Amino acid dipepetide frequency for Pseudomonas phage Njord

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.697AlaAla: 9.697 ± 1.171
0.425AlaCys: 0.425 ± 0.166
5.875AlaAsp: 5.875 ± 0.698
6.512AlaGlu: 6.512 ± 0.802
3.114AlaPhe: 3.114 ± 0.484
7.857AlaGly: 7.857 ± 1.411
1.982AlaHis: 1.982 ± 0.388
4.105AlaIle: 4.105 ± 0.589
5.167AlaLys: 5.167 ± 0.622
7.078AlaLeu: 7.078 ± 0.93
3.327AlaMet: 3.327 ± 0.502
4.601AlaAsn: 4.601 ± 0.663
3.468AlaPro: 3.468 ± 0.617
4.388AlaGln: 4.388 ± 0.697
4.601AlaArg: 4.601 ± 0.941
4.53AlaSer: 4.53 ± 1.016
6.158AlaThr: 6.158 ± 0.792
7.007AlaVal: 7.007 ± 0.703
1.203AlaTrp: 1.203 ± 0.304
3.044AlaTyr: 3.044 ± 0.47
0.0AlaXaa: 0.0 ± 0.0
Cys
0.425CysAla: 0.425 ± 0.192
0.212CysCys: 0.212 ± 0.122
0.92CysAsp: 0.92 ± 0.267
0.566CysGlu: 0.566 ± 0.233
0.425CysPhe: 0.425 ± 0.184
0.849CysGly: 0.849 ± 0.265
0.142CysHis: 0.142 ± 0.096
0.849CysIle: 0.849 ± 0.334
0.637CysLys: 0.637 ± 0.243
0.779CysLeu: 0.779 ± 0.26
0.283CysMet: 0.283 ± 0.13
0.779CysAsn: 0.779 ± 0.213
0.425CysPro: 0.425 ± 0.188
0.425CysGln: 0.425 ± 0.191
0.495CysArg: 0.495 ± 0.218
0.354CysSer: 0.354 ± 0.139
0.354CysThr: 0.354 ± 0.198
0.566CysVal: 0.566 ± 0.206
0.0CysTrp: 0.0 ± 0.0
0.283CysTyr: 0.283 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
6.795AspAla: 6.795 ± 0.734
0.495AspCys: 0.495 ± 0.22
3.327AspAsp: 3.327 ± 0.401
3.964AspGlu: 3.964 ± 0.604
2.831AspPhe: 2.831 ± 0.485
6.087AspGly: 6.087 ± 0.847
1.203AspHis: 1.203 ± 0.395
2.76AspIle: 2.76 ± 0.521
4.601AspLys: 4.601 ± 0.736
4.742AspLeu: 4.742 ± 0.521
1.628AspMet: 1.628 ± 0.29
1.982AspAsn: 1.982 ± 0.413
3.398AspPro: 3.398 ± 0.615
2.619AspGln: 2.619 ± 0.397
3.327AspArg: 3.327 ± 0.466
3.468AspSer: 3.468 ± 0.383
3.893AspThr: 3.893 ± 0.545
3.822AspVal: 3.822 ± 0.646
1.133AspTrp: 1.133 ± 0.337
2.336AspTyr: 2.336 ± 0.404
0.0AspXaa: 0.0 ± 0.0
Glu
7.078GluAla: 7.078 ± 0.907
0.637GluCys: 0.637 ± 0.251
3.327GluAsp: 3.327 ± 0.352
4.459GluGlu: 4.459 ± 0.762
2.548GluPhe: 2.548 ± 0.425
4.813GluGly: 4.813 ± 0.623
1.274GluHis: 1.274 ± 0.293
2.194GluIle: 2.194 ± 0.33
3.185GluLys: 3.185 ± 0.418
6.512GluLeu: 6.512 ± 0.748
1.557GluMet: 1.557 ± 0.278
1.628GluAsn: 1.628 ± 0.281
2.194GluPro: 2.194 ± 0.407
2.194GluGln: 2.194 ± 0.432
3.539GluArg: 3.539 ± 0.754
3.044GluSer: 3.044 ± 0.451
2.69GluThr: 2.69 ± 0.536
4.813GluVal: 4.813 ± 0.664
0.92GluTrp: 0.92 ± 0.315
2.477GluTyr: 2.477 ± 0.443
0.0GluXaa: 0.0 ± 0.0
Phe
3.468PheAla: 3.468 ± 0.644
0.354PheCys: 0.354 ± 0.189
3.61PheAsp: 3.61 ± 0.602
1.84PheGlu: 1.84 ± 0.298
1.557PhePhe: 1.557 ± 0.381
2.123PheGly: 2.123 ± 0.334
0.779PheHis: 0.779 ± 0.252
2.194PheIle: 2.194 ± 0.384
1.628PheLys: 1.628 ± 0.295
2.831PheLeu: 2.831 ± 0.485
0.991PheMet: 0.991 ± 0.317
2.123PheAsn: 2.123 ± 0.416
1.699PhePro: 1.699 ± 0.429
1.416PheGln: 1.416 ± 0.397
2.336PheArg: 2.336 ± 0.378
2.477PheSer: 2.477 ± 0.424
2.902PheThr: 2.902 ± 0.659
2.548PheVal: 2.548 ± 0.673
0.566PheTrp: 0.566 ± 0.195
1.274PheTyr: 1.274 ± 0.39
0.0PheXaa: 0.0 ± 0.0
Gly
6.37GlyAla: 6.37 ± 0.67
0.708GlyCys: 0.708 ± 0.288
5.025GlyAsp: 5.025 ± 0.736
4.601GlyGlu: 4.601 ± 0.712
3.398GlyPhe: 3.398 ± 0.497
6.441GlyGly: 6.441 ± 0.798
1.982GlyHis: 1.982 ± 0.6
3.468GlyIle: 3.468 ± 0.449
6.37GlyLys: 6.37 ± 0.655
5.592GlyLeu: 5.592 ± 0.742
1.699GlyMet: 1.699 ± 0.333
3.539GlyAsn: 3.539 ± 0.432
1.416GlyPro: 1.416 ± 0.305
3.114GlyGln: 3.114 ± 0.399
4.105GlyArg: 4.105 ± 0.39
4.742GlySer: 4.742 ± 0.352
5.521GlyThr: 5.521 ± 0.713
5.521GlyVal: 5.521 ± 0.547
1.345GlyTrp: 1.345 ± 0.287
2.76GlyTyr: 2.76 ± 0.4
0.0GlyXaa: 0.0 ± 0.0
His
1.628HisAla: 1.628 ± 0.323
0.283HisCys: 0.283 ± 0.138
1.486HisAsp: 1.486 ± 0.36
0.991HisGlu: 0.991 ± 0.273
0.92HisPhe: 0.92 ± 0.232
1.77HisGly: 1.77 ± 0.352
0.495HisHis: 0.495 ± 0.212
0.991HisIle: 0.991 ± 0.232
1.486HisLys: 1.486 ± 0.348
2.619HisLeu: 2.619 ± 0.412
0.849HisMet: 0.849 ± 0.202
0.566HisAsn: 0.566 ± 0.208
1.557HisPro: 1.557 ± 0.416
1.274HisGln: 1.274 ± 0.296
1.133HisArg: 1.133 ± 0.237
1.274HisSer: 1.274 ± 0.316
0.849HisThr: 0.849 ± 0.253
1.062HisVal: 1.062 ± 0.283
0.708HisTrp: 0.708 ± 0.191
0.92HisTyr: 0.92 ± 0.282
0.0HisXaa: 0.0 ± 0.0
Ile
4.813IleAla: 4.813 ± 0.509
0.142IleCys: 0.142 ± 0.118
4.176IleAsp: 4.176 ± 0.534
3.185IleGlu: 3.185 ± 0.447
1.699IlePhe: 1.699 ± 0.423
3.893IleGly: 3.893 ± 0.645
1.345IleHis: 1.345 ± 0.256
2.69IleIle: 2.69 ± 0.613
2.548IleLys: 2.548 ± 0.324
1.982IleLeu: 1.982 ± 0.398
0.991IleMet: 0.991 ± 0.3
2.053IleAsn: 2.053 ± 0.464
2.831IlePro: 2.831 ± 0.502
2.053IleGln: 2.053 ± 0.483
2.973IleArg: 2.973 ± 0.347
2.407IleSer: 2.407 ± 0.332
2.902IleThr: 2.902 ± 0.469
2.902IleVal: 2.902 ± 0.469
0.92IleTrp: 0.92 ± 0.37
1.416IleTyr: 1.416 ± 0.31
0.0IleXaa: 0.0 ± 0.0
Lys
4.53LysAla: 4.53 ± 0.819
0.637LysCys: 0.637 ± 0.212
3.964LysAsp: 3.964 ± 0.732
4.105LysGlu: 4.105 ± 0.594
1.982LysPhe: 1.982 ± 0.352
4.388LysGly: 4.388 ± 0.581
0.991LysHis: 0.991 ± 0.217
1.77LysIle: 1.77 ± 0.306
2.619LysLys: 2.619 ± 0.458
5.875LysLeu: 5.875 ± 0.666
1.416LysMet: 1.416 ± 0.377
1.486LysAsn: 1.486 ± 0.349
2.407LysPro: 2.407 ± 0.516
2.477LysGln: 2.477 ± 0.608
3.61LysArg: 3.61 ± 0.474
2.336LysSer: 2.336 ± 0.333
3.185LysThr: 3.185 ± 0.413
4.176LysVal: 4.176 ± 0.467
1.062LysTrp: 1.062 ± 0.25
2.265LysTyr: 2.265 ± 0.399
0.0LysXaa: 0.0 ± 0.0
Leu
7.432LeuAla: 7.432 ± 0.879
0.92LeuCys: 0.92 ± 0.309
5.946LeuAsp: 5.946 ± 0.681
5.025LeuGlu: 5.025 ± 0.44
2.265LeuPhe: 2.265 ± 0.409
6.3LeuGly: 6.3 ± 0.649
1.77LeuHis: 1.77 ± 0.38
4.459LeuIle: 4.459 ± 0.628
3.398LeuLys: 3.398 ± 0.505
6.441LeuLeu: 6.441 ± 0.953
2.69LeuMet: 2.69 ± 0.462
3.964LeuAsn: 3.964 ± 0.635
3.893LeuPro: 3.893 ± 0.392
2.619LeuGln: 2.619 ± 0.467
4.742LeuArg: 4.742 ± 0.592
5.309LeuSer: 5.309 ± 0.722
6.3LeuThr: 6.3 ± 0.596
5.096LeuVal: 5.096 ± 0.692
1.062LeuTrp: 1.062 ± 0.304
2.194LeuTyr: 2.194 ± 0.422
0.0LeuXaa: 0.0 ± 0.0
Met
3.539MetAla: 3.539 ± 0.591
0.071MetCys: 0.071 ± 0.065
1.486MetAsp: 1.486 ± 0.327
1.982MetGlu: 1.982 ± 0.487
1.274MetPhe: 1.274 ± 0.265
1.911MetGly: 1.911 ± 0.361
0.637MetHis: 0.637 ± 0.23
1.203MetIle: 1.203 ± 0.313
1.982MetLys: 1.982 ± 0.471
2.619MetLeu: 2.619 ± 0.414
1.133MetMet: 1.133 ± 0.327
1.203MetAsn: 1.203 ± 0.361
1.416MetPro: 1.416 ± 0.343
1.062MetGln: 1.062 ± 0.336
2.265MetArg: 2.265 ± 0.441
1.982MetSer: 1.982 ± 0.367
2.265MetThr: 2.265 ± 0.335
1.77MetVal: 1.77 ± 0.435
0.212MetTrp: 0.212 ± 0.135
0.849MetTyr: 0.849 ± 0.241
0.0MetXaa: 0.0 ± 0.0
Asn
3.468AsnAla: 3.468 ± 0.395
0.071AsnCys: 0.071 ± 0.07
2.265AsnAsp: 2.265 ± 0.464
2.76AsnGlu: 2.76 ± 0.39
1.486AsnPhe: 1.486 ± 0.383
3.893AsnGly: 3.893 ± 0.405
1.203AsnHis: 1.203 ± 0.241
2.477AsnIle: 2.477 ± 0.495
2.265AsnLys: 2.265 ± 0.498
3.114AsnLeu: 3.114 ± 0.441
1.345AsnMet: 1.345 ± 0.273
1.486AsnAsn: 1.486 ± 0.281
2.831AsnPro: 2.831 ± 0.421
1.628AsnGln: 1.628 ± 0.324
3.044AsnArg: 3.044 ± 0.441
2.194AsnSer: 2.194 ± 0.483
2.902AsnThr: 2.902 ± 0.504
2.477AsnVal: 2.477 ± 0.394
0.495AsnTrp: 0.495 ± 0.202
1.628AsnTyr: 1.628 ± 0.411
0.0AsnXaa: 0.0 ± 0.0
Pro
3.822ProAla: 3.822 ± 0.714
0.495ProCys: 0.495 ± 0.232
2.69ProAsp: 2.69 ± 0.453
3.185ProGlu: 3.185 ± 0.536
1.77ProPhe: 1.77 ± 0.357
1.84ProGly: 1.84 ± 0.199
1.416ProHis: 1.416 ± 0.298
1.982ProIle: 1.982 ± 0.453
2.123ProLys: 2.123 ± 0.391
3.751ProLeu: 3.751 ± 0.529
1.203ProMet: 1.203 ± 0.222
2.407ProAsn: 2.407 ± 0.477
1.911ProPro: 1.911 ± 0.427
2.053ProGln: 2.053 ± 0.326
1.486ProArg: 1.486 ± 0.298
1.911ProSer: 1.911 ± 0.363
2.831ProThr: 2.831 ± 0.351
3.893ProVal: 3.893 ± 0.573
0.849ProTrp: 0.849 ± 0.223
1.486ProTyr: 1.486 ± 0.296
0.0ProXaa: 0.0 ± 0.0
Gln
5.167GlnAla: 5.167 ± 0.803
0.425GlnCys: 0.425 ± 0.206
2.194GlnAsp: 2.194 ± 0.473
1.982GlnGlu: 1.982 ± 0.296
2.548GlnPhe: 2.548 ± 0.509
2.831GlnGly: 2.831 ± 0.329
0.849GlnHis: 0.849 ± 0.238
1.911GlnIle: 1.911 ± 0.348
2.407GlnLys: 2.407 ± 0.587
3.822GlnLeu: 3.822 ± 0.554
1.699GlnMet: 1.699 ± 0.414
1.982GlnAsn: 1.982 ± 0.372
1.628GlnPro: 1.628 ± 0.325
3.044GlnGln: 3.044 ± 0.555
2.123GlnArg: 2.123 ± 0.394
2.265GlnSer: 2.265 ± 0.525
2.407GlnThr: 2.407 ± 0.34
2.69GlnVal: 2.69 ± 0.456
0.425GlnTrp: 0.425 ± 0.169
1.203GlnTyr: 1.203 ± 0.312
0.0GlnXaa: 0.0 ± 0.0
Arg
5.025ArgAla: 5.025 ± 0.867
0.92ArgCys: 0.92 ± 0.287
3.327ArgAsp: 3.327 ± 0.382
3.114ArgGlu: 3.114 ± 0.675
2.123ArgPhe: 2.123 ± 0.427
3.751ArgGly: 3.751 ± 0.395
1.416ArgHis: 1.416 ± 0.288
3.185ArgIle: 3.185 ± 0.426
2.548ArgLys: 2.548 ± 0.521
4.813ArgLeu: 4.813 ± 0.503
2.477ArgMet: 2.477 ± 0.394
2.548ArgAsn: 2.548 ± 0.384
1.699ArgPro: 1.699 ± 0.359
2.194ArgGln: 2.194 ± 0.477
2.477ArgArg: 2.477 ± 0.467
2.973ArgSer: 2.973 ± 0.362
3.256ArgThr: 3.256 ± 0.74
4.53ArgVal: 4.53 ± 0.676
0.637ArgTrp: 0.637 ± 0.201
1.699ArgTyr: 1.699 ± 0.396
0.0ArgXaa: 0.0 ± 0.0
Ser
4.884SerAla: 4.884 ± 0.739
0.425SerCys: 0.425 ± 0.148
3.468SerAsp: 3.468 ± 0.393
2.619SerGlu: 2.619 ± 0.715
2.336SerPhe: 2.336 ± 0.424
3.751SerGly: 3.751 ± 0.405
1.062SerHis: 1.062 ± 0.335
3.114SerIle: 3.114 ± 0.533
2.548SerLys: 2.548 ± 0.368
4.955SerLeu: 4.955 ± 0.618
1.982SerMet: 1.982 ± 0.553
1.84SerAsn: 1.84 ± 0.338
1.77SerPro: 1.77 ± 0.372
2.336SerGln: 2.336 ± 0.518
3.114SerArg: 3.114 ± 0.48
2.407SerSer: 2.407 ± 0.405
2.902SerThr: 2.902 ± 0.482
3.256SerVal: 3.256 ± 0.531
0.92SerTrp: 0.92 ± 0.264
2.336SerTyr: 2.336 ± 0.421
0.0SerXaa: 0.0 ± 0.0
Thr
5.025ThrAla: 5.025 ± 0.719
0.566ThrCys: 0.566 ± 0.205
3.256ThrAsp: 3.256 ± 0.396
3.256ThrGlu: 3.256 ± 0.468
2.902ThrPhe: 2.902 ± 0.619
6.37ThrGly: 6.37 ± 0.602
1.84ThrHis: 1.84 ± 0.339
3.185ThrIle: 3.185 ± 0.475
3.185ThrLys: 3.185 ± 0.574
5.309ThrLeu: 5.309 ± 0.67
2.477ThrMet: 2.477 ± 0.466
2.902ThrAsn: 2.902 ± 0.481
3.539ThrPro: 3.539 ± 0.41
3.185ThrGln: 3.185 ± 0.533
2.619ThrArg: 2.619 ± 0.399
2.477ThrSer: 2.477 ± 0.427
3.539ThrThr: 3.539 ± 0.716
3.822ThrVal: 3.822 ± 0.56
0.495ThrTrp: 0.495 ± 0.205
1.77ThrTyr: 1.77 ± 0.388
0.0ThrXaa: 0.0 ± 0.0
Val
6.724ValAla: 6.724 ± 0.827
0.991ValCys: 0.991 ± 0.252
4.884ValAsp: 4.884 ± 0.591
4.318ValGlu: 4.318 ± 0.516
2.265ValPhe: 2.265 ± 0.455
4.884ValGly: 4.884 ± 0.667
1.628ValHis: 1.628 ± 0.427
3.256ValIle: 3.256 ± 0.479
4.247ValLys: 4.247 ± 0.533
4.884ValLeu: 4.884 ± 0.668
1.84ValMet: 1.84 ± 0.385
3.185ValAsn: 3.185 ± 0.565
2.619ValPro: 2.619 ± 0.45
3.468ValGln: 3.468 ± 0.609
3.539ValArg: 3.539 ± 0.524
2.831ValSer: 2.831 ± 0.458
3.822ValThr: 3.822 ± 0.537
6.087ValVal: 6.087 ± 0.743
1.133ValTrp: 1.133 ± 0.279
2.194ValTyr: 2.194 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
1.486TrpAla: 1.486 ± 0.311
0.425TrpCys: 0.425 ± 0.182
0.991TrpAsp: 0.991 ± 0.236
1.133TrpGlu: 1.133 ± 0.265
0.495TrpPhe: 0.495 ± 0.189
1.133TrpGly: 1.133 ± 0.21
0.283TrpHis: 0.283 ± 0.118
0.708TrpIle: 0.708 ± 0.221
0.566TrpLys: 0.566 ± 0.181
1.203TrpLeu: 1.203 ± 0.265
0.283TrpMet: 0.283 ± 0.119
0.495TrpAsn: 0.495 ± 0.188
0.849TrpPro: 0.849 ± 0.266
0.566TrpGln: 0.566 ± 0.153
0.991TrpArg: 0.991 ± 0.241
0.779TrpSer: 0.779 ± 0.25
0.566TrpThr: 0.566 ± 0.195
0.991TrpVal: 0.991 ± 0.252
0.283TrpTrp: 0.283 ± 0.139
0.708TrpTyr: 0.708 ± 0.299
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.76TyrAla: 2.76 ± 0.412
0.566TyrCys: 0.566 ± 0.21
2.194TyrAsp: 2.194 ± 0.403
1.557TyrGlu: 1.557 ± 0.397
0.779TyrPhe: 0.779 ± 0.257
2.831TyrGly: 2.831 ± 0.404
0.566TyrHis: 0.566 ± 0.173
1.416TyrIle: 1.416 ± 0.288
1.77TyrLys: 1.77 ± 0.496
2.902TyrLeu: 2.902 ± 0.357
0.92TyrMet: 0.92 ± 0.266
2.265TyrAsn: 2.265 ± 0.366
1.557TyrPro: 1.557 ± 0.45
1.486TyrGln: 1.486 ± 0.32
2.194TyrArg: 2.194 ± 0.396
2.265TyrSer: 2.265 ± 0.508
2.477TyrThr: 2.477 ± 0.387
1.77TyrVal: 1.77 ± 0.375
0.566TyrTrp: 0.566 ± 0.22
0.637TyrTyr: 0.637 ± 0.212
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (14129 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski