Bacillus cohnii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4687 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A223KT44|A0A223KT44_9BACI Carbon starvation protein A OS=Bacillus cohnii OX=33932 GN=BC6307_14920 PE=3 SV=1
MM1 pKa = 7.5SLEE4 pKa = 3.75WFDD7 pKa = 4.23RR8 pKa = 11.84VTGEE12 pKa = 4.39LQDD15 pKa = 3.95NLEE18 pKa = 4.67SICDD22 pKa = 3.53KK23 pKa = 10.99YY24 pKa = 11.53DD25 pKa = 3.33QTGHH29 pKa = 5.27MSIEE33 pKa = 4.21RR34 pKa = 11.84GAKK37 pKa = 8.53HH38 pKa = 5.93PRR40 pKa = 11.84IEE42 pKa = 4.86FYY44 pKa = 10.83TEE46 pKa = 3.48IDD48 pKa = 3.83DD49 pKa = 4.24EE50 pKa = 4.61LEE52 pKa = 3.76RR53 pKa = 11.84DD54 pKa = 3.96YY55 pKa = 11.5FCTLFFDD62 pKa = 4.84PSNEE66 pKa = 3.8EE67 pKa = 4.4YY68 pKa = 11.46YY69 pKa = 9.8MEE71 pKa = 4.68TVDD74 pKa = 4.69PEE76 pKa = 4.22YY77 pKa = 11.42GHH79 pKa = 6.92LSKK82 pKa = 11.16VILPDD87 pKa = 3.21IEE89 pKa = 5.75DD90 pKa = 3.4IVDD93 pKa = 3.58AVHH96 pKa = 6.96EE97 pKa = 4.45SFHH100 pKa = 8.89DD101 pKa = 3.88YY102 pKa = 10.97MNGDD106 pKa = 3.89MNEE109 pKa = 3.68VSYY112 pKa = 10.75MDD114 pKa = 3.8NEE116 pKa = 5.26DD117 pKa = 3.48YY118 pKa = 11.18TEE120 pKa = 4.04TDD122 pKa = 2.82IVVYY126 pKa = 10.64DD127 pKa = 3.52SDD129 pKa = 4.11YY130 pKa = 11.9DD131 pKa = 3.73EE132 pKa = 5.71VEE134 pKa = 4.37NNDD137 pKa = 3.54QIYY140 pKa = 10.8EE141 pKa = 4.23EE142 pKa = 5.32IDD144 pKa = 3.87VEE146 pKa = 4.36WEE148 pKa = 4.22TPEE151 pKa = 3.57VTAYY155 pKa = 10.81YY156 pKa = 10.47EE157 pKa = 3.98EE158 pKa = 5.7DD159 pKa = 3.76EE160 pKa = 5.25VEE162 pKa = 3.97VTYY165 pKa = 10.95QFGVVQEE172 pKa = 4.44TGDD175 pKa = 3.51GVLRR179 pKa = 11.84RR180 pKa = 11.84INRR183 pKa = 11.84IRR185 pKa = 11.84TGDD188 pKa = 3.71DD189 pKa = 3.04EE190 pKa = 4.27MLKK193 pKa = 10.85DD194 pKa = 3.4EE195 pKa = 4.76TNFIFSKK202 pKa = 10.7EE203 pKa = 3.91EE204 pKa = 3.7AGTIIALIASHH215 pKa = 7.13LDD217 pKa = 3.43SMGDD221 pKa = 3.12TGDD224 pKa = 3.62YY225 pKa = 10.37FF226 pKa = 4.9

Molecular weight: 26.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A223KX24|A0A223KX24_9BACI Uncharacterized protein OS=Bacillus cohnii OX=33932 GN=BC6307_23425 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPNGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4687

0

4687

1336336

26

3035

285.1

32.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.384 ± 0.04

0.691 ± 0.011

4.823 ± 0.028

7.744 ± 0.044

4.722 ± 0.027

6.596 ± 0.037

2.062 ± 0.018

8.141 ± 0.041

6.93 ± 0.038

9.804 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.019

4.808 ± 0.029

3.498 ± 0.021

3.661 ± 0.024

3.846 ± 0.025

6.069 ± 0.03

5.571 ± 0.026

7.266 ± 0.033

1.052 ± 0.011

3.697 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski