Agrilus planipennis (Emerald ash borer) (Agrilus marcopoli)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Elateriformia;

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18772 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7F5RLS0|A0A7F5RLS0_AGRPL apoptosis inhibitor 5 homolog OS=Agrilus planipennis OX=224129 GN=LOC112906595 PE=3 SV=1
MM1 pKa = 7.31NSLFKK6 pKa = 10.45FCAIVTVLLDD16 pKa = 3.87LSTYY20 pKa = 9.74NPRR23 pKa = 11.84GSNTYY28 pKa = 8.31NTDD31 pKa = 3.75RR32 pKa = 11.84IISTKK37 pKa = 9.68IDD39 pKa = 3.31AAQSILTTNLSFHH52 pKa = 7.03LATEE56 pKa = 4.32TTTLFSTTVSNTITSSTDD74 pKa = 3.02SFPDD78 pKa = 2.79RR79 pKa = 11.84STIVNTPEE87 pKa = 3.58THH89 pKa = 5.89LTTGSSEE96 pKa = 4.13EE97 pKa = 4.2SSTLNIIDD105 pKa = 5.39GYY107 pKa = 8.07TISYY111 pKa = 10.38APDD114 pKa = 3.8AEE116 pKa = 4.44LTTEE120 pKa = 4.03ITAEE124 pKa = 4.15TSATNAPDD132 pKa = 3.27RR133 pKa = 11.84STIPNRR139 pKa = 11.84PNTQLTTDD147 pKa = 3.41TTEE150 pKa = 3.77QSFTTNIPYY159 pKa = 10.7DD160 pKa = 3.6LTIPLSKK167 pKa = 10.83VKK169 pKa = 9.54LTTEE173 pKa = 4.76GIEE176 pKa = 4.01EE177 pKa = 5.55GYY179 pKa = 6.78TTTGYY184 pKa = 8.56STIPDD189 pKa = 3.59TSGTEE194 pKa = 3.97LPTEE198 pKa = 4.28STTGAITNTASNHH211 pKa = 4.47STLLSNSEE219 pKa = 4.17VEE221 pKa = 4.44LTTEE225 pKa = 4.07STTDD229 pKa = 3.18ASITNLSDD237 pKa = 3.14NLTISNTVGAEE248 pKa = 3.83IITEE252 pKa = 4.3GTVEE256 pKa = 4.05TSTTNVPNDD265 pKa = 3.56SASPNVPEE273 pKa = 4.09TSITSGTTQHH283 pKa = 6.94PFLTSIPDD291 pKa = 3.79DD292 pKa = 3.71STVSYY297 pKa = 10.39TPGAEE302 pKa = 3.98LTTEE306 pKa = 4.26GTKK309 pKa = 9.51DD310 pKa = 2.93TSTINLSDD318 pKa = 3.8NLTISSTTKK327 pKa = 10.67AKK329 pKa = 10.3IITEE333 pKa = 4.3GTVEE337 pKa = 4.05TSTTNVPNDD346 pKa = 3.56SASPNVPEE354 pKa = 4.31TSTTSGTTQHH364 pKa = 6.97PFLTSIPDD372 pKa = 3.79DD373 pKa = 3.71STVSYY378 pKa = 10.39TPGAEE383 pKa = 4.02LTTEE387 pKa = 4.27STTDD391 pKa = 2.96TYY393 pKa = 9.04TTNLSDD399 pKa = 4.87NLTIPNTAGAEE410 pKa = 4.03ITTEE414 pKa = 4.29GSAEE418 pKa = 4.01TSTTNVPKK426 pKa = 10.76DD427 pKa = 3.43STIPNAPEE435 pKa = 3.46IQMTLASTDD444 pKa = 3.32VHH446 pKa = 6.34STDD449 pKa = 3.64TDD451 pKa = 3.5HH452 pKa = 7.17GNSTISYY459 pKa = 7.93TPGVEE464 pKa = 3.96LTTEE468 pKa = 3.62ITTDD472 pKa = 3.1AFSTHH477 pKa = 6.87PSDD480 pKa = 5.23HH481 pKa = 5.99LTIPNTIEE489 pKa = 4.37AEE491 pKa = 3.78ITTEE495 pKa = 4.01VNGDD499 pKa = 3.6STISNMPVTEE509 pKa = 4.37LTTEE513 pKa = 4.12STTDD517 pKa = 2.99ASTTNLSDD525 pKa = 3.53NLTIPNTTGAEE536 pKa = 3.89ITTEE540 pKa = 4.27GSAEE544 pKa = 4.03TSTTNIPNDD553 pKa = 3.38STIPNISDD561 pKa = 2.81IQMALGSTDD570 pKa = 3.66EE571 pKa = 4.65PSTDD575 pKa = 3.44TVHH578 pKa = 6.9GDD580 pKa = 3.2STISYY585 pKa = 8.67TPGAEE590 pKa = 4.0LTTEE594 pKa = 4.19STTDD598 pKa = 2.86TSTINLSDD606 pKa = 3.68NLIIPNTTGAEE617 pKa = 3.88ITTEE621 pKa = 4.03DD622 pKa = 3.47SAEE625 pKa = 3.9TFMANVPDD633 pKa = 5.17DD634 pKa = 3.75STIANTPQIQMTLGSTDD651 pKa = 3.63EE652 pKa = 4.59PSTDD656 pKa = 3.38TVHH659 pKa = 7.41SEE661 pKa = 4.03TTISNTLGLKK671 pKa = 8.92LTTEE675 pKa = 4.37GVTYY679 pKa = 11.02ASTTNVSDD687 pKa = 2.62SWTISNTPEE696 pKa = 3.78TKK698 pKa = 10.63LITEE702 pKa = 4.34STEE705 pKa = 3.88KK706 pKa = 10.76CCTSNVPDD714 pKa = 4.04YY715 pKa = 10.86STISDD720 pKa = 3.48TSGIEE725 pKa = 4.05LPTEE729 pKa = 4.15STTLASVNNGPYY741 pKa = 9.96HH742 pKa = 5.67STVSSTPHH750 pKa = 7.43AEE752 pKa = 3.78FTTEE756 pKa = 3.08ITAEE760 pKa = 4.09VSTTNAPSHH769 pKa = 5.37STVLNTLNVEE779 pKa = 4.3LTTEE783 pKa = 4.15GAVDD787 pKa = 3.51VSTANVPDD795 pKa = 5.43FSSIPYY801 pKa = 9.35TSDD804 pKa = 3.22VEE806 pKa = 4.44MNTDD810 pKa = 3.26NTVDD814 pKa = 3.06ASTTNRR820 pKa = 11.84PDD822 pKa = 2.74HH823 pKa = 5.33STIRR827 pKa = 11.84NIPASQSTTDD837 pKa = 3.2GSEE840 pKa = 4.29EE841 pKa = 4.02VSTTYY846 pKa = 11.15VLDD849 pKa = 3.87HH850 pKa = 6.51SVVPSMPEE858 pKa = 3.44IDD860 pKa = 3.44WTTKK864 pKa = 5.48TTEE867 pKa = 3.71GALPIRR873 pKa = 11.84FSAKK877 pKa = 8.44VTTISYY883 pKa = 6.1TTKK886 pKa = 10.81SPMATMEE893 pKa = 4.38STFSSLDD900 pKa = 3.53NNEE903 pKa = 3.91LTTTLVVTSSSTLSTDD919 pKa = 2.86SSFEE923 pKa = 4.06SSSDD927 pKa = 3.6GPSTSIEE934 pKa = 4.27TTTSFSLDD942 pKa = 3.42AVPEE946 pKa = 4.12ASASIAAIVSAVFGRR961 pKa = 11.84CVVCYY966 pKa = 9.72LVILCVNN973 pKa = 4.18

Molecular weight: 102.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W4X2L2|A0A1W4X2L2_AGRPL LOW QUALITY PROTEIN: serine palmitoyltransferase 1 OS=Agrilus planipennis OX=224129 GN=LOC108740496 PE=4 SV=2
MM1 pKa = 7.44SAHH4 pKa = 6.1KK5 pKa = 9.54TFIIKK10 pKa = 10.29RR11 pKa = 11.84KK12 pKa = 8.65LAKK15 pKa = 9.89KK16 pKa = 10.19LKK18 pKa = 8.64QNRR21 pKa = 11.84PIPQWVRR28 pKa = 11.84MRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.89LKK50 pKa = 10.55LL51 pKa = 3.39

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13040

5732

18772

11197405

30

16983

596.5

67.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.483 ± 0.016

2.168 ± 0.063

5.302 ± 0.015

6.922 ± 0.028

3.885 ± 0.017

5.283 ± 0.021

2.364 ± 0.01

6.008 ± 0.015

7.099 ± 0.031

8.862 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.073 ± 0.012

5.692 ± 0.022

5.196 ± 0.036

4.335 ± 0.019

4.869 ± 0.016

8.303 ± 0.024

5.865 ± 0.015

6.066 ± 0.014

1.003 ± 0.008

3.217 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski