Beihai sobemo-like virus 17

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEE0|A0A1L3KEE0_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 17 OX=1922688 PE=4 SV=1
MM1 pKa = 7.6ISYY4 pKa = 8.49EE5 pKa = 4.07QLTGYY10 pKa = 10.4YY11 pKa = 8.67YY12 pKa = 10.75VASAVCSRR20 pKa = 11.84VLEE23 pKa = 4.41AFLEE27 pKa = 4.52AMLNWLLSPYY37 pKa = 10.17GRR39 pKa = 11.84GVTFFLILQLAVFSAVLAYY58 pKa = 10.6AFTYY62 pKa = 10.15YY63 pKa = 10.38AAKK66 pKa = 10.24RR67 pKa = 11.84VWTLVKK73 pKa = 10.09YY74 pKa = 10.3VVFIFLAPSYY84 pKa = 10.01YY85 pKa = 10.53VYY87 pKa = 10.85EE88 pKa = 4.35RR89 pKa = 11.84YY90 pKa = 9.78IKK92 pKa = 10.32GFEE95 pKa = 4.45LIYY98 pKa = 10.24PRR100 pKa = 11.84NLFVQEE106 pKa = 4.46SINYY110 pKa = 6.71LQRR113 pKa = 11.84VKK115 pKa = 10.79ALPQCQVILCTRR127 pKa = 11.84SDD129 pKa = 3.1EE130 pKa = 4.48SLFRR134 pKa = 11.84KK135 pKa = 9.77SYY137 pKa = 10.54RR138 pKa = 11.84VIGQAFFIGINQGGPDD154 pKa = 3.69TLMTAAHH161 pKa = 6.26NLVGQDD167 pKa = 2.86KK168 pKa = 10.33EE169 pKa = 4.4YY170 pKa = 10.84FITRR174 pKa = 11.84DD175 pKa = 3.2MKK177 pKa = 10.38TFHH180 pKa = 6.97PVLSNEE186 pKa = 4.06QDD188 pKa = 3.21SGSPVIDD195 pKa = 3.45VVLLKK200 pKa = 10.68VPQGTGTKK208 pKa = 9.92LRR210 pKa = 11.84AAPASTQCWCPNSSAVEE227 pKa = 4.35VYY229 pKa = 10.61AGGMAEE235 pKa = 4.0GGEE238 pKa = 4.32PVKK241 pKa = 11.0SVGQVTSIEE250 pKa = 4.22GPNGIFTLAHH260 pKa = 5.94SASTDD265 pKa = 3.19GGFSGAPLFQGNKK278 pKa = 7.27VVGMHH283 pKa = 7.07LASLQNQVNLGVWLWPILGEE303 pKa = 4.28VKK305 pKa = 10.26EE306 pKa = 4.15VTPRR310 pKa = 11.84GFGDD314 pKa = 4.33FSQEE318 pKa = 3.74SGEE321 pKa = 4.11FSLEE325 pKa = 3.78HH326 pKa = 7.64SEE328 pKa = 6.42DD329 pKa = 3.04GFAWMKK335 pKa = 10.65DD336 pKa = 3.46EE337 pKa = 5.06PPFEE341 pKa = 4.25LSVRR345 pKa = 11.84GDD347 pKa = 3.1RR348 pKa = 11.84AYY350 pKa = 11.34VRR352 pKa = 11.84TSDD355 pKa = 3.27GRR357 pKa = 11.84NYY359 pKa = 10.61NYY361 pKa = 9.44EE362 pKa = 4.04ATDD365 pKa = 2.89HH366 pKa = 6.6HH367 pKa = 7.08LRR369 pKa = 11.84LLRR372 pKa = 11.84SKK374 pKa = 10.67YY375 pKa = 8.56SQFRR379 pKa = 11.84KK380 pKa = 10.29SYY382 pKa = 11.26DD383 pKa = 3.25DD384 pKa = 5.33FIDD387 pKa = 3.76YY388 pKa = 10.8DD389 pKa = 3.74QVEE392 pKa = 4.33RR393 pKa = 11.84DD394 pKa = 4.07SNYY397 pKa = 8.4HH398 pKa = 4.81TAVGKK403 pKa = 10.09AWEE406 pKa = 4.08VDD408 pKa = 3.4EE409 pKa = 5.36PDD411 pKa = 3.64FDD413 pKa = 5.98EE414 pKa = 5.28VIQWEE419 pKa = 4.41NAKK422 pKa = 10.56KK423 pKa = 9.74MEE425 pKa = 4.52NEE427 pKa = 5.49SFLQWIKK434 pKa = 9.96QQIDD438 pKa = 3.56PKK440 pKa = 10.41NLVWEE445 pKa = 4.51SKK447 pKa = 9.38PRR449 pKa = 11.84PMEE452 pKa = 4.09NLEE455 pKa = 4.11PTHH458 pKa = 6.32VLTGLRR464 pKa = 11.84AFSRR468 pKa = 11.84ILHH471 pKa = 6.69DD472 pKa = 4.3ANEE475 pKa = 4.37AKK477 pKa = 10.59VAMEE481 pKa = 3.77MMCRR485 pKa = 11.84TGNLNLARR493 pKa = 11.84RR494 pKa = 11.84DD495 pKa = 3.63KK496 pKa = 10.84EE497 pKa = 4.53ASFQQAADD505 pKa = 3.56ASLLADD511 pKa = 3.84TMRR514 pKa = 11.84VISTKK519 pKa = 10.4LAEE522 pKa = 4.41ASDD525 pKa = 4.25LFEE528 pKa = 4.46QVVQKK533 pKa = 10.65SDD535 pKa = 2.97KK536 pKa = 10.96DD537 pKa = 3.63AAQSTNWANYY547 pKa = 6.73RR548 pKa = 11.84HH549 pKa = 6.23KK550 pKa = 10.3HH551 pKa = 5.84RR552 pKa = 11.84DD553 pKa = 3.63DD554 pKa = 3.73MPPPQAQTRR563 pKa = 11.84AHH565 pKa = 5.91VDD567 pKa = 2.41RR568 pKa = 11.84WLRR571 pKa = 11.84NIEE574 pKa = 3.94TQLIVLRR581 pKa = 11.84AVPKK585 pKa = 10.16RR586 pKa = 11.84QTPTSISQTAEE597 pKa = 3.68PQVVQEE603 pKa = 4.41RR604 pKa = 11.84LSDD607 pKa = 3.63LSDD610 pKa = 3.2MFEE613 pKa = 4.18TFGLNFEE620 pKa = 4.35EE621 pKa = 5.04TEE623 pKa = 4.28DD624 pKa = 3.98GQDD627 pKa = 3.42FRR629 pKa = 11.84LGAPQKK635 pKa = 10.94EE636 pKa = 4.61DD637 pKa = 3.49GAPDD641 pKa = 3.5NQKK644 pKa = 10.9AEE646 pKa = 4.47LSSQPSLCSEE656 pKa = 4.21EE657 pKa = 4.26QSSEE661 pKa = 4.14TIQKK665 pKa = 9.45KK666 pKa = 10.37KK667 pKa = 9.78KK668 pKa = 10.51VSWAPNLEE676 pKa = 4.51TPSEE680 pKa = 4.34TRR682 pKa = 11.84NSRR685 pKa = 11.84HH686 pKa = 5.34QNTSRR691 pKa = 11.84KK692 pKa = 9.23PSQEE696 pKa = 3.94KK697 pKa = 11.02NNMGGPRR704 pKa = 11.84KK705 pKa = 9.23PRR707 pKa = 11.84NPRR710 pKa = 11.84RR711 pKa = 11.84FEE713 pKa = 3.71KK714 pKa = 10.52RR715 pKa = 11.84SNFTQEE721 pKa = 4.62SMMPPDD727 pKa = 3.79NGVEE731 pKa = 3.98EE732 pKa = 4.31LLKK735 pKa = 10.35KK736 pKa = 10.95VSMAALKK743 pKa = 10.43PFFEE747 pKa = 4.35ATLRR751 pKa = 11.84QLDD754 pKa = 3.66NSQQ757 pKa = 2.85

Molecular weight: 85.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEE0|A0A1L3KEE0_9VIRU RNA-directed RNA polymerase OS=Beihai sobemo-like virus 17 OX=1922688 PE=4 SV=1
MM1 pKa = 8.28RR2 pKa = 11.84DD3 pKa = 3.82GTPMLDD9 pKa = 3.63PKK11 pKa = 10.72SCPGNPWVSLGSTNEE26 pKa = 3.79VVFGYY31 pKa = 10.67LPGFGYY37 pKa = 10.55QGDD40 pKa = 4.11RR41 pKa = 11.84VNDD44 pKa = 4.16LYY46 pKa = 11.26HH47 pKa = 7.09AVLNRR52 pKa = 11.84FKK54 pKa = 10.83QLEE57 pKa = 4.3TAPAMDD63 pKa = 5.76PIFPFIKK70 pKa = 10.26QEE72 pKa = 3.87PHH74 pKa = 6.65KK75 pKa = 9.78KK76 pKa = 8.34TKK78 pKa = 10.66LEE80 pKa = 3.96EE81 pKa = 3.99GRR83 pKa = 11.84VRR85 pKa = 11.84IISGVSATDD94 pKa = 3.37QIVADD99 pKa = 4.34LLFMPEE105 pKa = 4.83LQSQMQNTLYY115 pKa = 10.93NGIAIGWAPNVPSGIVAFNEE135 pKa = 3.99VTEE138 pKa = 4.95GFDD141 pKa = 3.13TCADD145 pKa = 3.41KK146 pKa = 11.38SSWDD150 pKa = 3.34WTVVLWMVNFACQFFVSRR168 pKa = 11.84ATGCHH173 pKa = 4.83EE174 pKa = 3.75VLKK177 pKa = 10.72NHH179 pKa = 6.72ILAMLGPKK187 pKa = 9.6KK188 pKa = 10.45FKK190 pKa = 10.59FRR192 pKa = 11.84GGVFRR197 pKa = 11.84TEE199 pKa = 4.32HH200 pKa = 6.55PGVMPSGWKK209 pKa = 8.0VTILLNSLWQKK220 pKa = 10.38ACHH223 pKa = 6.01IMCGSPGKK231 pKa = 9.73ILSMGDD237 pKa = 3.28DD238 pKa = 4.56TIQEE242 pKa = 4.4PVQDD246 pKa = 4.19SYY248 pKa = 10.39WDD250 pKa = 3.43KK251 pKa = 10.48MAKK254 pKa = 9.91LGPKK258 pKa = 9.46IKK260 pKa = 10.15EE261 pKa = 3.63IRR263 pKa = 11.84KK264 pKa = 9.88DD265 pKa = 3.6GVKK268 pKa = 9.83EE269 pKa = 3.92FCGVTIDD276 pKa = 3.46KK277 pKa = 10.78NGYY280 pKa = 8.0YY281 pKa = 9.55PVYY284 pKa = 8.35TAKK287 pKa = 10.4HH288 pKa = 6.08SYY290 pKa = 9.46VLHH293 pKa = 5.99HH294 pKa = 7.0LKK296 pKa = 10.22PAVAIDD302 pKa = 3.59TLRR305 pKa = 11.84SYY307 pKa = 8.92QTLYY311 pKa = 10.79AYY313 pKa = 10.24CPKK316 pKa = 10.05QLSAIRR322 pKa = 11.84RR323 pKa = 11.84WLRR326 pKa = 11.84EE327 pKa = 3.78LGRR330 pKa = 11.84ADD332 pKa = 5.86AILPDD337 pKa = 4.86DD338 pKa = 5.17DD339 pKa = 5.2LRR341 pKa = 11.84KK342 pKa = 9.7PPLGLGG348 pKa = 3.44

Molecular weight: 39.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1105

348

757

552.5

62.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.059 ± 0.609

1.357 ± 0.56

5.52 ± 0.306

6.425 ± 1.428

4.706 ± 0.235

6.425 ± 1.134

2.081 ± 0.3

3.982 ± 0.879

5.973 ± 0.72

8.507 ± 0.239

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.715 ± 0.265

4.253 ± 0.479

5.792 ± 0.657

5.339 ± 0.953

5.43 ± 0.666

7.059 ± 1.121

4.977 ± 0.055

6.968 ± 0.128

1.991 ± 0.354

3.439 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski