Treponema azotonutricium (strain ATCC BAA-888 / DSM 13862 / ZAS-9)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema; Treponema azotonutricium

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3457 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|F5Y8N8|F5Y8N8_TREAZ Undecaprenyl-diphosphatase OS=Treponema azotonutricium (strain ATCC BAA-888 / DSM 13862 / ZAS-9) OX=545695 GN=uppP PE=3 SV=1
MM1 pKa = 7.17KK2 pKa = 10.21HH3 pKa = 6.67LIIALLLSALLFAACSGKK21 pKa = 10.23KK22 pKa = 8.98EE23 pKa = 3.74AARR26 pKa = 11.84NSDD29 pKa = 2.66ASEE32 pKa = 4.01AVLGDD37 pKa = 3.33VDD39 pKa = 4.26EE40 pKa = 5.58DD41 pKa = 4.03LVSAALFAIYY51 pKa = 9.44AQKK54 pKa = 9.3GTPEE58 pKa = 3.72WNALDD63 pKa = 3.55SLGVHH68 pKa = 7.02TIATDD73 pKa = 4.2FSTPGAGGAVRR84 pKa = 11.84VQLLQDD90 pKa = 4.11LSSFMLAAVTFNDD103 pKa = 3.88EE104 pKa = 5.49DD105 pKa = 4.0EE106 pKa = 5.21DD107 pKa = 4.92FYY109 pKa = 11.12PSYY112 pKa = 11.63VFFDD116 pKa = 3.68IPGKK120 pKa = 10.69AGDD123 pKa = 3.89VYY125 pKa = 11.07EE126 pKa = 4.56FDD128 pKa = 5.38AIFTEE133 pKa = 5.52GIPLGSITAVRR144 pKa = 11.84SDD146 pKa = 3.04NRR148 pKa = 11.84GNGDD152 pKa = 3.66PYY154 pKa = 8.32TTYY157 pKa = 11.26YY158 pKa = 10.1LQEE161 pKa = 4.31SGFDD165 pKa = 3.61GLPNGLVVYY174 pKa = 10.6NEE176 pKa = 4.29EE177 pKa = 4.35FNSQEE182 pKa = 4.45DD183 pKa = 3.61EE184 pKa = 4.23WEE186 pKa = 4.34EE187 pKa = 3.81PSAPPDD193 pKa = 3.0QDD195 pKa = 4.34AIYY198 pKa = 10.91DD199 pKa = 4.06EE200 pKa = 5.71LLDD203 pKa = 4.91NQDD206 pKa = 2.57AWFAGLKK213 pKa = 8.53ATSPFGSYY221 pKa = 10.39DD222 pKa = 3.53RR223 pKa = 11.84NIFSGGDD230 pKa = 3.29FYY232 pKa = 11.66LGVGITLDD240 pKa = 3.38IDD242 pKa = 3.84GDD244 pKa = 4.56GIFEE248 pKa = 4.42LWFIATNQDD257 pKa = 2.58RR258 pKa = 11.84SEE260 pKa = 4.19QYY262 pKa = 9.12TFLYY266 pKa = 9.73TLVEE270 pKa = 4.17GKK272 pKa = 9.07PLCLLSEE279 pKa = 4.27LTLTYY284 pKa = 10.98EE285 pKa = 4.41DD286 pKa = 5.38LSDD289 pKa = 3.82NLLGFIDD296 pKa = 3.9VVYY299 pKa = 10.55DD300 pKa = 3.73YY301 pKa = 9.03DD302 pKa = 3.84TLTQRR307 pKa = 11.84IVFVRR312 pKa = 11.84GNIRR316 pKa = 11.84GFPGWSRR323 pKa = 11.84RR324 pKa = 11.84DD325 pKa = 3.08VYY327 pKa = 11.26YY328 pKa = 10.39RR329 pKa = 11.84YY330 pKa = 10.52SRR332 pKa = 11.84GKK334 pKa = 9.7LDD336 pKa = 4.8KK337 pKa = 10.87IADD340 pKa = 4.17LLSLNGSRR348 pKa = 11.84NEE350 pKa = 3.8LEE352 pKa = 3.83AAEE355 pKa = 4.54FLTGPEE361 pKa = 4.5FYY363 pKa = 10.13TGNGDD368 pKa = 4.13DD369 pKa = 5.41DD370 pKa = 3.59ITVFFINGKK379 pKa = 9.53AATADD384 pKa = 4.11DD385 pKa = 3.79YY386 pKa = 11.89TLIVDD391 pKa = 4.23SYY393 pKa = 9.98VGSVLSRR400 pKa = 11.84YY401 pKa = 8.16YY402 pKa = 11.46ANN404 pKa = 3.96

Molecular weight:
44.69 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|F5YBK1|F5YBK1_TREAZ Putative TPR domain protein OS=Treponema azotonutricium (strain ATCC BAA-888 / DSM 13862 / ZAS-9) OX=545695 GN=TREAZ_0576 PE=4 SV=1
MM1 pKa = 7.52LGSSMGRR8 pKa = 11.84PSLFSWGRR16 pKa = 11.84MAFRR20 pKa = 11.84LLTNPAGGKK29 pKa = 9.0SGKK32 pKa = 9.69GGKK35 pKa = 8.48VWMRR39 pKa = 11.84QIMRR43 pKa = 11.84LMWTVV48 pKa = 2.74

Molecular weight:
5.37 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3457

0

3457

1156691

37

4075

334.6

37.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.075 ± 0.053

1.044 ± 0.017

5.421 ± 0.03

6.593 ± 0.043

4.624 ± 0.035

7.78 ± 0.048

1.587 ± 0.016

7.234 ± 0.039

6.09 ± 0.037

9.644 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.383 ± 0.021

4.146 ± 0.037

4.359 ± 0.032

2.817 ± 0.023

5.003 ± 0.036

6.472 ± 0.041

5.032 ± 0.046

6.103 ± 0.034

1.147 ± 0.014

3.444 ± 0.031

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski