Pseudomonas phage phCDa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Shizishanvirus; Pseudomonas virus phCDa

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5H901|A0A2Z5H901_9CAUD Uncharacterized protein OS=Pseudomonas phage phCDa OX=2268587 GN=phCDa_39 PE=4 SV=1
MM1 pKa = 7.76EE2 pKa = 4.54YY3 pKa = 11.26VLFNDD8 pKa = 5.61GYY10 pKa = 11.24VVAIDD15 pKa = 4.74DD16 pKa = 3.98SLEE19 pKa = 3.9YY20 pKa = 10.62SQLLAILTTVEE31 pKa = 4.27GAVGYY36 pKa = 8.01YY37 pKa = 8.09WKK39 pKa = 10.32PGSEE43 pKa = 4.1YY44 pKa = 10.49LWRR47 pKa = 11.84CISPLSGVNEE57 pKa = 3.94TTEE60 pKa = 4.05AVDD63 pKa = 3.42RR64 pKa = 11.84TKK66 pKa = 10.62NTVVDD71 pKa = 4.61EE72 pKa = 4.65PPDD75 pKa = 3.95VIKK78 pKa = 10.65LAIMLSS84 pKa = 3.62

Molecular weight: 9.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5H9X2|A0A2Z5H9X2_9CAUD Uncharacterized protein OS=Pseudomonas phage phCDa OX=2268587 GN=phCDa_42 PE=4 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84HH3 pKa = 6.46DD4 pKa = 3.61NVMDD8 pKa = 3.65VVSTNEE14 pKa = 3.69TSLLQMLAAHH24 pKa = 7.26PGSFVHH30 pKa = 6.33SNRR33 pKa = 11.84HH34 pKa = 4.36IIIRR38 pKa = 11.84KK39 pKa = 9.28RR40 pKa = 11.84FLLSYY45 pKa = 10.27GPQAHH50 pKa = 6.18NPKK53 pKa = 10.05QFEE56 pKa = 4.23CSVRR60 pKa = 11.84FVNEE64 pKa = 3.37PLEE67 pKa = 3.89ISRR70 pKa = 11.84RR71 pKa = 11.84RR72 pKa = 11.84IRR74 pKa = 11.84LVRR77 pKa = 11.84DD78 pKa = 3.39ALIKK82 pKa = 10.7

Molecular weight: 9.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

22187

45

3375

226.4

24.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.646 ± 0.734

0.928 ± 0.158

5.769 ± 0.152

6.436 ± 0.237

3.547 ± 0.148

6.855 ± 0.207

1.825 ± 0.168

4.904 ± 0.198

5.314 ± 0.247

9.023 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.146 ± 0.166

4.57 ± 0.173

4.029 ± 0.193

4.782 ± 0.249

4.881 ± 0.19

5.589 ± 0.265

6.193 ± 0.279

7.324 ± 0.211

1.294 ± 0.154

2.943 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski