Georgenia satyanarayanai
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3558 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Y9C4N5|A0A2Y9C4N5_9MICO ATP synthase subunit a OS=Georgenia satyanarayanai OX=860221 GN=atpB PE=3 SV=1
MM1 pKa = 7.72 RR2 pKa = 11.84 RR3 pKa = 11.84 PGTTTTALACVVVLAACGGGTDD25 pKa = 5.13 DD26 pKa = 5.76 DD27 pKa = 4.6 AAAPAPSSSSPAEE40 pKa = 4.04 DD41 pKa = 3.65 VNPVIDD47 pKa = 5.97 DD48 pKa = 4.57 DD49 pKa = 5.57 FPDD52 pKa = 4.71 PDD54 pKa = 3.76 VLEE57 pKa = 4.34 VDD59 pKa = 3.71 GTYY62 pKa = 10.47 YY63 pKa = 11.06 AYY65 pKa = 8.92 GTNGNLRR72 pKa = 11.84 NVRR75 pKa = 11.84 VARR78 pKa = 11.84 STDD81 pKa = 3.45 LEE83 pKa = 4.13 EE84 pKa = 4.28 WEE86 pKa = 4.66 LLEE89 pKa = 5.87 DD90 pKa = 4.86 ALPDD94 pKa = 3.55 LPRR97 pKa = 11.84 WVIPGKK103 pKa = 8.03 TWAPEE108 pKa = 3.84 VTEE111 pKa = 5.24 LDD113 pKa = 3.81 DD114 pKa = 4.92 GSYY117 pKa = 11.46 ALYY120 pKa = 8.51 FTATNYY126 pKa = 10.61 DD127 pKa = 3.91 PYY129 pKa = 9.94 AQCVGVATADD139 pKa = 3.49 APEE142 pKa = 4.71 GPFTVVGEE150 pKa = 4.19 EE151 pKa = 4.01 MLVCPQEE158 pKa = 3.74 QGGAIDD164 pKa = 4.9 ASTFTDD170 pKa = 3.41 TDD172 pKa = 3.4 GTRR175 pKa = 11.84 YY176 pKa = 10.34 LLWKK180 pKa = 10.31 NDD182 pKa = 3.19 GNAVGVDD189 pKa = 3.55 TWLHH193 pKa = 6.1 LAPLAPDD200 pKa = 3.9 GLSLTGEE207 pKa = 4.28 PEE209 pKa = 3.96 RR210 pKa = 11.84 LLKK213 pKa = 9.92 QDD215 pKa = 3.7 QDD217 pKa = 3.79 WEE219 pKa = 4.45 GHH221 pKa = 5.32 LVEE224 pKa = 5.74 APTLVEE230 pKa = 3.96 RR231 pKa = 11.84 DD232 pKa = 3.35 GTYY235 pKa = 11.23 VLLYY239 pKa = 10.11 SANDD243 pKa = 3.63 YY244 pKa = 11.24 GGEE247 pKa = 4.05 QYY249 pKa = 11.5 AVGYY253 pKa = 8.08 ATADD257 pKa = 3.47 AVAGPYY263 pKa = 8.94 TKK265 pKa = 10.41 ADD267 pKa = 3.87 EE268 pKa = 4.64 PLLTTDD274 pKa = 3.1 ATDD277 pKa = 3.41 GRR279 pKa = 11.84 FIGPGGQDD287 pKa = 2.92 VVTAPDD293 pKa = 3.49 GTDD296 pKa = 2.43 RR297 pKa = 11.84 LVFHH301 pKa = 6.79 SWYY304 pKa = 10.68 GDD306 pKa = 3.11 TSYY309 pKa = 11.09 RR310 pKa = 11.84 GVNVLPLEE318 pKa = 4.39 WDD320 pKa = 3.34 DD321 pKa = 3.64 GRR323 pKa = 11.84 PVVVVPP329 pKa = 4.85
Molecular weight: 35.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.745
IPC_protein 3.77
Toseland 3.541
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.592
Grimsley 3.452
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.139
Thurlkill 3.592
EMBOSS 3.719
Sillero 3.897
Patrickios 1.011
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A2Y9AEU9|A0A2Y9AEU9_9MICO Protease HtpX homolog OS=Georgenia satyanarayanai OX=860221 GN=htpX PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.79 HH17 pKa = 5.67 RR18 pKa = 11.84 KK19 pKa = 8.31 LLRR22 pKa = 11.84 KK23 pKa = 7.78 TRR25 pKa = 11.84 HH26 pKa = 3.65 QRR28 pKa = 11.84 RR29 pKa = 11.84 NKK31 pKa = 9.78 KK32 pKa = 9.85
Molecular weight: 4.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3558
0
3558
1190508
24
2677
334.6
35.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.781 ± 0.068
0.532 ± 0.01
6.256 ± 0.043
6.222 ± 0.042
2.48 ± 0.024
9.44 ± 0.035
2.113 ± 0.021
3.038 ± 0.032
1.329 ± 0.024
10.438 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.715 ± 0.016
1.598 ± 0.023
5.878 ± 0.039
2.733 ± 0.021
7.752 ± 0.051
4.947 ± 0.026
6.45 ± 0.034
9.93 ± 0.042
1.497 ± 0.016
1.872 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here