Clostridium sp. CAG:1219

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1041 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5THP8|R5THP8_9CLOT 30S ribosomal protein S17 OS=Clostridium sp. CAG:1219 OX=1262772 GN=rpsQ PE=3 SV=1
MM1 pKa = 7.09AQHH4 pKa = 6.89VIPITNGKK12 pKa = 7.09GTKK15 pKa = 9.44EE16 pKa = 4.07LTNGTYY22 pKa = 10.73NVTGEE27 pKa = 3.85FTGYY31 pKa = 10.34DD32 pKa = 3.37NSSILPTTQEE42 pKa = 3.2ISEE45 pKa = 4.35GVNAYY50 pKa = 10.59SFTIAATGNLTLHH63 pKa = 6.15ITDD66 pKa = 5.03DD67 pKa = 4.0GTNVGVPVEE76 pKa = 3.92GAIFIRR82 pKa = 11.84CDD84 pKa = 3.17AAGNTYY90 pKa = 10.32GNPVTSDD97 pKa = 2.57IDD99 pKa = 3.65GNAVFEE105 pKa = 4.67HH106 pKa = 6.53VPFAADD112 pKa = 3.2SAPAVYY118 pKa = 10.18YY119 pKa = 10.49KK120 pKa = 9.47QTASDD125 pKa = 4.34GEE127 pKa = 4.6HH128 pKa = 6.36EE129 pKa = 4.92FSDD132 pKa = 3.69EE133 pKa = 4.59LRR135 pKa = 11.84NTTLTEE141 pKa = 3.74EE142 pKa = 4.44TVTVEE147 pKa = 3.75IQNANSTEE155 pKa = 4.07RR156 pKa = 11.84TFTITDD162 pKa = 3.32ANYY165 pKa = 10.0EE166 pKa = 4.18NLPIADD172 pKa = 4.02GNITISGQQ180 pKa = 3.12

Molecular weight: 19.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5SZD9|R5SZD9_9CLOT Protein-export membrane protein SecF OS=Clostridium sp. CAG:1219 OX=1262772 GN=secF PE=3 SV=1
MM1 pKa = 7.49KK2 pKa = 10.4LSTSNPSNIKK12 pKa = 9.47RR13 pKa = 11.84KK14 pKa = 9.11KK15 pKa = 8.56VHH17 pKa = 6.13GFRR20 pKa = 11.84QRR22 pKa = 11.84MSTASGRR29 pKa = 11.84SVLKK33 pKa = 10.46ARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.34GRR40 pKa = 11.84KK41 pKa = 8.99VISAA45 pKa = 4.05

Molecular weight: 5.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1041

0

1041

289292

30

1769

277.9

31.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.068 ± 0.062

1.264 ± 0.031

5.678 ± 0.053

7.744 ± 0.082

4.152 ± 0.063

5.665 ± 0.076

1.06 ± 0.026

10.236 ± 0.085

10.221 ± 0.099

8.27 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.467 ± 0.034

6.864 ± 0.074

2.275 ± 0.04

2.236 ± 0.037

3.206 ± 0.049

6.683 ± 0.069

5.119 ± 0.073

6.681 ± 0.066

0.474 ± 0.019

4.629 ± 0.07
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski