Dictyobacter aurantiacus
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7362 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A401ZJZ6|A0A401ZJZ6_9CHLR Histidine kinase OS=Dictyobacter aurantiacus OX=1936993 GN=KDAU_45050 PE=4 SV=1
MM1 pKa = 7.47 SVTPSPTVVGVFRR14 pKa = 11.84 DD15 pKa = 3.58 RR16 pKa = 11.84 SVVEE20 pKa = 3.74 QVIEE24 pKa = 3.96 ALYY27 pKa = 10.34 QAGFAQDD34 pKa = 3.43 QIRR37 pKa = 11.84 YY38 pKa = 8.4 SVPDD42 pKa = 3.45 NTSGGFLNDD51 pKa = 3.7 LKK53 pKa = 11.28 HH54 pKa = 6.85 LFTGPNASGSGDD66 pKa = 3.69 LANDD70 pKa = 3.6 LTGMGLTSEE79 pKa = 4.21 QARR82 pKa = 11.84 YY83 pKa = 8.5 YY84 pKa = 10.86 ANEE87 pKa = 3.92 YY88 pKa = 11.07 DD89 pKa = 3.49 NGNIVMAVQALDD101 pKa = 3.88 NEE103 pKa = 4.34 QQAMNIFHH111 pKa = 6.51 QFGAYY116 pKa = 9.98 GIHH119 pKa = 5.22 NTLRR123 pKa = 11.84 EE124 pKa = 4.26 AEE126 pKa = 4.38 GMNEE130 pKa = 4.02 DD131 pKa = 4.0 AGYY134 pKa = 10.1 AQPQTDD140 pKa = 4.15 FNNPSAAYY148 pKa = 8.73 AQQSTDD154 pKa = 3.46 YY155 pKa = 8.91 QHH157 pKa = 6.62 QMSPYY162 pKa = 7.55 TQHH165 pKa = 7.11 PEE167 pKa = 4.64 DD168 pKa = 4.63 YY169 pKa = 9.34 AQQPASSTWGNNAEE183 pKa = 4.51 HH184 pKa = 6.24 GQPLNEE190 pKa = 4.17 EE191 pKa = 4.11 TNPRR195 pKa = 11.84 QEE197 pKa = 5.05 AVTNDD202 pKa = 3.07 QTAVHH207 pKa = 6.99 EE208 pKa = 4.23 YY209 pKa = 11.32 ANDD212 pKa = 3.34 NNYY215 pKa = 9.64 TYY217 pKa = 11.27 NDD219 pKa = 3.1 NAAYY223 pKa = 10.98 NNDD226 pKa = 2.8 NTAYY230 pKa = 9.97 NDD232 pKa = 3.46 SNAEE236 pKa = 4.05 YY237 pKa = 10.82 NEE239 pKa = 3.81 NNADD243 pKa = 3.63 YY244 pKa = 10.17 TEE246 pKa = 4.27 QNSTVAPAGAADD258 pKa = 4.87 QRR260 pKa = 11.84 DD261 pKa = 4.31 LPDD264 pKa = 4.28 NIQPEE269 pKa = 4.77 SVVDD273 pKa = 3.66 HH274 pKa = 5.51 QQNLPEE280 pKa = 4.51 DD281 pKa = 3.79 TTPIRR286 pKa = 11.84 EE287 pKa = 3.93 PSVSDD292 pKa = 3.25 EE293 pKa = 4.35 HH294 pKa = 8.38 LNAPDD299 pKa = 4.28 HH300 pKa = 6.42 QDD302 pKa = 3.37 QAAQDD307 pKa = 3.74 YY308 pKa = 9.61 RR309 pKa = 11.84 SPEE312 pKa = 4.12 TEE314 pKa = 4.73 DD315 pKa = 5.3 DD316 pKa = 3.75 PTADD320 pKa = 3.62 RR321 pKa = 11.84 QDD323 pKa = 3.73 YY324 pKa = 11.19 NSADD328 pKa = 3.67 NQGDD332 pKa = 3.49 ITDD335 pKa = 3.93 EE336 pKa = 4.14 TTQPVGSDD344 pKa = 3.38 DD345 pKa = 3.37 RR346 pKa = 11.84 AGAYY350 pKa = 7.78 RR351 pKa = 11.84 APATDD356 pKa = 4.26 SDD358 pKa = 4.23 TQNATSAPVIDD369 pKa = 4.45 GANRR373 pKa = 11.84 EE374 pKa = 4.22 YY375 pKa = 9.41 PTTVTYY381 pKa = 10.88 DD382 pKa = 3.56 EE383 pKa = 4.86 TPGTPAADD391 pKa = 3.85 NAPLDD396 pKa = 4.15 AQPANAVDD404 pKa = 3.45 NSTDD408 pKa = 3.32 NTTSTAQHH416 pKa = 6.41 TDD418 pKa = 2.82 TFEE421 pKa = 3.9 NAAYY425 pKa = 9.92 QSPATHH431 pKa = 6.34 NGEE434 pKa = 4.15 VATLSTNYY442 pKa = 8.43 NTEE445 pKa = 4.09 PDD447 pKa = 3.51 LSADD451 pKa = 3.18 QGQIDD456 pKa = 4.22 EE457 pKa = 5.47 ADD459 pKa = 3.34 HH460 pKa = 5.86 QQYY463 pKa = 7.53 QTAASTTEE471 pKa = 3.95 NAPLDD476 pKa = 3.96 QQQAPQDD483 pKa = 3.69 ATAGTTATDD492 pKa = 4.33 FAAQRR497 pKa = 11.84 EE498 pKa = 4.27 EE499 pKa = 4.44 RR500 pKa = 11.84 LRR502 pKa = 11.84 QLQQRR507 pKa = 11.84 LEE509 pKa = 4.16 SAKK512 pKa = 10.2 QRR514 pKa = 11.84 LQDD517 pKa = 4.09 SQTQLQAAKK526 pKa = 9.22 EE527 pKa = 4.25 HH528 pKa = 6.36 EE529 pKa = 4.47 SQLQSMKK536 pKa = 10.36 QQLDD540 pKa = 3.79 AIEE543 pKa = 5.32 AEE545 pKa = 4.28 LEE547 pKa = 4.13 KK548 pKa = 11.41 NEE550 pKa = 3.96 AEE552 pKa = 4.55 LRR554 pKa = 11.84 EE555 pKa = 3.92 THH557 pKa = 6.91 ARR559 pKa = 11.84 IEE561 pKa = 3.89 QYY563 pKa = 10.78 YY564 pKa = 10.14
Molecular weight: 61.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.923
IPC_protein 3.935
Toseland 3.732
ProMoST 4.075
Dawson 3.91
Bjellqvist 4.062
Wikipedia 3.821
Rodwell 3.757
Grimsley 3.63
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.24
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.05
Patrickios 1.291
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.944
Protein with the highest isoelectric point:
>tr|A0A401ZEE7|A0A401ZEE7_9CHLR Enterochelin esterase OS=Dictyobacter aurantiacus OX=1936993 GN=fes PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.45 RR3 pKa = 11.84 TWQPKK8 pKa = 8.26 RR9 pKa = 11.84 IPRR12 pKa = 11.84 KK13 pKa = 9.21 RR14 pKa = 11.84 EE15 pKa = 3.41 HH16 pKa = 7.15 GFMKK20 pKa = 10.47 RR21 pKa = 11.84 MSTRR25 pKa = 11.84 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLKK32 pKa = 10.49 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.41 GRR39 pKa = 11.84 WSLTVV44 pKa = 3.02
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.425
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.369
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.087
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7362
0
7362
2368895
39
4032
321.8
35.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.439 ± 0.029
0.978 ± 0.009
4.904 ± 0.022
5.77 ± 0.036
3.612 ± 0.02
7.149 ± 0.026
2.619 ± 0.014
5.853 ± 0.024
3.198 ± 0.021
10.847 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.349 ± 0.012
3.323 ± 0.022
5.168 ± 0.024
5.167 ± 0.023
6.238 ± 0.028
6.129 ± 0.026
5.858 ± 0.023
6.77 ± 0.027
1.494 ± 0.013
3.134 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here