Hubei rhabdo-like virus 9

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Betanemrhavirus; Hubei betanemrhavirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KN66|A0A1L3KN66_9VIRU Uncharacterized protein OS=Hubei rhabdo-like virus 9 OX=1923193 PE=4 SV=1
MM1 pKa = 6.78MTQCEE6 pKa = 4.6LVCLKK11 pKa = 10.28PITITVIGLLITAVKK26 pKa = 10.07SVQEE30 pKa = 3.86RR31 pKa = 11.84PRR33 pKa = 11.84YY34 pKa = 9.4LDD36 pKa = 3.48HH37 pKa = 6.69LTVSGPITRR46 pKa = 11.84QNVPVYY52 pKa = 9.25DD53 pKa = 4.31CKK55 pKa = 11.27VSGIGMKK62 pKa = 9.82TPIPPRR68 pKa = 11.84PKK70 pKa = 10.14CDD72 pKa = 3.08SLLPKK77 pKa = 10.76NPDD80 pKa = 3.23EE81 pKa = 4.03EE82 pKa = 4.47HH83 pKa = 6.47QLVSVPVQTCMIRR96 pKa = 11.84ILTCTCDD103 pKa = 3.5YY104 pKa = 11.69VEE106 pKa = 5.58DD107 pKa = 4.0LTSNSTVSYY116 pKa = 8.15EE117 pKa = 3.99YY118 pKa = 10.39CVPSLYY124 pKa = 10.63SISEE128 pKa = 4.32SEE130 pKa = 4.52CNATCSDD137 pKa = 3.92PTFQPPTSSGLDD149 pKa = 3.46CPHH152 pKa = 7.37PEE154 pKa = 4.31LVTEE158 pKa = 4.15YY159 pKa = 10.17PEE161 pKa = 4.71CFLTAAPFSRR171 pKa = 11.84ATGYY175 pKa = 10.7FFDD178 pKa = 3.55QEE180 pKa = 4.1EE181 pKa = 4.57RR182 pKa = 11.84FDD184 pKa = 3.51CHH186 pKa = 7.75YY187 pKa = 10.82LDD189 pKa = 4.23RR190 pKa = 11.84YY191 pKa = 10.38CRR193 pKa = 11.84VNEE196 pKa = 3.63YY197 pKa = 10.37RR198 pKa = 11.84ALRR201 pKa = 11.84WRR203 pKa = 11.84VEE205 pKa = 4.05QSWVLLL211 pKa = 3.96

Molecular weight: 24.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KN61|A0A1L3KN61_9VIRU Uncharacterized protein OS=Hubei rhabdo-like virus 9 OX=1923193 PE=4 SV=1
MM1 pKa = 7.56SSYY4 pKa = 11.31LVMSVEE10 pKa = 4.66GVVSTDD16 pKa = 3.83FEE18 pKa = 5.41ASLDD22 pKa = 3.8RR23 pKa = 11.84QIGLNRR29 pKa = 11.84LIHH32 pKa = 5.76EE33 pKa = 4.48VLVRR37 pKa = 11.84TTPRR41 pKa = 11.84IPEE44 pKa = 3.83WCRR47 pKa = 11.84IPVGCVVAYY56 pKa = 9.75IVDD59 pKa = 3.98RR60 pKa = 11.84SHH62 pKa = 5.84QTTEE66 pKa = 4.96PIPTPYY72 pKa = 10.17GEE74 pKa = 4.23KK75 pKa = 10.47VGFHH79 pKa = 6.27AVGLGAGGYY88 pKa = 10.02AVLHH92 pKa = 6.61DD93 pKa = 4.75SKK95 pKa = 11.27HH96 pKa = 6.44SIDD99 pKa = 3.51RR100 pKa = 11.84QHH102 pKa = 5.72FHH104 pKa = 7.02SVFEE108 pKa = 4.46DD109 pKa = 2.97ATMKK113 pKa = 11.1DD114 pKa = 3.19NTFQSMKK121 pKa = 10.73SSLKK125 pKa = 8.1WTLTVVDD132 pKa = 5.15GVTPANLVATGKK144 pKa = 10.38IILDD148 pKa = 3.58PRR150 pKa = 11.84VDD152 pKa = 3.68ADD154 pKa = 4.07RR155 pKa = 11.84LRR157 pKa = 11.84VLAKK161 pKa = 10.3LPSRR165 pKa = 11.84GVAGPSGLMKK175 pKa = 10.97LFTRR179 pKa = 11.84GNKK182 pKa = 8.97

Molecular weight: 19.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

4253

182

2217

607.6

68.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.019 ± 0.649

2.187 ± 0.405

5.337 ± 0.246

5.502 ± 0.488

4.021 ± 0.237

4.726 ± 0.363

2.445 ± 0.25

6.584 ± 0.692

5.29 ± 0.213

9.875 ± 0.517

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.412

3.597 ± 0.181

5.267 ± 0.48

3.692 ± 0.329

5.361 ± 0.324

9.476 ± 0.65

6.466 ± 0.332

6.372 ± 0.434

1.528 ± 0.207

3.527 ± 0.375
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski