Amino acid dipepetide frequency for Streptomyces phage Zeigle

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.454AlaAla: 10.454 ± 1.009
0.619AlaCys: 0.619 ± 0.237
4.956AlaAsp: 4.956 ± 0.859
6.505AlaGlu: 6.505 ± 0.699
2.555AlaPhe: 2.555 ± 0.378
8.44AlaGly: 8.44 ± 0.912
1.394AlaHis: 1.394 ± 0.294
3.562AlaIle: 3.562 ± 0.657
4.724AlaLys: 4.724 ± 0.598
6.969AlaLeu: 6.969 ± 0.825
2.865AlaMet: 2.865 ± 0.442
3.949AlaAsn: 3.949 ± 0.494
3.949AlaPro: 3.949 ± 0.639
4.801AlaGln: 4.801 ± 0.98
4.878AlaArg: 4.878 ± 0.627
6.195AlaSer: 6.195 ± 0.607
6.814AlaThr: 6.814 ± 0.947
6.117AlaVal: 6.117 ± 0.801
2.246AlaTrp: 2.246 ± 0.366
4.104AlaTyr: 4.104 ± 0.571
0.0AlaXaa: 0.0 ± 0.0
Cys
0.31CysAla: 0.31 ± 0.145
0.0CysCys: 0.0 ± 0.0
0.465CysAsp: 0.465 ± 0.218
0.619CysGlu: 0.619 ± 0.208
0.155CysPhe: 0.155 ± 0.118
0.697CysGly: 0.697 ± 0.227
0.155CysHis: 0.155 ± 0.102
0.31CysIle: 0.31 ± 0.186
0.465CysLys: 0.465 ± 0.237
0.619CysLeu: 0.619 ± 0.285
0.155CysMet: 0.155 ± 0.109
0.077CysAsn: 0.077 ± 0.067
0.774CysPro: 0.774 ± 0.264
0.387CysGln: 0.387 ± 0.162
0.542CysArg: 0.542 ± 0.249
0.387CysSer: 0.387 ± 0.18
0.465CysThr: 0.465 ± 0.232
1.007CysVal: 1.007 ± 0.37
0.155CysTrp: 0.155 ± 0.11
0.077CysTyr: 0.077 ± 0.074
0.0CysXaa: 0.0 ± 0.0
Asp
7.047AspAla: 7.047 ± 0.699
0.697AspCys: 0.697 ± 0.292
4.414AspAsp: 4.414 ± 0.601
4.027AspGlu: 4.027 ± 0.623
2.246AspPhe: 2.246 ± 0.483
5.42AspGly: 5.42 ± 0.681
0.929AspHis: 0.929 ± 0.267
4.414AspIle: 4.414 ± 0.563
2.943AspLys: 2.943 ± 0.455
4.569AspLeu: 4.569 ± 0.543
2.478AspMet: 2.478 ± 0.605
1.936AspAsn: 1.936 ± 0.429
4.491AspPro: 4.491 ± 0.545
2.168AspGln: 2.168 ± 0.368
3.562AspArg: 3.562 ± 0.565
3.639AspSer: 3.639 ± 0.607
3.562AspThr: 3.562 ± 0.523
4.646AspVal: 4.646 ± 0.708
1.394AspTrp: 1.394 ± 0.299
2.478AspTyr: 2.478 ± 0.392
0.0AspXaa: 0.0 ± 0.0
Glu
5.963GluAla: 5.963 ± 0.678
0.542GluCys: 0.542 ± 0.307
3.562GluAsp: 3.562 ± 0.448
3.562GluGlu: 3.562 ± 0.517
2.013GluPhe: 2.013 ± 0.401
4.801GluGly: 4.801 ± 0.546
0.852GluHis: 0.852 ± 0.263
3.639GluIle: 3.639 ± 0.478
2.943GluLys: 2.943 ± 0.457
5.808GluLeu: 5.808 ± 0.663
1.626GluMet: 1.626 ± 0.329
2.013GluAsn: 2.013 ± 0.416
1.936GluPro: 1.936 ± 0.419
2.555GluGln: 2.555 ± 0.463
3.252GluArg: 3.252 ± 0.446
3.562GluSer: 3.562 ± 0.559
3.562GluThr: 3.562 ± 0.485
4.182GluVal: 4.182 ± 0.607
1.007GluTrp: 1.007 ± 0.272
3.02GluTyr: 3.02 ± 0.552
0.0GluXaa: 0.0 ± 0.0
Phe
3.02PheAla: 3.02 ± 0.673
0.155PheCys: 0.155 ± 0.112
2.71PheAsp: 2.71 ± 0.438
2.323PheGlu: 2.323 ± 0.444
1.084PhePhe: 1.084 ± 0.249
2.865PheGly: 2.865 ± 0.544
0.619PheHis: 0.619 ± 0.2
1.316PheIle: 1.316 ± 0.249
1.239PheLys: 1.239 ± 0.336
2.013PheLeu: 2.013 ± 0.532
0.697PheMet: 0.697 ± 0.248
1.626PheAsn: 1.626 ± 0.285
1.858PhePro: 1.858 ± 0.379
1.781PheGln: 1.781 ± 0.361
1.781PheArg: 1.781 ± 0.441
2.246PheSer: 2.246 ± 0.43
2.246PheThr: 2.246 ± 0.351
1.704PheVal: 1.704 ± 0.373
0.232PheTrp: 0.232 ± 0.137
1.394PheTyr: 1.394 ± 0.308
0.0PheXaa: 0.0 ± 0.0
Gly
8.131GlyAla: 8.131 ± 0.749
0.929GlyCys: 0.929 ± 0.315
5.188GlyAsp: 5.188 ± 0.707
5.808GlyGlu: 5.808 ± 0.589
3.33GlyPhe: 3.33 ± 0.568
8.982GlyGly: 8.982 ± 1.266
1.316GlyHis: 1.316 ± 0.379
3.717GlyIle: 3.717 ± 0.673
5.575GlyLys: 5.575 ± 0.692
6.272GlyLeu: 6.272 ± 0.826
2.633GlyMet: 2.633 ± 0.354
3.02GlyAsn: 3.02 ± 0.562
2.788GlyPro: 2.788 ± 0.341
4.104GlyGln: 4.104 ± 0.569
3.717GlyArg: 3.717 ± 0.506
6.737GlySer: 6.737 ± 0.716
5.963GlyThr: 5.963 ± 0.873
5.188GlyVal: 5.188 ± 0.599
1.471GlyTrp: 1.471 ± 0.309
3.485GlyTyr: 3.485 ± 0.7
0.0GlyXaa: 0.0 ± 0.0
His
1.162HisAla: 1.162 ± 0.23
0.155HisCys: 0.155 ± 0.11
1.239HisAsp: 1.239 ± 0.295
0.697HisGlu: 0.697 ± 0.215
0.619HisPhe: 0.619 ± 0.183
1.781HisGly: 1.781 ± 0.455
0.31HisHis: 0.31 ± 0.166
1.007HisIle: 1.007 ± 0.328
0.31HisLys: 0.31 ± 0.174
1.007HisLeu: 1.007 ± 0.249
0.387HisMet: 0.387 ± 0.149
0.619HisAsn: 0.619 ± 0.241
0.542HisPro: 0.542 ± 0.23
0.542HisGln: 0.542 ± 0.247
1.007HisArg: 1.007 ± 0.253
1.007HisSer: 1.007 ± 0.285
1.084HisThr: 1.084 ± 0.297
1.316HisVal: 1.316 ± 0.314
0.232HisTrp: 0.232 ± 0.173
0.852HisTyr: 0.852 ± 0.3
0.0HisXaa: 0.0 ± 0.0
Ile
2.865IleAla: 2.865 ± 0.435
0.465IleCys: 0.465 ± 0.23
3.872IleAsp: 3.872 ± 0.666
2.71IleGlu: 2.71 ± 0.431
1.162IlePhe: 1.162 ± 0.331
3.485IleGly: 3.485 ± 0.393
1.316IleHis: 1.316 ± 0.293
2.168IleIle: 2.168 ± 0.403
3.639IleLys: 3.639 ± 0.72
3.562IleLeu: 3.562 ± 0.474
1.239IleMet: 1.239 ± 0.368
2.168IleAsn: 2.168 ± 0.376
1.858IlePro: 1.858 ± 0.396
1.471IleGln: 1.471 ± 0.34
2.865IleArg: 2.865 ± 0.462
2.091IleSer: 2.091 ± 0.39
3.717IleThr: 3.717 ± 0.61
2.788IleVal: 2.788 ± 0.389
0.387IleTrp: 0.387 ± 0.144
1.239IleTyr: 1.239 ± 0.298
0.0IleXaa: 0.0 ± 0.0
Lys
5.885LysAla: 5.885 ± 0.827
0.542LysCys: 0.542 ± 0.249
3.407LysAsp: 3.407 ± 0.567
2.633LysGlu: 2.633 ± 0.478
2.4LysPhe: 2.4 ± 0.467
4.336LysGly: 4.336 ± 0.698
1.007LysHis: 1.007 ± 0.238
2.246LysIle: 2.246 ± 0.318
3.794LysLys: 3.794 ± 0.553
4.646LysLeu: 4.646 ± 0.638
1.781LysMet: 1.781 ± 0.308
2.633LysAsn: 2.633 ± 0.545
2.633LysPro: 2.633 ± 0.437
2.013LysGln: 2.013 ± 0.4
2.865LysArg: 2.865 ± 0.488
3.097LysSer: 3.097 ± 0.439
2.71LysThr: 2.71 ± 0.423
4.878LysVal: 4.878 ± 0.638
1.084LysTrp: 1.084 ± 0.249
1.858LysTyr: 1.858 ± 0.473
0.0LysXaa: 0.0 ± 0.0
Leu
5.808LeuAla: 5.808 ± 0.915
0.31LeuCys: 0.31 ± 0.186
5.111LeuAsp: 5.111 ± 0.738
3.794LeuGlu: 3.794 ± 0.51
2.71LeuPhe: 2.71 ± 0.474
5.111LeuGly: 5.111 ± 0.559
0.929LeuHis: 0.929 ± 0.307
3.562LeuIle: 3.562 ± 0.496
4.336LeuLys: 4.336 ± 0.638
4.336LeuLeu: 4.336 ± 0.576
1.316LeuMet: 1.316 ± 0.35
2.4LeuAsn: 2.4 ± 0.469
2.633LeuPro: 2.633 ± 0.385
4.336LeuGln: 4.336 ± 0.682
4.956LeuArg: 4.956 ± 0.64
5.42LeuSer: 5.42 ± 0.652
3.717LeuThr: 3.717 ± 0.568
5.033LeuVal: 5.033 ± 0.592
0.929LeuTrp: 0.929 ± 0.348
2.633LeuTyr: 2.633 ± 0.387
0.0LeuXaa: 0.0 ± 0.0
Met
4.491MetAla: 4.491 ± 0.584
0.155MetCys: 0.155 ± 0.108
2.168MetAsp: 2.168 ± 0.384
1.781MetGlu: 1.781 ± 0.405
0.619MetPhe: 0.619 ± 0.17
3.175MetGly: 3.175 ± 0.499
0.232MetHis: 0.232 ± 0.128
1.239MetIle: 1.239 ± 0.262
1.549MetLys: 1.549 ± 0.402
1.084MetLeu: 1.084 ± 0.361
0.929MetMet: 0.929 ± 0.293
1.239MetAsn: 1.239 ± 0.317
1.626MetPro: 1.626 ± 0.397
0.774MetGln: 0.774 ± 0.285
1.471MetArg: 1.471 ± 0.304
2.168MetSer: 2.168 ± 0.415
1.936MetThr: 1.936 ± 0.425
1.704MetVal: 1.704 ± 0.351
0.387MetTrp: 0.387 ± 0.15
0.155MetTyr: 0.155 ± 0.1
0.0MetXaa: 0.0 ± 0.0
Asn
3.717AsnAla: 3.717 ± 0.569
0.465AsnCys: 0.465 ± 0.176
2.091AsnAsp: 2.091 ± 0.358
1.936AsnGlu: 1.936 ± 0.334
1.239AsnPhe: 1.239 ± 0.262
3.639AsnGly: 3.639 ± 0.618
0.542AsnHis: 0.542 ± 0.198
1.471AsnIle: 1.471 ± 0.342
2.323AsnLys: 2.323 ± 0.498
2.943AsnLeu: 2.943 ± 0.474
0.852AsnMet: 0.852 ± 0.257
0.929AsnAsn: 0.929 ± 0.237
2.633AsnPro: 2.633 ± 0.459
1.858AsnGln: 1.858 ± 0.365
1.936AsnArg: 1.936 ± 0.421
1.781AsnSer: 1.781 ± 0.401
2.4AsnThr: 2.4 ± 0.304
1.781AsnVal: 1.781 ± 0.4
0.774AsnTrp: 0.774 ± 0.232
1.394AsnTyr: 1.394 ± 0.322
0.0AsnXaa: 0.0 ± 0.0
Pro
4.646ProAla: 4.646 ± 0.52
0.465ProCys: 0.465 ± 0.237
3.252ProAsp: 3.252 ± 0.413
2.865ProGlu: 2.865 ± 0.496
1.626ProPhe: 1.626 ± 0.328
4.724ProGly: 4.724 ± 0.636
0.465ProHis: 0.465 ± 0.195
1.549ProIle: 1.549 ± 0.478
2.246ProLys: 2.246 ± 0.428
2.71ProLeu: 2.71 ± 0.384
1.162ProMet: 1.162 ± 0.351
1.394ProAsn: 1.394 ± 0.316
2.013ProPro: 2.013 ± 0.529
1.162ProGln: 1.162 ± 0.352
2.168ProArg: 2.168 ± 0.47
2.555ProSer: 2.555 ± 0.516
3.485ProThr: 3.485 ± 0.451
3.097ProVal: 3.097 ± 0.453
0.619ProTrp: 0.619 ± 0.22
1.704ProTyr: 1.704 ± 0.367
0.0ProXaa: 0.0 ± 0.0
Gln
4.878GlnAla: 4.878 ± 0.712
0.232GlnCys: 0.232 ± 0.142
2.478GlnAsp: 2.478 ± 0.344
2.71GlnGlu: 2.71 ± 0.544
1.394GlnPhe: 1.394 ± 0.344
3.02GlnGly: 3.02 ± 0.514
0.774GlnHis: 0.774 ± 0.22
1.858GlnIle: 1.858 ± 0.367
2.246GlnLys: 2.246 ± 0.479
3.33GlnLeu: 3.33 ± 0.574
1.626GlnMet: 1.626 ± 0.467
1.084GlnAsn: 1.084 ± 0.345
2.013GlnPro: 2.013 ± 0.44
2.633GlnGln: 2.633 ± 0.596
3.562GlnArg: 3.562 ± 0.797
2.323GlnSer: 2.323 ± 0.31
2.865GlnThr: 2.865 ± 0.452
3.02GlnVal: 3.02 ± 0.518
0.929GlnTrp: 0.929 ± 0.249
1.781GlnTyr: 1.781 ± 0.364
0.0GlnXaa: 0.0 ± 0.0
Arg
5.963ArgAla: 5.963 ± 0.822
0.542ArgCys: 0.542 ± 0.226
4.104ArgAsp: 4.104 ± 0.506
3.562ArgGlu: 3.562 ± 0.615
1.704ArgPhe: 1.704 ± 0.466
4.569ArgGly: 4.569 ± 0.412
1.162ArgHis: 1.162 ± 0.322
2.168ArgIle: 2.168 ± 0.373
2.71ArgLys: 2.71 ± 0.525
3.794ArgLeu: 3.794 ± 0.568
2.091ArgMet: 2.091 ± 0.429
1.781ArgAsn: 1.781 ± 0.433
1.471ArgPro: 1.471 ± 0.264
2.4ArgGln: 2.4 ± 0.489
2.478ArgArg: 2.478 ± 0.465
3.33ArgSer: 3.33 ± 0.619
3.562ArgThr: 3.562 ± 0.414
4.336ArgVal: 4.336 ± 0.638
0.852ArgTrp: 0.852 ± 0.265
1.316ArgTyr: 1.316 ± 0.277
0.0ArgXaa: 0.0 ± 0.0
Ser
5.42SerAla: 5.42 ± 0.721
0.387SerCys: 0.387 ± 0.235
4.491SerAsp: 4.491 ± 0.691
4.414SerGlu: 4.414 ± 0.694
1.394SerPhe: 1.394 ± 0.326
7.047SerGly: 7.047 ± 1.139
1.007SerHis: 1.007 ± 0.253
3.175SerIle: 3.175 ± 0.376
2.865SerLys: 2.865 ± 0.546
3.639SerLeu: 3.639 ± 0.508
1.394SerMet: 1.394 ± 0.293
3.02SerAsn: 3.02 ± 0.497
2.478SerPro: 2.478 ± 0.451
2.4SerGln: 2.4 ± 0.327
3.175SerArg: 3.175 ± 0.405
4.956SerSer: 4.956 ± 0.974
3.562SerThr: 3.562 ± 0.561
4.801SerVal: 4.801 ± 0.564
1.162SerTrp: 1.162 ± 0.301
2.633SerTyr: 2.633 ± 0.426
0.0SerXaa: 0.0 ± 0.0
Thr
5.266ThrAla: 5.266 ± 0.85
0.31ThrCys: 0.31 ± 0.183
4.182ThrAsp: 4.182 ± 0.594
2.943ThrGlu: 2.943 ± 0.435
2.71ThrPhe: 2.71 ± 0.343
6.427ThrGly: 6.427 ± 1.043
1.162ThrHis: 1.162 ± 0.328
3.252ThrIle: 3.252 ± 0.481
4.336ThrLys: 4.336 ± 0.832
4.646ThrLeu: 4.646 ± 0.648
2.091ThrMet: 2.091 ± 0.42
1.936ThrAsn: 1.936 ± 0.481
4.259ThrPro: 4.259 ± 0.557
3.252ThrGln: 3.252 ± 0.452
2.323ThrArg: 2.323 ± 0.318
3.717ThrSer: 3.717 ± 0.841
5.033ThrThr: 5.033 ± 0.911
4.569ThrVal: 4.569 ± 0.672
1.239ThrTrp: 1.239 ± 0.273
2.246ThrTyr: 2.246 ± 0.385
0.0ThrXaa: 0.0 ± 0.0
Val
5.963ValAla: 5.963 ± 0.676
0.542ValCys: 0.542 ± 0.228
5.885ValAsp: 5.885 ± 0.685
3.717ValGlu: 3.717 ± 0.635
2.168ValPhe: 2.168 ± 0.372
5.111ValGly: 5.111 ± 0.581
1.162ValHis: 1.162 ± 0.258
2.246ValIle: 2.246 ± 0.422
4.801ValLys: 4.801 ± 0.527
3.872ValLeu: 3.872 ± 0.476
2.091ValMet: 2.091 ± 0.317
2.555ValAsn: 2.555 ± 0.467
2.013ValPro: 2.013 ± 0.418
3.717ValGln: 3.717 ± 0.691
4.259ValArg: 4.259 ± 0.759
5.42ValSer: 5.42 ± 0.521
5.111ValThr: 5.111 ± 0.785
4.259ValVal: 4.259 ± 0.661
1.471ValTrp: 1.471 ± 0.302
2.555ValTyr: 2.555 ± 0.402
0.0ValXaa: 0.0 ± 0.0
Trp
1.471TrpAla: 1.471 ± 0.321
0.077TrpCys: 0.077 ± 0.089
1.239TrpAsp: 1.239 ± 0.314
1.394TrpGlu: 1.394 ± 0.38
0.697TrpPhe: 0.697 ± 0.195
0.929TrpGly: 0.929 ± 0.261
0.155TrpHis: 0.155 ± 0.096
0.619TrpIle: 0.619 ± 0.22
1.316TrpLys: 1.316 ± 0.309
1.239TrpLeu: 1.239 ± 0.31
0.465TrpMet: 0.465 ± 0.207
0.465TrpAsn: 0.465 ± 0.161
0.542TrpPro: 0.542 ± 0.237
0.774TrpGln: 0.774 ± 0.201
1.239TrpArg: 1.239 ± 0.303
0.774TrpSer: 0.774 ± 0.256
1.626TrpThr: 1.626 ± 0.349
1.316TrpVal: 1.316 ± 0.253
0.31TrpTrp: 0.31 ± 0.146
0.852TrpTyr: 0.852 ± 0.259
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.639TyrAla: 3.639 ± 0.528
0.232TyrCys: 0.232 ± 0.12
2.323TyrAsp: 2.323 ± 0.437
2.4TyrGlu: 2.4 ± 0.434
1.084TyrPhe: 1.084 ± 0.258
3.794TyrGly: 3.794 ± 0.463
0.387TyrHis: 0.387 ± 0.175
1.549TyrIle: 1.549 ± 0.311
2.091TyrLys: 2.091 ± 0.378
2.323TyrLeu: 2.323 ± 0.351
0.929TyrMet: 0.929 ± 0.311
1.858TyrAsn: 1.858 ± 0.397
1.394TyrPro: 1.394 ± 0.329
1.626TyrGln: 1.626 ± 0.31
1.858TyrArg: 1.858 ± 0.296
2.013TyrSer: 2.013 ± 0.346
2.478TyrThr: 2.478 ± 0.473
3.175TyrVal: 3.175 ± 0.439
0.619TyrTrp: 0.619 ± 0.211
0.929TyrTyr: 0.929 ± 0.204
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12915 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski