Strawberry mild yellow edge-associated virus (SMYEaV)
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q00848|TGB3_SMYEA Movement protein TGBp3 OS=Strawberry mild yellow edge-associated virus OX=12187 GN=ORF4 PE=3 SV=1
MM1 pKa = 8.47 VEE3 pKa = 3.87 FTKK6 pKa = 10.79 RR7 pKa = 11.84 LLLEE11 pKa = 4.44 RR12 pKa = 11.84 NFEE15 pKa = 4.1 RR16 pKa = 11.84 TNRR19 pKa = 11.84 PITGPIVVHH28 pKa = 6.33 AVAGAGKK35 pKa = 10.02 SSVINTVSLTFQLICWTTLPEE56 pKa = 4.06 EE57 pKa = 4.33 KK58 pKa = 10.64 ASFNCLHH65 pKa = 6.46 LRR67 pKa = 11.84 HH68 pKa = 6.62 LDD70 pKa = 3.59 GPAFPGAFVDD80 pKa = 5.08 EE81 pKa = 4.46 YY82 pKa = 11.61 QLADD86 pKa = 3.54 TDD88 pKa = 4.0 LSEE91 pKa = 4.38 AAFLFGDD98 pKa = 4.15 PLQYY102 pKa = 10.1 PGPAAQVPHH111 pKa = 6.03 FVKK114 pKa = 10.61 LFSHH118 pKa = 6.69 RR119 pKa = 11.84 CGLNSASLIRR129 pKa = 11.84 EE130 pKa = 3.9 LGIAFEE136 pKa = 4.12 ASKK139 pKa = 10.81 LDD141 pKa = 3.92 SVQHH145 pKa = 6.45 LDD147 pKa = 4.18 PYY149 pKa = 11.25 SSDD152 pKa = 3.6 PEE154 pKa = 4.16 GTILAFEE161 pKa = 4.95 PEE163 pKa = 4.32 VQAALASHH171 pKa = 6.61 SLDD174 pKa = 3.66 FLCLDD179 pKa = 4.26 EE180 pKa = 5.84 FRR182 pKa = 11.84 GKK184 pKa = 9.04 QWPVCTLYY192 pKa = 11.21 VSTKK196 pKa = 10.11 NLCDD200 pKa = 3.92 LDD202 pKa = 4.25 RR203 pKa = 11.84 PSVYY207 pKa = 10.35 VALTRR212 pKa = 11.84 HH213 pKa = 5.36 YY214 pKa = 10.41 EE215 pKa = 3.84 RR216 pKa = 11.84 LLIMSFDD223 pKa = 3.99 AADD226 pKa = 3.57 TSAA229 pKa = 4.49
Molecular weight: 25.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.015
IPC2_protein 5.08
IPC_protein 5.029
Toseland 5.067
ProMoST 5.219
Dawson 5.08
Bjellqvist 5.194
Wikipedia 4.978
Rodwell 5.003
Grimsley 5.016
Solomon 5.08
Lehninger 5.041
Nozaki 5.232
DTASelect 5.397
Thurlkill 5.092
EMBOSS 5.067
Sillero 5.296
Patrickios 3.706
IPC_peptide 5.092
IPC2_peptide 5.296
IPC2.peptide.svr19 5.236
Protein with the highest isoelectric point:
>tr|Q88468|Q88468_SMYEA 11K protein OS=Strawberry mild yellow edge-associated virus OX=12187 PE=4 SV=1
MM1 pKa = 7.29 TLALHH6 pKa = 6.68 PLPNRR11 pKa = 11.84 SVLHH15 pKa = 6.12 PSAARR20 pKa = 11.84 ACKK23 pKa = 10.16 SPPSFEE29 pKa = 3.81 ITALSASSVCSTPQASGTKK48 pKa = 9.41 QSGTTVPLAIGAISSLHH65 pKa = 6.02 RR66 pKa = 11.84 RR67 pKa = 11.84 PSSLPLISSMVCSIQPAKK85 pKa = 10.37 KK86 pKa = 10.02 SRR88 pKa = 11.84 FGVSPPPRR96 pKa = 11.84 RR97 pKa = 11.84 FMPQPRR103 pKa = 11.84 IRR105 pKa = 11.84 MM106 pKa = 3.79
Molecular weight: 11.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.164
IPC2_protein 10.204
IPC_protein 11.74
Toseland 11.93
ProMoST 12.398
Dawson 11.93
Bjellqvist 11.915
Wikipedia 12.398
Rodwell 11.711
Grimsley 11.974
Solomon 12.413
Lehninger 12.31
Nozaki 11.93
DTASelect 11.915
Thurlkill 11.93
EMBOSS 12.427
Sillero 11.93
Patrickios 11.462
IPC_peptide 12.413
IPC2_peptide 11.403
IPC2.peptide.svr19 9.386
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2083
75
1323
347.2
38.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.785 ± 0.787
2.064 ± 0.496
5.713 ± 0.856
4.897 ± 1.034
4.273 ± 0.363
5.233 ± 0.425
2.784 ± 0.26
4.609 ± 0.477
5.473 ± 1.625
9.65 ± 0.681
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.488 ± 0.194
4.033 ± 0.593
7.489 ± 1.861
3.505 ± 0.323
5.377 ± 0.333
7.249 ± 1.519
6.097 ± 0.164
6.673 ± 0.18
1.344 ± 0.295
3.265 ± 0.509
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here