Cereal yellow dwarf virus RPV

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q65863|Q65863_9LUTE RNA-directed RNA polymerase (Fragment) OS=Cereal yellow dwarf virus RPV OX=2170100 PE=4 SV=1
VV1 pKa = 7.21DD2 pKa = 4.96AEE4 pKa = 4.77PGPSPGPSPDD14 pKa = 3.76PPPPPSPSPEE24 pKa = 3.7PAPAKK29 pKa = 9.1EE30 pKa = 3.96EE31 pKa = 4.0RR32 pKa = 11.84FIVYY36 pKa = 9.99SGVAHH41 pKa = 6.68TIISAQSTDD50 pKa = 2.88DD51 pKa = 3.97SIIVRR56 pKa = 11.84DD57 pKa = 3.68IPDD60 pKa = 2.93QRR62 pKa = 11.84FRR64 pKa = 11.84YY65 pKa = 9.42VEE67 pKa = 3.84NEE69 pKa = 3.31NFYY72 pKa = 10.01WFQIAAQWYY81 pKa = 9.07SNTNTKK87 pKa = 9.69AVPMFVFPVPIGEE100 pKa = 4.08WSVEE104 pKa = 3.66ISTEE108 pKa = 4.42GYY110 pKa = 9.58QATSSTTDD118 pKa = 3.54PNKK121 pKa = 10.51GRR123 pKa = 11.84IDD125 pKa = 3.54GLIAYY130 pKa = 7.63DD131 pKa = 3.86NSSEE135 pKa = 3.83GWNIGAGSNVTITNNKK151 pKa = 9.13ADD153 pKa = 3.8NSWKK157 pKa = 10.41YY158 pKa = 9.45GHH160 pKa = 7.74PDD162 pKa = 3.24LEE164 pKa = 4.45INSCHH169 pKa = 6.41FNQNQVLEE177 pKa = 4.13KK178 pKa = 10.75DD179 pKa = 4.3GIISFHH185 pKa = 6.15VKK187 pKa = 9.04ATEE190 pKa = 3.83KK191 pKa = 10.18EE192 pKa = 4.23ANFFLVAPPVQKK204 pKa = 9.64TSKK207 pKa = 9.54YY208 pKa = 9.66NYY210 pKa = 8.82AVSYY214 pKa = 8.92GAWTDD219 pKa = 2.96RR220 pKa = 11.84DD221 pKa = 3.91MEE223 pKa = 4.8FGLITVTLDD232 pKa = 3.13EE233 pKa = 4.77KK234 pKa = 10.74RR235 pKa = 11.84GSGSPTRR242 pKa = 11.84KK243 pKa = 9.56SLRR246 pKa = 11.84AGHH249 pKa = 6.75AGVTTTTDD257 pKa = 3.42LVALPEE263 pKa = 4.23MEE265 pKa = 3.91NSGIEE270 pKa = 4.11TSEE273 pKa = 4.26TPSAPVTSSKK283 pKa = 11.25APLPTVSDD291 pKa = 4.22SEE293 pKa = 5.19SEE295 pKa = 4.19DD296 pKa = 4.15DD297 pKa = 4.59PLSAAPDD304 pKa = 3.45VGFGGTRR311 pKa = 11.84LLIDD315 pKa = 3.93TDD317 pKa = 3.28IKK319 pKa = 10.14TIPDD323 pKa = 4.07PDD325 pKa = 3.62VADD328 pKa = 3.96AFVNSAHH335 pKa = 6.54VGVDD339 pKa = 2.97PWAEE343 pKa = 3.6VRR345 pKa = 11.84AFKK348 pKa = 10.48RR349 pKa = 11.84AQRR352 pKa = 11.84PPRR355 pKa = 11.84GPSSVASSSLSGGSLRR371 pKa = 11.84GSLRR375 pKa = 11.84PKK377 pKa = 10.28TEE379 pKa = 3.9DD380 pKa = 3.55PKK382 pKa = 11.36DD383 pKa = 3.65SSKK386 pKa = 11.25SKK388 pKa = 9.64SRR390 pKa = 11.84KK391 pKa = 7.92WSLGSLRR398 pKa = 4.35

Molecular weight: 43.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q89645|Q89645_9LUTE Serine protease OS=Cereal yellow dwarf virus RPV OX=2170100 PE=4 SV=1
MM1 pKa = 7.25KK2 pKa = 10.27SIYY5 pKa = 9.6FVCLLAFCCQFSSQEE20 pKa = 3.91NLTLGAVTLTSPFLTPNYY38 pKa = 9.88LFDD41 pKa = 5.76GGSQWGTSLPSPLMVTFDD59 pKa = 3.49RR60 pKa = 11.84TQNSASTCPPCQPALSTSSSYY81 pKa = 11.7SDD83 pKa = 3.95IIQVGWQKK91 pKa = 11.26LSLDD95 pKa = 3.73SKK97 pKa = 10.77SAFKK101 pKa = 10.67AAWSLLKK108 pKa = 10.62DD109 pKa = 3.41SCTTASRR116 pKa = 11.84ALKK119 pKa = 10.36AAFHH123 pKa = 6.73DD124 pKa = 4.55LVGKK128 pKa = 9.81ALWLLVLIWTGLLRR142 pKa = 11.84QLFSAVWSAITNYY155 pKa = 10.47SLPVCLLISLGIITSWLWKK174 pKa = 8.42ACRR177 pKa = 11.84WLFGTLPALLCITLVKK193 pKa = 10.72NIFRR197 pKa = 11.84ILTFKK202 pKa = 10.82RR203 pKa = 11.84FFNEE207 pKa = 4.1KK208 pKa = 8.7TVSGYY213 pKa = 10.89DD214 pKa = 3.36SYY216 pKa = 11.55SIPSTPPKK224 pKa = 10.01RR225 pKa = 11.84SVIMMRR231 pKa = 11.84RR232 pKa = 11.84QNKK235 pKa = 7.03EE236 pKa = 3.55HH237 pKa = 6.97IGYY240 pKa = 9.95ANCIRR245 pKa = 11.84LFDD248 pKa = 3.92GRR250 pKa = 11.84NAIVTVAHH258 pKa = 6.67NIEE261 pKa = 4.54EE262 pKa = 4.66GCSFYY267 pKa = 11.16SSRR270 pKa = 11.84TSGSIPITEE279 pKa = 3.72FRR281 pKa = 11.84VIFEE285 pKa = 4.51SKK287 pKa = 9.85TMDD290 pKa = 3.0IAILVGPINWEE301 pKa = 4.18SILGCKK307 pKa = 9.29GVHH310 pKa = 5.5FTTADD315 pKa = 3.59RR316 pKa = 11.84LAEE319 pKa = 4.4CPAALYY325 pKa = 11.03LLDD328 pKa = 5.86SDD330 pKa = 4.52GQWRR334 pKa = 11.84SNSAKK339 pKa = 10.13ICGHH343 pKa = 6.57FDD345 pKa = 2.86NFAQVLSNTKK355 pKa = 9.69VGHH358 pKa = 5.97SGAGYY363 pKa = 9.82FYY365 pKa = 11.05GKK367 pKa = 8.15TLVGLHH373 pKa = 6.6KK374 pKa = 10.39GHH376 pKa = 7.46PGKK379 pKa = 10.42DD380 pKa = 3.41FNFNLMAPLPGIPGLTSPQYY400 pKa = 10.78VVEE403 pKa = 4.26SDD405 pKa = 3.71PPQGLVFPEE414 pKa = 4.23EE415 pKa = 4.04VTEE418 pKa = 4.45SIEE421 pKa = 4.19AAIKK425 pKa = 9.36EE426 pKa = 3.77ATMYY430 pKa = 11.23KK431 pKa = 10.38NVFANRR437 pKa = 11.84GRR439 pKa = 11.84GAFKK443 pKa = 10.64SKK445 pKa = 10.81SGINWEE451 pKa = 4.64DD452 pKa = 3.62IEE454 pKa = 5.35DD455 pKa = 3.56EE456 pKa = 4.33SGNGKK461 pKa = 9.44AAASAVTNAAAANKK475 pKa = 9.58VIATPGVAKK484 pKa = 10.09SQKK487 pKa = 8.23KK488 pKa = 6.64TAVPSSPKK496 pKa = 10.39APQPPAASQPTSTTGSRR513 pKa = 11.84TPICPIATATSPDD526 pKa = 3.52TAVPTGPSQQEE537 pKa = 3.57IMNNIMNLLVQRR549 pKa = 11.84IDD551 pKa = 3.11MSKK554 pKa = 9.37IEE556 pKa = 4.35KK557 pKa = 10.58SIVDD561 pKa = 3.38QVANQALKK569 pKa = 10.45KK570 pKa = 9.95PRR572 pKa = 11.84GKK574 pKa = 10.25RR575 pKa = 11.84GSKK578 pKa = 9.59KK579 pKa = 10.01RR580 pKa = 11.84PAAGKK585 pKa = 9.92SSSPTSTPGTYY596 pKa = 8.5QHH598 pKa = 7.01PNKK601 pKa = 10.03KK602 pKa = 9.41SQASNRR608 pKa = 11.84LGNSPPSTTRR618 pKa = 11.84APAANQNGGEE628 pKa = 3.9NSVPNTLSWVRR639 pKa = 11.84KK640 pKa = 9.05LPVSGGPKK648 pKa = 9.69SGHH651 pKa = 6.36PLNN654 pKa = 4.74

Molecular weight: 70.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1941

256

654

485.3

53.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.676 ± 0.509

2.009 ± 0.543

4.688 ± 0.8

5.564 ± 0.956

4.122 ± 0.651

6.801 ± 0.206

1.958 ± 0.268

4.688 ± 0.565

4.946 ± 0.773

8.501 ± 1.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.443 ± 0.209

4.482 ± 0.366

6.698 ± 1.002

3.658 ± 0.449

5.77 ± 1.034

9.995 ± 0.895

6.182 ± 0.6

5.925 ± 0.295

2.164 ± 0.186

2.731 ± 0.425
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski