Callithrix jacchus (White-tufted-ear marmoset)
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 42838 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F7EM48|F7EM48_CALJA Isoform of F7I688 Solute carrier family 39 member 9 OS=Callithrix jacchus OX=9483 GN=SLC39A9 PE=4 SV=2
MM1 pKa = 7.51 SFLKK5 pKa = 10.56 SFPPPGPAEE14 pKa = 4.04 GLLRR18 pKa = 11.84 QQPDD22 pKa = 3.4 TEE24 pKa = 4.15 AVLNGKK30 pKa = 8.91 GLGTGTLYY38 pKa = 10.36 IAEE41 pKa = 4.77 SRR43 pKa = 11.84 LSWLDD48 pKa = 3.16 GSGLGFSLEE57 pKa = 4.09 YY58 pKa = 9.04 PTISLHH64 pKa = 5.94 ALSRR68 pKa = 11.84 DD69 pKa = 3.33 RR70 pKa = 11.84 SDD72 pKa = 4.01 CLGEE76 pKa = 3.91 HH77 pKa = 7.02 LYY79 pKa = 11.73 VMVNAKK85 pKa = 10.44 FEE87 pKa = 4.25 VEE89 pKa = 4.13 AMFTAMCEE97 pKa = 4.33 CQALHH102 pKa = 7.39 PDD104 pKa = 4.25 PEE106 pKa = 6.21 DD107 pKa = 4.16 EE108 pKa = 6.53 DD109 pKa = 5.1 SDD111 pKa = 5.45 DD112 pKa = 4.29 YY113 pKa = 11.91 DD114 pKa = 4.07 GEE116 pKa = 4.35 EE117 pKa = 4.51 YY118 pKa = 10.58 DD119 pKa = 5.25 VEE121 pKa = 4.24 AHH123 pKa = 5.97 EE124 pKa = 4.68 QGQGDD129 pKa = 3.76 IPTFYY134 pKa = 10.0 TYY136 pKa = 11.09 EE137 pKa = 4.17 EE138 pKa = 4.62 GLSHH142 pKa = 6.9 LTAEE146 pKa = 4.71 GQATLEE152 pKa = 4.16 RR153 pKa = 11.84 LEE155 pKa = 4.8 GMLSQSVSSQYY166 pKa = 11.68 NMAGVRR172 pKa = 11.84 TEE174 pKa = 4.15 DD175 pKa = 3.91 SIRR178 pKa = 11.84 DD179 pKa = 3.72 YY180 pKa = 11.39 EE181 pKa = 5.4 DD182 pKa = 3.26 GMEE185 pKa = 4.14 VDD187 pKa = 3.82 TTPTVAGQFEE197 pKa = 4.58 DD198 pKa = 4.85 ADD200 pKa = 3.71 VDD202 pKa = 3.98 HH203 pKa = 7.2
Molecular weight: 22.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.961
IPC_protein 3.935
Toseland 3.745
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.795
Rodwell 3.77
Grimsley 3.656
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.19
Thurlkill 3.77
EMBOSS 3.808
Sillero 4.05
Patrickios 1.214
IPC_peptide 3.897
IPC2_peptide 4.037
IPC2.peptide.svr19 3.937
Protein with the highest isoelectric point:
>tr|F6RYC6|F6RYC6_CALJA SNARE-associated protein Snapin OS=Callithrix jacchus OX=9483 GN=SNAPIN PE=2 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.12 WRR6 pKa = 11.84 KK7 pKa = 9.1 KK8 pKa = 9.32 RR9 pKa = 11.84 MRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 10.08 RR15 pKa = 11.84 KK16 pKa = 8.21 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.46 MRR21 pKa = 11.84 QRR23 pKa = 11.84 SKK25 pKa = 11.41
Molecular weight: 3.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22593
20245
42838
24220364
13
35445
565.4
63.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.885 ± 0.012
2.205 ± 0.011
4.824 ± 0.009
7.178 ± 0.016
3.611 ± 0.009
6.476 ± 0.017
2.605 ± 0.007
4.435 ± 0.01
5.864 ± 0.015
9.832 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.151 ± 0.006
3.659 ± 0.008
6.267 ± 0.019
4.77 ± 0.014
5.656 ± 0.011
8.38 ± 0.017
5.347 ± 0.012
5.975 ± 0.014
1.212 ± 0.004
2.632 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here