042
0.724 ± 0.015
6.027 ± 0.044
6.437 ± 0.047
4.892 ± 0.035
6.369 ± 0.045
1.856 ± 0.023
7.616 ± 0.042
6.978 ± 0.07
9.186 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.249 ± 0.031
6.044 ± 0.056
3.456 ± 0.029
3.87 ± 0.031
3.833 ± 0.032
6.575 ± 0.044
5.845 ± 0.054
6.379 ± 0.034
1.079 ± 0.017
4.123 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here