Amino acid dipepetide frequency for Clostridiales bacterium PH28_bin88

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.295AlaAla: 10.295 ± 0.135	1.133AlaCys: 1.133 ± 0.033	4.134AlaAsp: 4.134 ± 0.061	6.213AlaGlu: 6.213 ± 0.081	3.224AlaPhe: 3.224 ± 0.052	9.581AlaGly: 9.581 ± 0.103	1.569AlaHis: 1.569 ± 0.037	5.24AlaIle: 5.24 ± 0.071	3.978AlaLys: 3.978 ± 0.07	9.914AlaLeu: 9.914 ± 0.107	2.648AlaMet: 2.648 ± 0.045	2.425AlaAsn: 2.425 ± 0.047	3.145AlaPro: 3.145 ± 0.054	2.905AlaGln: 2.905 ± 0.049	6.44AlaArg: 6.44 ± 0.084	4.326AlaSer: 4.326 ± 0.063	4.244AlaThr: 4.244 ± 0.061	8.624AlaVal: 8.624 ± 0.086	1.01AlaTrp: 1.01 ± 0.031	2.471AlaTyr: 2.471 ± 0.038	0.003AlaXaa: 0.003 ± 0.002
Cys	0.823CysAla: 0.823 ± 0.026	0.189CysCys: 0.189 ± 0.013	0.474CysAsp: 0.474 ± 0.018	0.481CysGlu: 0.481 ± 0.021	0.369CysPhe: 0.369 ± 0.018	1.242CysGly: 1.242 ± 0.037	0.4CysHis: 0.4 ± 0.03	0.554CysIle: 0.554 ± 0.02	0.341CysLys: 0.341 ± 0.018	1.045CysLeu: 1.045 ± 0.032	0.244CysMet: 0.244 ± 0.013	0.326CysAsn: 0.326 ± 0.015	0.791CysPro: 0.791 ± 0.033	0.418CysGln: 0.418 ± 0.019	0.856CysArg: 0.856 ± 0.027	0.575CysSer: 0.575 ± 0.022	0.574CysThr: 0.574 ± 0.024	0.688CysVal: 0.688 ± 0.031	0.125CysTrp: 0.125 ± 0.009	0.338CysTyr: 0.338 ± 0.018	0.0CysXaa: 0.0 ± 0.0
Asp	3.83AspAla: 3.83 ± 0.058	0.493AspCys: 0.493 ± 0.023	1.978AspAsp: 1.978 ± 0.048	3.078AspGlu: 3.078 ± 0.056	1.997AspPhe: 1.997 ± 0.039	3.685AspGly: 3.685 ± 0.055	0.985AspHis: 0.985 ± 0.029	3.105AspIle: 3.105 ± 0.056	1.965AspLys: 1.965 ± 0.044	5.62AspLeu: 5.62 ± 0.082	1.259AspMet: 1.259 ± 0.027	1.297AspAsn: 1.297 ± 0.036	2.736AspPro: 2.736 ± 0.051	1.61AspGln: 1.61 ± 0.035	3.197AspArg: 3.197 ± 0.052	1.905AspSer: 1.905 ± 0.034	2.283AspThr: 2.283 ± 0.04	3.661AspVal: 3.661 ± 0.053	0.614AspTrp: 0.614 ± 0.022	1.65AspTyr: 1.65 ± 0.045	0.001AspXaa: 0.001 ± 0.001
Glu	6.843GluAla: 6.843 ± 0.084	0.533GluCys: 0.533 ± 0.023	3.115GluAsp: 3.115 ± 0.06	5.781GluGlu: 5.781 ± 0.077	2.14GluPhe: 2.14 ± 0.044	5.025GluGly: 5.025 ± 0.072	1.261GluHis: 1.261 ± 0.031	4.442GluIle: 4.442 ± 0.067	4.223GluLys: 4.223 ± 0.063	6.803GluLeu: 6.803 ± 0.08	2.126GluMet: 2.126 ± 0.04	2.225GluAsn: 2.225 ± 0.042	2.438GluPro: 2.438 ± 0.039	2.76GluGln: 2.76 ± 0.055	4.65GluArg: 4.65 ± 0.065	2.516GluSer: 2.516 ± 0.047	3.342GluThr: 3.342 ± 0.052	6.238GluVal: 6.238 ± 0.07	0.684GluTrp: 0.684 ± 0.023	1.887GluTyr: 1.887 ± 0.039	0.002GluXaa: 0.002 ± 0.001
Phe	3.124PheAla: 3.124 ± 0.049	0.474PheCys: 0.474 ± 0.018	1.817PheAsp: 1.817 ± 0.04	1.83PheGlu: 1.83 ± 0.045	1.603PhePhe: 1.603 ± 0.043	3.097PheGly: 3.097 ± 0.047	0.778PheHis: 0.778 ± 0.025	2.106PheIle: 2.106 ± 0.042	1.477PheLys: 1.477 ± 0.033	3.943PheLeu: 3.943 ± 0.058	0.866PheMet: 0.866 ± 0.027	1.216PheAsn: 1.216 ± 0.03	1.823PhePro: 1.823 ± 0.042	1.174PheGln: 1.174 ± 0.033	2.152PheArg: 2.152 ± 0.042	2.29PheSer: 2.29 ± 0.052	2.137PheThr: 2.137 ± 0.043	2.495PheVal: 2.495 ± 0.053	0.476PheTrp: 0.476 ± 0.021	1.212PheTyr: 1.212 ± 0.035	0.001PheXaa: 0.001 ± 0.001
Gly	7.177GlyAla: 7.177 ± 0.087	1.172GlyCys: 1.172 ± 0.034	3.778GlyAsp: 3.778 ± 0.056	5.558GlyGlu: 5.558 ± 0.073	3.262GlyPhe: 3.262 ± 0.054	6.965GlyGly: 6.965 ± 0.103	1.589GlyHis: 1.589 ± 0.036	5.803GlyIle: 5.803 ± 0.077	4.795GlyLys: 4.795 ± 0.067	9.16GlyLeu: 9.16 ± 0.099	2.692GlyMet: 2.692 ± 0.048	2.595GlyAsn: 2.595 ± 0.048	2.833GlyPro: 2.833 ± 0.057	2.995GlyGln: 2.995 ± 0.053	5.305GlyArg: 5.305 ± 0.075	4.341GlySer: 4.341 ± 0.053	4.591GlyThr: 4.591 ± 0.063	6.891GlyVal: 6.891 ± 0.081	1.024GlyTrp: 1.024 ± 0.03	2.977GlyTyr: 2.977 ± 0.053	0.003GlyXaa: 0.003 ± 0.001
His	1.427HisAla: 1.427 ± 0.036	0.29HisCys: 0.29 ± 0.016	0.828HisAsp: 0.828 ± 0.026	1.084HisGlu: 1.084 ± 0.028	0.743HisPhe: 0.743 ± 0.03	1.63HisGly: 1.63 ± 0.038	0.557HisHis: 0.557 ± 0.022	1.013HisIle: 1.013 ± 0.028	0.624HisLys: 0.624 ± 0.023	2.27HisLeu: 2.27 ± 0.042	0.459HisMet: 0.459 ± 0.02	0.581HisAsn: 0.581 ± 0.021	1.345HisPro: 1.345 ± 0.035	0.86HisGln: 0.86 ± 0.026	1.404HisArg: 1.404 ± 0.035	0.894HisSer: 0.894 ± 0.026	0.982HisThr: 0.982 ± 0.025	1.315HisVal: 1.315 ± 0.029	0.252HisTrp: 0.252 ± 0.015	0.631HisTyr: 0.631 ± 0.021	0.002HisXaa: 0.002 ± 0.001
Ile	5.29IleAla: 5.29 ± 0.071	0.594IleCys: 0.594 ± 0.022	2.782IleAsp: 2.782 ± 0.046	3.304IleGlu: 3.304 ± 0.059	2.033IlePhe: 2.033 ± 0.043	4.594IleGly: 4.594 ± 0.077	1.123IleHis: 1.123 ± 0.031	3.534IleIle: 3.534 ± 0.064	2.928IleLys: 2.928 ± 0.052	5.843IleLeu: 5.843 ± 0.084	1.417IleMet: 1.417 ± 0.037	2.197IleAsn: 2.197 ± 0.053	3.331IlePro: 3.331 ± 0.05	1.851IleGln: 1.851 ± 0.046	3.543IleArg: 3.543 ± 0.05	3.428IleSer: 3.428 ± 0.053	3.594IleThr: 3.594 ± 0.055	4.08IleVal: 4.08 ± 0.058	0.499IleTrp: 0.499 ± 0.018	1.624IleTyr: 1.624 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	4.249LysAla: 4.249 ± 0.068	0.414LysCys: 0.414 ± 0.016	2.375LysAsp: 2.375 ± 0.049	4.003LysGlu: 4.003 ± 0.071	1.315LysPhe: 1.315 ± 0.038	3.682LysGly: 3.682 ± 0.056	0.869LysHis: 0.869 ± 0.025	2.736LysIle: 2.736 ± 0.045	2.784LysLys: 2.784 ± 0.058	4.208LysLeu: 4.208 ± 0.058	1.242LysMet: 1.242 ± 0.037	1.617LysAsn: 1.617 ± 0.034	2.134LysPro: 2.134 ± 0.041	1.63LysGln: 1.63 ± 0.038	2.558LysArg: 2.558 ± 0.049	2.243LysSer: 2.243 ± 0.045	2.509LysThr: 2.509 ± 0.045	4.144LysVal: 4.144 ± 0.057	0.477LysTrp: 0.477 ± 0.021	1.449LysTyr: 1.449 ± 0.034	0.002LysXaa: 0.002 ± 0.001
Leu	11.882LeuAla: 11.882 ± 0.131	0.986LeuCys: 0.986 ± 0.031	5.066LeuAsp: 5.066 ± 0.078	7.403LeuGlu: 7.403 ± 0.086	3.557LeuPhe: 3.557 ± 0.06	8.718LeuGly: 8.718 ± 0.091	1.785LeuHis: 1.785 ± 0.043	5.228LeuIle: 5.228 ± 0.069	5.036LeuLys: 5.036 ± 0.063	10.7LeuLeu: 10.7 ± 0.132	2.582LeuMet: 2.582 ± 0.048	3.107LeuAsn: 3.107 ± 0.053	5.263LeuPro: 5.263 ± 0.071	3.562LeuGln: 3.562 ± 0.058	6.326LeuArg: 6.326 ± 0.068	5.63LeuSer: 5.63 ± 0.074	5.388LeuThr: 5.388 ± 0.061	9.271LeuVal: 9.271 ± 0.106	1.043LeuTrp: 1.043 ± 0.032	2.602LeuTyr: 2.602 ± 0.047	0.004LeuXaa: 0.004 ± 0.002
Met	2.897MetAla: 2.897 ± 0.048	0.215MetCys: 0.215 ± 0.015	1.378MetAsp: 1.378 ± 0.036	2.014MetGlu: 2.014 ± 0.044	0.77MetPhe: 0.77 ± 0.025	2.387MetGly: 2.387 ± 0.048	0.471MetHis: 0.471 ± 0.02	1.38MetIle: 1.38 ± 0.035	1.306MetLys: 1.306 ± 0.031	2.594MetLeu: 2.594 ± 0.051	0.687MetMet: 0.687 ± 0.025	0.797MetAsn: 0.797 ± 0.024	1.186MetPro: 1.186 ± 0.032	0.893MetGln: 0.893 ± 0.026	1.507MetArg: 1.507 ± 0.032	1.346MetSer: 1.346 ± 0.032	1.354MetThr: 1.354 ± 0.035	2.586MetVal: 2.586 ± 0.049	0.209MetTrp: 0.209 ± 0.013	0.557MetTyr: 0.557 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.361AsnAla: 2.361 ± 0.048	0.41AsnCys: 0.41 ± 0.019	1.234AsnAsp: 1.234 ± 0.036	1.58AsnGlu: 1.58 ± 0.034	1.129AsnPhe: 1.129 ± 0.034	2.375AsnGly: 2.375 ± 0.047	0.596AsnHis: 0.596 ± 0.024	1.968AsnIle: 1.968 ± 0.04	1.37AsnLys: 1.37 ± 0.032	3.569AsnLeu: 3.569 ± 0.052	0.756AsnMet: 0.756 ± 0.026	0.962AsnAsn: 0.962 ± 0.031	2.094AsnPro: 2.094 ± 0.037	1.075AsnGln: 1.075 ± 0.028	2.072AsnArg: 2.072 ± 0.041	1.522AsnSer: 1.522 ± 0.039	1.564AsnThr: 1.564 ± 0.03	2.215AsnVal: 2.215 ± 0.038	0.392AsnTrp: 0.392 ± 0.017	1.029AsnTyr: 1.029 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	4.491ProAla: 4.491 ± 0.065	0.43ProCys: 0.43 ± 0.02	2.636ProAsp: 2.636 ± 0.052	4.103ProGlu: 4.103 ± 0.071	1.775ProPhe: 1.775 ± 0.035	4.891ProGly: 4.891 ± 0.071	0.91ProHis: 0.91 ± 0.029	2.041ProIle: 2.041 ± 0.046	1.72ProLys: 1.72 ± 0.039	4.433ProLeu: 4.433 ± 0.065	1.008ProMet: 1.008 ± 0.026	1.227ProAsn: 1.227 ± 0.034	2.177ProPro: 2.177 ± 0.047	1.5ProGln: 1.5 ± 0.034	2.609ProArg: 2.609 ± 0.048	2.164ProSer: 2.164 ± 0.041	2.03ProThr: 2.03 ± 0.047	4.742ProVal: 4.742 ± 0.073	0.617ProTrp: 0.617 ± 0.025	1.416ProTyr: 1.416 ± 0.03	0.001ProXaa: 0.001 ± 0.001
Gln	3.796GlnAla: 3.796 ± 0.058	0.311GlnCys: 0.311 ± 0.016	1.634GlnAsp: 1.634 ± 0.036	2.993GlnGlu: 2.993 ± 0.049	0.977GlnPhe: 0.977 ± 0.03	2.815GlnGly: 2.815 ± 0.046	0.63GlnHis: 0.63 ± 0.023	1.814GlnIle: 1.814 ± 0.037	1.778GlnLys: 1.778 ± 0.037	3.299GlnLeu: 3.299 ± 0.054	0.943GlnMet: 0.943 ± 0.029	0.963GlnAsn: 0.963 ± 0.031	1.566GlnPro: 1.566 ± 0.036	1.597GlnGln: 1.597 ± 0.044	2.234GlnArg: 2.234 ± 0.045	1.423GlnSer: 1.423 ± 0.036	1.419GlnThr: 1.419 ± 0.035	3.746GlnVal: 3.746 ± 0.056	0.398GlnTrp: 0.398 ± 0.017	0.856GlnTyr: 0.856 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	4.909ArgAla: 4.909 ± 0.068	0.751ArgCys: 0.751 ± 0.026	3.008ArgAsp: 3.008 ± 0.055	5.689ArgGlu: 5.689 ± 0.077	2.353ArgPhe: 2.353 ± 0.047	4.585ArgGly: 4.585 ± 0.055	1.3ArgHis: 1.3 ± 0.03	3.591ArgIle: 3.591 ± 0.058	2.91ArgLys: 2.91 ± 0.04	7.052ArgLeu: 7.052 ± 0.086	1.735ArgMet: 1.735 ± 0.04	1.826ArgAsn: 1.826 ± 0.042	2.614ArgPro: 2.614 ± 0.056	2.903ArgGln: 2.903 ± 0.056	4.612ArgArg: 4.612 ± 0.074	2.813ArgSer: 2.813 ± 0.045	2.756ArgThr: 2.756 ± 0.049	5.721ArgVal: 5.721 ± 0.074	0.854ArgTrp: 0.854 ± 0.029	2.136ArgTyr: 2.136 ± 0.042	0.002ArgXaa: 0.002 ± 0.001
Ser	3.982SerAla: 3.982 ± 0.058	0.586SerCys: 0.586 ± 0.025	2.084SerAsp: 2.084 ± 0.04	2.707SerGlu: 2.707 ± 0.04	2.098SerPhe: 2.098 ± 0.041	4.7SerGly: 4.7 ± 0.067	1.045SerHis: 1.045 ± 0.026	2.836SerIle: 2.836 ± 0.052	2.026SerLys: 2.026 ± 0.041	5.681SerLeu: 5.681 ± 0.078	1.386SerMet: 1.386 ± 0.037	1.399SerAsn: 1.399 ± 0.037	2.65SerPro: 2.65 ± 0.042	1.708SerGln: 1.708 ± 0.03	3.479SerArg: 3.479 ± 0.062	2.656SerSer: 2.656 ± 0.046	2.528SerThr: 2.528 ± 0.045	3.646SerVal: 3.646 ± 0.056	0.599SerTrp: 0.599 ± 0.022	1.474SerTyr: 1.474 ± 0.035	0.001SerXaa: 0.001 ± 0.001
Thr	4.683ThrAla: 4.683 ± 0.065	0.599ThrCys: 0.599 ± 0.023	2.17ThrAsp: 2.17 ± 0.047	2.836ThrGlu: 2.836 ± 0.054	1.881ThrPhe: 1.881 ± 0.043	5.809ThrGly: 5.809 ± 0.065	0.96ThrHis: 0.96 ± 0.026	2.912ThrIle: 2.912 ± 0.048	1.847ThrLys: 1.847 ± 0.037	5.116ThrLeu: 5.116 ± 0.068	1.242ThrMet: 1.242 ± 0.031	1.468ThrAsn: 1.468 ± 0.034	2.763ThrPro: 2.763 ± 0.052	1.348ThrGln: 1.348 ± 0.033	3.222ThrArg: 3.222 ± 0.052	2.626ThrSer: 2.626 ± 0.041	2.605ThrThr: 2.605 ± 0.049	4.775ThrVal: 4.775 ± 0.065	0.605ThrTrp: 0.605 ± 0.023	1.47ThrTyr: 1.47 ± 0.035	0.002ThrXaa: 0.002 ± 0.001
Val	8.339ValAla: 8.339 ± 0.09	0.771ValCys: 0.771 ± 0.026	4.449ValAsp: 4.449 ± 0.049	5.909ValGlu: 5.909 ± 0.082	3.205ValPhe: 3.205 ± 0.054	6.219ValGly: 6.219 ± 0.075	1.417ValHis: 1.417 ± 0.036	5.217ValIle: 5.217 ± 0.071	4.053ValLys: 4.053 ± 0.063	8.921ValLeu: 8.921 ± 0.104	2.336ValMet: 2.336 ± 0.042	2.735ValAsn: 2.735 ± 0.045	4.04ValPro: 4.04 ± 0.059	2.559ValGln: 2.559 ± 0.044	4.843ValArg: 4.843 ± 0.063	4.505ValSer: 4.505 ± 0.068	4.981ValThr: 4.981 ± 0.075	7.851ValVal: 7.851 ± 0.089	0.788ValTrp: 0.788 ± 0.029	2.307ValTyr: 2.307 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.889TrpAla: 0.889 ± 0.03	0.114TrpCys: 0.114 ± 0.01	0.545TrpAsp: 0.545 ± 0.023	0.896TrpGlu: 0.896 ± 0.03	0.419TrpPhe: 0.419 ± 0.019	0.976TrpGly: 0.976 ± 0.03	0.199TrpHis: 0.199 ± 0.012	0.526TrpIle: 0.526 ± 0.023	0.47TrpLys: 0.47 ± 0.02	1.304TrpLeu: 1.304 ± 0.036	0.302TrpMet: 0.302 ± 0.014	0.367TrpAsn: 0.367 ± 0.018	0.468TrpPro: 0.468 ± 0.022	0.575TrpGln: 0.575 ± 0.02	0.777TrpArg: 0.777 ± 0.023	0.581TrpSer: 0.581 ± 0.023	0.492TrpThr: 0.492 ± 0.018	0.85TrpVal: 0.85 ± 0.027	0.221TrpTrp: 0.221 ± 0.014	0.313TrpTyr: 0.313 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.233TyrAla: 2.233 ± 0.04	0.386TyrCys: 0.386 ± 0.018	1.462TyrAsp: 1.462 ± 0.04	1.571TyrGlu: 1.571 ± 0.036	1.242TyrPhe: 1.242 ± 0.033	2.529TyrGly: 2.529 ± 0.05	0.745TyrHis: 0.745 ± 0.025	1.54TyrIle: 1.54 ± 0.038	1.007TyrLys: 1.007 ± 0.033	3.659TyrLeu: 3.659 ± 0.054	0.6TyrMet: 0.6 ± 0.022	0.942TyrAsn: 0.942 ± 0.03	1.526TyrPro: 1.526 ± 0.04	1.272TyrGln: 1.272 ± 0.033	2.332TyrArg: 2.332 ± 0.045	1.488TyrSer: 1.488 ± 0.039	1.564TyrThr: 1.564 ± 0.039	1.903TyrVal: 1.903 ± 0.039	0.398TyrTrp: 0.398 ± 0.019	1.035TyrTyr: 1.035 ± 0.03	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.002XaaLys: 0.002 ± 0.001	0.002XaaLeu: 0.002 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.002XaaAsn: 0.002 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.002XaaSer: 0.002 ± 0.001	0.003XaaThr: 0.003 ± 0.001	0.002XaaVal: 0.002 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.052XaaXaa: 0.052 ± 0.017

Statistics based on 4255 proteins (1260418 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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