Brevundimonas sp.
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2819 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A519IVU7|A0A519IVU7_9CAUL Uncharacterized protein OS=Brevundimonas sp. OX=1871086 GN=EON91_12070 PE=4 SV=1
MM1 pKa = 7.56 RR2 pKa = 11.84 RR3 pKa = 11.84 YY4 pKa = 10.85 LMALGAASLICIAPAAASAQSRR26 pKa = 11.84 IAVGDD31 pKa = 3.97 TVDD34 pKa = 3.43 GALDD38 pKa = 3.54 ARR40 pKa = 11.84 DD41 pKa = 3.81 ARR43 pKa = 11.84 LGDD46 pKa = 3.58 DD47 pKa = 4.45 SYY49 pKa = 12.0 YY50 pKa = 11.11 DD51 pKa = 3.87 CYY53 pKa = 11.06 LLRR56 pKa = 11.84 IDD58 pKa = 5.0 RR59 pKa = 11.84 PQSVAITLKK68 pKa = 10.72 SSAFDD73 pKa = 3.46 AYY75 pKa = 10.79 LGAGHH80 pKa = 7.11 GVTCGGEE87 pKa = 3.78 AAEE90 pKa = 4.72 TNDD93 pKa = 4.99 DD94 pKa = 4.16 GPSMGTDD101 pKa = 2.78 SYY103 pKa = 11.75 LALEE107 pKa = 5.01 LGQGEE112 pKa = 4.55 YY113 pKa = 10.45 FIRR116 pKa = 11.84 ANSLSADD123 pKa = 3.23 KK124 pKa = 10.86 TGGYY128 pKa = 7.14 TLSIAPIQGGSDD140 pKa = 3.67 DD141 pKa = 4.5 NVTTLEE147 pKa = 4.13 GRR149 pKa = 11.84 LGQDD153 pKa = 3.42 DD154 pKa = 4.59 RR155 pKa = 11.84 KK156 pKa = 10.53 LADD159 pKa = 3.44 GSYY162 pKa = 9.77 YY163 pKa = 10.95 DD164 pKa = 4.13 CVDD167 pKa = 2.8 VRR169 pKa = 11.84 GRR171 pKa = 11.84 EE172 pKa = 4.34 GQQLTAGMEE181 pKa = 4.14 SEE183 pKa = 6.06 DD184 pKa = 3.91 FDD186 pKa = 3.97 TYY188 pKa = 11.44 LAILEE193 pKa = 4.55 GGGCQGSSLGTNDD206 pKa = 5.43 DD207 pKa = 3.95 GDD209 pKa = 4.42 DD210 pKa = 4.18 LGTDD214 pKa = 3.2 SLLAVTLPSDD224 pKa = 3.59 GVYY227 pKa = 10.43 SIRR230 pKa = 11.84 ANSLSANQTGAYY242 pKa = 8.63 LLHH245 pKa = 6.52 YY246 pKa = 10.64 ALDD249 pKa = 3.75
Molecular weight: 25.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.923
IPC_protein 3.948
Toseland 3.706
ProMoST 4.088
Dawson 3.948
Bjellqvist 4.126
Wikipedia 3.91
Rodwell 3.77
Grimsley 3.617
Solomon 3.948
Lehninger 3.897
Nozaki 4.062
DTASelect 4.355
Thurlkill 3.77
EMBOSS 3.91
Sillero 4.075
Patrickios 0.973
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.959
Protein with the highest isoelectric point:
>tr|A0A2D8ID82|A0A2D8ID82_9CAUL Acyl carrier protein OS=Brevundimonas sp. OX=1871086 GN=acpP PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 8.99 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.7 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.2 NGQKK29 pKa = 9.53 IVARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.77 GRR39 pKa = 11.84 KK40 pKa = 9.04 RR41 pKa = 11.84 LTAA44 pKa = 4.18
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.449
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.135
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.082
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2819
0
2819
847357
30
1629
300.6
32.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.96 ± 0.074
0.71 ± 0.011
6.137 ± 0.037
5.514 ± 0.042
3.402 ± 0.026
9.073 ± 0.046
1.793 ± 0.022
4.398 ± 0.031
2.697 ± 0.04
9.876 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.344 ± 0.021
2.287 ± 0.024
5.442 ± 0.034
3.169 ± 0.027
7.612 ± 0.049
4.914 ± 0.033
5.434 ± 0.036
7.712 ± 0.034
1.474 ± 0.021
2.053 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here