Amino acid dipepetide frequency for Mycobacterium phage BeesKnees

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.793AlaAla: 12.793 ± 1.248
0.624AlaCys: 0.624 ± 0.178
7.177AlaAsp: 7.177 ± 0.627
5.866AlaGlu: 5.866 ± 0.743
3.994AlaPhe: 3.994 ± 0.504
8.05AlaGly: 8.05 ± 0.862
1.373AlaHis: 1.373 ± 0.339
3.994AlaIle: 3.994 ± 0.574
4.493AlaLys: 4.493 ± 0.651
8.799AlaLeu: 8.799 ± 0.813
2.683AlaMet: 2.683 ± 0.46
2.309AlaAsn: 2.309 ± 0.294
4.805AlaPro: 4.805 ± 0.676
3.058AlaGln: 3.058 ± 0.463
5.866AlaArg: 5.866 ± 0.535
5.679AlaSer: 5.679 ± 0.706
5.617AlaThr: 5.617 ± 0.657
8.799AlaVal: 8.799 ± 0.836
1.685AlaTrp: 1.685 ± 0.325
2.621AlaTyr: 2.621 ± 0.354
0.0AlaXaa: 0.0 ± 0.0
Cys
0.749CysAla: 0.749 ± 0.214
0.0CysCys: 0.0 ± 0.0
0.437CysAsp: 0.437 ± 0.164
0.624CysGlu: 0.624 ± 0.162
0.062CysPhe: 0.062 ± 0.059
0.749CysGly: 0.749 ± 0.288
0.187CysHis: 0.187 ± 0.105
0.25CysIle: 0.25 ± 0.114
0.187CysLys: 0.187 ± 0.119
0.374CysLeu: 0.374 ± 0.189
0.25CysMet: 0.25 ± 0.124
0.25CysAsn: 0.25 ± 0.118
0.312CysPro: 0.312 ± 0.151
0.187CysGln: 0.187 ± 0.113
0.562CysArg: 0.562 ± 0.185
0.437CysSer: 0.437 ± 0.166
0.25CysThr: 0.25 ± 0.122
0.187CysVal: 0.187 ± 0.11
0.25CysTrp: 0.25 ± 0.114
0.125CysTyr: 0.125 ± 0.087
0.0CysXaa: 0.0 ± 0.0
Asp
6.428AspAla: 6.428 ± 0.574
0.437AspCys: 0.437 ± 0.156
4.556AspAsp: 4.556 ± 0.602
3.432AspGlu: 3.432 ± 0.494
2.309AspPhe: 2.309 ± 0.336
6.553AspGly: 6.553 ± 0.711
1.311AspHis: 1.311 ± 0.288
2.871AspIle: 2.871 ± 0.508
2.871AspLys: 2.871 ± 0.42
7.114AspLeu: 7.114 ± 0.749
1.373AspMet: 1.373 ± 0.308
1.935AspAsn: 1.935 ± 0.387
5.242AspPro: 5.242 ± 0.555
1.685AspGln: 1.685 ± 0.372
3.37AspArg: 3.37 ± 0.412
2.933AspSer: 2.933 ± 0.487
4.056AspThr: 4.056 ± 0.431
4.618AspVal: 4.618 ± 0.492
1.872AspTrp: 1.872 ± 0.373
1.81AspTyr: 1.81 ± 0.312
0.0AspXaa: 0.0 ± 0.0
Glu
5.991GluAla: 5.991 ± 0.721
0.25GluCys: 0.25 ± 0.144
5.242GluAsp: 5.242 ± 0.501
4.743GluGlu: 4.743 ± 0.575
2.309GluPhe: 2.309 ± 0.394
3.682GluGly: 3.682 ± 0.562
1.623GluHis: 1.623 ± 0.371
3.37GluIle: 3.37 ± 0.507
2.559GluLys: 2.559 ± 0.388
6.365GluLeu: 6.365 ± 0.687
1.56GluMet: 1.56 ± 0.282
1.623GluAsn: 1.623 ± 0.382
2.496GluPro: 2.496 ± 0.439
2.496GluGln: 2.496 ± 0.401
3.682GluArg: 3.682 ± 0.503
3.37GluSer: 3.37 ± 0.339
3.744GluThr: 3.744 ± 0.498
5.242GluVal: 5.242 ± 0.525
1.81GluTrp: 1.81 ± 0.365
2.309GluTyr: 2.309 ± 0.468
0.0GluXaa: 0.0 ± 0.0
Phe
2.746PheAla: 2.746 ± 0.428
0.312PheCys: 0.312 ± 0.182
3.058PheAsp: 3.058 ± 0.374
2.309PheGlu: 2.309 ± 0.348
0.562PhePhe: 0.562 ± 0.177
3.744PheGly: 3.744 ± 0.513
0.874PheHis: 0.874 ± 0.282
1.248PheIle: 1.248 ± 0.277
1.186PheLys: 1.186 ± 0.325
2.496PheLeu: 2.496 ± 0.403
0.749PheMet: 0.749 ± 0.196
1.248PheAsn: 1.248 ± 0.306
1.747PhePro: 1.747 ± 0.313
0.874PheGln: 0.874 ± 0.256
2.059PheArg: 2.059 ± 0.313
1.81PheSer: 1.81 ± 0.317
2.309PheThr: 2.309 ± 0.42
2.122PheVal: 2.122 ± 0.412
0.499PheTrp: 0.499 ± 0.157
0.999PheTyr: 0.999 ± 0.238
0.0PheXaa: 0.0 ± 0.0
Gly
7.302GlyAla: 7.302 ± 0.835
0.562GlyCys: 0.562 ± 0.174
5.492GlyAsp: 5.492 ± 0.556
4.306GlyGlu: 4.306 ± 0.495
2.996GlyPhe: 2.996 ± 0.447
9.735GlyGly: 9.735 ± 1.71
1.81GlyHis: 1.81 ± 0.392
4.431GlyIle: 4.431 ± 0.868
3.432GlyLys: 3.432 ± 0.536
7.614GlyLeu: 7.614 ± 0.947
1.872GlyMet: 1.872 ± 0.375
3.557GlyAsn: 3.557 ± 0.402
4.056GlyPro: 4.056 ± 0.574
2.247GlyGln: 2.247 ± 0.309
4.805GlyArg: 4.805 ± 0.648
6.615GlySer: 6.615 ± 0.783
5.117GlyThr: 5.117 ± 0.582
5.367GlyVal: 5.367 ± 0.482
2.683GlyTrp: 2.683 ± 0.37
2.933GlyTyr: 2.933 ± 0.436
0.0GlyXaa: 0.0 ± 0.0
His
1.685HisAla: 1.685 ± 0.345
0.25HisCys: 0.25 ± 0.158
1.248HisAsp: 1.248 ± 0.264
1.498HisGlu: 1.498 ± 0.307
0.686HisPhe: 0.686 ± 0.195
1.498HisGly: 1.498 ± 0.336
0.999HisHis: 0.999 ± 0.292
0.811HisIle: 0.811 ± 0.207
1.123HisLys: 1.123 ± 0.294
1.373HisLeu: 1.373 ± 0.332
0.125HisMet: 0.125 ± 0.089
0.187HisAsn: 0.187 ± 0.1
1.623HisPro: 1.623 ± 0.256
0.811HisGln: 0.811 ± 0.229
1.498HisArg: 1.498 ± 0.303
0.749HisSer: 0.749 ± 0.197
0.936HisThr: 0.936 ± 0.246
1.81HisVal: 1.81 ± 0.33
0.562HisTrp: 0.562 ± 0.164
0.874HisTyr: 0.874 ± 0.257
0.0HisXaa: 0.0 ± 0.0
Ile
6.053IleAla: 6.053 ± 0.757
0.25IleCys: 0.25 ± 0.102
3.432IleAsp: 3.432 ± 0.329
3.744IleGlu: 3.744 ± 0.451
1.123IlePhe: 1.123 ± 0.283
3.994IleGly: 3.994 ± 0.481
0.874IleHis: 0.874 ± 0.207
1.872IleIle: 1.872 ± 0.33
2.184IleLys: 2.184 ± 0.375
3.245IleLeu: 3.245 ± 0.35
0.686IleMet: 0.686 ± 0.187
1.872IleAsn: 1.872 ± 0.317
2.871IlePro: 2.871 ± 0.343
1.373IleGln: 1.373 ± 0.356
3.557IleArg: 3.557 ± 0.435
3.12IleSer: 3.12 ± 0.488
3.682IleThr: 3.682 ± 0.519
2.683IleVal: 2.683 ± 0.499
0.686IleTrp: 0.686 ± 0.182
1.872IleTyr: 1.872 ± 0.274
0.0IleXaa: 0.0 ± 0.0
Lys
4.119LysAla: 4.119 ± 0.606
0.374LysCys: 0.374 ± 0.16
2.871LysAsp: 2.871 ± 0.484
1.872LysGlu: 1.872 ± 0.369
1.435LysPhe: 1.435 ± 0.274
2.559LysGly: 2.559 ± 0.395
1.123LysHis: 1.123 ± 0.292
2.309LysIle: 2.309 ± 0.472
1.747LysLys: 1.747 ± 0.419
3.495LysLeu: 3.495 ± 0.505
0.999LysMet: 0.999 ± 0.218
1.311LysAsn: 1.311 ± 0.257
2.559LysPro: 2.559 ± 0.439
1.81LysGln: 1.81 ± 0.384
2.871LysArg: 2.871 ± 0.449
2.371LysSer: 2.371 ± 0.343
2.434LysThr: 2.434 ± 0.373
3.183LysVal: 3.183 ± 0.503
0.936LysTrp: 0.936 ± 0.217
0.936LysTyr: 0.936 ± 0.268
0.0LysXaa: 0.0 ± 0.0
Leu
9.299LeuAla: 9.299 ± 0.878
0.312LeuCys: 0.312 ± 0.138
6.677LeuAsp: 6.677 ± 0.623
5.367LeuGlu: 5.367 ± 0.646
2.371LeuPhe: 2.371 ± 0.394
6.927LeuGly: 6.927 ± 0.699
1.56LeuHis: 1.56 ± 0.292
4.556LeuIle: 4.556 ± 0.448
4.056LeuLys: 4.056 ± 0.525
5.929LeuLeu: 5.929 ± 0.66
1.81LeuMet: 1.81 ± 0.262
2.996LeuAsn: 2.996 ± 0.413
5.679LeuPro: 5.679 ± 0.555
2.933LeuGln: 2.933 ± 0.527
6.116LeuArg: 6.116 ± 0.607
5.242LeuSer: 5.242 ± 0.514
5.866LeuThr: 5.866 ± 0.429
4.93LeuVal: 4.93 ± 0.662
1.061LeuTrp: 1.061 ± 0.28
2.059LeuTyr: 2.059 ± 0.377
0.0LeuXaa: 0.0 ± 0.0
Met
2.309MetAla: 2.309 ± 0.398
0.0MetCys: 0.0 ± 0.0
0.874MetAsp: 0.874 ± 0.191
1.623MetGlu: 1.623 ± 0.356
0.686MetPhe: 0.686 ± 0.195
1.685MetGly: 1.685 ± 0.338
0.437MetHis: 0.437 ± 0.151
0.874MetIle: 0.874 ± 0.26
0.999MetLys: 0.999 ± 0.261
1.123MetLeu: 1.123 ± 0.233
0.187MetMet: 0.187 ± 0.113
0.999MetAsn: 0.999 ± 0.212
1.248MetPro: 1.248 ± 0.267
0.499MetGln: 0.499 ± 0.152
1.186MetArg: 1.186 ± 0.321
1.997MetSer: 1.997 ± 0.333
2.309MetThr: 2.309 ± 0.349
1.123MetVal: 1.123 ± 0.302
0.187MetTrp: 0.187 ± 0.094
0.374MetTyr: 0.374 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
3.62AsnAla: 3.62 ± 0.457
0.062AsnCys: 0.062 ± 0.073
1.997AsnAsp: 1.997 ± 0.399
1.935AsnGlu: 1.935 ± 0.4
0.999AsnPhe: 0.999 ± 0.286
3.37AsnGly: 3.37 ± 0.463
0.811AsnHis: 0.811 ± 0.174
1.623AsnIle: 1.623 ± 0.352
0.499AsnLys: 0.499 ± 0.182
2.559AsnLeu: 2.559 ± 0.362
0.437AsnMet: 0.437 ± 0.166
1.123AsnAsn: 1.123 ± 0.383
2.933AsnPro: 2.933 ± 0.376
1.186AsnGln: 1.186 ± 0.245
1.373AsnArg: 1.373 ± 0.317
1.623AsnSer: 1.623 ± 0.296
1.81AsnThr: 1.81 ± 0.287
2.184AsnVal: 2.184 ± 0.364
0.811AsnTrp: 0.811 ± 0.189
1.311AsnTyr: 1.311 ± 0.259
0.0AsnXaa: 0.0 ± 0.0
Pro
5.117ProAla: 5.117 ± 0.621
0.624ProCys: 0.624 ± 0.198
4.68ProAsp: 4.68 ± 0.531
4.431ProGlu: 4.431 ± 0.571
2.184ProPhe: 2.184 ± 0.355
4.743ProGly: 4.743 ± 0.596
0.874ProHis: 0.874 ± 0.213
2.621ProIle: 2.621 ± 0.395
2.122ProLys: 2.122 ± 0.282
4.68ProLeu: 4.68 ± 0.493
0.936ProMet: 0.936 ± 0.262
1.872ProAsn: 1.872 ± 0.317
2.808ProPro: 2.808 ± 0.437
1.435ProGln: 1.435 ± 0.314
2.933ProArg: 2.933 ± 0.432
3.744ProSer: 3.744 ± 0.481
3.557ProThr: 3.557 ± 0.524
3.557ProVal: 3.557 ± 0.457
0.874ProTrp: 0.874 ± 0.32
1.623ProTyr: 1.623 ± 0.366
0.0ProXaa: 0.0 ± 0.0
Gln
3.058GlnAla: 3.058 ± 0.448
0.187GlnCys: 0.187 ± 0.118
1.186GlnAsp: 1.186 ± 0.351
1.747GlnGlu: 1.747 ± 0.336
1.311GlnPhe: 1.311 ± 0.277
2.559GlnGly: 2.559 ± 0.346
0.562GlnHis: 0.562 ± 0.171
2.559GlnIle: 2.559 ± 0.535
1.061GlnLys: 1.061 ± 0.251
3.932GlnLeu: 3.932 ± 0.491
0.999GlnMet: 0.999 ± 0.294
0.374GlnAsn: 0.374 ± 0.135
1.935GlnPro: 1.935 ± 0.325
1.81GlnGln: 1.81 ± 0.432
1.81GlnArg: 1.81 ± 0.397
1.997GlnSer: 1.997 ± 0.345
1.623GlnThr: 1.623 ± 0.291
2.309GlnVal: 2.309 ± 0.346
0.686GlnTrp: 0.686 ± 0.193
0.499GlnTyr: 0.499 ± 0.158
0.0GlnXaa: 0.0 ± 0.0
Arg
5.741ArgAla: 5.741 ± 0.695
0.624ArgCys: 0.624 ± 0.221
2.871ArgAsp: 2.871 ± 0.391
4.431ArgGlu: 4.431 ± 0.659
1.935ArgPhe: 1.935 ± 0.357
5.305ArgGly: 5.305 ± 0.723
0.999ArgHis: 0.999 ± 0.232
2.996ArgIle: 2.996 ± 0.587
3.308ArgLys: 3.308 ± 0.545
5.804ArgLeu: 5.804 ± 0.821
1.872ArgMet: 1.872 ± 0.383
2.184ArgAsn: 2.184 ± 0.45
2.434ArgPro: 2.434 ± 0.411
1.935ArgGln: 1.935 ± 0.351
5.117ArgArg: 5.117 ± 0.614
3.557ArgSer: 3.557 ± 0.563
3.058ArgThr: 3.058 ± 0.532
4.993ArgVal: 4.993 ± 0.623
1.435ArgTrp: 1.435 ± 0.288
1.935ArgTyr: 1.935 ± 0.33
0.0ArgXaa: 0.0 ± 0.0
Ser
5.741SerAla: 5.741 ± 0.639
0.686SerCys: 0.686 ± 0.239
2.933SerAsp: 2.933 ± 0.401
3.744SerGlu: 3.744 ± 0.449
2.184SerPhe: 2.184 ± 0.456
6.74SerGly: 6.74 ± 0.817
1.435SerHis: 1.435 ± 0.312
2.933SerIle: 2.933 ± 0.447
2.434SerLys: 2.434 ± 0.387
4.868SerLeu: 4.868 ± 0.467
1.685SerMet: 1.685 ± 0.292
2.122SerAsn: 2.122 ± 0.424
2.746SerPro: 2.746 ± 0.425
1.872SerGln: 1.872 ± 0.28
3.308SerArg: 3.308 ± 0.433
3.557SerSer: 3.557 ± 0.644
2.996SerThr: 2.996 ± 0.398
4.119SerVal: 4.119 ± 0.557
1.373SerTrp: 1.373 ± 0.335
1.623SerTyr: 1.623 ± 0.327
0.0SerXaa: 0.0 ± 0.0
Thr
6.677ThrAla: 6.677 ± 0.954
0.25ThrCys: 0.25 ± 0.128
4.368ThrAsp: 4.368 ± 0.587
3.994ThrGlu: 3.994 ± 0.492
2.059ThrPhe: 2.059 ± 0.358
6.615ThrGly: 6.615 ± 0.639
0.936ThrHis: 0.936 ± 0.3
3.058ThrIle: 3.058 ± 0.532
2.434ThrLys: 2.434 ± 0.398
6.178ThrLeu: 6.178 ± 0.601
0.686ThrMet: 0.686 ± 0.174
1.747ThrAsn: 1.747 ± 0.309
3.557ThrPro: 3.557 ± 0.46
1.623ThrGln: 1.623 ± 0.308
3.37ThrArg: 3.37 ± 0.594
3.12ThrSer: 3.12 ± 0.558
4.181ThrThr: 4.181 ± 0.531
4.993ThrVal: 4.993 ± 0.585
1.061ThrTrp: 1.061 ± 0.269
1.872ThrTyr: 1.872 ± 0.393
0.0ThrXaa: 0.0 ± 0.0
Val
6.553ValAla: 6.553 ± 0.8
0.312ValCys: 0.312 ± 0.143
5.367ValAsp: 5.367 ± 0.475
5.18ValGlu: 5.18 ± 0.587
2.122ValPhe: 2.122 ± 0.345
4.493ValGly: 4.493 ± 0.608
1.435ValHis: 1.435 ± 0.265
3.682ValIle: 3.682 ± 0.51
3.058ValLys: 3.058 ± 0.483
5.367ValLeu: 5.367 ± 0.617
0.811ValMet: 0.811 ± 0.265
2.496ValAsn: 2.496 ± 0.379
3.744ValPro: 3.744 ± 0.412
2.059ValGln: 2.059 ± 0.386
5.492ValArg: 5.492 ± 0.717
4.868ValSer: 4.868 ± 0.513
5.367ValThr: 5.367 ± 0.633
5.055ValVal: 5.055 ± 0.709
1.373ValTrp: 1.373 ± 0.275
2.184ValTyr: 2.184 ± 0.428
0.0ValXaa: 0.0 ± 0.0
Trp
1.685TrpAla: 1.685 ± 0.297
0.125TrpCys: 0.125 ± 0.085
1.123TrpAsp: 1.123 ± 0.272
1.311TrpGlu: 1.311 ± 0.276
1.061TrpPhe: 1.061 ± 0.262
1.498TrpGly: 1.498 ± 0.276
0.499TrpHis: 0.499 ± 0.174
1.435TrpIle: 1.435 ± 0.305
0.437TrpLys: 0.437 ± 0.254
1.872TrpLeu: 1.872 ± 0.337
0.437TrpMet: 0.437 ± 0.17
0.624TrpAsn: 0.624 ± 0.183
0.936TrpPro: 0.936 ± 0.271
1.186TrpGln: 1.186 ± 0.238
1.123TrpArg: 1.123 ± 0.29
0.811TrpSer: 0.811 ± 0.228
1.685TrpThr: 1.685 ± 0.353
1.935TrpVal: 1.935 ± 0.339
0.499TrpTrp: 0.499 ± 0.191
0.374TrpTyr: 0.374 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.621TyrAla: 2.621 ± 0.403
0.187TyrCys: 0.187 ± 0.127
1.186TyrAsp: 1.186 ± 0.295
2.309TyrGlu: 2.309 ± 0.346
0.562TyrPhe: 0.562 ± 0.168
2.434TyrGly: 2.434 ± 0.424
0.624TyrHis: 0.624 ± 0.201
1.685TyrIle: 1.685 ± 0.324
1.123TyrLys: 1.123 ± 0.303
2.559TyrLeu: 2.559 ± 0.419
0.499TyrMet: 0.499 ± 0.157
1.435TyrAsn: 1.435 ± 0.337
1.685TyrPro: 1.685 ± 0.362
1.123TyrGln: 1.123 ± 0.315
2.496TyrArg: 2.496 ± 0.382
1.498TyrSer: 1.498 ± 0.283
2.184TyrThr: 2.184 ± 0.366
1.81TyrVal: 1.81 ± 0.34
0.312TyrTrp: 0.312 ± 0.143
0.562TyrTyr: 0.562 ± 0.161
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (16025 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski