Gemmobacter caeni

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Gemmobacter

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T6B3A6|A0A2T6B3A6_9RHOB Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB OS=Gemmobacter caeni OX=589035 GN=C8N34_105185 PE=4 SV=1
MM1 pKa = 7.65RR2 pKa = 11.84FSLVCLALAGTTLPAAAEE20 pKa = 4.22SLSFTLSNQSNADD33 pKa = 3.18LTEE36 pKa = 5.45FYY38 pKa = 10.53ASPVGTEE45 pKa = 3.53SWEE48 pKa = 4.0EE49 pKa = 4.05NILSTGALPAGASGDD64 pKa = 3.5ITIAEE69 pKa = 4.33AKK71 pKa = 9.23GCEE74 pKa = 3.87YY75 pKa = 10.87DD76 pKa = 3.53FRR78 pKa = 11.84MVFADD83 pKa = 3.85GDD85 pKa = 4.05VLEE88 pKa = 5.25DD89 pKa = 3.97RR90 pKa = 11.84SNICDD95 pKa = 3.16SGAYY99 pKa = 8.66TIQQ102 pKa = 3.33

Molecular weight: 10.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T6B6B0|A0A2T6B6B0_9RHOB Beta-methylmalyl-CoA/L-malyl-CoA lyase OS=Gemmobacter caeni OX=589035 GN=C8N34_10374 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.39GGRR28 pKa = 11.84LVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5113

0

5113

1552672

29

2131

303.7

32.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.256 ± 0.052

0.89 ± 0.012

5.715 ± 0.031

5.749 ± 0.035

3.531 ± 0.02

9.02 ± 0.042

2.01 ± 0.017

4.752 ± 0.023

2.781 ± 0.026

10.426 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.694 ± 0.018

2.312 ± 0.019

5.457 ± 0.029

3.055 ± 0.02

7.433 ± 0.038

4.86 ± 0.026

5.389 ± 0.028

7.179 ± 0.03

1.454 ± 0.017

2.037 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski