Desulfosoma caldarium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Syntrophobacterales; Syntrophobacteraceae; Desulfosoma

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2924 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N1UQS2|A0A3N1UQS2_9DELT SNF2 domain-containing protein OS=Desulfosoma caldarium OX=610254 GN=EDC27_2161 PE=4 SV=1
MM1 pKa = 6.54VTALPPSSFLYY12 pKa = 9.59WAGPGLGYY20 pKa = 10.82DD21 pKa = 3.5GVVLVRR27 pKa = 11.84TDD29 pKa = 3.09DD30 pKa = 3.77GYY32 pKa = 11.49YY33 pKa = 10.48GSGVLLYY40 pKa = 10.87DD41 pKa = 4.09GGAVLTAAHH50 pKa = 6.47LVARR54 pKa = 11.84NGLPGAVVDD63 pKa = 3.78QAEE66 pKa = 4.67VVFQTTDD73 pKa = 2.85GEE75 pKa = 4.3YY76 pKa = 10.42SYY78 pKa = 10.94ISQEE82 pKa = 3.48ISVPTSYY89 pKa = 11.24DD90 pKa = 3.45PEE92 pKa = 4.28NLNGDD97 pKa = 4.39LALIWLPSTAPVSAEE112 pKa = 3.57RR113 pKa = 11.84YY114 pKa = 7.85EE115 pKa = 4.44LYY117 pKa = 10.26RR118 pKa = 11.84SHH120 pKa = 8.22DD121 pKa = 3.81EE122 pKa = 3.7IGRR125 pKa = 11.84VMTLVGYY132 pKa = 7.47GVPGDD137 pKa = 4.08GFYY140 pKa = 11.43GMYY143 pKa = 9.69EE144 pKa = 3.97NWSGPPLRR152 pKa = 11.84LRR154 pKa = 11.84AWNRR158 pKa = 11.84ADD160 pKa = 4.17ADD162 pKa = 3.65AGTVKK167 pKa = 10.6EE168 pKa = 4.0GLGYY172 pKa = 11.25VMDD175 pKa = 4.75WDD177 pKa = 4.12PKK179 pKa = 10.57EE180 pKa = 4.99DD181 pKa = 3.73SQLVADD187 pKa = 5.13FDD189 pKa = 5.04DD190 pKa = 3.83GTMVHH195 pKa = 7.04DD196 pKa = 5.15AMGSLLGVWDD206 pKa = 4.9LGLGYY211 pKa = 10.13YY212 pKa = 10.3EE213 pKa = 5.02GMISSGDD220 pKa = 3.42SGGPAFIEE228 pKa = 4.49GKK230 pKa = 9.69VAGIATAHH238 pKa = 6.76ASLGFWNIWPDD249 pKa = 2.95VDD251 pKa = 3.45IFKK254 pKa = 10.78NSSFGEE260 pKa = 3.81MGFWQRR266 pKa = 11.84VSSYY270 pKa = 9.56QEE272 pKa = 3.69WIDD275 pKa = 3.35RR276 pKa = 11.84SLRR279 pKa = 11.84SHH281 pKa = 6.52YY282 pKa = 10.19PNAPSRR288 pKa = 11.84PEE290 pKa = 4.24DD291 pKa = 3.39VQKK294 pKa = 10.87RR295 pKa = 11.84VTEE298 pKa = 4.27GDD300 pKa = 2.91AGTTYY305 pKa = 10.99AYY307 pKa = 10.72FLLQFNGVRR316 pKa = 11.84SDD318 pKa = 3.4PNAWVSVDD326 pKa = 3.12YY327 pKa = 7.47EE328 pKa = 4.5TRR330 pKa = 11.84DD331 pKa = 3.47GTATAGLDD339 pKa = 3.55YY340 pKa = 11.19VPASGTLILYY350 pKa = 9.36PGEE353 pKa = 4.82DD354 pKa = 3.7YY355 pKa = 11.44AVIPVEE361 pKa = 4.37IIGDD365 pKa = 3.97TVPEE369 pKa = 4.13PDD371 pKa = 3.07EE372 pKa = 4.51TFFLDD377 pKa = 3.08VFNPVGGSFGEE388 pKa = 4.75GVTKK392 pKa = 9.37LTAMRR397 pKa = 11.84TIVDD401 pKa = 3.91DD402 pKa = 5.79DD403 pKa = 4.39VSFWAA408 pKa = 4.83

Molecular weight: 44.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N1VLD6|A0A3N1VLD6_9DELT Metallo-beta-lactamase family protein OS=Desulfosoma caldarium OX=610254 GN=EDC27_0957 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.03QPHH8 pKa = 5.72NLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 7.76RR14 pKa = 11.84THH16 pKa = 5.88GFLVRR21 pKa = 11.84MATKK25 pKa = 10.34SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.4GRR39 pKa = 11.84KK40 pKa = 8.55RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2924

0

2924

962980

29

2176

329.3

36.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.713 ± 0.052

1.367 ± 0.022

5.058 ± 0.038

6.537 ± 0.05

3.981 ± 0.032

7.57 ± 0.042

2.57 ± 0.02

4.8 ± 0.037

4.294 ± 0.036

10.825 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.548 ± 0.02

2.461 ± 0.022

5.111 ± 0.029

3.575 ± 0.026

7.338 ± 0.042

5.161 ± 0.03

4.839 ± 0.024

8.243 ± 0.04

1.409 ± 0.019

2.599 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski