Arthrobacter phage GurgleFerb

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Jasminevirus; unclassified Jasminevirus

Average proteome isoelectric point is 5.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XN57|A0A249XN57_9CAUD Portal protein OS=Arthrobacter phage GurgleFerb OX=2027884 GN=GURGLEFERB_13 PE=4 SV=1
MM1 pKa = 7.67EE2 pKa = 6.12DD3 pKa = 3.41EE4 pKa = 4.47NEE6 pKa = 4.04RR7 pKa = 11.84TPTMKK12 pKa = 10.74LLIEE16 pKa = 4.35GSKK19 pKa = 10.75VEE21 pKa = 4.21ITPDD25 pKa = 3.39TNASMYY31 pKa = 10.45EE32 pKa = 3.88YY33 pKa = 10.75LQDD36 pKa = 3.49NGLYY40 pKa = 10.34QLMEE44 pKa = 4.6EE45 pKa = 5.01GKK47 pKa = 8.55ATYY50 pKa = 8.92PIHH53 pKa = 6.84LPNGYY58 pKa = 9.05FVEE61 pKa = 4.67GVEE64 pKa = 4.62ISS66 pKa = 3.18

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XN87|A0A249XN87_9CAUD Uncharacterized protein OS=Arthrobacter phage GurgleFerb OX=2027884 GN=GURGLEFERB_48 PE=4 SV=1
MM1 pKa = 6.44EE2 pKa = 4.55QEE4 pKa = 4.22MFGAPIVRR12 pKa = 11.84WDD14 pKa = 3.68SLNPSAYY21 pKa = 9.94GRR23 pKa = 11.84VIVAGRR29 pKa = 11.84RR30 pKa = 11.84GGKK33 pKa = 6.39TQRR36 pKa = 11.84QRR38 pKa = 11.84EE39 pKa = 4.09WSSLLFDD46 pKa = 6.16EE47 pKa = 5.65ILEE50 pKa = 4.48TQNLMNKK57 pKa = 9.96AKK59 pKa = 10.11VRR61 pKa = 11.84DD62 pKa = 3.98YY63 pKa = 11.28IRR65 pKa = 11.84TQSPLAEE72 pKa = 4.07SVRR75 pKa = 11.84PNYY78 pKa = 9.5IRR80 pKa = 11.84SRR82 pKa = 11.84WVRR85 pKa = 11.84FANGHH90 pKa = 4.77GRR92 pKa = 11.84NNAVVVFSLSLDD104 pKa = 3.68VQGDD108 pKa = 3.55QQLIFTMDD116 pKa = 3.34QINRR120 pKa = 11.84LSTTRR125 pKa = 11.84EE126 pKa = 3.62TIYY129 pKa = 10.69RR130 pKa = 11.84LSALSVTIAGTPLMTSFILSLDD152 pKa = 3.2QLMEE156 pKa = 3.75KK157 pKa = 10.03RR158 pKa = 11.84GYY160 pKa = 8.05QFRR163 pKa = 11.84QKK165 pKa = 10.67VNPYY169 pKa = 9.68PLWQEE174 pKa = 3.97FF175 pKa = 3.37

Molecular weight: 20.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13902

38

1803

257.4

28.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.402 ± 0.492

0.575 ± 0.11

6.157 ± 0.19

7.172 ± 0.449

3.92 ± 0.21

7.294 ± 0.332

1.877 ± 0.128

5.395 ± 0.169

6.388 ± 0.354

7.344 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.87 ± 0.222

5.388 ± 0.237

4.568 ± 0.259

4.431 ± 0.28

4.64 ± 0.198

6.38 ± 0.316

6.028 ± 0.286

6.373 ± 0.23

1.446 ± 0.142

3.352 ± 0.241
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski