Squirrel monkey simian foamy virus
Average proteome isoelectric point is 6.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D5JWU9|D5JWU9_9RETR Bel2 OS=Squirrel monkey simian foamy virus OX=2170201 GN=bel2 PE=4 SV=1
MM1 pKa = 7.75 ASYY4 pKa = 8.21 KK5 pKa = 9.44 TQGSVKK11 pKa = 10.28 DD12 pKa = 4.02 DD13 pKa = 3.53 LVSFLQEE20 pKa = 4.26 FPLEE24 pKa = 4.47 DD25 pKa = 4.84 LDD27 pKa = 6.38 DD28 pKa = 4.49 IPTPAPCGMFEE39 pKa = 4.77 EE40 pKa = 5.01 YY41 pKa = 10.5 FPPTPMDD48 pKa = 3.8 PVAMGSEE55 pKa = 4.53 YY56 pKa = 11.01 NVAPLAEE63 pKa = 5.2 LPDD66 pKa = 3.63 QLLSVTFPASPASTEE81 pKa = 4.1 SSSEE85 pKa = 3.95 AGAQDD90 pKa = 3.54 YY91 pKa = 9.83 TGEE94 pKa = 4.05 GTAAGEE100 pKa = 4.47 APPEE104 pKa = 4.37 AGPSRR109 pKa = 11.84 SNSQPGGTPFFWLQEE124 pKa = 3.68 ARR126 pKa = 11.84 KK127 pKa = 9.96 RR128 pKa = 11.84 SDD130 pKa = 3.32 YY131 pKa = 11.39 DD132 pKa = 3.39 CWGLNYY138 pKa = 9.02 FFPKK142 pKa = 9.6 PGQRR146 pKa = 11.84 EE147 pKa = 3.96 FYY149 pKa = 9.15 IQRR152 pKa = 11.84 SILTTAGYY160 pKa = 10.43 RR161 pKa = 11.84 PDD163 pKa = 3.59 LLRR166 pKa = 11.84 YY167 pKa = 8.71 SAAGGWYY174 pKa = 9.36 PIILPHH180 pKa = 6.69 EE181 pKa = 4.55 GVLGNTFQVYY191 pKa = 7.45 YY192 pKa = 10.86 LCLKK196 pKa = 10.58 CGDD199 pKa = 4.35 EE200 pKa = 4.24 VWDD203 pKa = 3.97 PLLYY207 pKa = 10.78 LWDD210 pKa = 3.74 QDD212 pKa = 4.11 LLAFYY217 pKa = 9.81 RR218 pKa = 11.84 AWEE221 pKa = 4.34 TTPHH225 pKa = 5.94 TSSPLSVLRR234 pKa = 11.84 RR235 pKa = 11.84 HH236 pKa = 6.56 DD237 pKa = 3.51 ASCRR241 pKa = 11.84 YY242 pKa = 8.86 LHH244 pKa = 6.84 HH245 pKa = 6.59 NAYY248 pKa = 10.33 NYY250 pKa = 9.7 SEE252 pKa = 4.44 RR253 pKa = 11.84 PNIRR257 pKa = 11.84 KK258 pKa = 8.82 PRR260 pKa = 11.84 RR261 pKa = 11.84 DD262 pKa = 3.54 PGLRR266 pKa = 11.84 WCTGKK271 pKa = 10.23 RR272 pKa = 11.84 RR273 pKa = 11.84 LLRR276 pKa = 11.84 DD277 pKa = 3.07 SCEE280 pKa = 4.13 EE281 pKa = 3.89 LVHH284 pKa = 6.97 PGTLPNPTQWTQLTPSHH301 pKa = 5.89 MHH303 pKa = 6.59 PLAALSEE310 pKa = 4.18 LLQPEE315 pKa = 4.88 LQTSPSSPLEE325 pKa = 3.85 GTKK328 pKa = 9.89 KK329 pKa = 10.42 RR330 pKa = 11.84 KK331 pKa = 9.12 LAEE334 pKa = 3.85
Molecular weight: 37.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.02
IPC2_protein 5.156
IPC_protein 5.105
Toseland 5.105
ProMoST 5.194
Dawson 5.13
Bjellqvist 5.27
Wikipedia 5.016
Rodwell 5.054
Grimsley 5.054
Solomon 5.13
Lehninger 5.092
Nozaki 5.27
DTASelect 5.41
Thurlkill 5.13
EMBOSS 5.08
Sillero 5.347
Patrickios 3.503
IPC_peptide 5.143
IPC2_peptide 5.347
IPC2.peptide.svr19 5.259
Protein with the highest isoelectric point:
>tr|D5JWU7|D5JWU7_9RETR Env leader protein OS=Squirrel monkey simian foamy virus OX=2170201 GN=env PE=4 SV=1
MM1 pKa = 7.07 YY2 pKa = 10.27 QPQHH6 pKa = 5.04 QLQVEE11 pKa = 4.03 IHH13 pKa = 5.57 DD14 pKa = 4.15 QKK16 pKa = 11.51 LIGYY20 pKa = 7.56 WDD22 pKa = 3.58 TGAQITCIPQVYY34 pKa = 9.92 LEE36 pKa = 3.98 QEE38 pKa = 4.11 KK39 pKa = 10.6 PIGKK43 pKa = 8.74 HH44 pKa = 5.29 VIEE47 pKa = 4.39 TVNGKK52 pKa = 5.99 TQRR55 pKa = 11.84 DD56 pKa = 3.48 AYY58 pKa = 10.3 YY59 pKa = 10.11 IKK61 pKa = 10.95 LKK63 pKa = 10.84 INGKK67 pKa = 9.86 KK68 pKa = 9.61 IEE70 pKa = 4.29 TEE72 pKa = 4.26 VIPSPFSYY80 pKa = 11.33 ALITPNDD87 pKa = 3.78 IPWFKK92 pKa = 10.67 PGGIEE97 pKa = 4.09 LTEE100 pKa = 4.08 KK101 pKa = 11.09 LPIQDD106 pKa = 3.68 YY107 pKa = 10.68 KK108 pKa = 11.86 DD109 pKa = 4.32 NIVKK113 pKa = 10.43 RR114 pKa = 11.84 ADD116 pKa = 2.97 ITKK119 pKa = 9.66 EE120 pKa = 4.04 EE121 pKa = 4.09 KK122 pKa = 10.86 GMLYY126 pKa = 10.73 KK127 pKa = 10.92 LLDD130 pKa = 4.03 KK131 pKa = 11.01 YY132 pKa = 11.48 DD133 pKa = 4.5 PLWQQWEE140 pKa = 4.26 NQVGNRR146 pKa = 11.84 QITPHH151 pKa = 7.18 IIATGTINPKK161 pKa = 8.77 PQKK164 pKa = 9.83 QYY166 pKa = 10.99 HH167 pKa = 6.53 INPKK171 pKa = 9.83 AKK173 pKa = 9.83 PSIQIVINDD182 pKa = 3.98 LLKK185 pKa = 10.96 QGVLKK190 pKa = 10.16 QQNSIMNTPIYY201 pKa = 9.13 PVPKK205 pKa = 10.27 TEE207 pKa = 4.46 GKK209 pKa = 8.74 WRR211 pKa = 11.84 MVLDD215 pKa = 3.44 YY216 pKa = 10.78 RR217 pKa = 11.84 AVNKK221 pKa = 7.69 TIPLIAAQNQHH232 pKa = 5.68 SAGILTNLVRR242 pKa = 11.84 QKK244 pKa = 10.91 YY245 pKa = 9.88 KK246 pKa = 10.25 STIDD250 pKa = 3.45 LSNGFWAHH258 pKa = 7.32 PIDD261 pKa = 5.96 QDD263 pKa = 4.25 SQWITAFTWEE273 pKa = 4.26 GKK275 pKa = 9.52 QYY277 pKa = 10.6 VWTRR281 pKa = 11.84 LPQGFLNSPALFTADD296 pKa = 3.46 VVDD299 pKa = 4.6 LLKK302 pKa = 10.61 EE303 pKa = 3.89 IPNVNVYY310 pKa = 10.51 VDD312 pKa = 4.78 DD313 pKa = 4.94 IYY315 pKa = 11.87 VSTEE319 pKa = 3.76 TINQHH324 pKa = 5.31 FQVLDD329 pKa = 3.9 KK330 pKa = 10.91 IFQKK334 pKa = 10.73 LLQAGYY340 pKa = 10.27 VVSLKK345 pKa = 10.62 KK346 pKa = 10.87 SNLCRR351 pKa = 11.84 YY352 pKa = 7.37 EE353 pKa = 3.93 VTFLGFTISKK363 pKa = 9.18 YY364 pKa = 10.68 GRR366 pKa = 11.84 GLTEE370 pKa = 3.96 EE371 pKa = 4.25 FQEE374 pKa = 4.23 KK375 pKa = 9.93 LRR377 pKa = 11.84 NISPPNSLKK386 pKa = 10.33 QLQSILGLLNFARR399 pKa = 11.84 NFIPNFSEE407 pKa = 4.63 LIKK410 pKa = 10.29 PLYY413 pKa = 9.8 EE414 pKa = 5.22 LISTAQGQSISWEE427 pKa = 4.21 PKK429 pKa = 9.63 HH430 pKa = 6.86 SQALNNLIIALNHH443 pKa = 6.72 ADD445 pKa = 4.15 NLEE448 pKa = 3.94 QRR450 pKa = 11.84 NGEE453 pKa = 4.06 VPLVIKK459 pKa = 10.62 INASNTTGYY468 pKa = 10.17 IRR470 pKa = 11.84 FYY472 pKa = 10.64 NKK474 pKa = 9.8 NGKK477 pKa = 9.52 RR478 pKa = 11.84 PIAYY482 pKa = 9.25 ASHH485 pKa = 6.18 VFNHH489 pKa = 6.15 TEE491 pKa = 3.83 QKK493 pKa = 8.2 FTPVEE498 pKa = 3.96 KK499 pKa = 10.77 LLTTMHH505 pKa = 6.77 KK506 pKa = 10.56 AIIKK510 pKa = 10.58 GIDD513 pKa = 3.44 LAIGQPIEE521 pKa = 4.13 IYY523 pKa = 10.67 SPIVSMQKK531 pKa = 9.61 LQKK534 pKa = 8.97 ITLPEE539 pKa = 3.99 RR540 pKa = 11.84 KK541 pKa = 9.44 ALSTRR546 pKa = 11.84 WLSWLSYY553 pKa = 10.62 IEE555 pKa = 4.34 DD556 pKa = 3.54 PRR558 pKa = 11.84 FLFIYY563 pKa = 10.47 DD564 pKa = 3.53 KK565 pKa = 10.64 TLPDD569 pKa = 4.21 LKK571 pKa = 10.75 EE572 pKa = 3.99 MPPTQTDD579 pKa = 3.84 DD580 pKa = 3.98 YY581 pKa = 11.5 NPMLPLHH588 pKa = 6.26 QYY590 pKa = 10.62 LAVFYY595 pKa = 10.19 TDD597 pKa = 3.35 GSSIKK602 pKa = 10.93 SPDD605 pKa = 3.23 PTKK608 pKa = 9.81 THH610 pKa = 6.22 SSGMGIVQAIYY621 pKa = 10.41 EE622 pKa = 4.31 PNFQIKK628 pKa = 8.73 HH629 pKa = 4.41 QWSIPLGDD637 pKa = 3.74 HH638 pKa = 5.83 TAQYY642 pKa = 11.48 AEE644 pKa = 3.91 IAAVEE649 pKa = 4.35 FACKK653 pKa = 9.82 KK654 pKa = 9.64 ALQVTGPVLIVTDD667 pKa = 3.44 SDD669 pKa = 4.12 YY670 pKa = 11.49 VARR673 pKa = 11.84 SVNNEE678 pKa = 3.71 LNFWRR683 pKa = 11.84 SNGFVNNKK691 pKa = 9.32 KK692 pKa = 10.41 KK693 pKa = 9.96 PLKK696 pKa = 10.38 HH697 pKa = 5.16 ISKK700 pKa = 9.02 WKK702 pKa = 10.23 SISEE706 pKa = 4.05 SLLLHH711 pKa = 6.01 KK712 pKa = 10.64 NITIVHH718 pKa = 6.06 EE719 pKa = 4.78 PGHH722 pKa = 5.86 QPSSTSVHH730 pKa = 4.78 TQGNALADD738 pKa = 3.66 KK739 pKa = 10.63 LAVQGSYY746 pKa = 10.11 TINNITIKK754 pKa = 10.51 PSLDD758 pKa = 3.31 TEE760 pKa = 4.16 LRR762 pKa = 11.84 AVLEE766 pKa = 4.45 GKK768 pKa = 9.88 LPKK771 pKa = 10.33 GYY773 pKa = 9.49 PKK775 pKa = 10.38 NLKK778 pKa = 10.14 YY779 pKa = 10.49 EE780 pKa = 4.28 YY781 pKa = 10.1 NSPNLIVIRR790 pKa = 11.84 KK791 pKa = 7.34 EE792 pKa = 3.8 GQRR795 pKa = 11.84 IIPPLSDD802 pKa = 3.06 RR803 pKa = 11.84 PKK805 pKa = 10.33 LVKK808 pKa = 9.68 QAHH811 pKa = 5.72 EE812 pKa = 3.95 LAHH815 pKa = 6.27 TGRR818 pKa = 11.84 EE819 pKa = 4.12 ATLLRR824 pKa = 11.84 LQNQYY829 pKa = 7.67 WWPKK833 pKa = 6.64 MRR835 pKa = 11.84 KK836 pKa = 9.21 DD837 pKa = 3.41 VSHH840 pKa = 7.31 CLRR843 pKa = 11.84 TCMPCLQTNSTNLTTTRR860 pKa = 11.84 PFQQIRR866 pKa = 11.84 PSKK869 pKa = 10.36 PFDD872 pKa = 3.39 KK873 pKa = 11.06 YY874 pKa = 10.63 YY875 pKa = 10.25 IDD877 pKa = 4.52 YY878 pKa = 10.48 IGPLPPSEE886 pKa = 4.4 GYY888 pKa = 10.44 SYY890 pKa = 11.68 VLVVVDD896 pKa = 4.87 SATGFCWLYY905 pKa = 8.1 PTKK908 pKa = 10.71 APSTRR913 pKa = 11.84 ATVKK917 pKa = 10.57 SLNFLLGIAVPKK929 pKa = 10.15 ILHH932 pKa = 6.43 SDD934 pKa = 3.04 QGSAFTSSDD943 pKa = 3.03 FANWAKK949 pKa = 10.45 EE950 pKa = 3.91 KK951 pKa = 10.64 EE952 pKa = 4.05 ITLEE956 pKa = 3.94 FSTPYY961 pKa = 10.25 HH962 pKa = 5.98 PQSSGKK968 pKa = 8.29 VEE970 pKa = 4.21 RR971 pKa = 11.84 KK972 pKa = 8.28 NQEE975 pKa = 3.51 IKK977 pKa = 10.81 KK978 pKa = 10.1 LLTKK982 pKa = 10.75 LLVGRR987 pKa = 11.84 PAKK990 pKa = 9.64 WYY992 pKa = 8.75 PLIPSVQLALNNTYY1006 pKa = 10.44 SPKK1009 pKa = 10.24 IKK1011 pKa = 9.59 LTPHH1015 pKa = 5.61 QLLFGVDD1022 pKa = 3.69 GNIPFANSDD1031 pKa = 3.58 TLDD1034 pKa = 3.48 LKK1036 pKa = 11.06 RR1037 pKa = 11.84 EE1038 pKa = 4.05 EE1039 pKa = 4.2 EE1040 pKa = 4.07 LALLSEE1046 pKa = 4.19 IRR1048 pKa = 11.84 TTLSTVSPEE1057 pKa = 4.23 PFPSTAKK1064 pKa = 9.1 TWTPSVGLLVQEE1076 pKa = 4.17 RR1077 pKa = 11.84 VYY1079 pKa = 10.73 RR1080 pKa = 11.84 PSQLRR1085 pKa = 11.84 PKK1087 pKa = 8.48 WKK1089 pKa = 10.0 KK1090 pKa = 7.22 PTPILEE1096 pKa = 4.32 VLNEE1100 pKa = 3.99 RR1101 pKa = 11.84 TVVIDD1106 pKa = 3.26 NNGQRR1111 pKa = 11.84 RR1112 pKa = 11.84 TVSVDD1117 pKa = 2.81 NLKK1120 pKa = 8.82 YY1121 pKa = 9.39 TPHH1124 pKa = 6.08 QKK1126 pKa = 10.64 DD1127 pKa = 3.29 GEE1129 pKa = 4.71 TYY1131 pKa = 10.67 DD1132 pKa = 3.59 SSS1134 pKa = 3.64
Molecular weight: 129.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.723
IPC2_protein 8.741
IPC_protein 8.58
Toseland 9.326
ProMoST 9.107
Dawson 9.619
Bjellqvist 9.326
Wikipedia 9.794
Rodwell 9.897
Grimsley 9.706
Solomon 9.633
Lehninger 9.589
Nozaki 9.385
DTASelect 9.297
Thurlkill 9.443
EMBOSS 9.765
Sillero 9.545
Patrickios 4.711
IPC_peptide 9.619
IPC2_peptide 7.819
IPC2.peptide.svr19 7.755
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
3399
334
1134
679.8
77.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.707 ± 0.393
1.618 ± 0.482
4.237 ± 0.254
5.855 ± 0.519
3.001 ± 0.173
5.737 ± 0.562
2.354 ± 0.211
6.884 ± 0.811
6.002 ± 1.076
9.768 ± 0.456
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.618 ± 0.269
5.296 ± 0.709
7.502 ± 0.787
5.355 ± 0.288
4.766 ± 0.702
6.737 ± 0.328
6.826 ± 0.248
5.325 ± 0.298
2.295 ± 0.293
4.119 ± 0.291
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here