Corynebacterium vitaeruminis DSM 20294
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2565 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5Y3R4|W5Y3R4_9CORY Ribonucleoside-diphosphate reductase subunit beta OS=Corynebacterium vitaeruminis DSM 20294 OX=1224164 GN=B843_10600 PE=3 SV=1
MM1 pKa = 7.43 LSVMVTGLTVFSPASASAQDD21 pKa = 3.92 SFTDD25 pKa = 4.01 PSLGDD30 pKa = 3.86 ALTDD34 pKa = 4.05 LATALDD40 pKa = 4.22 DD41 pKa = 4.33 QNAADD46 pKa = 4.0 TTDD49 pKa = 3.13 ATGLDD54 pKa = 3.64 VSASVDD60 pKa = 3.24 AADD63 pKa = 3.71 ITDD66 pKa = 3.57 SAEE69 pKa = 4.09 AVSVDD74 pKa = 3.63 PGAAALIDD82 pKa = 3.56 QSLQGVGQSIGEE94 pKa = 4.0 LDD96 pKa = 3.8 AATGLAFGPQLAFVAPMAVTDD117 pKa = 4.64 PFAPTPVFTEE127 pKa = 4.08 DD128 pKa = 4.83 FEE130 pKa = 6.21 NGTNNTTPTKK140 pKa = 10.44 LSDD143 pKa = 3.89 YY144 pKa = 10.73 IGATNSTTYY153 pKa = 10.39 SASGYY158 pKa = 8.22 WLNPGYY164 pKa = 10.83 CNGFITSFATNLSQDD179 pKa = 4.41 NIDD182 pKa = 3.8 KK183 pKa = 10.61 LYY185 pKa = 10.42 CGRR188 pKa = 11.84 NGGFDD193 pKa = 3.69 PGDD196 pKa = 3.38 YY197 pKa = 9.34 WAVRR201 pKa = 11.84 SKK203 pKa = 11.21 VLALGSFSNVPAGTIVTGNEE223 pKa = 3.77 SATGKK228 pKa = 10.21 FNHH231 pKa = 6.82 ALSTNTSGGTPSGVMFQTTKK251 pKa = 10.93 DD252 pKa = 3.0 IVLPGSSGGRR262 pKa = 11.84 FYY264 pKa = 11.5 AFSVDD269 pKa = 3.89 AAVTGCSNTGSGNSGQVNPNMYY291 pKa = 9.76 FQMVQGGKK299 pKa = 8.57 TISLPNNGNIDD310 pKa = 3.74 PCSSPDD316 pKa = 3.2 SRR318 pKa = 11.84 QFDD321 pKa = 3.44 VTAQYY326 pKa = 10.96 GYY328 pKa = 10.74 QGGSWGTQSGWIATGKK344 pKa = 10.63 VGTFYY349 pKa = 11.03 SGAFALDD356 pKa = 3.47 NSASVGLILQNSSYY370 pKa = 11.24 ASSTGRR376 pKa = 11.84 GIANGSSGTYY386 pKa = 9.44 PNSPTGRR393 pKa = 11.84 RR394 pKa = 11.84 TT395 pKa = 3.12
Molecular weight: 40.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.834
IPC_protein 3.872
Toseland 3.617
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.872
Rodwell 3.694
Grimsley 3.528
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.329
Thurlkill 3.694
EMBOSS 3.872
Sillero 3.999
Patrickios 0.896
IPC_peptide 3.872
IPC2_peptide 3.961
IPC2.peptide.svr19 3.869
Protein with the highest isoelectric point:
>tr|W5Y5R0|W5Y5R0_9CORY 50S ribosomal protein L2 OS=Corynebacterium vitaeruminis DSM 20294 OX=1224164 GN=rplB PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.72 HH17 pKa = 5.63 RR18 pKa = 11.84 KK19 pKa = 7.56 LLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2565
0
2565
836113
28
3016
326.0
35.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.086 ± 0.071
0.761 ± 0.014
5.831 ± 0.04
6.28 ± 0.054
3.409 ± 0.031
8.544 ± 0.041
2.062 ± 0.02
5.005 ± 0.034
3.364 ± 0.035
9.747 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.248 ± 0.022
2.712 ± 0.029
4.944 ± 0.035
3.171 ± 0.026
6.103 ± 0.045
5.877 ± 0.039
5.951 ± 0.041
8.276 ± 0.045
1.377 ± 0.021
2.254 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here