Treponema azotonutricium (strain ATCC BAA-888 / DSM 13862 / ZAS-9)
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3457 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F5Y8N8|F5Y8N8_TREAZ Undecaprenyl-diphosphatase OS=Treponema azotonutricium (strain ATCC BAA-888 / DSM 13862 / ZAS-9) OX=545695 GN=uppP PE=3 SV=1
MM1 pKa = 7.17 KK2 pKa = 10.21 HH3 pKa = 6.67 LIIALLLSALLFAACSGKK21 pKa = 10.23 KK22 pKa = 8.98 EE23 pKa = 3.74 AARR26 pKa = 11.84 NSDD29 pKa = 2.66 ASEE32 pKa = 4.01 AVLGDD37 pKa = 3.33 VDD39 pKa = 4.26 EE40 pKa = 5.58 DD41 pKa = 4.03 LVSAALFAIYY51 pKa = 9.44 AQKK54 pKa = 9.3 GTPEE58 pKa = 3.72 WNALDD63 pKa = 3.55 SLGVHH68 pKa = 7.02 TIATDD73 pKa = 4.2 FSTPGAGGAVRR84 pKa = 11.84 VQLLQDD90 pKa = 4.11 LSSFMLAAVTFNDD103 pKa = 3.88 EE104 pKa = 5.49 DD105 pKa = 4.0 EE106 pKa = 5.21 DD107 pKa = 4.92 FYY109 pKa = 11.12 PSYY112 pKa = 11.63 VFFDD116 pKa = 3.68 IPGKK120 pKa = 10.69 AGDD123 pKa = 3.89 VYY125 pKa = 11.07 EE126 pKa = 4.56 FDD128 pKa = 5.38 AIFTEE133 pKa = 5.52 GIPLGSITAVRR144 pKa = 11.84 SDD146 pKa = 3.04 NRR148 pKa = 11.84 GNGDD152 pKa = 3.66 PYY154 pKa = 8.32 TTYY157 pKa = 11.26 YY158 pKa = 10.1 LQEE161 pKa = 4.31 SGFDD165 pKa = 3.61 GLPNGLVVYY174 pKa = 10.6 NEE176 pKa = 4.29 EE177 pKa = 4.35 FNSQEE182 pKa = 4.45 DD183 pKa = 3.61 EE184 pKa = 4.23 WEE186 pKa = 4.34 EE187 pKa = 3.81 PSAPPDD193 pKa = 3.0 QDD195 pKa = 4.34 AIYY198 pKa = 10.91 DD199 pKa = 4.06 EE200 pKa = 5.71 LLDD203 pKa = 4.91 NQDD206 pKa = 2.57 AWFAGLKK213 pKa = 8.53 ATSPFGSYY221 pKa = 10.39 DD222 pKa = 3.53 RR223 pKa = 11.84 NIFSGGDD230 pKa = 3.29 FYY232 pKa = 11.66 LGVGITLDD240 pKa = 3.38 IDD242 pKa = 3.84 GDD244 pKa = 4.56 GIFEE248 pKa = 4.42 LWFIATNQDD257 pKa = 2.58 RR258 pKa = 11.84 SEE260 pKa = 4.19 QYY262 pKa = 9.12 TFLYY266 pKa = 9.73 TLVEE270 pKa = 4.17 GKK272 pKa = 9.07 PLCLLSEE279 pKa = 4.27 LTLTYY284 pKa = 10.98 EE285 pKa = 4.41 DD286 pKa = 5.38 LSDD289 pKa = 3.82 NLLGFIDD296 pKa = 3.9 VVYY299 pKa = 10.55 DD300 pKa = 3.73 YY301 pKa = 9.03 DD302 pKa = 3.84 TLTQRR307 pKa = 11.84 IVFVRR312 pKa = 11.84 GNIRR316 pKa = 11.84 GFPGWSRR323 pKa = 11.84 RR324 pKa = 11.84 DD325 pKa = 3.08 VYY327 pKa = 11.26 YY328 pKa = 10.39 RR329 pKa = 11.84 YY330 pKa = 10.52 SRR332 pKa = 11.84 GKK334 pKa = 9.7 LDD336 pKa = 4.8 KK337 pKa = 10.87 IADD340 pKa = 4.17 LLSLNGSRR348 pKa = 11.84 NEE350 pKa = 3.8 LEE352 pKa = 3.83 AAEE355 pKa = 4.54 FLTGPEE361 pKa = 4.5 FYY363 pKa = 10.13 TGNGDD368 pKa = 4.13 DD369 pKa = 5.41 DD370 pKa = 3.59 ITVFFINGKK379 pKa = 9.53 AATADD384 pKa = 4.11 DD385 pKa = 3.79 YY386 pKa = 11.89 TLIVDD391 pKa = 4.23 SYY393 pKa = 9.98 VGSVLSRR400 pKa = 11.84 YY401 pKa = 8.16 YY402 pKa = 11.46 ANN404 pKa = 3.96
Molecular weight: 44.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.859
IPC_protein 3.884
Toseland 3.656
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.706
Grimsley 3.567
Solomon 3.872
Lehninger 3.821
Nozaki 3.973
DTASelect 4.228
Thurlkill 3.706
EMBOSS 3.808
Sillero 3.999
Patrickios 1.901
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|F5YBK1|F5YBK1_TREAZ Putative TPR domain protein OS=Treponema azotonutricium (strain ATCC BAA-888 / DSM 13862 / ZAS-9) OX=545695 GN=TREAZ_0576 PE=4 SV=1
MM1 pKa = 7.52 LGSSMGRR8 pKa = 11.84 PSLFSWGRR16 pKa = 11.84 MAFRR20 pKa = 11.84 LLTNPAGGKK29 pKa = 9.0 SGKK32 pKa = 9.69 GGKK35 pKa = 8.48 VWMRR39 pKa = 11.84 QIMRR43 pKa = 11.84 LMWTVV48 pKa = 2.74
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.404
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.061
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3457
0
3457
1156691
37
4075
334.6
37.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.075 ± 0.053
1.044 ± 0.017
5.421 ± 0.03
6.593 ± 0.043
4.624 ± 0.035
7.78 ± 0.048
1.587 ± 0.016
7.234 ± 0.039
6.09 ± 0.037
9.644 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.383 ± 0.021
4.146 ± 0.037
4.359 ± 0.032
2.817 ± 0.023
5.003 ± 0.036
6.472 ± 0.041
5.032 ± 0.046
6.103 ± 0.034
1.147 ± 0.014
3.444 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here