Amino acid dipepetide frequency for Mycobacterium phage Job42

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.856AlaAla: 13.856 ± 1.906
1.042AlaCys: 1.042 ± 0.224
5.99AlaAsp: 5.99 ± 0.606
7.657AlaGlu: 7.657 ± 0.708
2.917AlaPhe: 2.917 ± 0.346
9.376AlaGly: 9.376 ± 1.146
2.292AlaHis: 2.292 ± 0.376
4.323AlaIle: 4.323 ± 0.43
4.636AlaLys: 4.636 ± 0.477
8.074AlaLeu: 8.074 ± 0.744
2.604AlaMet: 2.604 ± 0.404
3.073AlaAsn: 3.073 ± 0.538
4.48AlaPro: 4.48 ± 0.572
3.698AlaGln: 3.698 ± 0.469
7.136AlaArg: 7.136 ± 0.77
5.886AlaSer: 5.886 ± 0.608
6.199AlaThr: 6.199 ± 0.53
7.032AlaVal: 7.032 ± 0.54
2.24AlaTrp: 2.24 ± 0.403
2.292AlaTyr: 2.292 ± 0.337
0.0AlaXaa: 0.0 ± 0.0
Cys
0.938CysAla: 0.938 ± 0.282
0.156CysCys: 0.156 ± 0.083
1.094CysAsp: 1.094 ± 0.234
1.094CysGlu: 1.094 ± 0.246
0.26CysPhe: 0.26 ± 0.123
1.719CysGly: 1.719 ± 0.371
0.26CysHis: 0.26 ± 0.1
0.26CysIle: 0.26 ± 0.111
0.677CysLys: 0.677 ± 0.181
0.886CysLeu: 0.886 ± 0.253
0.156CysMet: 0.156 ± 0.097
0.573CysAsn: 0.573 ± 0.186
1.198CysPro: 1.198 ± 0.242
0.417CysGln: 0.417 ± 0.185
0.938CysArg: 0.938 ± 0.25
0.729CysSer: 0.729 ± 0.257
0.677CysThr: 0.677 ± 0.228
0.729CysVal: 0.729 ± 0.182
0.208CysTrp: 0.208 ± 0.1
0.26CysTyr: 0.26 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
6.928AspAla: 6.928 ± 0.576
0.99AspCys: 0.99 ± 0.268
4.428AspAsp: 4.428 ± 0.497
3.855AspGlu: 3.855 ± 0.57
1.094AspPhe: 1.094 ± 0.185
6.251AspGly: 6.251 ± 0.518
1.354AspHis: 1.354 ± 0.266
2.448AspIle: 2.448 ± 0.346
1.823AspLys: 1.823 ± 0.307
5.834AspLeu: 5.834 ± 0.672
1.146AspMet: 1.146 ± 0.26
1.719AspAsn: 1.719 ± 0.338
4.636AspPro: 4.636 ± 0.5
2.396AspGln: 2.396 ± 0.301
5.053AspArg: 5.053 ± 0.612
2.865AspSer: 2.865 ± 0.441
3.386AspThr: 3.386 ± 0.368
4.48AspVal: 4.48 ± 0.599
1.719AspTrp: 1.719 ± 0.255
1.771AspTyr: 1.771 ± 0.291
0.0AspXaa: 0.0 ± 0.0
Glu
6.459GluAla: 6.459 ± 0.637
0.99GluCys: 0.99 ± 0.289
3.125GluAsp: 3.125 ± 0.292
2.657GluGlu: 2.657 ± 0.432
2.24GluPhe: 2.24 ± 0.323
3.49GluGly: 3.49 ± 0.419
1.198GluHis: 1.198 ± 0.304
2.761GluIle: 2.761 ± 0.316
1.771GluLys: 1.771 ± 0.303
6.303GluLeu: 6.303 ± 0.634
1.563GluMet: 1.563 ± 0.245
1.771GluAsn: 1.771 ± 0.286
3.073GluPro: 3.073 ± 0.446
2.865GluGln: 2.865 ± 0.344
5.417GluArg: 5.417 ± 0.534
3.49GluSer: 3.49 ± 0.409
3.855GluThr: 3.855 ± 0.454
4.271GluVal: 4.271 ± 0.514
1.146GluTrp: 1.146 ± 0.225
1.719GluTyr: 1.719 ± 0.342
0.0GluXaa: 0.0 ± 0.0
Phe
2.865PheAla: 2.865 ± 0.399
0.26PheCys: 0.26 ± 0.132
2.5PheAsp: 2.5 ± 0.436
1.458PheGlu: 1.458 ± 0.284
0.886PhePhe: 0.886 ± 0.259
3.334PheGly: 3.334 ± 0.577
0.625PheHis: 0.625 ± 0.222
1.042PheIle: 1.042 ± 0.304
1.198PheLys: 1.198 ± 0.285
1.927PheLeu: 1.927 ± 0.303
0.573PheMet: 0.573 ± 0.172
1.302PheAsn: 1.302 ± 0.333
1.511PhePro: 1.511 ± 0.332
1.042PheGln: 1.042 ± 0.323
1.511PheArg: 1.511 ± 0.238
1.354PheSer: 1.354 ± 0.245
2.031PheThr: 2.031 ± 0.303
1.875PheVal: 1.875 ± 0.249
0.677PheTrp: 0.677 ± 0.191
0.833PheTyr: 0.833 ± 0.266
0.0PheXaa: 0.0 ± 0.0
Gly
9.689GlyAla: 9.689 ± 1.079
1.25GlyCys: 1.25 ± 0.283
5.834GlyAsp: 5.834 ± 0.508
4.584GlyGlu: 4.584 ± 0.569
2.657GlyPhe: 2.657 ± 0.47
9.689GlyGly: 9.689 ± 1.626
1.875GlyHis: 1.875 ± 0.277
3.594GlyIle: 3.594 ± 0.439
2.552GlyLys: 2.552 ± 0.323
5.886GlyLeu: 5.886 ± 0.533
2.292GlyMet: 2.292 ± 0.496
3.073GlyAsn: 3.073 ± 0.386
3.907GlyPro: 3.907 ± 0.531
2.292GlyGln: 2.292 ± 0.46
5.626GlyArg: 5.626 ± 0.647
5.105GlySer: 5.105 ± 0.695
5.938GlyThr: 5.938 ± 0.855
6.615GlyVal: 6.615 ± 0.621
2.552GlyTrp: 2.552 ± 0.377
2.188GlyTyr: 2.188 ± 0.36
0.0GlyXaa: 0.0 ± 0.0
His
1.563HisAla: 1.563 ± 0.315
0.365HisCys: 0.365 ± 0.158
1.406HisAsp: 1.406 ± 0.256
1.25HisGlu: 1.25 ± 0.27
0.521HisPhe: 0.521 ± 0.134
1.823HisGly: 1.823 ± 0.265
0.729HisHis: 0.729 ± 0.257
1.406HisIle: 1.406 ± 0.292
1.25HisLys: 1.25 ± 0.274
1.719HisLeu: 1.719 ± 0.265
0.521HisMet: 0.521 ± 0.146
0.99HisAsn: 0.99 ± 0.252
1.302HisPro: 1.302 ± 0.247
0.625HisGln: 0.625 ± 0.183
1.511HisArg: 1.511 ± 0.33
0.833HisSer: 0.833 ± 0.199
1.406HisThr: 1.406 ± 0.254
1.563HisVal: 1.563 ± 0.285
0.469HisTrp: 0.469 ± 0.16
0.886HisTyr: 0.886 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
5.469IleAla: 5.469 ± 0.584
0.625IleCys: 0.625 ± 0.232
3.646IleAsp: 3.646 ± 0.511
3.125IleGlu: 3.125 ± 0.359
0.833IlePhe: 0.833 ± 0.253
4.063IleGly: 4.063 ± 0.398
1.406IleHis: 1.406 ± 0.306
1.406IleIle: 1.406 ± 0.289
1.302IleLys: 1.302 ± 0.259
2.709IleLeu: 2.709 ± 0.385
0.26IleMet: 0.26 ± 0.108
1.823IleAsn: 1.823 ± 0.322
2.709IlePro: 2.709 ± 0.357
1.563IleGln: 1.563 ± 0.266
2.344IleArg: 2.344 ± 0.323
2.031IleSer: 2.031 ± 0.289
3.334IleThr: 3.334 ± 0.39
3.386IleVal: 3.386 ± 0.325
0.781IleTrp: 0.781 ± 0.202
0.729IleTyr: 0.729 ± 0.194
0.0IleXaa: 0.0 ± 0.0
Lys
4.011LysAla: 4.011 ± 0.478
0.417LysCys: 0.417 ± 0.149
2.084LysAsp: 2.084 ± 0.312
1.719LysGlu: 1.719 ± 0.259
1.25LysPhe: 1.25 ± 0.208
2.396LysGly: 2.396 ± 0.29
1.042LysHis: 1.042 ± 0.237
0.886LysIle: 0.886 ± 0.26
1.25LysLys: 1.25 ± 0.285
2.917LysLeu: 2.917 ± 0.505
0.729LysMet: 0.729 ± 0.172
1.25LysAsn: 1.25 ± 0.284
2.5LysPro: 2.5 ± 0.336
1.563LysGln: 1.563 ± 0.242
2.5LysArg: 2.5 ± 0.368
2.292LysSer: 2.292 ± 0.33
2.657LysThr: 2.657 ± 0.384
2.552LysVal: 2.552 ± 0.38
1.094LysTrp: 1.094 ± 0.25
0.677LysTyr: 0.677 ± 0.192
0.0LysXaa: 0.0 ± 0.0
Leu
8.386LeuAla: 8.386 ± 0.935
0.781LeuCys: 0.781 ± 0.271
5.001LeuAsp: 5.001 ± 0.59
4.48LeuGlu: 4.48 ± 0.511
2.188LeuPhe: 2.188 ± 0.308
5.886LeuGly: 5.886 ± 0.609
0.886LeuHis: 0.886 ± 0.195
3.073LeuIle: 3.073 ± 0.372
2.396LeuLys: 2.396 ± 0.401
5.001LeuLeu: 5.001 ± 0.616
1.511LeuMet: 1.511 ± 0.289
2.709LeuAsn: 2.709 ± 0.398
5.313LeuPro: 5.313 ± 0.652
2.813LeuGln: 2.813 ± 0.5
5.417LeuArg: 5.417 ± 0.642
5.053LeuSer: 5.053 ± 0.494
5.521LeuThr: 5.521 ± 0.548
5.782LeuVal: 5.782 ± 0.548
1.198LeuTrp: 1.198 ± 0.263
2.188LeuTyr: 2.188 ± 0.373
0.0LeuXaa: 0.0 ± 0.0
Met
1.667MetAla: 1.667 ± 0.33
0.26MetCys: 0.26 ± 0.146
1.146MetAsp: 1.146 ± 0.253
0.886MetGlu: 0.886 ± 0.217
0.469MetPhe: 0.469 ± 0.163
1.771MetGly: 1.771 ± 0.309
0.26MetHis: 0.26 ± 0.109
1.042MetIle: 1.042 ± 0.236
0.938MetLys: 0.938 ± 0.225
1.406MetLeu: 1.406 ± 0.256
0.573MetMet: 0.573 ± 0.237
0.833MetAsn: 0.833 ± 0.204
1.354MetPro: 1.354 ± 0.256
0.521MetGln: 0.521 ± 0.174
1.563MetArg: 1.563 ± 0.269
2.761MetSer: 2.761 ± 0.409
2.188MetThr: 2.188 ± 0.328
1.563MetVal: 1.563 ± 0.322
0.417MetTrp: 0.417 ± 0.156
0.313MetTyr: 0.313 ± 0.122
0.0MetXaa: 0.0 ± 0.0
Asn
3.23AsnAla: 3.23 ± 0.398
0.417AsnCys: 0.417 ± 0.131
1.771AsnAsp: 1.771 ± 0.298
2.031AsnGlu: 2.031 ± 0.337
0.677AsnPhe: 0.677 ± 0.254
3.646AsnGly: 3.646 ± 0.615
0.886AsnHis: 0.886 ± 0.168
1.563AsnIle: 1.563 ± 0.453
0.99AsnLys: 0.99 ± 0.245
2.604AsnLeu: 2.604 ± 0.385
0.781AsnMet: 0.781 ± 0.202
1.719AsnAsn: 1.719 ± 0.351
2.604AsnPro: 2.604 ± 0.325
1.042AsnGln: 1.042 ± 0.342
2.709AsnArg: 2.709 ± 0.393
1.146AsnSer: 1.146 ± 0.223
2.188AsnThr: 2.188 ± 0.31
1.615AsnVal: 1.615 ± 0.282
0.469AsnTrp: 0.469 ± 0.145
0.573AsnTyr: 0.573 ± 0.129
0.0AsnXaa: 0.0 ± 0.0
Pro
5.782ProAla: 5.782 ± 0.496
0.729ProCys: 0.729 ± 0.214
4.167ProAsp: 4.167 ± 0.488
4.271ProGlu: 4.271 ± 0.467
1.667ProPhe: 1.667 ± 0.286
6.407ProGly: 6.407 ± 0.665
1.563ProHis: 1.563 ± 0.266
2.24ProIle: 2.24 ± 0.288
2.031ProLys: 2.031 ± 0.332
4.271ProLeu: 4.271 ± 0.561
1.354ProMet: 1.354 ± 0.329
1.667ProAsn: 1.667 ± 0.265
4.063ProPro: 4.063 ± 0.634
2.604ProGln: 2.604 ± 0.319
2.813ProArg: 2.813 ± 0.457
3.23ProSer: 3.23 ± 0.482
3.594ProThr: 3.594 ± 0.424
4.688ProVal: 4.688 ± 0.488
1.198ProTrp: 1.198 ± 0.25
1.823ProTyr: 1.823 ± 0.259
0.0ProXaa: 0.0 ± 0.0
Gln
4.167GlnAla: 4.167 ± 0.584
0.469GlnCys: 0.469 ± 0.18
1.302GlnAsp: 1.302 ± 0.248
1.667GlnGlu: 1.667 ± 0.254
0.833GlnPhe: 0.833 ± 0.21
2.604GlnGly: 2.604 ± 0.405
0.938GlnHis: 0.938 ± 0.181
1.979GlnIle: 1.979 ± 0.347
1.354GlnLys: 1.354 ± 0.24
3.125GlnLeu: 3.125 ± 0.513
0.886GlnMet: 0.886 ± 0.209
0.886GlnAsn: 0.886 ± 0.177
2.552GlnPro: 2.552 ± 0.407
2.084GlnGln: 2.084 ± 0.471
2.969GlnArg: 2.969 ± 0.463
2.552GlnSer: 2.552 ± 0.342
1.667GlnThr: 1.667 ± 0.275
2.5GlnVal: 2.5 ± 0.355
0.677GlnTrp: 0.677 ± 0.168
0.938GlnTyr: 0.938 ± 0.24
0.0GlnXaa: 0.0 ± 0.0
Arg
6.928ArgAla: 6.928 ± 0.705
1.146ArgCys: 1.146 ± 0.325
4.74ArgAsp: 4.74 ± 0.665
5.157ArgGlu: 5.157 ± 0.64
2.657ArgPhe: 2.657 ± 0.404
4.063ArgGly: 4.063 ± 0.405
1.354ArgHis: 1.354 ± 0.435
3.907ArgIle: 3.907 ± 0.522
2.5ArgLys: 2.5 ± 0.297
5.001ArgLeu: 5.001 ± 0.486
2.24ArgMet: 2.24 ± 0.387
1.719ArgAsn: 1.719 ± 0.393
3.594ArgPro: 3.594 ± 0.411
2.604ArgGln: 2.604 ± 0.451
5.313ArgArg: 5.313 ± 0.651
4.375ArgSer: 4.375 ± 0.496
2.969ArgThr: 2.969 ± 0.433
4.948ArgVal: 4.948 ± 0.497
1.875ArgTrp: 1.875 ± 0.341
2.292ArgTyr: 2.292 ± 0.318
0.0ArgXaa: 0.0 ± 0.0
Ser
5.261SerAla: 5.261 ± 0.653
0.677SerCys: 0.677 ± 0.25
3.959SerAsp: 3.959 ± 0.502
3.802SerGlu: 3.802 ± 0.5
2.552SerPhe: 2.552 ± 0.472
6.094SerGly: 6.094 ± 0.924
1.146SerHis: 1.146 ± 0.218
3.021SerIle: 3.021 ± 0.464
2.396SerLys: 2.396 ± 0.424
3.907SerLeu: 3.907 ± 0.475
1.354SerMet: 1.354 ± 0.231
1.823SerAsn: 1.823 ± 0.343
3.855SerPro: 3.855 ± 0.433
1.771SerGln: 1.771 ± 0.314
3.177SerArg: 3.177 ± 0.417
4.428SerSer: 4.428 ± 0.541
3.177SerThr: 3.177 ± 0.409
4.375SerVal: 4.375 ± 0.5
1.406SerTrp: 1.406 ± 0.262
1.302SerTyr: 1.302 ± 0.256
0.0SerXaa: 0.0 ± 0.0
Thr
5.886ThrAla: 5.886 ± 0.706
0.729ThrCys: 0.729 ± 0.233
3.23ThrAsp: 3.23 ± 0.494
3.49ThrGlu: 3.49 ± 0.386
1.615ThrPhe: 1.615 ± 0.317
6.042ThrGly: 6.042 ± 0.548
1.719ThrHis: 1.719 ± 0.274
2.969ThrIle: 2.969 ± 0.512
2.396ThrLys: 2.396 ± 0.371
4.48ThrLeu: 4.48 ± 0.484
0.833ThrMet: 0.833 ± 0.211
1.875ThrAsn: 1.875 ± 0.376
4.323ThrPro: 4.323 ± 0.541
1.667ThrGln: 1.667 ± 0.293
4.48ThrArg: 4.48 ± 0.459
3.698ThrSer: 3.698 ± 0.42
4.532ThrThr: 4.532 ± 0.638
5.678ThrVal: 5.678 ± 0.648
1.354ThrTrp: 1.354 ± 0.259
2.084ThrTyr: 2.084 ± 0.364
0.0ThrXaa: 0.0 ± 0.0
Val
7.084ValAla: 7.084 ± 0.549
1.198ValCys: 1.198 ± 0.268
5.521ValAsp: 5.521 ± 0.578
4.271ValGlu: 4.271 ± 0.569
1.979ValPhe: 1.979 ± 0.385
6.042ValGly: 6.042 ± 0.632
1.719ValHis: 1.719 ± 0.293
3.542ValIle: 3.542 ± 0.415
2.604ValLys: 2.604 ± 0.384
5.469ValLeu: 5.469 ± 0.709
1.302ValMet: 1.302 ± 0.242
2.552ValAsn: 2.552 ± 0.331
4.271ValPro: 4.271 ± 0.434
2.657ValGln: 2.657 ± 0.317
5.209ValArg: 5.209 ± 0.605
4.844ValSer: 4.844 ± 0.625
4.74ValThr: 4.74 ± 0.454
6.042ValVal: 6.042 ± 0.696
1.511ValTrp: 1.511 ± 0.347
1.406ValTyr: 1.406 ± 0.319
0.0ValXaa: 0.0 ± 0.0
Trp
2.136TrpAla: 2.136 ± 0.308
0.365TrpCys: 0.365 ± 0.143
1.25TrpAsp: 1.25 ± 0.311
0.938TrpGlu: 0.938 ± 0.226
0.781TrpPhe: 0.781 ± 0.171
0.729TrpGly: 0.729 ± 0.21
0.521TrpHis: 0.521 ± 0.17
1.094TrpIle: 1.094 ± 0.215
0.833TrpLys: 0.833 ± 0.157
1.771TrpLeu: 1.771 ± 0.302
0.938TrpMet: 0.938 ± 0.206
0.573TrpAsn: 0.573 ± 0.208
1.146TrpPro: 1.146 ± 0.239
1.094TrpGln: 1.094 ± 0.263
1.927TrpArg: 1.927 ± 0.358
1.511TrpSer: 1.511 ± 0.301
1.354TrpThr: 1.354 ± 0.25
1.979TrpVal: 1.979 ± 0.415
0.833TrpTrp: 0.833 ± 0.205
0.521TrpTyr: 0.521 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.5TyrAla: 2.5 ± 0.356
0.365TyrCys: 0.365 ± 0.139
1.979TyrAsp: 1.979 ± 0.352
1.771TyrGlu: 1.771 ± 0.319
0.729TyrPhe: 0.729 ± 0.194
1.771TyrGly: 1.771 ± 0.378
0.365TyrHis: 0.365 ± 0.12
0.99TyrIle: 0.99 ± 0.214
0.886TyrLys: 0.886 ± 0.246
2.292TyrLeu: 2.292 ± 0.392
0.26TyrMet: 0.26 ± 0.12
0.729TyrAsn: 0.729 ± 0.223
1.771TyrPro: 1.771 ± 0.312
0.833TyrGln: 0.833 ± 0.222
1.771TyrArg: 1.771 ± 0.308
1.354TyrSer: 1.354 ± 0.292
1.667TyrThr: 1.667 ± 0.336
2.292TyrVal: 2.292 ± 0.283
0.469TyrTrp: 0.469 ± 0.158
0.677TyrTyr: 0.677 ± 0.173
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 106 proteins (19199 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski