Clostridium sp. CAG:798

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1270 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6X5W3|R6X5W3_9CLOT Uncharacterized protein OS=Clostridium sp. CAG:798 OX=1262841 GN=BN787_00975 PE=4 SV=1
MM1 pKa = 7.67CLFDD5 pKa = 4.63LTPCEE10 pKa = 4.24LVLLAASIAIAFSQDD25 pKa = 2.94FSKK28 pKa = 11.57EE29 pKa = 3.79EE30 pKa = 3.98LTILAAFFTALGDD43 pKa = 3.8NFALLSTT50 pKa = 4.64

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6YCM5|R6YCM5_9CLOT Regulatory protein RecX OS=Clostridium sp. CAG:798 OX=1262841 GN=BN787_00577 PE=3 SV=1
MM1 pKa = 7.31ARR3 pKa = 11.84IAGIDD8 pKa = 3.5LPRR11 pKa = 11.84EE12 pKa = 3.78KK13 pKa = 10.09RR14 pKa = 11.84VEE16 pKa = 3.65IGLTYY21 pKa = 10.06IYY23 pKa = 10.59GIGLASSQKK32 pKa = 9.65ILAKK36 pKa = 10.74AEE38 pKa = 4.06VNPDD42 pKa = 2.86IRR44 pKa = 11.84VKK46 pKa = 10.9DD47 pKa = 3.65LTDD50 pKa = 3.95DD51 pKa = 3.39QVQAIRR57 pKa = 11.84KK58 pKa = 8.93AMEE61 pKa = 4.7GYY63 pKa = 9.97KK64 pKa = 10.08VEE66 pKa = 4.28GDD68 pKa = 3.5LRR70 pKa = 11.84RR71 pKa = 11.84EE72 pKa = 3.56VALNIKK78 pKa = 9.97RR79 pKa = 11.84LTEE82 pKa = 3.76IGCYY86 pKa = 9.38RR87 pKa = 11.84GLRR90 pKa = 11.84HH91 pKa = 6.78RR92 pKa = 11.84KK93 pKa = 8.09GLPVRR98 pKa = 11.84GQRR101 pKa = 11.84TKK103 pKa = 10.45TNARR107 pKa = 11.84TRR109 pKa = 11.84KK110 pKa = 9.44GPRR113 pKa = 11.84KK114 pKa = 9.42LVSKK118 pKa = 10.33GKK120 pKa = 10.17KK121 pKa = 8.98

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1270

0

1270

385101

29

2642

303.2

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.052 ± 0.059

1.133 ± 0.028

5.281 ± 0.053

8.486 ± 0.098

3.791 ± 0.056

5.626 ± 0.08

1.168 ± 0.024

10.827 ± 0.092

10.099 ± 0.09

8.44 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.375 ± 0.032

7.089 ± 0.074

2.595 ± 0.037

2.804 ± 0.031

3.266 ± 0.053

5.393 ± 0.061

5.562 ± 0.105

5.818 ± 0.054

0.64 ± 0.02

4.555 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski