Yata virus
Average proteome isoelectric point is 6.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A096ZGU9|A0A096ZGU9_9RHAB GDP polyribonucleotidyltransferase OS=Yata virus OX=1272960 GN=L PE=4 SV=1
MM1 pKa = 7.68 ALLLSGVLTKK11 pKa = 10.67 SDD13 pKa = 3.74 FQTDD17 pKa = 3.12 NQLFDD22 pKa = 4.07 FVEE25 pKa = 4.91 KK26 pKa = 10.23 IVEE29 pKa = 4.16 EE30 pKa = 4.3 TTRR33 pKa = 11.84 LAIKK37 pKa = 10.67 NSIWWSGLQFTQIEE51 pKa = 4.37 RR52 pKa = 11.84 EE53 pKa = 4.4 VMVGMNWLVYY63 pKa = 9.97 IDD65 pKa = 4.58 NEE67 pKa = 3.78 EE68 pKa = 4.15 SDD70 pKa = 3.85 RR71 pKa = 11.84 VDD73 pKa = 3.07 YY74 pKa = 10.97 SFWFGVPDD82 pKa = 3.67 SVGWRR87 pKa = 11.84 MFRR90 pKa = 11.84 NPNTRR95 pKa = 11.84 CIASNILEE103 pKa = 4.2 NRR105 pKa = 11.84 QSYY108 pKa = 10.36 CFIQYY113 pKa = 9.58 FSQQ116 pKa = 3.45
Molecular weight: 13.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.616
IPC2_protein 4.647
IPC_protein 4.495
Toseland 4.329
ProMoST 4.596
Dawson 4.444
Bjellqvist 4.596
Wikipedia 4.317
Rodwell 4.329
Grimsley 4.24
Solomon 4.431
Lehninger 4.38
Nozaki 4.546
DTASelect 4.698
Thurlkill 4.342
EMBOSS 4.329
Sillero 4.596
Patrickios 3.859
IPC_peptide 4.431
IPC2_peptide 4.584
IPC2.peptide.svr19 4.563
Protein with the highest isoelectric point:
>tr|A0A096ZGV7|A0A096ZGV7_9RHAB Isoform of A0A096ZGU4 Alpha2x protein OS=Yata virus OX=1272960 GN=alpha2 PE=4 SV=1
MM1 pKa = 7.07 IARR4 pKa = 11.84 WRR6 pKa = 11.84 KK7 pKa = 9.69 DD8 pKa = 3.23 RR9 pKa = 11.84 ADD11 pKa = 3.59 KK12 pKa = 10.9 AKK14 pKa = 10.35 KK15 pKa = 9.98 DD16 pKa = 3.73 SPPEE20 pKa = 3.97 YY21 pKa = 10.17 SSSSSLWMSTAPAYY35 pKa = 10.35 DD36 pKa = 3.72 GSFGPIFHH44 pKa = 6.72 NPKK47 pKa = 10.2 PEE49 pKa = 3.87 PTKK52 pKa = 9.36 QAFMIEE58 pKa = 4.28 CSLEE62 pKa = 4.28 VISKK66 pKa = 9.99 KK67 pKa = 8.58 QVEE70 pKa = 4.8 GVKK73 pKa = 10.77 GMLKK77 pKa = 9.97 ILDD80 pKa = 4.33 HH81 pKa = 7.12 LVDD84 pKa = 4.61 NYY86 pKa = 11.39 DD87 pKa = 2.94 GSYY90 pKa = 8.62 WGKK93 pKa = 8.99 PLIVMMYY100 pKa = 10.03 LVLGTHH106 pKa = 6.06 MEE108 pKa = 4.21 SKK110 pKa = 10.62 SRR112 pKa = 11.84 IGIDD116 pKa = 2.58 SWIYY120 pKa = 10.06 QRR122 pKa = 11.84 SLSEE126 pKa = 3.57 AVYY129 pKa = 10.17 IITEE133 pKa = 4.48 TPVSMTSSGLTYY145 pKa = 11.06 NNYY148 pKa = 8.29 TQTTYY153 pKa = 10.87 LGEE156 pKa = 4.39 PASVTYY162 pKa = 10.41 SFKK165 pKa = 10.3 ATPTKK170 pKa = 10.25 RR171 pKa = 11.84 YY172 pKa = 8.76 SRR174 pKa = 11.84 PIIGAYY180 pKa = 9.31 KK181 pKa = 10.39 LPLANGLPPPEE192 pKa = 4.8 LNDD195 pKa = 3.1 VLRR198 pKa = 11.84 YY199 pKa = 9.92 YY200 pKa = 11.4 GLTTRR205 pKa = 11.84 IQDD208 pKa = 3.48 NGEE211 pKa = 4.01 SSVEE215 pKa = 3.9 FLPP218 pKa = 5.13
Molecular weight: 24.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.66
IPC2_protein 7.819
IPC_protein 7.995
Toseland 8.287
ProMoST 8.346
Dawson 8.595
Bjellqvist 8.448
Wikipedia 8.697
Rodwell 8.624
Grimsley 8.346
Solomon 8.829
Lehninger 8.785
Nozaki 8.404
DTASelect 8.448
Thurlkill 8.492
EMBOSS 8.756
Sillero 8.668
Patrickios 4.012
IPC_peptide 8.814
IPC2_peptide 7.337
IPC2.peptide.svr19 7.556
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10
1
11
4743
53
2117
431.2
49.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.563 ± 0.472
1.982 ± 0.284
5.988 ± 0.312
6.156 ± 0.413
3.9 ± 0.312
5.882 ± 0.328
2.277 ± 0.186
8.202 ± 0.402
7.147 ± 0.266
9.994 ± 0.47
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.635 ± 0.31
5.376 ± 0.295
4.132 ± 0.425
2.867 ± 0.13
4.786 ± 0.349
7.506 ± 0.442
5.925 ± 0.171
5.355 ± 0.226
1.855 ± 0.168
4.47 ± 0.484
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here