Amino acid dipepetide frequency for Pseudomonas phage vB_PaeM_PAO1_Ab17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.277AlaAla: 8.277 ± 0.724
1.009AlaCys: 1.009 ± 0.225
5.168AlaAsp: 5.168 ± 0.548
5.451AlaGlu: 5.451 ± 0.533
2.342AlaPhe: 2.342 ± 0.286
6.501AlaGly: 6.501 ± 0.558
1.736AlaHis: 1.736 ± 0.226
3.957AlaIle: 3.957 ± 0.44
5.007AlaLys: 5.007 ± 0.661
7.752AlaLeu: 7.752 ± 0.702
3.351AlaMet: 3.351 ± 0.296
3.351AlaAsn: 3.351 ± 0.524
3.069AlaPro: 3.069 ± 0.311
4.199AlaGln: 4.199 ± 0.452
5.128AlaArg: 5.128 ± 0.447
4.966AlaSer: 4.966 ± 0.49
5.128AlaThr: 5.128 ± 0.591
6.218AlaVal: 6.218 ± 0.637
2.18AlaTrp: 2.18 ± 0.303
2.14AlaTyr: 2.14 ± 0.25
0.0AlaXaa: 0.0 ± 0.0
Cys
1.09CysAla: 1.09 ± 0.223
0.283CysCys: 0.283 ± 0.095
0.808CysAsp: 0.808 ± 0.21
1.09CysGlu: 1.09 ± 0.223
0.646CysPhe: 0.646 ± 0.178
1.252CysGly: 1.252 ± 0.269
0.363CysHis: 0.363 ± 0.104
0.525CysIle: 0.525 ± 0.119
0.525CysLys: 0.525 ± 0.16
0.888CysLeu: 0.888 ± 0.197
0.444CysMet: 0.444 ± 0.138
0.525CysAsn: 0.525 ± 0.143
0.767CysPro: 0.767 ± 0.179
0.808CysGln: 0.808 ± 0.209
1.05CysArg: 1.05 ± 0.189
0.848CysSer: 0.848 ± 0.211
0.646CysThr: 0.646 ± 0.167
0.525CysVal: 0.525 ± 0.147
0.444CysTrp: 0.444 ± 0.156
0.363CysTyr: 0.363 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
4.886AspAla: 4.886 ± 0.491
1.252AspCys: 1.252 ± 0.235
3.432AspAsp: 3.432 ± 0.354
4.764AspGlu: 4.764 ± 0.424
2.705AspPhe: 2.705 ± 0.329
4.522AspGly: 4.522 ± 0.459
1.332AspHis: 1.332 ± 0.239
4.118AspIle: 4.118 ± 0.403
3.109AspLys: 3.109 ± 0.377
5.007AspLeu: 5.007 ± 0.443
1.736AspMet: 1.736 ± 0.29
2.261AspAsn: 2.261 ± 0.338
2.18AspPro: 2.18 ± 0.296
1.978AspGln: 1.978 ± 0.237
3.513AspArg: 3.513 ± 0.409
2.665AspSer: 2.665 ± 0.342
3.23AspThr: 3.23 ± 0.365
4.24AspVal: 4.24 ± 0.364
1.292AspTrp: 1.292 ± 0.187
1.938AspTyr: 1.938 ± 0.325
0.0AspXaa: 0.0 ± 0.0
Glu
7.348GluAla: 7.348 ± 0.531
1.09GluCys: 1.09 ± 0.192
4.401GluAsp: 4.401 ± 0.437
6.299GluGlu: 6.299 ± 0.567
3.392GluPhe: 3.392 ± 0.315
5.693GluGly: 5.693 ± 0.521
1.211GluHis: 1.211 ± 0.207
3.351GluIle: 3.351 ± 0.339
4.482GluLys: 4.482 ± 0.527
6.379GluLeu: 6.379 ± 0.557
2.665GluMet: 2.665 ± 0.349
2.705GluAsn: 2.705 ± 0.39
2.261GluPro: 2.261 ± 0.295
3.351GluGln: 3.351 ± 0.395
3.674GluArg: 3.674 ± 0.361
3.715GluSer: 3.715 ± 0.346
2.665GluThr: 2.665 ± 0.331
6.501GluVal: 6.501 ± 0.585
1.454GluTrp: 1.454 ± 0.247
2.342GluTyr: 2.342 ± 0.269
0.0GluXaa: 0.0 ± 0.0
Phe
2.342PheAla: 2.342 ± 0.306
0.565PheCys: 0.565 ± 0.162
2.624PheAsp: 2.624 ± 0.359
3.392PheGlu: 3.392 ± 0.43
1.292PhePhe: 1.292 ± 0.227
2.342PheGly: 2.342 ± 0.306
0.727PheHis: 0.727 ± 0.17
1.736PheIle: 1.736 ± 0.233
2.221PheLys: 2.221 ± 0.326
3.513PheLeu: 3.513 ± 0.349
0.848PheMet: 0.848 ± 0.195
1.413PheAsn: 1.413 ± 0.219
2.261PhePro: 2.261 ± 0.283
1.696PheGln: 1.696 ± 0.282
1.777PheArg: 1.777 ± 0.278
2.705PheSer: 2.705 ± 0.303
1.978PheThr: 1.978 ± 0.302
1.978PheVal: 1.978 ± 0.274
0.485PheTrp: 0.485 ± 0.161
0.929PheTyr: 0.929 ± 0.167
0.0PheXaa: 0.0 ± 0.0
Gly
5.612GlyAla: 5.612 ± 0.524
0.727GlyCys: 0.727 ± 0.167
4.24GlyAsp: 4.24 ± 0.348
4.522GlyGlu: 4.522 ± 0.503
3.109GlyPhe: 3.109 ± 0.347
4.886GlyGly: 4.886 ± 0.502
1.373GlyHis: 1.373 ± 0.259
3.553GlyIle: 3.553 ± 0.393
4.038GlyLys: 4.038 ± 0.48
5.814GlyLeu: 5.814 ± 0.606
2.382GlyMet: 2.382 ± 0.387
3.109GlyAsn: 3.109 ± 0.406
2.18GlyPro: 2.18 ± 0.3
2.584GlyGln: 2.584 ± 0.365
3.715GlyArg: 3.715 ± 0.395
4.078GlySer: 4.078 ± 0.409
3.795GlyThr: 3.795 ± 0.609
5.37GlyVal: 5.37 ± 0.608
1.575GlyTrp: 1.575 ± 0.284
3.593GlyTyr: 3.593 ± 0.426
0.0GlyXaa: 0.0 ± 0.0
His
1.696HisAla: 1.696 ± 0.244
0.283HisCys: 0.283 ± 0.108
1.575HisAsp: 1.575 ± 0.265
1.615HisGlu: 1.615 ± 0.25
0.646HisPhe: 0.646 ± 0.182
1.777HisGly: 1.777 ± 0.261
0.283HisHis: 0.283 ± 0.095
1.05HisIle: 1.05 ± 0.192
0.848HisLys: 0.848 ± 0.205
1.978HisLeu: 1.978 ± 0.323
0.727HisMet: 0.727 ± 0.151
0.686HisAsn: 0.686 ± 0.161
1.292HisPro: 1.292 ± 0.236
0.686HisGln: 0.686 ± 0.199
1.211HisArg: 1.211 ± 0.226
0.969HisSer: 0.969 ± 0.211
1.09HisThr: 1.09 ± 0.226
1.171HisVal: 1.171 ± 0.23
0.444HisTrp: 0.444 ± 0.125
0.444HisTyr: 0.444 ± 0.122
0.0HisXaa: 0.0 ± 0.0
Ile
3.593IleAla: 3.593 ± 0.32
0.727IleCys: 0.727 ± 0.171
2.746IleAsp: 2.746 ± 0.32
3.755IleGlu: 3.755 ± 0.384
1.413IlePhe: 1.413 ± 0.255
3.19IleGly: 3.19 ± 0.419
0.969IleHis: 0.969 ± 0.196
2.221IleIle: 2.221 ± 0.281
2.261IleLys: 2.261 ± 0.318
4.563IleLeu: 4.563 ± 0.393
0.969IleMet: 0.969 ± 0.185
2.14IleAsn: 2.14 ± 0.321
3.069IlePro: 3.069 ± 0.412
2.14IleGln: 2.14 ± 0.25
2.544IleArg: 2.544 ± 0.406
2.463IleSer: 2.463 ± 0.286
3.432IleThr: 3.432 ± 0.402
2.907IleVal: 2.907 ± 0.314
0.727IleTrp: 0.727 ± 0.179
1.534IleTyr: 1.534 ± 0.246
0.0IleXaa: 0.0 ± 0.0
Lys
6.339LysAla: 6.339 ± 0.608
0.646LysCys: 0.646 ± 0.177
3.311LysAsp: 3.311 ± 0.439
4.603LysGlu: 4.603 ± 0.485
1.736LysPhe: 1.736 ± 0.288
4.24LysGly: 4.24 ± 0.429
1.413LysHis: 1.413 ± 0.259
2.584LysIle: 2.584 ± 0.377
3.795LysLys: 3.795 ± 0.475
5.007LysLeu: 5.007 ± 0.55
2.059LysMet: 2.059 ± 0.286
2.584LysAsn: 2.584 ± 0.334
1.817LysPro: 1.817 ± 0.284
2.019LysGln: 2.019 ± 0.263
3.149LysArg: 3.149 ± 0.406
2.584LysSer: 2.584 ± 0.377
2.544LysThr: 2.544 ± 0.316
4.643LysVal: 4.643 ± 0.539
0.929LysTrp: 0.929 ± 0.185
1.857LysTyr: 1.857 ± 0.311
0.0LysXaa: 0.0 ± 0.0
Leu
8.56LeuAla: 8.56 ± 0.728
1.332LeuCys: 1.332 ± 0.237
6.016LeuAsp: 6.016 ± 0.453
6.581LeuGlu: 6.581 ± 0.52
2.947LeuPhe: 2.947 ± 0.343
5.653LeuGly: 5.653 ± 0.536
1.494LeuHis: 1.494 ± 0.22
3.715LeuIle: 3.715 ± 0.422
5.451LeuLys: 5.451 ± 0.532
6.258LeuLeu: 6.258 ± 0.653
2.342LeuMet: 2.342 ± 0.272
3.593LeuAsn: 3.593 ± 0.4
4.24LeuPro: 4.24 ± 0.54
3.472LeuGln: 3.472 ± 0.351
5.895LeuArg: 5.895 ± 0.572
5.37LeuSer: 5.37 ± 0.458
4.764LeuThr: 4.764 ± 0.457
4.643LeuVal: 4.643 ± 0.426
1.171LeuTrp: 1.171 ± 0.187
3.27LeuTyr: 3.27 ± 0.376
0.0LeuXaa: 0.0 ± 0.0
Met
3.028MetAla: 3.028 ± 0.433
0.363MetCys: 0.363 ± 0.12
1.817MetAsp: 1.817 ± 0.217
2.423MetGlu: 2.423 ± 0.343
0.727MetPhe: 0.727 ± 0.139
1.817MetGly: 1.817 ± 0.305
0.565MetHis: 0.565 ± 0.138
1.534MetIle: 1.534 ± 0.303
2.301MetLys: 2.301 ± 0.314
2.261MetLeu: 2.261 ± 0.326
0.646MetMet: 0.646 ± 0.164
1.171MetAsn: 1.171 ± 0.285
1.413MetPro: 1.413 ± 0.27
1.171MetGln: 1.171 ± 0.212
1.615MetArg: 1.615 ± 0.284
2.1MetSer: 2.1 ± 0.31
1.817MetThr: 1.817 ± 0.273
1.696MetVal: 1.696 ± 0.254
0.363MetTrp: 0.363 ± 0.12
0.808MetTyr: 0.808 ± 0.216
0.0MetXaa: 0.0 ± 0.0
Asn
2.907AsnAla: 2.907 ± 0.378
0.444AsnCys: 0.444 ± 0.147
1.777AsnAsp: 1.777 ± 0.273
2.544AsnGlu: 2.544 ± 0.354
1.373AsnPhe: 1.373 ± 0.223
3.149AsnGly: 3.149 ± 0.375
1.09AsnHis: 1.09 ± 0.199
2.423AsnIle: 2.423 ± 0.272
2.301AsnLys: 2.301 ± 0.235
3.472AsnLeu: 3.472 ± 0.532
0.848AsnMet: 0.848 ± 0.175
1.575AsnAsn: 1.575 ± 0.293
2.503AsnPro: 2.503 ± 0.294
1.575AsnGln: 1.575 ± 0.357
2.019AsnArg: 2.019 ± 0.265
2.867AsnSer: 2.867 ± 0.389
2.221AsnThr: 2.221 ± 0.407
2.867AsnVal: 2.867 ± 0.279
0.525AsnTrp: 0.525 ± 0.174
1.332AsnTyr: 1.332 ± 0.219
0.0AsnXaa: 0.0 ± 0.0
Pro
3.634ProAla: 3.634 ± 0.453
0.606ProCys: 0.606 ± 0.173
2.261ProAsp: 2.261 ± 0.287
4.28ProGlu: 4.28 ± 0.412
1.777ProPhe: 1.777 ± 0.277
2.947ProGly: 2.947 ± 0.407
0.929ProHis: 0.929 ± 0.176
1.332ProIle: 1.332 ± 0.243
2.786ProLys: 2.786 ± 0.362
3.553ProLeu: 3.553 ± 0.407
0.929ProMet: 0.929 ± 0.181
1.777ProAsn: 1.777 ± 0.298
1.777ProPro: 1.777 ± 0.312
1.494ProGln: 1.494 ± 0.259
1.978ProArg: 1.978 ± 0.341
2.786ProSer: 2.786 ± 0.373
2.746ProThr: 2.746 ± 0.405
3.634ProVal: 3.634 ± 0.346
0.808ProTrp: 0.808 ± 0.18
1.413ProTyr: 1.413 ± 0.18
0.0ProXaa: 0.0 ± 0.0
Gln
4.805GlnAla: 4.805 ± 0.45
0.363GlnCys: 0.363 ± 0.125
1.978GlnAsp: 1.978 ± 0.272
3.351GlnGlu: 3.351 ± 0.329
1.534GlnPhe: 1.534 ± 0.239
2.584GlnGly: 2.584 ± 0.346
1.292GlnHis: 1.292 ± 0.227
1.817GlnIle: 1.817 ± 0.267
1.615GlnLys: 1.615 ± 0.358
3.109GlnLeu: 3.109 ± 0.371
1.696GlnMet: 1.696 ± 0.336
1.373GlnAsn: 1.373 ± 0.289
1.373GlnPro: 1.373 ± 0.289
2.1GlnGln: 2.1 ± 0.321
2.221GlnArg: 2.221 ± 0.254
1.777GlnSer: 1.777 ± 0.215
2.221GlnThr: 2.221 ± 0.271
3.553GlnVal: 3.553 ± 0.425
0.969GlnTrp: 0.969 ± 0.216
1.211GlnTyr: 1.211 ± 0.219
0.0GlnXaa: 0.0 ± 0.0
Arg
4.724ArgAla: 4.724 ± 0.432
1.009ArgCys: 1.009 ± 0.221
3.513ArgAsp: 3.513 ± 0.444
4.401ArgGlu: 4.401 ± 0.418
2.382ArgPhe: 2.382 ± 0.318
2.907ArgGly: 2.907 ± 0.273
1.171ArgHis: 1.171 ± 0.208
2.826ArgIle: 2.826 ± 0.275
3.593ArgLys: 3.593 ± 0.491
5.814ArgLeu: 5.814 ± 0.568
1.777ArgMet: 1.777 ± 0.231
2.261ArgAsn: 2.261 ± 0.272
1.978ArgPro: 1.978 ± 0.294
2.947ArgGln: 2.947 ± 0.278
3.432ArgArg: 3.432 ± 0.365
2.059ArgSer: 2.059 ± 0.244
3.392ArgThr: 3.392 ± 0.351
3.917ArgVal: 3.917 ± 0.407
0.848ArgTrp: 0.848 ± 0.174
1.898ArgTyr: 1.898 ± 0.265
0.0ArgXaa: 0.0 ± 0.0
Ser
3.917SerAla: 3.917 ± 0.429
0.888SerCys: 0.888 ± 0.206
2.907SerAsp: 2.907 ± 0.365
3.472SerGlu: 3.472 ± 0.359
2.261SerPhe: 2.261 ± 0.328
4.482SerGly: 4.482 ± 0.491
0.606SerHis: 0.606 ± 0.154
2.14SerIle: 2.14 ± 0.27
3.674SerLys: 3.674 ± 0.37
4.805SerLeu: 4.805 ± 0.422
1.655SerMet: 1.655 ± 0.3
2.14SerAsn: 2.14 ± 0.293
3.149SerPro: 3.149 ± 0.431
2.059SerGln: 2.059 ± 0.235
3.392SerArg: 3.392 ± 0.383
3.23SerSer: 3.23 ± 0.425
3.23SerThr: 3.23 ± 0.348
4.038SerVal: 4.038 ± 0.387
1.252SerTrp: 1.252 ± 0.212
2.342SerTyr: 2.342 ± 0.331
0.0SerXaa: 0.0 ± 0.0
Thr
4.845ThrAla: 4.845 ± 0.463
0.606ThrCys: 0.606 ± 0.139
3.392ThrAsp: 3.392 ± 0.381
3.23ThrGlu: 3.23 ± 0.356
1.938ThrPhe: 1.938 ± 0.299
3.957ThrGly: 3.957 ± 0.523
1.211ThrHis: 1.211 ± 0.2
2.947ThrIle: 2.947 ± 0.372
1.978ThrLys: 1.978 ± 0.283
5.814ThrLeu: 5.814 ± 0.579
1.494ThrMet: 1.494 ± 0.243
2.059ThrAsn: 2.059 ± 0.373
3.149ThrPro: 3.149 ± 0.342
2.14ThrGln: 2.14 ± 0.281
2.786ThrArg: 2.786 ± 0.305
3.432ThrSer: 3.432 ± 0.414
2.907ThrThr: 2.907 ± 0.403
4.32ThrVal: 4.32 ± 0.387
0.888ThrTrp: 0.888 ± 0.189
1.978ThrTyr: 1.978 ± 0.274
0.0ThrXaa: 0.0 ± 0.0
Val
5.451ValAla: 5.451 ± 0.589
0.767ValCys: 0.767 ± 0.177
3.795ValAsp: 3.795 ± 0.427
6.137ValGlu: 6.137 ± 0.492
3.028ValPhe: 3.028 ± 0.381
4.886ValGly: 4.886 ± 0.465
1.534ValHis: 1.534 ± 0.304
3.513ValIle: 3.513 ± 0.303
4.401ValLys: 4.401 ± 0.48
6.258ValLeu: 6.258 ± 0.512
2.059ValMet: 2.059 ± 0.231
2.746ValAsn: 2.746 ± 0.326
3.069ValPro: 3.069 ± 0.311
2.423ValGln: 2.423 ± 0.318
3.957ValArg: 3.957 ± 0.401
4.199ValSer: 4.199 ± 0.413
3.715ValThr: 3.715 ± 0.426
5.41ValVal: 5.41 ± 0.599
1.252ValTrp: 1.252 ± 0.207
2.382ValTyr: 2.382 ± 0.281
0.0ValXaa: 0.0 ± 0.0
Trp
1.373TrpAla: 1.373 ± 0.259
0.202TrpCys: 0.202 ± 0.093
1.413TrpAsp: 1.413 ± 0.248
1.211TrpGlu: 1.211 ± 0.198
0.606TrpPhe: 0.606 ± 0.153
1.252TrpGly: 1.252 ± 0.25
0.363TrpHis: 0.363 ± 0.108
0.808TrpIle: 0.808 ± 0.181
1.575TrpLys: 1.575 ± 0.24
2.019TrpLeu: 2.019 ± 0.319
0.444TrpMet: 0.444 ± 0.178
0.767TrpAsn: 0.767 ± 0.179
0.283TrpPro: 0.283 ± 0.106
0.525TrpGln: 0.525 ± 0.169
1.131TrpArg: 1.131 ± 0.214
1.09TrpSer: 1.09 ± 0.196
1.171TrpThr: 1.171 ± 0.215
1.009TrpVal: 1.009 ± 0.187
0.404TrpTrp: 0.404 ± 0.177
0.808TrpTyr: 0.808 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.503TyrAla: 2.503 ± 0.334
0.727TyrCys: 0.727 ± 0.158
2.988TyrAsp: 2.988 ± 0.303
1.857TyrGlu: 1.857 ± 0.324
0.969TyrPhe: 0.969 ± 0.197
2.221TyrGly: 2.221 ± 0.253
0.808TyrHis: 0.808 ± 0.17
1.211TyrIle: 1.211 ± 0.203
1.898TyrLys: 1.898 ± 0.254
2.503TyrLeu: 2.503 ± 0.345
0.525TyrMet: 0.525 ± 0.141
1.575TyrAsn: 1.575 ± 0.253
1.615TyrPro: 1.615 ± 0.299
1.575TyrGln: 1.575 ± 0.251
2.665TyrArg: 2.665 ± 0.351
1.736TyrSer: 1.736 ± 0.261
2.382TyrThr: 2.382 ± 0.258
2.342TyrVal: 2.342 ± 0.305
0.485TyrTrp: 0.485 ± 0.131
1.09TyrTyr: 1.09 ± 0.186
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 148 proteins (24768 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski