Porphyromonas levii

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1990 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8WNV5|A0A4Y8WNV5_9PORP Uncharacterized protein OS=Porphyromonas levii OX=28114 GN=E4P47_07160 PE=4 SV=1
GG1 pKa = 6.94LADD4 pKa = 4.68YY5 pKa = 9.5MIDD8 pKa = 3.68YY9 pKa = 10.84NLGRR13 pKa = 11.84TTQQIYY19 pKa = 8.37EE20 pKa = 4.23VKK22 pKa = 10.58KK23 pKa = 9.84IDD25 pKa = 3.54NDD27 pKa = 3.9YY28 pKa = 11.25FVDD31 pKa = 3.64NN32 pKa = 4.56

Molecular weight: 3.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8WNY3|A0A4Y8WNY3_9PORP Uncharacterized protein OS=Porphyromonas levii OX=28114 GN=E4P47_05350 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.19QPSVRR10 pKa = 11.84KK11 pKa = 9.51KK12 pKa = 8.78VNKK15 pKa = 9.52HH16 pKa = 4.01GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.12RR41 pKa = 11.84LTVSAA46 pKa = 4.69

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1990

0

1990

680190

13

1984

341.8

38.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.221 ± 0.05

0.832 ± 0.019

5.336 ± 0.039

7.055 ± 0.057

4.092 ± 0.032

7.049 ± 0.049

2.043 ± 0.026

6.677 ± 0.04

6.143 ± 0.049

9.789 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.691 ± 0.024

4.328 ± 0.044

3.916 ± 0.026

3.724 ± 0.036

5.208 ± 0.038

6.326 ± 0.047

5.51 ± 0.032

6.843 ± 0.042

1.108 ± 0.02

4.11 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski