Blattabacterium sp. subsp. Periplaneta americana (strain BPLAN) (Periplaneta americana symbiotic bacterium)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Blattabacteriaceae; Blattabacterium; unclassified Blattabacterium; Blattabacterium sp. (Periplaneta americana)

Average proteome isoelectric point is 8.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 581 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D0JA13|D0JA13_BLASP Methionyl-tRNA formyltransferase OS=Blattabacterium sp. subsp. Periplaneta americana (strain BPLAN) OX=600809 GN=fmt PE=3 SV=1
MM1 pKa = 7.19YY2 pKa = 7.77WTLEE6 pKa = 4.21LASHH10 pKa = 7.01LEE12 pKa = 4.08DD13 pKa = 4.93APWPATKK20 pKa = 10.57EE21 pKa = 3.95EE22 pKa = 5.15LIDD25 pKa = 3.5FAIRR29 pKa = 11.84TGAPLEE35 pKa = 4.22VVEE38 pKa = 5.79NLQQLEE44 pKa = 4.2NGEE47 pKa = 4.47GEE49 pKa = 4.28VFEE52 pKa = 4.88SIEE55 pKa = 4.99DD56 pKa = 3.07IWADD60 pKa = 3.63YY61 pKa = 10.07PRR63 pKa = 11.84DD64 pKa = 4.12DD65 pKa = 5.44EE66 pKa = 4.79DD67 pKa = 5.45FYY69 pKa = 11.33WNRR72 pKa = 11.84DD73 pKa = 3.3EE74 pKa = 5.55YY75 pKa = 11.35EE76 pKa = 3.85LL77 pKa = 5.44

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D0J8S8|D0J8S8_BLASP Uncharacterized protein OS=Blattabacterium sp. subsp. Periplaneta americana (strain BPLAN) OX=600809 GN=BPLAN_118 PE=3 SV=1
MM1 pKa = 7.82PNGKK5 pKa = 9.03KK6 pKa = 9.86RR7 pKa = 11.84KK8 pKa = 7.98RR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.52ISTHH15 pKa = 4.49KK16 pKa = 9.9RR17 pKa = 11.84KK18 pKa = 9.79KK19 pKa = 8.99RR20 pKa = 11.84NRR22 pKa = 11.84KK23 pKa = 8.44NRR25 pKa = 11.84HH26 pKa = 4.9KK27 pKa = 10.58KK28 pKa = 9.78KK29 pKa = 10.46KK30 pKa = 9.85

Molecular weight: 3.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

581

0

581

199588

30

1435

343.5

39.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.826 ± 0.087

1.007 ± 0.028

4.239 ± 0.052

6.356 ± 0.098

5.907 ± 0.099

5.803 ± 0.083

2.296 ± 0.041

11.085 ± 0.091

10.881 ± 0.111

9.332 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.255 ± 0.037

5.971 ± 0.069

3.286 ± 0.039

2.929 ± 0.049

3.82 ± 0.056

7.183 ± 0.072

4.097 ± 0.056

4.734 ± 0.069

0.879 ± 0.032

4.114 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski