Avian paraavulavirus 3
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D5FGX3|D5FGX3_9MONO Phosphoprotein OS=Avian paraavulavirus 3 OX=2560327 PE=4 SV=1
MM1 pKa = 7.51 AGIFNTYY8 pKa = 8.9 EE9 pKa = 4.47 LFVKK13 pKa = 10.42 DD14 pKa = 3.59 QTCMHH19 pKa = 6.52 KK20 pKa = 10.31 RR21 pKa = 11.84 AASLISGGQLKK32 pKa = 11.04 SNIPVFITTRR42 pKa = 11.84 DD43 pKa = 3.64 DD44 pKa = 3.86 PAVRR48 pKa = 11.84 WNLVCFNLRR57 pKa = 11.84 LIVSEE62 pKa = 4.42 SSTSVIRR69 pKa = 11.84 QGAMISLLSVTASNMRR85 pKa = 11.84 ALAAIAGQTDD95 pKa = 3.12 EE96 pKa = 4.87 SMINIIEE103 pKa = 4.22 VVDD106 pKa = 4.23 FNGLEE111 pKa = 4.17 PQCDD115 pKa = 3.61 PRR117 pKa = 11.84 SGLDD121 pKa = 3.31 AQKK124 pKa = 10.97 QDD126 pKa = 3.37 MFKK129 pKa = 10.79 DD130 pKa = 3.46 IASDD134 pKa = 3.56 MPKK137 pKa = 10.18 VLGSGTPFQNVSAEE151 pKa = 4.35 TNNPEE156 pKa = 4.0 DD157 pKa = 2.71 THH159 pKa = 5.91 MFLRR163 pKa = 11.84 SAISVLTQIWILVAKK178 pKa = 10.57 AMTNIEE184 pKa = 4.21 GSHH187 pKa = 5.58 EE188 pKa = 4.02 ASDD191 pKa = 3.71 RR192 pKa = 11.84 RR193 pKa = 11.84 LAKK196 pKa = 9.15 YY197 pKa = 6.8 TQQNRR202 pKa = 11.84 IDD204 pKa = 3.84 RR205 pKa = 11.84 RR206 pKa = 11.84 FMLAQATRR214 pKa = 11.84 TACQQIIKK222 pKa = 10.37 DD223 pKa = 3.65 SLTIRR228 pKa = 11.84 RR229 pKa = 11.84 FLVTEE234 pKa = 4.05 LRR236 pKa = 11.84 KK237 pKa = 10.22 SRR239 pKa = 11.84 GALHH243 pKa = 6.61 SGSSYY248 pKa = 10.2 YY249 pKa = 11.61 AMVGDD254 pKa = 3.76 MQAYY258 pKa = 9.01 IFNAGLTPFLTTLRR272 pKa = 11.84 YY273 pKa = 10.01 GIGTKK278 pKa = 9.71 YY279 pKa = 9.83 HH280 pKa = 6.69 ALAISSLTGDD290 pKa = 3.69 LNKK293 pKa = 10.25 IKK295 pKa = 10.77 GLLTLYY301 pKa = 10.44 KK302 pKa = 10.41 EE303 pKa = 4.44 KK304 pKa = 10.97 GSDD307 pKa = 3.31 AGYY310 pKa = 8.59 MALLEE315 pKa = 5.34 DD316 pKa = 4.72 ADD318 pKa = 4.33 CMQFAPGNYY327 pKa = 9.65 ALLYY331 pKa = 9.13 SYY333 pKa = 11.68 AMGVASVHH341 pKa = 6.57 DD342 pKa = 4.01 EE343 pKa = 3.81 GMRR346 pKa = 11.84 NYY348 pKa = 10.34 QYY350 pKa = 11.66 ARR352 pKa = 11.84 RR353 pKa = 11.84 FLHH356 pKa = 6.34 KK357 pKa = 10.55 GMYY360 pKa = 9.57 QFGRR364 pKa = 11.84 DD365 pKa = 2.85 IATQHH370 pKa = 4.81 QHH372 pKa = 6.96 ALDD375 pKa = 3.76 EE376 pKa = 4.51 SLAQEE381 pKa = 4.08 MRR383 pKa = 11.84 ITEE386 pKa = 4.09 ADD388 pKa = 3.74 RR389 pKa = 11.84 ANLKK393 pKa = 10.76 VMMANIGEE401 pKa = 4.21 ASHH404 pKa = 6.71 YY405 pKa = 10.95 SDD407 pKa = 4.53 IPSAGPSGIPAFNDD421 pKa = 3.37 PPEE424 pKa = 4.14 EE425 pKa = 4.0 LFGEE429 pKa = 4.25 PSYY432 pKa = 11.23 RR433 pKa = 11.84 KK434 pKa = 9.91 LPEE437 pKa = 4.05 EE438 pKa = 4.08 PQVVEE443 pKa = 4.38 LQDD446 pKa = 4.46 RR447 pKa = 11.84 DD448 pKa = 4.56 DD449 pKa = 5.18 DD450 pKa = 4.2 EE451 pKa = 4.75 QDD453 pKa = 3.42 EE454 pKa = 4.6 YY455 pKa = 12.1 DD456 pKa = 3.37 MM457 pKa = 6.54
Molecular weight: 50.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.271
IPC2_protein 5.296
IPC_protein 5.283
Toseland 5.41
ProMoST 5.499
Dawson 5.372
Bjellqvist 5.461
Wikipedia 5.308
Rodwell 5.308
Grimsley 5.41
Solomon 5.372
Lehninger 5.347
Nozaki 5.55
DTASelect 5.741
Thurlkill 5.486
EMBOSS 5.474
Sillero 5.626
Patrickios 4.024
IPC_peptide 5.385
IPC2_peptide 5.626
IPC2.peptide.svr19 5.599
Protein with the highest isoelectric point:
>tr|D5FGX5|D5FGX5_9MONO Fusion glycoprotein F0 OS=Avian paraavulavirus 3 OX=2560327 GN=F PE=3 SV=1
MM1 pKa = 7.55 AAHH4 pKa = 7.22 PNHH7 pKa = 6.99 ANPSSSISLMHH18 pKa = 7.63 DD19 pKa = 3.5 DD20 pKa = 5.63 PSIQTQLLAFPLISEE35 pKa = 4.31 KK36 pKa = 10.54 TEE38 pKa = 3.77 TGTTKK43 pKa = 10.57 LQPQVRR49 pKa = 11.84 MQSFLSTDD57 pKa = 3.07 SQKK60 pKa = 10.89 YY61 pKa = 9.26 HH62 pKa = 6.38 LVFINTYY69 pKa = 10.34 GFIAEE74 pKa = 5.21 DD75 pKa = 4.69 FNCSPTNGFVPALFQPKK92 pKa = 10.21 SKK94 pKa = 10.29 VLSSAMVTLGAVPADD109 pKa = 3.67 TVLQDD114 pKa = 3.67 LQKK117 pKa = 11.21 DD118 pKa = 4.44 LIAMRR123 pKa = 11.84 FKK125 pKa = 10.78 VRR127 pKa = 11.84 KK128 pKa = 8.73 SASAKK133 pKa = 10.0 EE134 pKa = 4.36 LILFSTDD141 pKa = 3.52 NIPATLTGSSVWKK154 pKa = 9.85 NRR156 pKa = 11.84 GVIADD161 pKa = 3.74 TATSVKK167 pKa = 10.31 APGRR171 pKa = 11.84 ISCDD175 pKa = 3.25 AVCSYY180 pKa = 11.01 CITFISFCFFHH191 pKa = 7.8 SSALFKK197 pKa = 10.99 VPKK200 pKa = 9.56 PLLNFEE206 pKa = 4.2 TAVAYY211 pKa = 10.62 SLVLQVEE218 pKa = 4.53 LEE220 pKa = 4.08 FPNIKK225 pKa = 9.15 DD226 pKa = 3.71 TLHH229 pKa = 6.46 EE230 pKa = 4.86 KK231 pKa = 10.12 YY232 pKa = 10.72 LKK234 pKa = 11.01 NKK236 pKa = 9.43 DD237 pKa = 3.87 SKK239 pKa = 10.11 WYY241 pKa = 8.35 CTIDD245 pKa = 3.09 IHH247 pKa = 8.41 IGNLLKK253 pKa = 10.05 RR254 pKa = 11.84 TAKK257 pKa = 9.2 QRR259 pKa = 11.84 RR260 pKa = 11.84 RR261 pKa = 11.84 TPSEE265 pKa = 3.25 ITQKK269 pKa = 9.29 VRR271 pKa = 11.84 RR272 pKa = 11.84 MGFRR276 pKa = 11.84 IGLYY280 pKa = 9.89 DD281 pKa = 3.61 LWGPTIVVEE290 pKa = 4.17 LTGSSSKK297 pKa = 10.74 SLQGFFSSEE306 pKa = 3.47 RR307 pKa = 11.84 LACHH311 pKa = 7.22 PISQYY316 pKa = 10.74 NPHH319 pKa = 6.29 VGQLIWAHH327 pKa = 6.2 DD328 pKa = 3.73 VSITGCHH335 pKa = 5.71 MIISEE340 pKa = 4.31 LEE342 pKa = 4.02 KK343 pKa = 10.93 KK344 pKa = 9.98 KK345 pKa = 10.84 ALAMADD351 pKa = 3.78 LTVSDD356 pKa = 4.43 AVAINTTIKK365 pKa = 10.42 EE366 pKa = 4.2 LVPFRR371 pKa = 11.84 LFRR374 pKa = 11.84 KK375 pKa = 9.62
Molecular weight: 41.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.626
IPC2_protein 8.434
IPC_protein 8.302
Toseland 8.99
ProMoST 8.96
Dawson 9.311
Bjellqvist 9.224
Wikipedia 9.399
Rodwell 9.575
Grimsley 9.238
Solomon 9.385
Lehninger 9.37
Nozaki 9.458
DTASelect 9.077
Thurlkill 9.253
EMBOSS 9.487
Sillero 9.414
Patrickios 4.927
IPC_peptide 9.385
IPC2_peptide 7.951
IPC2.peptide.svr19 7.781
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4535
375
2198
755.8
84.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.086 ± 0.626
2.426 ± 0.337
5.006 ± 0.36
4.3 ± 0.295
3.462 ± 0.557
4.939 ± 0.311
2.492 ± 0.262
8.093 ± 0.872
4.19 ± 0.525
11.51 ± 1.231
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.073 ± 0.367
5.05 ± 0.311
4.851 ± 0.585
4.564 ± 0.371
4.917 ± 0.351
9.46 ± 0.663
7.1 ± 0.492
5.204 ± 0.265
0.794 ± 0.169
3.44 ± 0.461
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here