Vibrio maritimus
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6793 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A090T331|A0A090T331_9VIBR Transcriptional regulator MerR family OS=Vibrio maritimus OX=990268 GN=JCM19240_2269 PE=4 SV=1
MM1 pKa = 7.51 QITNLVDD8 pKa = 2.73 IWGNSHH14 pKa = 7.34 LYY16 pKa = 8.92 VQGDD20 pKa = 3.76 VSVGTNDD27 pKa = 3.96 DD28 pKa = 3.41 GRR30 pKa = 11.84 FTLFQNSAAQVGGNLSSGEE49 pKa = 4.17 MEE51 pKa = 4.7 CSNSSSFDD59 pKa = 3.08 VDD61 pKa = 3.53 GNLEE65 pKa = 4.3 TRR67 pKa = 11.84 GSLSWSGGQFGLAIQRR83 pKa = 11.84 GCFGRR88 pKa = 11.84 YY89 pKa = 9.67 GEE91 pKa = 4.25 TDD93 pKa = 3.2 SNNTLEE99 pKa = 4.43 GEE101 pKa = 4.39 YY102 pKa = 10.5 FIDD105 pKa = 3.44 QHH107 pKa = 7.65 SEE109 pKa = 4.09 VYY111 pKa = 9.29 TPSWDD116 pKa = 3.49 TLRR119 pKa = 11.84 EE120 pKa = 4.29 SNDD123 pKa = 3.38 EE124 pKa = 4.09 EE125 pKa = 4.42 PQQ127 pKa = 3.29
Molecular weight: 13.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.91
IPC_protein 3.859
Toseland 3.656
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.757
Rodwell 3.694
Grimsley 3.579
Solomon 3.821
Lehninger 3.77
Nozaki 3.961
DTASelect 4.139
Thurlkill 3.706
EMBOSS 3.77
Sillero 3.973
Patrickios 1.888
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.871
Protein with the highest isoelectric point:
>tr|A0A090SUZ0|A0A090SUZ0_9VIBR Uncharacterized protein OS=Vibrio maritimus OX=990268 GN=JCM19240_4996 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 8.98 RR4 pKa = 11.84 TFQPSVLKK12 pKa = 10.49 RR13 pKa = 11.84 KK14 pKa = 7.97 RR15 pKa = 11.84 THH17 pKa = 5.89 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.44 NGRR29 pKa = 11.84 KK30 pKa = 9.39 VINARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.22 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSKK45 pKa = 10.83
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6793
0
6793
1574031
37
3915
231.7
25.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.763 ± 0.032
1.011 ± 0.012
5.531 ± 0.038
6.273 ± 0.032
4.144 ± 0.021
6.927 ± 0.031
2.192 ± 0.018
6.179 ± 0.026
5.074 ± 0.029
10.153 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.84 ± 0.02
4.146 ± 0.022
3.865 ± 0.02
4.419 ± 0.023
4.415 ± 0.026
6.837 ± 0.03
5.598 ± 0.026
7.317 ± 0.025
1.285 ± 0.014
3.029 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here