Escherichia phage nieznany

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phapecoctavirus; unclassified Phapecoctavirus

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9WXJ4|A0A6B9WXJ4_9CAUD Uncharacterized protein OS=Escherichia phage nieznany OX=2696432 GN=nieznany_53 PE=4 SV=1
MM1 pKa = 7.65GIDD4 pKa = 3.39IEE6 pKa = 4.21WRR8 pKa = 11.84MLQGAPYY15 pKa = 9.73DD16 pKa = 3.2KK17 pKa = 10.03WKK19 pKa = 10.67EE20 pKa = 3.89IFGDD24 pKa = 3.87DD25 pKa = 4.27PQVEE29 pKa = 4.27DD30 pKa = 4.98QFDD33 pKa = 3.8GDD35 pKa = 4.92FNWFLCEE42 pKa = 4.46GLGLEE47 pKa = 5.3NISPWYY53 pKa = 10.19DD54 pKa = 3.71ADD56 pKa = 4.77PEE58 pKa = 4.43DD59 pKa = 4.72CVFGVKK65 pKa = 10.16LCSGCGACAIDD76 pKa = 4.27LTSLNKK82 pKa = 9.61KK83 pKa = 9.98ANEE86 pKa = 4.0IYY88 pKa = 10.64FEE90 pKa = 4.74LLNKK94 pKa = 10.12YY95 pKa = 10.34NIEE98 pKa = 4.07TEE100 pKa = 4.32TLVSQNVWW108 pKa = 2.84

Molecular weight: 12.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9X0F0|A0A6B9X0F0_9CAUD Uncharacterized protein OS=Escherichia phage nieznany OX=2696432 GN=nieznany_117 PE=4 SV=1
MM1 pKa = 7.3NPKK4 pKa = 10.34DD5 pKa = 3.41MQAWKK10 pKa = 10.54GYY12 pKa = 10.53DD13 pKa = 3.44DD14 pKa = 6.35LSALRR19 pKa = 11.84NKK21 pKa = 10.17SSRR24 pKa = 11.84NLRR27 pKa = 11.84RR28 pKa = 11.84LEE30 pKa = 3.96AKK32 pKa = 9.82KK33 pKa = 10.45KK34 pKa = 10.39KK35 pKa = 9.97KK36 pKa = 10.33

Molecular weight: 4.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

256

0

256

43776

29

1189

171.0

19.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.901 ± 0.159

1.464 ± 0.09

6.357 ± 0.108

7.461 ± 0.204

4.413 ± 0.108

6.755 ± 0.199

1.919 ± 0.094

6.186 ± 0.117

6.81 ± 0.2

7.968 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.789 ± 0.079

5.339 ± 0.148

3.522 ± 0.102

3.132 ± 0.108

4.055 ± 0.141

5.857 ± 0.119

5.978 ± 0.227

6.976 ± 0.175

1.604 ± 0.081

4.514 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski